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身体质量指数对桡骨远端骨折严重程度和手术时间的影响

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中华手外科杂志 2024年第1期40卷 57-62页

作者：叶曙明金能李晶张积森谢武昆荆珏华安徽医科大学第二附属医院骨科合肥230601 安徽医科大学公共卫生学院儿少卫生与妇幼保健学系合肥230601

目的观察身体质量指数(body mass index,BMI)对桡骨远端骨折严重程度和手术时间的影响。方法我科自2016年6月至2022年7月收治桡骨远端骨折患者84例,依照桡骨远端骨折的AO分型进行分组:A组29例(23-A2.2、23-A2.3、23-A3型),B组26例(23-B... 详细信息

目的观察身体质量指数(body mass index,BMI)对桡骨远端骨折严重程度和手术时间的影响。方法我科自2016年6月至2022年7月收治桡骨远端骨折患者84例,依照桡骨远端骨折的AO分型进行分组:A组29例(23-A2.2、23-A2.3、23-A3型),B组26例(23-B型),C组29例(23-C型)。记录患者的年龄,性别,吸烟史,受伤至医院时间,是否伴有糖尿病、高血压病史,受伤的侧别,手术时间及BMI。利用卡方检验、Logistic回归及线性回归分析的方法评估年龄、性别、吸烟史、受伤的侧别、受伤至医院时间、是否伴有糖尿病高血压病史、BMI与A,B,C三组之间的关系。使用线性回归模型分析BMI对桡骨远端骨折手术时间的影响,均以P<0.05为差异具有统计学意义。结果84例患者获得完整随访,使用卡方检验比较A、B、C三组的年龄、性别、吸烟史、受伤至医院时间、是否伴有糖尿病高血压病史、BMI和受伤侧别之间的差异,发现年龄、性别、吸烟史和BMI在组间差异有统计学意义(P<0.05)。对年龄、性别、吸烟史、BMI、受伤侧别、受伤至医院时间、是否伴有糖尿病高血压病史进行多因素Logistic回归分析,筛选出年龄和BMI是致桡骨远端骨折的危险因素(P<0.05)。把A组作为对照组进一步分析BMI对B组和C组的影响,得出BMI是发生23-B型和23-C型桡骨远端骨折的危险因素。使用线性回归分析BMI对桡骨远端骨折手术时间的影响,得出BMI与手术时间有显著相关性(P<0.01)。结论BMI是发生23-B型和23-C型桡骨远端骨折的危险因素,并且BMI能影响桡骨远端骨折患者的手术时间,随着BMI的增高,手术时间相应延长。

关键词：桡骨骨折身体质量指数手术时间统计分析

A growth-regulating factor 7 (GRF7)-mediated gene regulatory network promotes leaf growth and expansion in sugarcane

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甘蔗抗褐锈病Bru1区域的鉴定及候选抗病基因的功能分析

The Crop Journal 2024年第2期 422页

作者： Qiaoyu Wang Yihan Li Dadong Lin Xiaoxi Feng Yongjun Wang Tianyou Wang Hongyan Ding jisen zhang

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中国农业科学 2024年第5期57卷 855-867页

作者：吴期滨谢婉婕钟惠冯春燕潘浩然齐浥颖张积森王恒波福建农林大学农学院/国家甘蔗工程技术研究中心/农业农村部福建甘蔗生物学与遗传育种重点实验室广西大学亚热带农业生物资源保护与利用国家重点实验室

【目的】甘蔗褐锈病是由黑顶柄锈菌（Puccinia melanocephala H. Sydow&P. Sydow）引起的最具破坏性的真菌病害之一，能造成蔗糖分降低10%—36%，严重威胁甘蔗产业发展。已有研究揭示抗褐锈病主效基因定位于Bru1区域，克隆其关键候选基... 详细信息

【目的】甘蔗褐锈病是由黑顶柄锈菌（Puccinia melanocephala H. Sydow&P. Sydow）引起的最具破坏性的真菌病害之一，能造成蔗糖分降低10%—36%，严重威胁甘蔗产业发展。已有研究揭示抗褐锈病主效基因定位于Bru1区域，克隆其关键候选基因，并进行功能研究，为选育抗褐锈病甘蔗新品种提供重要的候选基因资源。【方法】利用PacBio SequelⅡ测序平台获得甘蔗栽培品种ROC22的contig水平基因组信息，鉴定到抗褐锈病Bru1区域，对其进行基因注释和克隆，分析其组织特异性和抗、感褐锈病甘蔗品种中的表达模式及其在烟草叶片的亚细胞定位和瞬时表达。【结果】基于Bru1位点区域紧密连锁的标记R12H16和9O20-F4，从该区域中注释到33个基因，结合典型抗病基因结构域，共筛选获得5个候选抗病基因，分别为Brrg99、Brrg103、Brrg108、Brrg115和Brrg116。以甘蔗栽培品种ROC22的cDNA为模板，克隆获得Brrg116的全长序列729 bp，编码242个氨基酸残基。将该基因序列分别比对甘蔗热带种和割手密种及二倍体近缘种高粱的基因组数据库，发现其与热带种和割手密种的同源基因序列相似性很高，达到98%以上，而与高粱的相似性为93.77%。系统进化树揭示该基因起源甘蔗割手密种。实时荧光定量PCR检测表明，Brrg116在甘蔗栽培品种的多个组织中组成型表达，尤其是在+1叶中的表达量最高，是蔗芽的9.8倍；在褐锈病菌侵染抗、感甘蔗栽培品种的6和72 h时，呈现显著差异表达。亚细胞定位结果显示，该基因编码蛋白定位于细胞膜上。在本氏烟（Nicotiana benthamiana）叶片中瞬时过表达Brrg116，其后接种茄病镰刀菌蓝色变种，二氨基联苯胺法（3,3′-diaminobenzidine,DAB）染色逐渐加深，超敏反应相关基因、水杨酸信号和乙烯信号途径中的相关基因持续上调表达。【结论】Brrg116在甘蔗不同组织中组成型表达，且在受褐锈病菌侵染的抗病甘蔗栽培品种中呈现快速的诱导表达。此外，过表达Brrg116引发水杨酸和乙烯等激素信号通路产生防御响应，推测该基因可能在提高植物的抗病性方面发挥重要调控作用。

关键词：甘蔗 Bru1区域褐锈病候选抗病基因连锁标记

Species-specific regulatory pathways of small RNAs play sophisticated roles in flower development in Dimocarpus longan Lour.

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Horticultural Plant Journal 2023年第2期9卷 237-249页

作者： Bo Liu Guanliang Li Chengjie Chen Zaohai Zeng Jing Xu jisen zhang Rui Xia Yuanlong Liu State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural UniversityGuangzhouGuangdong 510640China Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural UniversityGuangzhouGuangdong 510640China Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in South China Ministry of Agriculture and Rural AffairsSouth China Agricultural UniversityGuangzhouGuangdong 510640China Center for Genomics and Biotechnology Haixia Institute of Science and TechnologyCollege of Life SciencesFujian Agriculture and Forestry UniversityFuzhouFujian 350002China

Flower development plays vital role in horticultural ***-transcriptional regulation via small RNAs is important for plant flower *** uncover post-transcriptional regulatory networks during the flower development in Dimocarpus longan Lour.‘Shixia’,an economically important fruit crop in subtropical regions,we collected and analyzed sRNA deep-sequencing datasets and degradome libraries Apart from identifying miRNAs and phased siRNA generating loci(PHAS loci),120 hairpin loci,producing abundant sRNAs,were identified by in-house *** results suggested that 56 miRNA-target pairs,2221-nt-PHAS loci,and 111 hairpin loci are involved in posttranscriptional gene silencing during longan reproductive ***-specific or species-specific post-transcriptional regulatory modules have been unveiled,including miR482-PHAS and ***482-PHAS might be involved in longan flower development beyond their conserved roles in plant defense,and miRN15 is a novel miRNA likely associated with a hairpin locus(HPL-056)to regulate strigolactone receptor gene DWARF14(D14)and the biogenesis of phasiRNAs from *** small RNAs are enriched in flower buds,suggesting they are likely involved in post-transcriptional regulatory networks essential for longan flower development via the strigolactone signaling pathway.

关键词： Dimocarpus longan Lour Small RNAs Flower development Post-transcriptional regulation

Detection of SARS-CoV-2 based on artificial intelligence-assisted smartphone:A review

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Chinese Chemical Letters 2024年第7期35卷 25-34页

作者： Yunxin Li Jinghui zhang jisen Chen Feng Zhu Zhiqiang Liu Peng Bao Wei Shen Sheng Tang School of Environmental and Chemical Engineering Jiangsu University of Science and TechnologyZhenjiang 212003China Programme in Emerging Infectious Diseases Duke-NUS Medical SchoolSingapore 169857Singapore

In recent years,the application of smartphone in various fields has received great attention,and it has become a promising tool in virus detection,data processing and data *** the rapid spread of COVID-19 around the world,many traditional detection methods have been combined with smartphone to assist in the analysis and detection of the novel coronavirus(SARS-CoV-2),including electrochemistry,fluorescence and *** the gradual development of artificial intelligence(AI),the combination of AI and smartphone to analyze SARS-CoV-2 was also the focus of *** on the summary of the traditional methods combined with smartphone to detect SARS-CoV-2 virus,in addition to AI-based data processing,AI algorithms are also employed for SARS-CoV-2 detection *** review discussed both strategies and focused on the application of the *** combination of AI algorithm and smartphone to detect SARS-CoV-2 has high accuracy,which is more conducive to meeting the needs of portable *** addition,the classification of SARS-CoV-2 virus samples in biological fluids such as blood and saliva was also ***,this paper briefly discussed the limitations of using smartphone analysis to detect SARS-CoV-2,as well as the prospect and future development of virus *** conclusion,the detection methods based on smartphone and AI algorithms show great potential in the detection of SARS-CoV-2 and can be a valuable complement to traditional analysis methods.

关键词： COVID-19 SARS-CoV-2 virus Detection method Smartphone analysis Artificial intelligence

Shape-Controlled Synthesis and Upconversion Luminescence of Y2O3: Yb, Er Microstructures

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Journal of Rare Earths 2004年第Z1期22卷 140-143页

作者： De Gejihu Qin Weiping zhang jisen Zhao Dan Key Laboratory of Excited State Processes Changchun Institute of Optics Fine Mechanics and Physics Chinese Academy of Science Changchun 130033 China Key Laboratory of Excited State Processes Changchun Institute of Optics Fine Mechanics and Physics Chinese Academy of Science Changchun 130033 China Key Laboratory of Excited State Processes Changchun Institute of Optics Fine Mechanics and Physics Chinese Academy of Science Changchun 130033 China Key Laboratory of Excited State Processes Changchun Institute of Optics Fine Mechanics and Physics Chinese Academy of Science Changchun 130033 China

The Y2O3: Yb3+ , Er3 + microstructures were fabricated by a hydrothermal method without *** microstructures structure was characterized by X-ray diffraction (XRD) and scanning electron microscope (SEM,KYKY 1000B).The up-conversion luminescence spectra were studied under 978 nm laser diode *** Yb3 + and Er3 + codoped Y2 O3 microcrystals, the relative intensity of green emission became stronger as the morphology of sample changed from wires to films.

关键词： upconversion luminescence Y2O3: Yb3 + , Er3 + microstructures rare earths

亚热带特色农业的本科教改探索——以广西大学农学院为例

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基因组学与应用生物学 2023年第11期42卷 1261-1266页

作者：张积森徐益广西大学农学院南宁530004 广西大学生命科学与技术学院南宁530004

我国亚热带地区作为重要的农业生产区域之一,具有独特的气候、土壤和植被条件,孕育了鲜明的农业特色。广西是我国最大的亚热带农业省(区),但在广西高校的农学本科教育中,对亚热带农业特色的关注度稍显不足。以广西大学农学院为例,主要... 详细信息

我国亚热带地区作为重要的农业生产区域之一,具有独特的气候、土壤和植被条件,孕育了鲜明的农业特色。广西是我国最大的亚热带农业省(区),但在广西高校的农学本科教育中,对亚热带农业特色的关注度稍显不足。以广西大学农学院为例,主要表现在课程设置不够完善、学生缺乏实践机会和师资力量不足三方面,导致学生在相关领域的专业素养和实践能力有限。在实施“双碳”(即碳达峰和碳中和)目标的时代背景下,随着人工智能、现代生物育种技术等新兴技术的崛起,传统农业正在向智慧农业转变,农学本科教学面临着新农科建设的挑战和机遇。因此,亚热带特色农业本科教学需要进一步加强亚热带农业特色课程的建设、扩大师资队伍、加强学生交流合作平台建设,并与农业生产实际紧密结合。

关键词：亚热带农业本科教改智慧农业乡村振兴

甘蔗基因工程的研究进展:探索转化和CRISPR技术的前沿突破

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基因组学与应用生物学 2023年第12期42卷 1275-1282页

作者：孟雪刘刚张积森福建农林大学生命科学学院福州350002 福建农林大学未来技术学院福州350002 广西大学农学院亚热带农业生物资源保护与利用国家重点实验室南宁530004

甘蔗(Saccharum officinarum)是世界上最重要的经济作物之一,作为原料生产了全世界80%以上的食糖和40%左右的酒精。传统育种方法在改良甘蔗品种方面效率太低,而转基因和CRISPR等分子技术为甘蔗改良提供新途径。本文总结了甘蔗基因工程进... 详细信息

甘蔗(Saccharum officinarum)是世界上最重要的经济作物之一,作为原料生产了全世界80%以上的食糖和40%左右的酒精。传统育种方法在改良甘蔗品种方面效率太低,而转基因和CRISPR等分子技术为甘蔗改良提供新途径。本文总结了甘蔗基因工程进展,包括提高甘蔗的遗传转化效率和提高CRISPR编辑精确性,强调了改进基因技术对甘蔗品种改良的关键性,该技术可为未来农业和食品工业带来希望。

关键词：甘蔗基因工程 CRISPR 基因编辑

A near-complete genome assembly of the allotetrapolyploid Cenchrus fungigraminus(JUJUNCAO)provides insights into its evolution and C4 photosynthesis

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Plant Communications 2023年第5期4卷 324-341页

作者： Huakun Zheng Baiyu Wang Xiuting Hua Ruiting Gao Yuhao Wang Zixin zhang Yixing zhang Jing Mei Yongji Huang Yumin Huang Hui Lin Xingtan zhang Dongmei Lin Siren Lan Zhongjian Liu Guodong Lu Zonghua Wang Ray Ming jisen zhang Zhanxi Lin National Engineering Research Center of JUNCAO Technology College of Life ScienceFujian Agriculture and Forestry UniversityFuzhou 350002China State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources Guangxi Key Laboratory of Sugarcane BiologyGuangxi UniversityNanning 530004GuangxiChina Center for Genomics Fujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyKey Laboratory of GeneticsBreeding and Multiple Utilization of CropsMinistry of EducationFujian Agriculture and Forestry UniversityFuzhou 350002China Fuzhou Institute of Oceanography Minjiang UniversityFuzhou 350108China

JUJUNCAO(Cenchrus fungigraminus;2n=4x=28)is a Cenchrus grass with the highest biomass production among cultivated plants,and it can be used for mushroom cultivation,animal feed,and biofuel ***,we report a nearly complete genome assembly of JUJUNCAO and reveal that JUJUNCAO is an allopolyploid that originated2.7 million years ago(mya).Its genome consists of two subgenomes,and subgenome A shares high collinear synteny with pearl *** also investigated the genome evolution of JUJUNCAO and suggest that the ancestral karyotype of Cenchrus split into the A and B ancestral karyotypes of *** transcriptome and DNA methylome analyses revealed functional divergence of homeologous gene pairs between the two subgenomes,which was a further indication of asymmetric DNA *** three types of centromeric repeat in the JUJUNCAO genome(CEN137,CEN148,and CEN156)may have evolved independently within each subgenome,with some introgressions of CEN156 from the B to the A *** investigated the photosynthetic characteristics of JUJUNCAO,revealing its typical C4 Kranz anatomy and high photosynthetic ***-ME and PEPCK appear to cooperate in the major C4 decarboxylation reaction of JUJUNCAO,which is different from other C4 photosynthetic subtypes and may contribute to its high photosynthetic efficiency and biomass *** together,our results provide insights into the highly efficient photosynthetic mechanism of JUJUNCAO and provide a valuable reference genome for future genetic and evolutionary studies,as well as genetic improvement of Cenchrus grasses.

关键词： genome assembly allotetrapolyploid centromere architecture evolutionary trajectory photosynthetic efficiency Cenchrus grass