检索结果-南通市图书馆

Expanding the Coverage of Metabolic Landscape in Cultivated Rice with Integrated Computational Approaches

Genomics, Proteomics & Bioinformatics 2022年第4期20卷 702-714页

作者： Xuetong Li Hongxia Zhou Ning Xiao Xueting Wu Yuanhong Shan Longxian Chen Cuiting Wang Zixuan Wang jirong huang Aihong Li Xuan Li CAS Key Laboratory of Synthetic Biology/National Center for Gene Research CAS Center for Excellence in Molecular Plant Sciences/Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China Lixiahe Agricultural Research Institute of Jiangsu Province Yangzhou 225007China Shanghai Key Laboratory of Plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 200234China University of Chinese Academy of Sciences Beijing 100049China

Genome-scale metabolomics analysis is increasingly used for pathway and function discovery in the post-genomics era.The great potential offered by developed mass spectrometry(MS)-based technologies has been hindered,since only a small portion of detected metabolites were identifiable so far.To address the critical issue of low identification coverage in metabolomics,we adopted a deep metabolomics analysis strategy by integrating advanced algorithms and expanded reference databases.The experimental reference spectra and in silico reference spectra were adopted to facilitate the structural annotation.To further characterize the structure of metabolites,two approaches were incorporated into our strategy,i.e.,structural motif search combined with neutral loss scanning and metabolite association network.Untargeted metabolomics analysis was performed on 150 rice cultivars using ultra-performance liquid chromatography coupled with quadrupoleOrbitrap MS.Consequently,a total of 1939 out of 4491 metabolite features in the MS/MS spectral tag(MS2T)library were annotated,representing an extension of annotation coverage by an order of magnitude in rice.The differential accumulation patterns of flavonoids between indica and japonica cultivars were revealed,especially O-sulfated flavonoids.A series of closely-related flavonolignans were characterized,adding further evidence for the crucial role of tricin-oligolignols in lignification.Our study provides an important protocol for exploring phytochemical diversity in other plant species.

关键词： Untargeted metabolomics MS/MS spectral tag Structural characterization Phytochemical diversity Flavonoid derivative

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Lift the mystery veil on chlorophyllases

引用

Molecular Plant 2021年第7期14卷 1044-1045页

作者： Weihua huang jirong huang Shanghai Key Laboratory of Plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 200234China

PhotosystemⅡ(PSⅡ)is a large membrane-protein complex that catalyzes the splitting of water into protons,electrons,and oxygen via harvesting light energy(Johnson,2016).The reaction center of PSⅡ,operating like an electron pump,is a special pair of chlorophyll a(Chi a),referred to as P680,bound to D1 and D2 subunits,each with its set of electron carriers.Upon absorption of light energy,P680 is excited and donates an electron to the neighboring pheophytin(Pheo),producing a pair of radicals(·P680+and·Pheo-),called charge separation.

关键词： neighboring excited splitting

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Arabidopsis BIG1 and BIG5 are crucial for male gametophyte transmission

引用

Journal of Integrative Plant Biology 2019年第9期61卷 981-986页

作者： Yiping Suo jirong huang Department of Biology College of Life and Environmental Sciences Shanghai Normal University

Arabidopsis contains five Brefeldin Ainhibited guanine nucleotide exchange factors(BIGs),which play a critical role in vesicle biogenesis for protein traffic from the Golgi to the plasma membrane.Biological processes regulated by BIG1-BIG4 are postulated to be distinct from those by BIG5. However, we show that the self-pollinated BIG1+/- big5 silique do not produce homozygous seeds, and some pollen tubes from BIG1+/- big5 anthers grew slowly in vitro and failed to target nearby ovules in vivo. We identified the big1 big5 homozygote from the progeny of BIG1+/- big5 plants transformed with BIG5, whose expression is driven by a pollen-specific promoter p Lat52, indicating that male gametophyte transmission is blocked in the double mutant. Confocal microscopy indicated that BIG1 and BIG5 are co-localized in trans Golgi network. Thus,our data indicate that BIG1 and BIG5 are crucial for male gametophyte transmission.

关键词： Arabidopsis BIG1 and BIG5 male gametophyte transmission

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A Crucial Role of the RGS Domain in Trans- Golgi Network Export of AtRGS1 in the Protein Secretory Pathway

引用

Molecular Plant 2013年第6期6卷 1933-1944页

作者： Guangzhen Hu Yiping Suo jirong huang National Key Laboratory of Plant Molecular Genetics Shanghai Institute of Plant Physiology and Ecology Shanghai Institutes for Biological SciencesChinese Academy of Sciences 300 Feng Lin Road. Shanghai 200032 China Present address: Division of Hematology Department of Internal Medicine Mayo Clinic Rochester MN 55905 USA

The secretory pathway is responsible for the transport of newly synthesized transmembrane proteins from the endoplasmic reticulum to their destinations via the Golgi/trans-Golgi network （TGN）, Cargo proteins at each sta- tion are actively sorted by specific sorting signals on the cargo and the corresponding coat complexes. Here, we used the Arabidopsis regulator of G-protein signaling （AtRGS1）, which contains an N-terminal potentially sensing glucose seven-transmembrane domain and a C-terminal RGS domain, as a model to uncover sorting motifs required for its cell surface expression. Expression of wild-type and truncated or mutated AtRGS1 fluorescent fusion proteins identified two cysteine residues in the extracellular N-terminus that are essential for endoplasmic reticulum exit and/or correct folding of AtRGS1. The linker between the seven-transmembrane and RGS domains contains an endoplasmic reticulum export signal, whereas the C-terminus is dispensable for the plasma membrane expression of AtRGS1. Interestingly, deletion of the RGS domain results in Golgi/TGN localization of the truncated AtRGS1. Further analysis using site-directed mutagen- esis showed that a tyrosine-based motif embedded in the RGS domain is essential for Golgi/TGN export of AtRGS1. These results reveal a new role for the RGS domain in regulating AtRGS1 trafficking from the Golgi/TGN to the plasma membrane and explain the interaction between the seven-transmembrane and RGS domains.

关键词： G-proteins AtRGS1 TGN sorting signal RGS domain Arabidopsis.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

DELLA Proteins Promote Anthocyanin Biosynthesis via Sequestering MYBL2 and JAZ Suppressors of the MYB/bHLH/WD40 Complex in Arabidopsis thaliana

引用

Molecular Plant 2016年第5期9卷 711-721页

作者： Ye Xie Huijuan Tan Zhaoxue Ma jirong huang National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant Sciences Institute of Plant Physiology and EcologyShanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China University of the Chinese Academy of Sciences Beijing 100049 China

Anthocyanin accumulation is recognized as a visible biomarker of plants that have suffered from environmental stresses. However, the molecular mechanisms underlying stress-induced anthocyanin biosynthesis remain unclear. Expression of anthocyanin-specific genes is regulated by the conserved MBW complex, which is composed of the MYB, bHLH, and WD40 subunRs in higher plants. MBW activity is repressed by MYBL2 and the JAZ family proteins, which bind competitively to bHLH and MYB/bHLH, respectively. Here, we found that MYBL2 and JAZs mediate gibberellic acid-inhibRed anthocyanin biosynthesis in Arabidopsis. Competitive pull-down and dual-lucifarase assays showed that DELLA proteins directly sequester MYBL2 and JAZ repressors, leading to the release of bHLH/MYB subunits and subsequently to the formation of active MBW complex, which then activates the anthocyanin biosynthetic pathway. The JAZ-DELLA-MYBL2 module also plays an Important role in abiotic stress-induced anthocy- anin biosynthesis. Furthermore, we found that the DELLA protein RGA accumulates upon plant exposure to abiotic stresses. Altogether, our data reveal that DELLA-promoted anthocyanin biosynthesis is mediated at least in part by MYBL2 and JAZ regulatory proteins, providing new insights into the coordinated regulation of plant growth and defense through metabolic pathway regulation.

关键词： DELLA MYBL2 JAZ anthocyanin biosynthesis abiotic stresses

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Arabidopsis pollen-specific glycerophosphodiester phosphodiesterase-like genes are essential for pollen tube tip growth

引用

Journal of Integrative Plant Biology 2023年第8期65卷 2001-2017页

作者： Chong Wang Hao Cheng Wenjing Xu Jingshi Xue Xinguo Hua Guimin Tong Xujun Ma Chuanping Yang Xingguo Lan Shi-Yi Shen Zhongnan Yang jirong huang Yuxiang Cheng State Key Laboratory of Tree Genetics and Breeding Northeast Forestry UniversityHarbin 150040China Shanghai Key Laboratory of Plant Molecular Sciences Shanghai Normal UniversityShanghai 200234China College of Life Sciences Northeast Forestry UniversityHarbin 150040China

In angiosperms,pollen tube growth is critical for double fertilization and seed formation.Many of the factors involved in pollen tube tip growth are unknown.Here,we report the roles of pollenspecific GLYCEROPHOSPHODIESTER PHOSPHO DIESTERASE-LIKE(GDPD-LIKE)genes in pollen tube tip growth.Arabidopsis thaliana GDPD-LIKE6(At GDPDL6)and At GDPDL7 were specifically expressed in mature pollen grains and pollen tubes and green fluorescent protein(GFP)-At GDPDL6 and GFP-At GDPDL7 fusion proteins were enriched at the plasma membrane at the apex of forming pollen tubes.Atgdpdl6 Atgdpdl7 double mutants displayed severe sterility that was rescued by genetic complementation with At GDPDL6 or At GDPDL7.This sterility was associated with defective male gametophytic transmission.Atgdpdl6 Atgdpdl7 pollen tubes burst immediately after initiation of pollen germination in vitro and in vivo,consistent with the thin and fragile walls in their tips.Cellulose deposition was greatly reduced along the mutant pollen tube tip walls,and the localization of pollen-specific CELLULOSE SYNTHASE-LIKE D1(CSLD1)and CSLD4 was impaired to the apex of mutant pollen tubes.A rice pollen-specific GDPD-LIKE protein also contributed to pollen tube tip growth,suggesting that members of this family have conserved functions in angiosperms.Thus,pollen-specific GDPDLIKEs mediate pollen tube tip growth,possibly by modulating cellulose deposition in pollen tube walls.

关键词： Arabidopsis thaliana cell wall integrity cellulose deposition male sterility pollen-specific GDPD-LIKE pollen tube tip growth

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

An ubiquitin-like protein SDE2 negatively affects sucrose-induced anthocyanin biosynthesis in Arabidopsis

引用

Science Bulletin 2017年第23期62卷 1585-1592页

作者： Ye Xie Yulei Zhao Huijuan Tan Qin Chen jirong huang National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant Sciences Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China University of the Chinese Academy of Sciences Beijing 100049 China School of Life Sciences Shanghai University Shanghai 200444 China College of Life and Environmental Sciences Shanghai Normal University Shanghai 200234 China

Anthocyanin biosynthesis is regulated by a conserved transcriptional MBW complex composed of MYB,b HLH and WD40 subunits. However, molecular mechanisms underlying transcriptional regulation of these MBW subunits remain largely elusive. In this study, we isolated an Arabidopsis mutant that displays a constitutive red color in aboveground tissues with retarded growth phenotypes. In the presence of sucrose, the mutant accumulates more than 3-fold anthocyanins of the wild type(WT), but cannot produce anthocyanins as WT in the absence of sucrose. Map-based cloning results demonstrated that the mutation occurs in the locus At4 G01000, which encodes a conserved nuclear-localized ubiquitin-like(UBL) superfamily protein, silencing defective 2(SDE2), in eukaryotes. SDE2 is ubiquitously expressed in various tissues. In the sucrose-induced anthocyanin biosynthesis, SDE2 expression was not responded to sucrose treatment at the early stage but was enhanced at the late stage. SDE2 mutations result in upregulation of anthocyanin biosynthetic and regulatory genes. Yeast-two hybrid analysis indicated that SDE2 has no direct interaction with the MYB transcription factor PAP1 and b HLH factor TT8, indicating that SDE2 is a indirect factor to affect anthocyanin accumulation. Taking together, our data suggest that SDE2 may play a role in finely coordinating anthocyanin biosynthesis with other biological processes.

关键词： Anthocyanin biosynthesis Sucrose SDE2 PAP1 Arabidopsis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Defective Etioplasts Observed in Variegation Mutants May Reveal the Light-Independent Regulation of White/Yellow Sectors of Arabidopsis Leaves

引用

Journal of Integrative Plant Biology 2011年第11期53卷 846-857页

作者： Wenjuan Wu Nabil Elsheery Qing Wei Lingang Zhang jirong huang National Key Laboratory of Plant Molecular Genetics Institute of Plant-PhysTology and-Ecology Shanghai Institutesfor Biological Sciences the Chinese Academy of Sciences Shanghai 200032 China present address： Division of Nephrology and Hypertension Mayo Clinic Rochester MN 55905 USA Present address： Institute of Plant Science and Resources okayama University Kurashiki Okayama Japan

Leaf variegation resulting from nuclear gene mutations has been used as a model system to elucidate the molecular mechanisms of chloroplast development. Since most variegation genes also function in photosynthesis, it remains unknown whether their roles in photosynthesis and chloroplast development are distinct. Here, using the variegation mutant thylakoid formation1 （thfl） we show that variegation formation is light independent. It was found that slow and uneven chloroplast development in thfl can be attributed to defects in etioplast development in darkness. Ultrastructural analysis showed the coexistence of plastids with or without prolamellar bodies （PLB） in cells of thfl, but not of WT. Although THF1 mutation leads to significant decreases in the levels of Pchlide and Pchliide oxidoreductase （POR） expression, genetic and 5-aminolevulinic acid （ALA）-feeding analysis did not reveal Pchlide or POR to be critical factors for etioplast formation in thfl. Northern blot analysis showed that plastid gene expression is dramatically reduced in thfl compared with that in WT, particularly in the dark. Our results also indicate that chlorophyll biosynthesis and expression of plastidic genes are coordinately suppressed in thfl. Based on these results, we propose a model to explain leaf variegation formation from the plastid development perspective.

关键词： Arabidopsis chloroplast etioplast THF1/Psb29 variegation Pchllide oxidoreductase Pchlide oxidoreductase chloroplastdevelopment.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A Crucial Role of GA-Regulated Flavonol Biosynthesis in Root Growth of Arabidopsis

引用

Molecular Plant 2019年第4期12卷 521-537页

作者： Huijuan Tan Cong Man Ye Xie Jijun Yan Jinfang Chu jirong huang National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant Sciences Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China University of the Chinese Academy of Sciences Beijing 100049 China National Center for Plant Gene Research (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Shanghai Key Laboratory of Plant Molecular Sciences College of Life Sciences Shanghai Normal University Shanghai 200234 China

Flavonols have been demonstrated to play many important roles in plant growth, development, and communication with other organisms. Flavonol biosynthesis is spatiotemporally regulated by the subgroup 7 R2R3-MYB (SG7 MYB) transcription factors including MYB11/MYB12/MYB111. However, whether SG7- MYB activity is subject to post-translational regulation remains unclear. Here, we show that gibberellic acid (GA) inhibits flavonol biosynthesis via DELLA proteins in Arabidopsis. Protein-protein interaction analyses revealed that DELLAs (RGA and GAI) interacted with SG7 MYBs (MYB12 and MYB111) both in vitro and in vivo, leading to enhanced affinity of MYB binding to the promoter regions of key genes for flavonol biosynthesis and thus increasing their transcriptional levels. We observed that the level of auxin in the root tip was negatively correlated with root flavonol content. Furthermore, genetic assays showed that loss-of-function mutations in MYB 12, which is predominantly expressed in roots, partially rescued the short-root phenotype of the GA-deficient mutant ga1-3 by increasing root meristem size and mature cell size. Consistent with these observations, exogenous application of the flavonol quercetin restored the root meristem size of myb12 ga1-3 to that of ga1-3. Taken together, our data elucidate a molecular mechanism by which GA promotes root growth by directly reducing flavonol biosynthesis.

关键词： gibberellin flavonol root growth auxin stress

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Arogenate Dehydratase Isoforms Differentially Regulate Anthocyanin Biosynthesis in Arabidopsis thaliana

引用

Molecular Plant 2016年第12期9卷 1609-1619页

作者： Qingbo Chen Cong Man Danning Li Huijuan Tan Ye Xie jirong huang National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant Sciences Shanghai Institute of Plant Physiology and Ecology Chinese Academy of Sciences 300 Fenglin Road Shanghai 200032 China These authors contributed equally to this article

Anthocyanins, a group of L-phenylalanine （Phe）-derived flavonoids, have been demonstrated to play impor- tant roles in plant stress resistance and interactions between plants and insects. Although the anthocyanin biosynthetic pathway and its regulatory mechanisms have been extensively studied, it remains unclear whether the level of Phe supply affects anthocyanin biosynthesis. Here, we investigated the roles of arogenate dehydratases （ADTs）, the key enzymes that catalyze the conversion of arogenate into Phe, in sucrose-induced anthocyanin biosynthesis in Arabidopsis. Genetic analysis showed that all six ADT isoforms function redundantly in anthocyanin biosynthesis but have differential contributions. ADT2 contributes the most to anthocyanin accumulation, followed by ADT1 and ADT3, and ADT4-ADT6. We found that anthocyanin content is positively correlated with the levels of Phe and sucrose-induced ADT transcripts in seedlings. Consistently, addition of Phe to the medium could dramatically increase anthocyanin content in the wild-type plants and rescue the phenotype of the adtl adt3 double mutant regarding the anthocyanin accumulation. Moreover, transgenic plants overexpressing ADT4, which appears to be less sensitive to Phe than overexpression ofADT2, hyperaccumulate Phe and produce elevated level of antho- cyanins. Taken together, our results suggest that the level of Phe is an important regulatory factor for sus- taining anthocyanin biosynthesis.

关键词： phenylalanine metabolic flux ADT anthocyanin Arabidopsis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：