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Antioxidant activities and phytochemical constituents of Antidesma thwaitesianum Müll. Arg. leaf extracts

Journal of Integrative Medicine 2017年第4期15卷 310-319页

作者： Bhanuz Dechayont Arunporn Itharat Pathompong Phuaklee jitpisute chunthorng-orn Thana Juckmeta Nuntika Prommee Nitra Nuengchamnong Pintusorn Hansakul Department of Applied Thai Traditional Medicine Faculty of Medicine Thammasat University Center of Excellence in Applied Thai Traditional Medicine Research Thammasat University Division of Applied Thai Traditional Medicine Faculty of Public Health Naresuan University Science Laboratory Centre Faculty of Science Naresuan University Department of Preclinical Science Faculty of Medicine Thammasat University

OBJECTIVE： To investigate the antioxidant activities as well as phytochemical constituents of Antidesma thwaitesianum Mull. Arg. leaf extracts. METHODS： The leaves of A. thwaitesianum were extracted using three different methods： blending with distilled water, maceration with ethanol and decoction. The chemical antioxidant activity of the plant leaf extracts was evaluated using 2,2-diphenyl-1-picryhydrazyl （DPPH） radical and 2,2＇-azinobis（3- ethylbenzo-thiazoline-6-sulfonic acid） diammonium salt （ABTS＊） radical scavenging assays, as well as the ferric reducing antioxidant power assay. Cellular antioxidant activity was determined by superoxide and nitric oxide scavenging assays. The cytotoxicity of the leaf extracts in RAW 264.7 and differentiated HL-60 cells was tested in parallel using 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H-tetrazolium bromide and 3-（4,5-dimethylthiazol-2-yl）-5-（3-carboxymethoxyphenyl）-2-（4-sulfophenyl）-2H-tetrazolium assays, respectively. The total phenolic and flavonoid contents were also assessed by spectrophotometric analysis Phytochemical constituents of the most potent extract were investigated by liquid chromatography with an electrospray ionization quadrupole time-of-flight mass spectrometer （LC-ESI-QTOF-MS/MS）. RESULTS： The ethanolic （ME） and decoction （LW） extracts of dried leaves had the highest chemical scavenging activity against DPPH and ABTS＋ free radicals with half maximal effective concentration （EC50） values ranging from 3.54 to 6.44 μg/mL. ME and LW exerted moderate ferric reducing activity, with ferric reducing antioxidant power values of 847.41 and 941.26 mg Fe2＋/g extract, respectively. Similarly, ME showed potent cellular scavenging activity against superoxide and nitric oxide radicals with EC50 values of 58.12 and 71.90 μg/mL, respectively. However, LW exhibited only strong nitric oxide scavenging activity with an EC50 value of 91.20 μg/mL. The cell viability of RAW 264.7 and HL-60 cells was greater than 70% in all tes

关键词： Antidesma thwaitesianum Mull. Arg. antioxidants phytochemicals spectrometry, mass,electrospray ionization chromatography, liquid

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