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Seamless integration of above-and undercanopy unmanned aerial vehicle laser scanning for forest investigation

Forest Ecosystems 2021年第1期8卷 124-138页

作者： Yunsheng Wang Antero Kukko Eric Hyyppä Teemu Hakala jiri Pyörälä Matti Lehtomäki Aimad El Issaoui Xiaowei Yu Harri Kaartinen Xinlian Liang Juha Hyyppä Department of Remote Sensing and Photogrammetry Finnish Geospatial Research Institute FGIThe National Land Survey of Finland02431 MasalaFinland Department of Forest Sciences University of HelsinkiFI-00014 HelsinkiFinland Department of Geography and Geology University of TurkuFI-20500 TurkuFinland

Background:Current automated forest investigation is facing a dilemma over how to achieve high tree-and plotlevel completeness while maintaining a high cost and labor *** study tackles the challenge by exploring a new concept that enables an efficient fusion of aerial and terrestrial perspectives for digitizing and characterizing individual trees in forests through an Unmanned Aerial Vehicle(UAV)that flies above and under canopies in a single *** advantage of such concept is that the aerial perspective from the above-canopy UAV and the terrestrial perspective from the under-canopy UAV can be seamlessly integrated in one flight,thus grants the access to simultaneous high completeness,high efficiency,and low ***:In the experiment,an approximately 0.5 ha forest was covered in ca.10 min from takeoff to *** GNSS-IMU based positioning supports a geometric accuracy of the produced point cloud that is equivalent to that of the mobile mapping systems,which leads to a 2–4 cm RMSE of the diameter at the breast height estimates,and a 4–7 cm RMSE of the stem curve ***:Results of the experiment suggested that the integrated flight is capable of combining the high completeness of upper canopies from the above-canopy perspective and the high completeness of stems from the terrestrial ***,it is a solution to combine the advantages of the terrestrial static,the mobile,and the above-canopy UAV observations,which is a promising step forward to achieve a fully autonomous in situ forest *** studies should be aimed to further improve the platform positioning,and to automatize the UAV operation.

关键词： Forest In situ Inventory Above canopy Under canopy Unmanned aerial vehicle Laser scanning Point cloud Close range remote sensing

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Helicobacter pylori infection and other bacteria in pancreatic cancer and autoimmune pancreatitis

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World Journal of Gastrointestinal Oncology 2021年第8期13卷 835-844页

作者： Lumir Kunovsky Petr Dite Petr Jabandziev jiri Dolina Jitka Vaculova Martin Blaho Martina Bojkova Jana Dvorackova Magdalena Uvirova Zdenek Kala Jan Trna Department of Surgery University Hospital Brno and Faculty of MedicineMasaryk UniversityBrno 62500Czech Republic Department of Gastroenterology and Internal Medicine University Hospital Brno and Faculty of MedicineMasaryk UniversityBrno 62500Czech Republic Department of Gastroenterology and Internal Medicine University Hospital OstravaOstrava 70800Czech Republic Faculty of Medicine University of OstravaOstrava 70300Czech Republic Department of Pediatrics University Hospital Brno and Faculty of MedicineMasaryk UniversityBrno 61300Czech Republic Central European Institute of Technology Masaryk UniversityBrno 62500Czech Republic Department of Intensive Medicine Emergency Medicine and Forensic StudiesUniversity Hospital OstravaOstrava 70800Czech Republic CGB Laboratory a.s.Ostrava Ostrava 70300Czech Republic Department of Gastroenterology and Digestive Endoscopy Masaryk Memorial Cancer InstituteBrno 65653Czech Republic Department of Internal Medicine Hospital BoskoviceBoskovice 68001Czech Republic

Helicobacter pylori(***)is an infectious agent influencing as much as 50%of the world’s *** is the causative agent for several diseases,most especially gastric and duodenal peptic ulcer,gastric adenocarcinoma and mucosaassociated lymphoid tissue lymphoma of the stomach.A number of other,extragastric manifestations also are associated with *** *** include neurological disorders,such as Alzheimer’s disease,demyelinating multiple sclerosis and Parkinson’s *** is also evidence for a relationship between *** infection and such dermatological diseases as psoriasis and rosacea as well as a connection with infection and open-angle *** little is known about the relationship between *** infection and diseases of the *** evidence about *** and its potential role in the development of pancreatic diseases concerns pancreatic adenocarcinoma and autoimmune forms of chronic *** is data(albeit not fully consistent)indicating modestly increased pancreatic cancer risk in ***-positive *** pathogenetic mechanism of this increase is not yet fully elucidated,but several theories have been *** of antral Dcells in ***-positive patients causes a suppression of somatostatin secretion that,in turn,stimulates increased secretin *** stimulates pancreatic growth and thus increases the risk of ***,***,as a part of microbiome dysbiosis and the so-called oncobiome,is proven to be associated with pancreatic adenocarcinoma development via the promotion of cellular *** role of *** in the inflammation characteristic of autoimmune pancreatitis seems to be explained by a mechanism of molecular mimicry among several proteins(mostly enzymes)of *** and pancreatic *** with autoimmune pancreatitis often show positivity for antibodies against *** ***,as a part of microbiome dysbiosis,also

关键词： Helicobacter pylori Pancreatic cancer Autoimmune pancreatitis Carcinogenesis Microbiome Molecular mimicry

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Positive association between forest management, environmental change, and forest bird abundance

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Forest Ecosystems 2019年第1期6卷 26-37页

作者： Ernst Detlef Schulze Dylan Craven Andrew M.Durso jiri Reif Marcus Guderle Franz Kroiher Petra Hennig Anne Weiserbs Peter Schall Christian Ammer Nico Eisenhauer Max Planck Institute for Biogeochemistry Biodiversity Macroecology and Biogeography Faculty of forest Science and Forest Ecology University G?ttingen Institute of Environmental Studies Faculty of ScienceCharles University Thünen-Institute for Forest Ecosystems Aves-Natagora Départment Etudes Silviculture and Forest Ecology of the temperate Zones University of G?ttingen German Centre for Integrative Biodiversity Research (iDiv) and Leipzig University Institute of Biology

Background: The global decrease in wildlife populations, especially birds, is mainly due to land use change and increasing intensity of land use(Parmesan and Yohe 2003). However, impacts of management tools to mitigate biodiversity loss at regional and global scales are less apparent in forest regions that have a constant forest area,and which did not suffer from habitat degradation, and where forests are sustainably managed, such as in Central Europe or the northeastern USA. A biodiversity assessment for Germany suggested, for example, that bird populations were constant(Bundesamt für Naturschutz 2015).Results: This study shows that changes in the environment and in forest management over the past 45 years have had a significant, positive effect on the abundance of non-migratory forest bird species in Central Europe. Economy(timber prices and GDP), forest management(timber harvest and mixed forest area), and environmental factors(atmospheric CO_2 concentration and nitrogen deposition) were investigated together with changes in abundances of migratory and non-migratory forest birds using partial least squares path modeling. Climate change, resulting in longer seasons and milder winters, and forest management, promoting tree diversity, were significantly positively related to the abundance of non-migratory forest birds and explained 92% of the variation in their abundance in Europe. Regionally-migrating forest birds had stable populations with large variation, while birds migrating across continents declined in recent decades, suggesting significant, contrasting changes in bird populations in Europe. In northeastern North America we also found evidence that non-migratory forests have experienced long-term increases in abundance, and this increase was related to management. The increase of populations of nonmigratory forest birds in Europe and North America is associated with an increase in structural diversity and disturbances at the landscape ***: Our

关键词： Climate change Birds Biodiversity Timber harvest Econo my Forest management

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Retromer Subunits VPS35A and VPS29 Mediate Prevacuolar Compartment （PVC） Function in Arabidopsis

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Molecular Plant 2013年第6期6卷 1849-1862页

作者： Tomasz Nodzynski Mugurel I. Ferarub Sibylle Hirsch Riet De Rycke Claudiu Niculaes Wout Boerjan Jelle Van Leene Geert De Jaeger Steffen Vanneste jiri Friml Mendel Centre for Plant Genomics and Proteomics Central European Institute of Technology (CEITEC) Masaryk University (MU) Kamenice 5 CZ-625 00Brno Czech Republic Department of Plant Systems Biology VIB and Department of Plant Biotechnology and Bioinformatics Ghent University 9052 Gent Belgium Institute of Science and Technology Austria (IST Austria) Am Campus 1 3400 Klosterneuburg Austria Present address: Department of Applied Genetics and Cell Biology University of Natural Resources and Life Sciences (BOKU) 1190 Vienna Austria Present address: Institute of Plant Biology University of Zurich 8008 ZCirich Switzerland

Intracellular protein routing is mediated by vesicular transport which is tightly regulated in eukaryotes. The protein and lipid homeostasis depends on coordinated delivery of de novo synthesized or recycled cargoes to the plasma membrane by exocytosis and their subsequent removal by rerouting them for recycling or degradation. Here, we report the characterization of protein affected trafficking 3 （pat3） mutant that we identified by an epifluorescence-based for- ward genetic screen for mutants defective in subcellular distribution of Arabidopsis auxin transporter PIN1-GFR While pat3 displays largely normal plant morphology and development in nutrient-rich conditions, it shows strong ectopic intracellular accumulations of different plasma membrane cargoes in structures that resemble prevacuolar compart- ments （PVC） with an aberrant morphology. Genetic mapping revealed that pat3 is defective in vacuolar protein sorting 35A （VPS35A）, a putative subunit of the retromer complex that mediates retrograde trafficking between the PVC and trans-Golgi network. Similarly, a mutant defective in another retromer subunit, vps29, shows comparable subcellular defects in PVC morphology and protein accumulation. Thus, our data provide evidence that the retromer components VPS35A and VPS29 are essential for normal PVC morphology and normal trafficking of plasma membrane proteins in plants. In addition, we show that, out of the three VPS35 retromer subunits present in Arabidopsis thaliana genome, the VPS35 homolog A plays a prevailing role in trafficking to the lyric vacuole, presenting another level of complexity in the retromer-dependent vacuolar sorting.

关键词： retromer VPS35 VPS29 prevacuolar compartment (PVC) vacuolar trafficking Arabidopsis thaliana.

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Development of New Tool Steels for Forging Dies

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材料科学与工程（中英文B版） 2011年第5期1卷 636-640页

作者： Pavel Suchmann jiri Krejcik Ludvik Martinek COMTES PHT a.s. Prumyslova 995 33441 Dobrany Czech Republic SVUM a.s. areal VU Podnikatelska 565 190 11 Praha 9 Bechovice Czech Republic ZDAS a. s. Strojirenska 6 591 71 Zdar nad Sazavou Czech Republic

Forging dies used for close dies forging of small and middle-sized steel parts are required to possess an optimum combination of strength, toughness, resistance to tempering and other properties. Typical alloying elements include chromium, vanadium, molybdenum, or tungsten. This alloying strategy can be found e. g. in the steels 1.2343, 1.2344 and others. The present paper describes new alloying concepts of steels for forging dies based on the 1.2343 steel. By increasing the carbon content, and in some cases increasing the tungsten level and adding niobium, using a sufficient metallurgical procedure and subsequent special process for ingot forging, the hardness, toughness and wear resistance of the steel have been enhanced significantly. The lifetime of dies made from the improved steels is about 50%-100% longer than that of dies made from conventional 1.2343 steel.

关键词：工具钢锻造模具锻模合金元素最佳组合冶金过程模具钢碳含量

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Does combined therapy of curcumin and epigallocatechin gallate have a synergistic neuroprotective effect against spinal cord injury？

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Neural Regeneration Research 2018年第1期13卷 119-127页

作者： jiri Ruzicka Lucia Machova Urdzikova Barbora Svobodova Anubhav G. Amin Kristyna Karova Jana Dubisova Kristyna Zaviskova Sarka Kubinova Meic Schmidt Meena Jhanwar-Uniyal Pavla Jendelova Institute of Experimental Medicine Academy of Sciences of the Czech Republic Videfiska Prague Czech Republic Department of Neuroscience Charles University Second Faculty of Medicine Prague Czech Republic New York Medical College Valhalla NY USA

Systematic inflammatory response after spinal cord injury （SCI） is one of the factors leading to lesion development and a profound degree of functional loss. Anti-inflammatory compounds, such as curcumin and epigallocatechin gallate （EGCG） are known for their neuroprotective effects. In this study, we investigated the effect of combined therapy of curcumin and EGCG in a rat model of acute SCI induced by balloon compression. Immediately after SCI, rats received curcumin, EGCG, curcumin ＋ EGCG or saline [daily intraperitoneal doses （curcumin, 6 mg/kg; EGCG 17 mg/kg）] and weekly intramuscular doses （curcumin,60 mg/kg; EGCG 17 mg/kg）] for 28 days. Rats were evaluated using behavioral tests （the Basso, Beattie, and Bresnahan （BBB） open-field locomotor test, flat beam test）. Spinal cord tissue was analyzed using histological methods （Luxol Blue-cresyl violet staining） and mmunohistochemistry （anti-glial fibrillary acidic protein, anti-growth associated protein 43）. Cytokine levels （interleukin-1β, interleukin-4, interleukin-2,interleukin-6, macrophage inflammatory protein 1-alpha, and RANTES） were measured using Luminex assay. Quantitative polymerase chain reaction was performed to determine the relative expression of genes （Sort1, Fgf2, Irf5, Mrc1, Olig2, Casp3, Gap43, Gfap, Vegf, NfκB, Cntf） related to regenerative processes in injured spinal cord. We found that all treatments displayed significant behavioral recovery, with no obvious synergistic effect after combined therapy of curcumin and ECGC. Curcumin and EGCG alone or in combination increased axonal sprouting, decreased glial scar formation, and altered the levels of macrophage inflammatory protein 1-alpha, interleukin-1β, interleukin-4 and interleukin-6 cytokines. These results imply that although the expected synergistic response of this combined therapy was less obvious, aspects of tissue regeneration and immune responses in severe SCI were evident.

关键词： spinal cord injury epigallocatechin gallate curcumin inflammatory response neural regeneration

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纳米纤维复合结构空气过滤材料性能研究

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纺织学报 2023年第5期44卷 77-83页

作者：胡蝶飞王琰姚菊明 DAS Ripon MILITKY jiri VENKATARAMAN Mohanapriya 祝国成浙江理工大学纺织科学与工程学院浙江杭州310018 浙江省现代纺织技术创新中心(鉴湖实验室) 浙江绍兴312000 浙江理工大学浙江-捷克先进纤维材料联合实验室浙江杭州310018 浙江理工大学材料科学与工程学院浙江杭州310018 宁波大学材料科学与化学工程学院浙江宁波315201 利贝雷茨理工大学纺织工程学院捷克利贝雷茨46117

为深入探究影响纳米纤维膜过滤机制以开发和应用高端空气过滤材料,分析了静电纺纳米纤维膜和聚四氟乙烯拉伸膜与非织造布复合的空气过滤材料结构与性能,分别对其形貌、热力学性能、透气性等进行了分析与评价。实验结果表明,过滤材料本... 详细信息

为深入探究影响纳米纤维膜过滤机制以开发和应用高端空气过滤材料,分析了静电纺纳米纤维膜和聚四氟乙烯拉伸膜与非织造布复合的空气过滤材料结构与性能,分别对其形貌、热力学性能、透气性等进行了分析与评价。实验结果表明,过滤材料本身的结构和纤维直径以及表面电势对其过滤性能影响显著,纤维直径越细,膜孔径越小,纤维间的结构会相对紧密,从而导致透气性较差,过滤效率更好。其中表面电势是影响材料过滤效率的主要因素,表面电势越高,过滤效果越好。静电纺锦纶6纳米纤维膜/聚酯纤维非织造布(PA6/PET)复合空气过滤材料表面电势最高,达1.414 kV,其过滤效率最佳,达到99.57%。PA6/木浆纸复合过滤材料表面电势最小,为0.07 kV,过滤效果仅为22.28%。

关键词：静电纺纳米纤维膜聚四氟乙烯复合结构过滤效率非织造布

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37th Annual Meeting of the European Radiation Research Society

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2009年

作者： Skopek jiri.

来源：畅想之星图书光盘版（南... 评论

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Back-Propagation and K-Means Algorithms Comparison

Back-Propagation and K-Means Algorithms Comparison

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第八届国际信号处理国际会议

作者： Vladislav Skorpil jiri Stastny Department of Telecommunications Brno University of TechnologyPurkynova 118612 00 BrnoCzech Republic Department of Automation and Computer Science Brno University of Technology Purkynova 118612 00 BrnoCzech Republic

＜正＞The paper describes the application of algorithms for object classification by using artificial neural networks. The MLP （Multi Layer Perceptron） and RBF （Radial Basis Function） neural networks were used. We compared results obtained by a using of learning algorithms Back-Propagation （BP） and K-Means. The real technological scene for object classification was simulated with digitization of two-dimensional pictures. The principles and algorithms given below have been used in an application that was developed at Brno University of Technology.

关键词： Image Processing Back-Propagation Algorithm K-Means Algorithm.

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Three-dimensional visualization of planta clathrin-coated vesicles at ultrastructural resolution

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Molecular Plant 2022年第10期15卷 1533-1542页

作者： Alexander Johnson Walter A.Kaufmann Christoph Sommer Tommaso Costanzo Dana A.Dahhan Sebastian Y.Bednarek jiri Friml Institute of Science and Technology Austria(ISTA) 3400KlosterneuburgAustria UW-Madison Department of Biochemistry433 Babcock Dr.MadisonWI 53706USA

Biological systems are the sum of their dynamic three-dimensional(3D)***,it is critical to study biological structures in 3D and at high resolution to gain insights into their physiological *** microscopy of metal replicas of unroofed cells and isolated organelles has been a key technique to visualize intracellular structures at nanometer ***,many of these methods require specialized equipment and personnel to complete ***,we present novel accessible methods to analyze biological structures in unroofed cells and biochemically isolated organelles in 3D and at nanometer resolution,focusing on Arabidopsis clathrin-coated vesicles(CCVs).While CCVs are essential trafficking organelles,their detailed structural information is lacking due to their poor preservation when observed via classical electron microscopy protocols ***,we establish a method to visualize CCVs in unroofed cells using scanning transmission electron microscopy tomography,providing sufficient resolution to define the clathrin coat ***,the samples are prepared directly on electron microscopy grids,removing the requirement to use extremely corrosive acids,thereby enabling the use of this method in any electron microscopy ***,we demonstrate that this standardized sample preparation allows the direct comparison of isolated CCV samples with those visualized in ***,to facilitate the high-throughput and robust screening of metal replicated samples,we provide a deep learning analysis method to screen the“pseudo 3D”morphologies of CCVs imaged with 2D ***,our work establishes accessible ways to examine the 3D structure of biological samples and provide novel insights into the structure of plant CCVs.

关键词： Arabidopsis thaliana clathrin-coated vesicles STEM tomography electron microscopy clathrin-mediated endocytosis

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