检索结果-南通市图书馆

Osteogenic differentiation of encapsulated cells in dexamethasone-loaded phospholipid-induced silk fibroin hydrogels

引用

Biomaterials Translational 2022年第3期3卷 213-220页

作者： Chavee Laomeephol Helena Ferreira Sorada Kanokpanont jittima amie luckanagul Nuno M Neves Siriporn Damrongsakkul Department of Pharmaceutics and Industrial Pharmacy Faculty of Pharmaceutical SciencesChulalongkorn UniversityBangkokThailand Biomaterial Engineering for Medical and Health Research Unit Chulalongkorn UniversityBangkokThailand B’s Research Group I3Bs-Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineGuimarãesPortugal ICVS/3B’s-PT Government Associate Laboratory Braga/GuimarãesPortugal Department of Chemical Engineering Faculty of EngineeringChulalongkorn UniversityBangkokThailand Biomedical Engineering Research Center Faculty of EngineeringChulalongkorn UniversityBangkokThailand Center of Excellence in Plant-produced Pharmaceuticals Chulalongkorn UniversityBangkokThailand

The tissue engineering triad comprises the combination of cells,scaffolds and biological factors.Therefore,we prepared cell-and drug-loaded hydrogels using in situ silk fibroin(SF)hydrogels induced by dimyristoyl glycerophosphoglycerol(DMPG).DMPG is reported to induce rapid hydrogel formation by SF,facilitating cell encapsulation in the hydrogel matrix while maintaining high cell viability and proliferative capacity.In addition,DMPG can be used for liposome formulations in entrapping drug molecules.Dexamethasone(Dex)was loaded into the DMPG-induced SF hydrogels together with human osteoblast-like SaOS-2 cells,then the osteogenic differentiation of the entrapped cells was evaluated in vitro and compared to cells cultured under standard conditions.Calcium production by cells cultured in DMPG/Dex-SF hydrogels with Dex-depleted osteogenic medium was equivalent to that of cells cultured in conventional osteogenic medium containing Dex.The extended-release of the entrapped Dex by the hydrogels was able to provide a sufficient drug amount for osteogenic induction.The controlled release of Dex was also advantageous for cell viability even though its dose in the hydrogels was far higher than that in osteogenic medium.The results confirmed the possibility of using DMPG-induced SF hydrogels to enable dual cell and drug encapsulation to fulfil the practical applications of tissue-engineered constructs.

关键词： DMPG osteogenic differentiation silk fibroin three-dimensional cell culture tissue engineering

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hyaluronic acid-based hydrogels with tobacco mosaic virus containing cell adhesive peptide induce bone repair in normal and osteoporotic rats

引用

Biomaterials Translational 2020年第1期1卷 89-98页

作者： Jishan Yuan Panita Maturavongsadit Zhihui Zhou Bin Lv Yuan Lin Jia Yang jittima amie luckanagul Department of Orthopaedic Surgery the Affiliated First People’s Hospital to Jiangsu UniversityZhenjiangJiangsu ProvinceChina Department of Chemistry and Biochemistry University of South CarolinaColumbiaSCUSA Joint Department of Biomedical Engineering North Carolina State University and The University of North Carolina at Chapel HillChapel HillNCUSA The State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchunJilin ProvinceChina Department of Pharmaceutics and Industrial Pharmacy Faculty of Pharmaceutical SciencesChulalongkorn UniversityBangkokThailand Research Unit for Plant-produced Pharmaceuticals Chulalongkorn UniversityBangkokThailand.

Tobacco mosaic virus(TMV)has been studied as a multi-functional agent for bone tissue engineering.An osteo-inductive effect of wild-type TMV has been reported,as it can significantly enhance the bone differentiation potential of bone marrow stromal cells both on a two-dimensional substrate and in a three-dimensional(3D)hydrogel system.A TMV mutant(TMV-RGD1)was created which featured the adhesion peptide arginyl-glycyl-aspartic acid(RGD),the most common peptide motif responsible for cell adhesion to the extracellular matrix,on the surface of the virus particle to enhance the bio-functionality of the scaffold material.We hypothesised that the incorporation of either wild-type TMV or TMV-RGD1 in the 3D hydrogel scaffold would induce bone healing in critical size defects of the cranial segmental bone.We have previously tested the virus-functionalised scaffolds,in vitro,with a hyaluronic acid-based system as an in-situ hydrogel platform for 3D cell encapsulation,culture,and differentiation.The results of these experiments suggested the potential of the virus-functionalised hydrogel to promote in vitro stem cell differentiation.The hydrogel-forming system we employed was shown to be safe and biocompatible in vivo.Here,we further explored the physiological responses regarding bone regeneration of a calvarial defect in both normal and osteoporotic ovariectomized rat models.Our results,based on histological analysis in both animal models,suggested that both wild-type TMV and TMV-RGD1 functionalised hydrogels could accelerate bone regeneration,without systemic toxicity,evaluated by blood counts.New bone formation was intensified by the incorporation of the RGD-mutant viral particles.This finding increased the potential for use of the rodshaped plant virus as a platform for the addition of powerful biofunctionality for tissue engineering applications.This study was approved by the Ethics Committee on Animal Use of the Zhenjiang Affiliated First People’s Hospital affiliated to Jiangsu University.

关键词： bone regeneration calvarial defect hydrogel osteoporosis tobacco mosaic virus

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Development of personal protective equipment for the COVID-19 pandemic in Thailand and technical aspects of testing gown materials

引用

Biomaterials Translational 2021年第1期2卷 3-9页

作者： Visarut Buranasudja Anongnat Somwangthanaroj Suched Likitlersuang Tirawat Boonyatee Chartchalerm Isarankura-Na-Ayudhya jittima amie luckanagul Department of Pharmacology and Physiology Faculty of Pharmaceutical SciencesChulalongkorn UniversityBangkok Thailand Department of Chemical Engineering Faculty of EngineeringChulalongkorn UniversityBangkokThailand Centre of Excellence in Geotechnical and Geoenvironmental Engineering Department of Civil EngineeringFaculty of EngineeringChulalongkorn UniversityBangkokThailand Department of Clinical Microbiology and Applied Technology Faculty of Medical TechnologyMahidol UniversityBangkokThailand Department of Pharmaceutics and Industrial Pharmacy Faculty of Pharmaceutical SciencesChulalongkorn UniversityBangkokThailand

During the coronavirus disease 2019(COVID-19)pandemic in early 2020,Thailand,like many other countries around the world,experienced a lack of personal protective equipment(PPE)for frontline medical staff.This was not only due to the scarcity of PPE,but also because some of the available PPE did not meet the standard to properly protect frontline medical staff from the virus.During that time,the industrial sector worked closely with academic and governmental sectors to develop and produce appropriate isolation gowns,coveralls and masks.

关键词： protective properly Thailand

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

以烟草花叶病毒为半抗原载体的活体免疫反应

引用

应用化学 2015年第10期32卷 1107-1113页

作者：赵霞陈利民胡君张晓磊 jittima amie luckanagulb 林园王倩中国科学院长春应用化学研究所长春130022 南卡罗莱纳大学美国南卡罗莱纳州29208

以烟草花叶病毒的赖氨酸突变体(TMV-EPMK)为半抗原载体,利用"点击化学"反应,将半抗原小分子雌三醇(E3)经由两个不同亲/疏水性的连接臂修饰在TMV-EPMK上,成功制备了TMV-EPMK/E3完全抗原。通过免疫实验发现,以亲水性连接臂制备的TMV-EPMK... 详细信息

以烟草花叶病毒的赖氨酸突变体(TMV-EPMK)为半抗原载体,利用"点击化学"反应,将半抗原小分子雌三醇(E3)经由两个不同亲/疏水性的连接臂修饰在TMV-EPMK上,成功制备了TMV-EPMK/E3完全抗原。通过免疫实验发现,以亲水性连接臂制备的TMV-EPMK/E3偶联体可在小鼠体内诱导更高水平的E3特异性免疫反应,所产生的Ig G抗体的滴度达21800。该结果表明,以烟草花叶病毒为载体制备的完全抗原,其免疫原性受连接臂性质的影响,亲水性的连接臂更有利于抗体反应的发生。

关键词： TMV—EPMK 点击化学半抗原人工抗原免疫反应

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：