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Quantitative detection of ING1 mRNA under different gene regulation based on molecular beacon

Chinese science Bulletin 2006年第17期51卷 2059-2064页

作者： LIU Bin1,2.3,4, WANG Kemin1,2.3,4, xiao Zhiqiang5,6, WANG Wei1,3,4, TAN Weihong1,2.3,4, SUN Yi5,6, TANG Hongxing1,3,4 & YANG xiaohai1,3,41. State Key Laboratory of Chemo/Biosensing and Chemometrics, Hu- nan {2., Changsha 410082. china 2. Life science and Biotechnology Institute, Hunan {2., Chang- sha 410082. china 3. Chemistry and Chemical Engineer College, Hunan {2., Changsha 410082. china 4. Hunan Engineering Research Center for Bio-Nanotechnology, Hunan {2., Changsha 410082. china 5. Medical Experiment Research Center, Xiangya Hospital, Central South {2., Changsha 410008, china 6. Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Changsha 410008, china State Key Laboratory of Chemo/Biosensing and Chemometrics Hunan University Changsha China Life science and Biotechnology Institute Hunan University Changsha China Chemistry and Chemical Engineer College Hunan University Changsha China Hunan Engineering Research Center for Bio-Nanotechnology Hunan University Changsha China Medical Experiment Research Center Xiangya Hospital Central South University Changsha China Key Laboratory of Cancer Proteomics of Chinese Ministry of Health Changsha China

The effects of drug treatment, gene transfection, and p53 RNA interference (RNAi) on level of ING1 mRNA in tumor cells were quantitatively detected with the help of ING1 molecular beacon (MB)/cRNA standard curve based on MB detection technology. Results showed that the level of ING1 mRNA could be upregulated by 5-FU treatment or ING1 transfection in low expression cell line of MCF-7 and be inhibited by silencing p53 with RNAi technology in normal expression cell line of CNE2. The level of ING1 varied from 7.7×10?16 to 53.4× 10?16 mol/μg total RNA of tumor cells. The results not only provided evidence for the regulation effects of gene expression but also were {2. to investigat- ing the interaction of multigenes on signal transduc- tion pathway.

关键词： ING1 RT-PCR 定量分析分子信号 mRNA

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An electromagnetic semi-active dynamic vibration absorber for thin-walled workpiece vibration suppression in mirror milling

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applied Mathematics and Mechanics(English Edition) 2024年第8期45卷 1315-1334页

作者： Jianghua KONG Bei DING Wei WANG Zhixia WANG Juliang xiao Hongyun QIU School of Mechanical Engineering Tianjin UniversityTianjin 300350China Tianjin Key Laboratory of Nonlinear Dynamics and Control Tianjin 300350China Key Laboratory of Modern Mechanisms and Equipment Design of the State Ministry of Education Tianjin UniversityTianjin 300350China

As critical components of aircraft skins and rocket fuel storage tank shells,large thin-walled workpieces are susceptible to vibration and deformation during machining due to their weak local *** address these challenges,we propose a novel tunable electromagnetic semi-active dynamic vibration absorber(ESADVA),which integrates with a magnetic suction follower to form a followed ESADVA(follow-ESADVA)for mirror *** system combines a tunable magnet oscillator with a follower,enabling real-time vibration absorption and condition feedback throughout the milling ***,the device supports self-sensing and frequency adjustment by providing feedback to a linear actuator,which alters the distance between *** resolves the traditional issue of being unable to directly monitor vibration at the machining point due to space constraints and tool *** frequency shift characteristics and vibration absorption performance are comprehensively *** and experimental results demonstrate that the prototyped follow-ESADVA achieves frequency synchronization with the milling tool,resulting in a vibration suppression rate of approximately 47.57%.Moreover,the roughness of the machined surface decreases by18.95%,significantly enhancing the surface *** results of this work pave the way for higher-quality machined surfaces and a more stable mirror milling process.

关键词： semi-active dynamic vibration absorber(SADVA) mirror milling selfsensing vibration absorption tuning thin-walled workpiece

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Research on Simulation of Information Sharing in Virtual Enterprise Based on Multi-Agent

Research on Simulation of Information Sharing in Virtual Ent...

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2.09中国控制与决策会议

作者： Yaofeng Zhang~(1,2.,Renbin xiao~1 1.Department of Control science and Engineering,Huazhong {2. of science and technology,Wuhan 430074,china 2.Department of Statistic and applied Mathematics,Hubei {2. of Economics,Wuhan 4302.5,china

＜正＞The simulation based on the Multi-Agent plays an important role in the research of *** paper formulates the game theory model of information sharing in virtual *** the computer simulation technology of Multi-Agent,we study the causes of difficulty in forming and maintaining the cooperation of information sharing in virtual *** results show that the sharing costs,the punitive coefficient and the initial rate of cooperation are the factors influencing the level of cooperation in information *** last,we put forward the corresponding measures of enhancing the level of cooperation.

关键词： Multi-Agent Virtual enterprise Information sharing Prisoner’s dilemma Simulation

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Syntheses of new di-nuclear and tetra-nuclear lanthanide 9-anthracenecarboxylates:Magnetocaloric effect,slow magnetic relaxation and luminescent properties

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Journal of Rare Earths 2024年第6期42卷 1101-1109,I0004页

作者： Xing Liu Ruonan Yang Lu Zhang Jian Zhou Chunmei Huang Huahong Zou Zunhong xiao Chongqing Key Laboratory of Inorganic Functional Materials College of ChemistryChongqing Normal UniversityChongqing401331China Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources(Ministry of Education of china) School of Chemistry and Chemical Engineering of Guangxi Normal UniversityGuilin541004China School of Chemistry and Materials Guizhou Normal UniversityGuiyang550025China

Lanthanide complex-based materials with magnetic and luminescent properties have received a great deal of attention,owing to their importantly potential applications in diverse *** this work,the 9-anthracenecarboxylic acid(9-Haca)as bridging ligand was applied in combination with optically activeπ-conjugated 2.4,6-tri(2.pyridyl)-1,3,5-triazine(tptz)to construct a series of new di-nuclear and tetranuclear lanthanide 9-anthracenecarboxylates[La_(2.(9-aca)_(6)(tptz)_(2.(μ-H_(2.O)]·0.2.H_(2.O(1),[Ln_(2.(9-aca)_(6)(tptz)_(2.(H_(2.O)_(2.]·0.5AQ·CH_(3)COOH_(2.H_(2.O[Ln=Sm(2.),Eu(2.),Gd(2.),Tb(2.),Dy(2.),Tm(2.);AQ=9,10-anthracenedione]and[Ln_(4)(9-aca)_(10)(tptz)_(2.(CH3COO)2.·H_(2.O[Ln=Eu(3a),Gd(3b),Dy(3c)].1 displays a di-nuclear centrosymmetric moiety[La_(2.(9-aca)_(6)(tptz)_(2.(μ-H_(2.O)]built up from two complex[La(9-aca)_(2.(tptz)]groups bridged by one H_(2.O molecule and two syn-syn-μ2.η1:η1-9-aca-bridging ligands,while 2.-2. are isostructural and co mprise another di-nuclear centrosymmetric moieties[Ln_(2.(9-aca)_(6)(tptz)_(2.(H_(2.O)_(2.]based on two complex[Ln(9-aca)_(2.(tptz)(H_(2.O)]groups connected by two syn-syn-μ2.η1:η1-9-aca-bridging ligands,3a-3c are isostructural and show tetra-nuclear centro symmetric moieties[Ln4(9-aca)10(tptz)2.CH3COO)2.containing three types of coordination modes of *** present compounds offer the only examples of lanthanide 9-anthracenecarboxylates decorated by optically active *** investigations of 2.,2.-2.,and 3b-3c indicate that 3c is typical of slow relaxation of the magnetization,while 2. and 3b show significant magnetocaloric effect(MCE)with the value of-ΔSm of 5.2. J/(kg·K)at 4 K and 15.65 J/(kg·K)at 2.K(ΔH=7 T),*** luminescent properties were also studied and reveal that the characteristic luminescent properties of the 7r-conjugated aromatic ligands are introduced into magnetic lanthanide 9-anthracenecarboxylates,simultaneously exhibiting slow magnetic relaxation(or MCE)and luminescen

关键词： Lanthanide complexes Luminescence Magnetic properties Magnetocaloric effect Slow magnetic relaxation Rare earths

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Wireless electrical stimulation generated by piezoelectric nanomaterial promotes the dental pulp regeneration via regulating mitochondrial Ca^(2.)/PKA signaling pathway

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Journal of Materials science & technology 2024年第1期168卷 24-34页

作者： Jianmao Zheng Jiyuan Zuo Cairong xiao Qining Guo xiaobin Fu Chengyun Ning Peng Yu Guangdong Provincial Key Laboratory of Stomatology Hospital of StomatologySun Yat-Sen UniversityGuangzhou 510080China School of Materials science and Engineering National Engineering Research Center for Tissue Restoration and ReconstructionSouth China University of TechnologyGuangzhou 510641China

The induction of dental pulp stem cells(DPSCs)into odontogenic differentiation is a cutting-edge method of dental pulp regeneration ***,it remains a challenge to develop biomaterials and ther-apies that can induce odontogenic ***,we propose a wireless electrical stimulation strat-egy to induce DPSCs odontogenic differentiation via K_(0.5)Na_(0.5)NbO_(3)piezoelectric nanoparticles(KNN)and polarized KNN(PKNN),which can be endocytosed by *** vitro,several odontogenic differentiation indexes were also increased in DPSCs treated with KNN and PKNN,and the increase was more obvious in the PKNN *** wireless electrical field promoted mitochondrial calcium concentration via mitochondrial calcium unidirectional transporter(MCU),increased the production of adenosine triphos-phate(ATP),and induced odontogenic differentiation through the activation of the cAMP-PKA signaling *** vivo,dental pulp-like tissue was induced by electrical stimulation wirelessly with KNN and PKNN,which was more clinically friendly compared with the wired device,and the induction was more obvious in the PKNN group consistent with in vitro *** conclusion,this work demonstrates the potential of PKNN as an effective stimulus that can induce odontogenic differentiation of DPSCs and be applied to dental pulp regeneration treatment.

关键词： Piezoelectric nanoparticles Dental pulp stem cells Odontogenic differentiation

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Hydrogen trapping and diffusion in polycrystalline nickel:The spectrum of grain boundary segregation

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Journal of Materials science & technology 2024年第6期173卷 225-236页

作者： Yu Ding Haiyang Yu Meichao Lin Michael Ortiz Senbo xiao Jianying He Zhiliang Zhang Department of Structural Engineering Norwegian University of Science and Technology(NTNU)Trondheim 7491Norway Division of applied Mechanics Department of Materials Science and EngineeringUppsala UniversityUppsala 75121Sweden Graduate Aerospace Laboratories California Institute of Technology1200 E.California Blvd.PasadenaCA 91125USA

Hydrogen as an interstitial solute at grain boundaries(GBs)can have a catastrophic impact on the mechanical properties of many *** the global research effort,the underlying hydrogen-GB interactions in polycrystals remain inadequately *** this study,using Voronoi tessellations and atomistic simulations,we elucidate the hydrogen segregation energy spectrum at the GBs of polycrystalline nickel by exploring all the topologically favorable segregation *** distinct peaks in the energy spectrum are identified,corresponding to different structural *** first peak(-0.2.5 eV)represents the most favorable segregation sites at GB core,while the second and third peaks account for the sites at GB *** incorporating a thermodynamic model,the spectrum enables the determination of the equilibrium hydrogen concentrations at GBs,unveiling a remarkable two to three orders of magnitude increase compared to the bulk hydrogen concentration reported in experimental *** identified structures from the GB spectrum exhibit vastly different behaviors in hydrogen segregation and diffusion,with the low-barrier channels inside GB core contributing to short-circuit diffusion,while the high energy gaps between GB and neighboring lattice serving as on-plane diffusion *** square displacement analysis further confirms the findings,and shows that the calculated GB diffusion coefficient is three orders of magnitude greater than that of *** present study has a significant implication for practical applications since it offers a tool to bridge the gap between atomic-scale interactions and macroscopic behaviors in engineering materials.

关键词： Hydrogen embrittlement Grain boundaries Impurity segregation Polycrystal Atomistic simulations

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All-optical nanoscale thermometry with silicon carbide color centers

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Photonics Research 2024年第8期12卷 1696-1702页

作者： CHENGYING LIU HAIBO HU ZHENGTONG LIU SHUMIN xiao JUNFENG WANG YU ZHOU QINGHAI SONG Ministry of Industry and Information technology Key Laboratory of Micro-Nano Optoelectronic Information System Guangdong Provincial Key Laboratory of Semiconductor Optoelectronic Materials and Intelligent Photonic SystemsHarbin Institute of TechnologyShenzhen 518055China Pengcheng Laboratory Shenzhen 518055China Quantum science Center of Guangdong-Hong Kong-Macao Greater Bay Area Shenzhen-Hong Kong International Science and Technology ParkShenzhen 518045China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China College of Physics Sichuan UniversityChengdu 610065China

All-optical thermometry plays a crucial role in precision temperature measurement across diverse *** defects in solids are one of the most promising sensors due to their excellent sensitivity,stability,and ***,it faces limitations,such as the microwave heating effect and the complexity of spectral *** these challenges,we introduce a novel approach to nanoscale optical thermometry using quantum defects in silicon carbide(SiC),a material compatible with complementary metal-oxide-semiconductor(CMOS)*** method leverages the intensity ratio between anti-Stokes and Stokes emissions from SiC color centers,overcoming the drawbacks of traditional techniques such as optically detected magnetic resonance(ODMR)and zero-phonon line(ZPL)*** technique provides a real-time,highly sensitive(1.06%K^(-1)),and diffraction-limited temperature sensing protocol,which potentially helps enhance thermal management in the future miniaturization of electronic components.

关键词： carbide optical centers

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In-situ additive manufacturing of high strength yet ductility titanium composites with gradient layered structure using N_(2.

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International Journal of Extreme Manufacturing 2024年第3期6卷 387-409页

作者： Yunmian xiao Changhui Song Zibin Liu Linqing Liu Hanxiang Zhou Di Wang Yongqiang Yang School of Mechanical and Automotive Engineering South China University of TechnologyGuangzhou 510641People’s Republic of China

It has always been challenging work to reconcile the contradiction between the strength and plasticity of titanium *** powder bed fusion(LPBF) is a convenient method to fabricate innovative composites including those inspired by gradient layered *** this work,we used LPBF to selectively prepare Ti N/Ti gradient layered structure(GLSTi)composites by using different N_(2.–Ar ratios during the LPBF *** systematically investigated the mechanisms of in-situ synthesis Ti N,high strength and ductility of GLSTi composites using microscopic analysis,TEM characterization,and tensile testing with digital image ***,a digital correspondence was established between the N_(2. concentration and the volume fraction of LPBF in-situ synthesized Ti *** results show that the GLSTi composites exhibit superior mechanical properties compared to pure titanium fabricated by LPBF under pure ***,the tensile strength of GLSTi was more than 1.5times higher than that of LPBF-formed pure titanium,reaching up to 1100 MPa,while maintaining a high elongation at fracture of 17%.GLSTi breaks the bottleneck of high strength but low ductility exhibited by conventional nanoceramic particle-strengthened titanium matrix composites,and the hetero-deformation induced strengthening effect formed by the Ti N/Ti layered structure explained its strength-plasticity balanced *** microhardness exhibits a jagged variation of the relatively low hardness of 2.5 HV0.2.for the pure titanium layer and a high hardness of 408 HV0.2.for the N_(2. in-situ synthesis *** study provides a new concept for the structure-performance digital customization of 3D-printed Ti-based composites.

关键词： laser powder bed fusion layered structure composites in-situ synthesis TiN strength-plasticity synergy

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Catch organism assemblages along artificial reefs area and adjacent waters in Haizhou Bay

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Acta Oceanologica Sinica 2024年第2期43卷 34-42页

作者： Shike Gao Bin Xie Chengyu Huang xiao Zhang Shuo Zhang Wenwen Yu College of Marine Living Resource sciences and Management Shanghai Ocean UniversityShanghai 201306China Jiangsu Research Institute of Marine Fisheries Nantong 226007China Third Institute of Oceanography Ministry of Natural ResourcesXiamen361005China Joint Laboratory for Monitoring and Conservation of Aquatic Living Resources In the Yangtze Estuary Shanghai 200000China

To better understand the community patterns mediated by connectivity in artificial reefs of coastal areas, it is necessary to understand the distribution and coexistence of organisms with artificial reefs area and adjacent waters. This study was conducted to examine main catches assemblages collected by trawls in Haizhou Bay,which included five habitats: the artificial reef area(AR), aquaculture area(AA), natural area(NA), estuary area(EA) and comprehensive effect area(CEA). The result shows that the total abundances of species in the five habitats were highly different(univariate PERMANOVA: P = 0.001, n = 2.), but some species were also unique in their habitat(e.g. Scapharca subcrenata and Glossaulax didyma in AA). The body size distribution of specific species between habitats are different. For Collichthys lucidus, their body size in AR(14.63 cm ± 1.64 cm) and EA(14.3 cm ± 0.85 cm) is higher than that in NA(10.65 cm ± 1.64 cm), CEA(11.2. cm ± 1.85 cm) and AA(12.1 cm ±0.43 cm), which indicates the potential connection from AR to EA mediated by their adult population. We concluded that artificial reefs in AR can be considered key components that have the ability to support species assemblages in adjacent habitats. This study has implications for the conservation and monitoring of species assemblages in coastal areas in terms of that artificial reefs can be applied in different stages of habitat protection implementation and in different combinations of scenarios.

关键词： assemblage artificial reefs adjacent water Haizhou Bay

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HIV-1跨膜蛋白gp41与N-取代吡咯衍生物的结合模式研究(英文)

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生物化学与生物物理进展 2010年第8期37卷 904-915页

作者：丛肖静谭建军刘明陈慰祖王存新北京工业大学生命科学与生物工程学院北京100124

采用分子对接,分子动力学(MD)模拟和分子力学/泊松-波尔兹曼溶剂可有面积方法与分子力学/广义伯恩溶剂可及面积方法(MM-PBSA/MM-GBSA),预测两种N-取代吡咯衍生物与HIV-1跨膜蛋白gp41疏水口袋的结合模式与作用机理.分子对接采用多种受体... 详细信息

采用分子对接,分子动力学(MD)模拟和分子力学/泊松-波尔兹曼溶剂可有面积方法与分子力学/广义伯恩溶剂可及面积方法(MM-PBSA/MM-GBSA),预测两种N-取代吡咯衍生物与HIV-1跨膜蛋白gp41疏水口袋的结合模式与作用机理.分子对接采用多种受体构象,并从结果中选取几种可能的结合模式进行MD模拟,然后通过MM-PBSA计算结合能的方法识别最优的结合模式.MM-PBSA计算结果表明,范德华相互作用是结合的主要驱动力,而极性相互作用决定了配体在结合过程中的取向.进一步的结合能分解显示,配体的羧基与gp41残基Arg579的静电相互作用对结合有重要贡献.上述工作为进一步优化N-取代吡咯衍生物类的HIV-1融合抑制剂建立了良好的理论基础。

关键词： HIV-1融合抑制剂跨膜蛋白gp41 分子对接分子动力学模拟 MM-PBSA/MM-GBSA

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