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Improvement in Tol2 transposon for efficient large-cargo capacity transgene applications in cultured cells and zebrafish(Danio rerio)

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Zoological Research 2024年第3期45卷 567-574页

作者： Peng-Cheng Wang Hao Deng Rang Xu jiu-lin du Rongkun Tao Department of Pediatric Cardiology Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghai 200092China Institute of Neuroscience State Key Laboratory of NeuroscienceCenter for Excellence in Brain Science and Intelligence TechnologyChinese Academy of SciencesShanghai 200031China Medical School Kunming University of Science and TechnologyKunmingYunnan 650500China Scientific Research Center Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghai 200092China University of Chinese Academy of Sciences Beijing 100049China School of Life Science and Technology ShanghaiTech UniversityShanghai 200031China

Most viruses and transposons serve as effective carriers for the introduction of foreign DNA up to 11 kb into vertebrate ***,their activity markedly diminishes with payloads exceeding 11 *** the payload capacity of transposons could facilitate more sophisticated cargo designs,improving the regulation of expression and minimizing mutagenic risks associated with molecular therapeutics,metabolic engineering,and transgenic animal *** this study,we improved the Tol2 transposon by increasing protein expression levels using a translational enhancer(QBI SP163,ST)and enhanced the nuclear targeting ability using the nuclear localization protein H2B(SHT).The modified Tol2 and ST transposon efficiently integrated large DNA cargos into human cell cultures(H1299),comparable to the well-established super PiggyBac ***,mRNA from ST and SHT showed a significant increase in transgene delivery efficiency of large DNA payloads(8 kb,14 kb,and 24 kb)into zebrafish(Danio rerio).This study presents a modified Tol2 transposon as an enhanced nonviral vector for the delivery of large DNA payloads in transgenic applications.

关键词： Zebrafish Tol2 transposase Transgene Large payload Synthetic biology

Advancing neuroscience through real-time processing of big data: Transition from open-loop to closed-loop paradigms

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Improvement in Tol2 transposon for efficient large-cargo capacity transgene applications in cultured cells and zebrafish (Danio rerio)

动物学研究 2024年第3期 518页

作者： Yu-Fan Wang jiu-lin du

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durable dual-state duplex Si-HfO_(2)with excellent oxidation and cracking resistance

动物学研究 2024年第3期 567页

作者： Peng-Cheng Wang Hao Deng Rang Xu jiu-lin du Rongkun Tao

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Journal of Advanced Ceramics 2024年第3期13卷 388-401页

作者： lin Chen Jing-Chuan Luo Wen-Qi Yang Chang-jiu Li Guan-Jun Yang State Key Laboratory for Mechanical Behavior of Materials School of Materials Science and EngineeringXi’an Jiaotong UniversityXi’an 710049China Xi’an Aerospace Engine Company Limited in CASC Xi’an 710065China

The lifetime of Si bond coatings in environmental barrier coatings is constrained by phase-transition-induced cracking of the SiO_(2)*** this study,Si-HfO_(2)dual-state duplex composite materials are proposed to address this issue by partially forming HfSiO_(4)and minimizing the SiO_(2)*** as-prepared composite exhibited a structure comprising discrete HfO_(2)“bricks”embedded in a continuous Si“mortar”,while the oxidized state transformed into discrete HfSiO_(4)“bricks”within continuous thin SiO_(2)“mortars”.The results indicate that continuous thin SiO_(2)contributes to reducing the oxidation rate to a level comparable to that of pure Si,and discrete HfSiO_(4)particles aid in relieving phase transition-induced stress and inhibiting crack propagation,thereby enhancing oxidation and cracking resistance ***,the composite with 20 mol%HfO_(2)and a mean particle size of~500 nm at 1370℃exhibited a service lifetime 10 times greater than that of pure *** research provides valuable insights for designing Si-based bond coatings with improved service lifetime.

关键词： oxidation environmental barrier coatings(EBCs) phase transition cracking bond coatings

One-step generation of zebrafish carrying a conditional knockoutknockin visible switch via CRISPR/Cas9-mediated intron targeting

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Science China(Life Sciences) 2020年第1期63卷 59-67页

作者： Jia Li Hong-Yu Li Shan-Ye Gu Hua-Xing Zi Lai Jiang jiu-lin du Institute of Neuroscience State Key Laboratory of NeuroscienceCAS Center for Excellence in Brain Science and Intelligence TechnologyShanghai Research Center for Brain Science and Brain-Inspired IntelligenceChinese Academy of SciencesShanghai 200031China University of Chinese Academy of Sciences Beijing 100049China Department of Anesthesiology and Surgical Intensive Care Unit Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghai 200092China School of Life Science and Technology Shanghai Tech UniversityShanghai 200031China

The zebrafish has become a popular vertebrate animal model in biomedical ***,it is still challenging to make conditional gene knockout(CKO)models in zebrafish due to the low efficiency of homologous recombination(HR).Here we report an efficient non-HR-based method for generating zebrafish carrying a CKO and knockin(KI)switch(zCKOIS)coupled with dual-color fluorescent *** this strategy,we generated hey2^zCKOIS which served as a hey2 KI reporter with EGFP *** Cre induction in targeted cells,the hey2^zCKOIS was switched to a non-functional CKO allele hey2^zCKOIS-inv associated with Tag RFP expression,enabling visualization of the CKO ***,simplification of the design,and the visibility and combination of both CKO and KI alleles make our z CKOIS strategy an applicable CKO approach for zebrafish.

关键词： NHEJ non-HR knockin conditional knockout visible switch zCKOIS zebrafish

Process intensification for the synthesis and purification of battery-grade Li_(2)CO_(3)with microfluidics

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Particuology 2024年第7期90卷 106-117页

作者： Haodu Li Jie Zhang lin Dai Le du Jiqin Zhu The State Key Laboratory of Chemical Resource Engineering College of Chemical EngineeringBeijing University of Chemical TechnologyBeijing 100029China

Battery-grade lithium carbonate(Li_(2)CO_(3))with a purity of higher than 99.5 wt%is of great importance as a high value-added lithium ***,influences of different reaction systems and process control on product purity remain ***,a membrane dispersion microreactor was used to enhance the mass transfer of preparation and purification processes in homogeneous and heterogeneous *** systems of Na_(2)CO_(3)–LiCl,NH_(4)HCO_(3)–LiCl,and NH_(3)·H_(2)O−CO_(2)−LiCl,CO_(2)purification based on carbonation and decomposition were *** Li_(2)CO_(3)purity was increased by the improvement of mixing *** carbonation time was reduced by 62.5%and 58.3%for the NH_(3)·H_(2)O−CO_(2)and CO_(2)purification systems,*** the two ammonia-based systems,Li_(2)CO_(3)particles with a purity of 99.7–99.8 wt%were one-step prepared with a size of 3–5μm,which also met the requirement of the battery-grade *** purity was further increased to 99.9 wt%by CO_(2)purification and LiHCO_(3)*** investigation could provide a feasible alternative for the controllable preparation of battery-grade Li_(2)CO_(3)in one or multiple steps.

关键词： Microfluidics Battery grade Li_(2)CO_(3) Process intensification High purity

Sexual Dimorphism and Female Reproductive Characteristics in the Hainan Water Skink(Tropidophorus hainanus)from Hainan,South China

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Asian Herpetological Research 2024年第1期15卷 49-58页

作者： Yu du Xiaming ZHU Chixian lin Yuntao YAO Xiang JI Hainan Key Laboratory for Herpetological Research College of Fisheries and Life SciencesHainan Tropical Ocean UniversitySanya 572022HainanChina Zhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection College of Life and Environmental SciencesWenzhou UniversityWenzhou 325035ZhejiangChina Institute for Eco-environmental Research of Sanyang Wetland Wenzhou UniversityWenzhou 325014ZhejiangChina

We studied sexual dimorphism in body size and shape and female reproductive characteristics in the Hainan Water Skink(Tropidophorus hainanus)from Hainan,South *** our sample the largest female and male were 61.1 and 55.2 mm snout-vent length(SVL),*** mean SVL was larger in adult females(52.0 mm)than in adult males(48.3 mm).Juveniles were sexually dimorphic in head length but not in other examined morphological *** males were longer in head length and shorter in abdomen length and fore-limb length than adult females of the same *** shifts in sexual dimorphism in body shape were evident,as revealed by the fact that morphological differences between the sexes were more pronounced in adults than in *** produced a single litter of 3–6 offspring per season from early August to early *** size,litter mass and offspring(neonate)mass were positively related to female *** mass was independent of relative *** the above findings we draw three main ***,females are the larger sex in ***,and sexual dimorphism in body shape is more pronounced in adults than in ***,larger female *** produce more and larger offspring and thus heavier litters than smaller ***,the offspring size-number trade-off does is not evident in ***.

关键词： fecundity morphology offspring size-number trade-off reproduction scincidae sexual dimorphism

Filtration performance and modeling of granular bed for dust removal from coal pyrolytic vapors

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Chinese Journal of Chemical Engineering 2024年第1期65卷 35-42页

作者： Shuaiqiang Yang lin du Guangchao Ding Runguo Liu Wenli Song Songgeng Li State Key Laboratory of Multiphase Complex Systems Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China School of Petrochemical Engineering Yan’an Vocational and Technical CollegeYan’an 716000China Sino-Danish College University of Chinese Academy of SciencesBeijing 100049China

dust removal from pyrolytic vapors at high temperatures is an obstacle to the industrialization of the coal pyrolysis *** this work,a granular bed with expanded perlites as filtration media was designed and integrated into a 10 t·d^(–1)coal pyrolysis *** testing results showed that around 97.56%dust collection efficiency was *** a result,dust content in tar was significantly *** pressure drop of the granular bed maintained in the range of 356 Pa to 489 *** dust size in the effluent after filtration exhibited a bimodal distribution,which was attributed to the heterogeneity of the dust *** effects of filtration bed on pyrolytic product yields were also discussed.A modified filtration model based on the macroscopic phenomenological theory was proposed to describe the performance of the granular *** computation results were well agreed with the experimental data.

关键词： Granular bed filtration dust removal Pyrolysis Filtration Fixed-bed

Assembly of highly efficient aqueous light-harvesting system from sequence-defined peptoids for cytosolic microRNA detection

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Nano Research 2024年第2期17卷 788-796页

作者： Yang Song Xiaoli Cai Mingming Wang Dan du Yuehe lin Chun-Long Chen Department of Mechanical and Materials Engineering Washington State UniversityPullmanWA 99164USA Division of Physical Sciences Pacific Northwest National LaboratoryRichlandWA 99352USA Department of Chemical Engineering University of WashingtonSeattleWA 98195USA

Precisely controlled spatial distributions of artificial light-harvesting systems in aqueous media are of significant importance for mimicking natural light-harvesting systems;however,they are often restrained by the solubility and the aggregation-caused quenching effect of the hydrophobic ***,we report one highly efficient artificial light-harvesting system based on peptoid nanotubes that mimic the hierarchical cylindrical structure of natural *** high crystallinity of these nanotubes enabled the organization of arrays of donor chromophores with precisely controlled spatial distributions,favoring an efficient Förster resonance energy transfer(FRET)process in aqueous *** FRET system exhibits an extremely high efficiency of 98.6%with a fluorescence quantum yield of 40%and an antenna effect of *** further demonstrated the use of this artificial light-harvesting system for quantifying miR-210 within cancer *** fluorescence intensity ratio of donor to acceptor is linearly related to the concentration of intercellular miR-210 in the range of 3.3–156 copies/*** high sensitivity in intracellular detection of miR-210 using this artificial light-harvesting system offers a great opportunity and pathways for biological imaging and detection,and for the further creation of microRNA(miRNA)toolbox for quantitative epigenetics and personalized medicine.

关键词： light-harvesting peptoid nanotube Förster resonance energy transfer intracellular RNA detection