检索结果-南通市图书馆

Small intestinal bacterial overgrowth syndrome

World Journal of Gastroenterology 2010年第24期16卷 2978-2990页

作者： Jan Bures jiri cyrany Darina Kohoutova Miroslav Frstl Stanislav Rejchrt Jaroslav Kvetina Viktor Vorisek Marcela Kopacova 2nd Department of Medicine Charles University in Praha Faculty of Medicine at Hradec Kralove University Teaching Hospital Institute of Clinical Microbiology Charles University in Praha Faculty of Medicine at Hradec Kralove University Teaching Hospital Institute of Experimental Biopharmaceutics Joint Research Centre of Czech Academy of Sciences and PRO.MED.CS Praha a.s. Institute of Clinical Biochemistry and Diagnostics Charles University in Praha Faculty of Medicine at Hradec Kralove University Teaching Hospital

Human intestinal microbiota create a complex polymi-crobial ecology. This is characterised by its high population density, wide diversity and complexity of interaction. Any dysbalance of this complex intestinal microbiome, both qualitative and quantitative, might have serious health consequence for a macro-organism, including small intestinal bacterial overgrowth syndrome (SIBO).SIBO is defined as an increase in the number and/or alteration in the type of bacteria in the upper gastro-intestinal tract. There are several endogenous defence mechanisms for preventing bacterial overgrowth: gastric acid secretion, intestinal motility, intact ileo-caecal valve, immunoglobulins within intestinal secretion and bacte-riostatic properties of pancreatic and biliary secretion. Aetiology of SIBO is usually complex, associated with disorders of protective antibacterial mechanisms (e.g. achlorhydria, pancreatic exocrine insuff iciency, immuno-deficiency syndromes), anatomical abnormalities (e.g. small intestinal obstruction, diverticula, f istulae, surgical blind loop, previous ileo-caecal resections) and/or motility disorders (e.g. scleroderma, autonomic neuropathy in diabetes mellitus, post-radiation enteropathy, small intestinal pseudo-obstruction). In some patients more than one factor may be involved. Symptoms related to SIBO are bloating, diarrhoea, malabsorption, weight loss and malnutrition. The gold standard for diagnosing SIBO is still microbial investigation of jejunal aspirates. Noninvasive hydrogen and methane breath tests are most commonly used for the diagnosis of SIBO using glucose or lactulose. Therapy for SIBO must be com-plex, addressing all causes, symptoms and complica-tions, and fully individualised. It should include treatment of the underlying disease, nutritional support and cyclical gastro-intestinal selective antibiotics. Prognosis is usually serious, determined mostly by the underlying disease that led to SIBO.

关键词： Bacterial overgrowth Breath test Hydrogen Methane Small intestine

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bacteriocinogeny in experimental pigs treated with indomethacin and Escherichia coli Nissle

引用

World Journal of Gastroenterology 2011年第5期17卷 609-617页

作者： Jan Bures David Smajs Jaroslav Kvetina Miroslav Frstl Jan Smarda Darina Kohoutova Martin Kunes jiri cyrany Ilja Tacheci Stanislav Rejchrt jirina Lesna Viktor Vorisek Marcela Kopacova 2nd Department of Medicine Charles University in PrahaFaculty of Medicine at Hradec KraloveUniversity Teaching Hospital500 05 Hradec KraloveCzech Republic Department of Biology Masaryk UniversityFaculty of Medicine625 00 BrnoCzech Republic Institute of Experimental Biopharmaceutics Joint Research Centre of Czech Academy of Sciences and PRO.MED.CS Praha a.s.500 02 Hradec KraloveCzech Republic Institute of Clinical Microbiology Charles University in PrahaFaculty of Medicine at Hradec KraloveUniversity Teaching Hospital500 05 Hradec KraloveCzech Republic Institute of Clinical Biochemistry and Diagnostics Charles University in PrahaFaculty of Medicine at Hradec KraloveUniversity Teaching Hospital500 05 Hradec KraloveCzech Republic

AIM:To evaluate bacteriocinogeny in short-term highdose indomethacin administration with or without probiotic Escherichia coli Nissle 1917(EcN) in experimental ***:Twenty-four pigs entered the study:Group A(controls),Group B(probiotics alone),Group C(indomethacin alone) and Group D(probiotics and indomethacin).EcN(3.5 × 1010 bacteria/d for 14 d) and/or indomethacin(15 mg/kg per day for 10 d) were administrated *** smears before and smears from the small and large intestine were taken from all *** production was determined with 6 different indicator strains;all strains were polymerase chain reaction tested for the presence of 29 individual bacteriocinencoding ***:The general microbiota profile was rather uniform in all animals but there was a broad diversity in coliform bacteria(parallel genotypes A,B1,B2 and D found).In total,637 bacterial strains were tested,mostly Escherichia coli(***).There was a higher incidence of *** strains among samples taken from the jejunum and ileum compared to that of the colon and rectum indicating predominance of *** strains in the large *** was found in 24/77(31%) before and in 155/560(28%) isolated bacteria at the end of the ***,13 individual bacteriocin types(out of 29 tested) were identified among investigated *** of four *** genotypes was equally distributed in all groups of *** strains,with majority of genotype A(ranging from 81% to 88%).The following types of bacteriocins were most commonly revealed:colicins Ia/Ib(44%),microcin V(18%),colicin E1(16%) and microcin H47(6%).There was a difference in bacteriocinogeny between control group A(52/149,35%) and groups with treatment at the end of the study:B:31/122(25%,P = 0.120);C:43/155(28%,P = 0.222);D:29/134(22%,P = 0.020).There was a significantly lower prevalence of colicin Ib,microcins H47 and V(probiotics group,P < 0.001),colicin E1 and microcin H47(indomethacin

关键词：大肠杆菌消炎痛实验猪治疗类细菌素聚合酶链反应共同管理益生菌

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Buried bumper syndrome:A complication of percutaneousendoscopic gastrostomy

引用

World Journal of Gastroenterology 2016年第2期22卷 618-627页

作者： jiri cyrany Stanislav Rejchrt Marcela Kopacova Jan Bures nd Department of Internal Medicine-Gastroenterology University Hospital Hradec Kralove50005 Hradec KraloveCzech Republic Faculty of Medicine in Hradec Kralove Charles University in Prague50005 Hradec KraloveCzech Republic

Percutaneous endoscopic gastrostomy(PEG) is a widely used method of nutrition delivery for patients with longterm insufficiency of oral intake. The PEG complication rate varies from 0.4% to 22.5% of cases, with minor complications being three times more frequent. Buried bumper syndrome(BBS) is a severe complication of this method, in which the internal fixation device migrates alongside the tract of the stoma outside the stomach. Excessive compression of tissue between the external and internal fixation device of the gastrostomy tube is considered the main etiological factor leading to BBS. Incidence of BBS is estimated at around 1%(0.3%-2.4%). Inability to insert, loss of patency and leakage around the PEG tube are considered to be a typical symptomatic triad. Gastroscopy is indicated in all cases in which BBS is suspected. The depth of disc migration in relation to the lamina muscularis propria of the stomach is critical for further therapy and can be estimated by endoscopic or transabdominal ultrasound. BBS can be complicated by gastrointestinal bleeding, perforation, peritonitis, intra-abdominal and abdominal wall abscesses, or phlegmon, and these complications can lead to fatal outcomes. The most important preventive measure is adequate positioning of the external bolster. A conservative approach should be applied only in patients with high operative risk and dismal prognosis. Choice of the method of release is based on the type of the PEG set and depth of disc migration. A disc retained inside the stomach and completely covered by the overgrowing tissue can be released using some type of endoscopic dissection technique(needle knife, argon plasma coagulation, or papillotome through the cannula). Proper patient selection and dissection of the overgrowing tissue are the major determinants for successful endoscopic therapy. A disc localized out of the stomach(lamina muscularis propria) should be treated by a surgeon.

关键词： Buried bumper syndrome Percutaneous endoscopic gastrostomy Endoscopy Complication Enteral nutrition

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Longitudinal molecular characterization of endoscopic specimens from colorectal lesions

引用

World Journal of Gastroenterology 2016年第20期22卷 4936-4945页

作者： Petra Minarikova Lucie Benesova Tereza Halkova Barbora Belsanova Stepan Suchanek jiri cyrany Inna Tuckova Jan Bures Miroslav Zavoral Marek Minarik Department of Internal Medicine 1st Faculty of Medicine of Charles University and Military University Hospital Genomac Research Institute Centre for Applied Genomics of Solid Tumors 2nd Department of Internal Medicine - Gastroenterology Charles University Faculty of Medicine at Hradec Kralove University Teaching Hospital

AIM: To compare molecular profiles of proximal colon, distal colon and rectum in large adenomas, early and late carcinomas. To assess feasibility of testing directed at molecular markers from this study in routine clinical practice. METHODS: A prospective 3-year study has resulted in the acquisition of samples from 159 large adenomas and 138 carcinomas along with associated clinical parameters including localization, grade and histological type for adenomas and localization and stage for carcinomas. A complex molecular phenotyping has been performed using multiplex ligation-dependent probe amplification technique for the evaluation of Cp G-islandmethylator phenotype(CIMP), PCR fragment analysis for detection of microsatellite instability and denaturing capillary electrophoresis for sensitive detection of somatic mutations in KRAS, BRAF, TP53 and APC ***: Molecular types according to previously introduced Jass classification have been evaluated for large adenomas and early and late carcinomas. An increase in CIMP+ type, eventually accompanied with KRAS mutations, was notable between large adenomas and early carcinomas. As expected, the longitudinal observations revealed a correlation of the CIMP+/BRAF + type with proximal location. CONCLUSION: Prospective molecular classification of tissue specimens is feasible in routine endoscopy practice. Increased frequency of some molecular types corresponds to the developmental stages of colorectal tumors. As expected, a clear distinction is notable for tumors located in proximal colon supposedly arising from the serrated(methylation) pathway.

关键词： Colorectal cancer Cp G-island methylator phenotype DNA Microsatellite instability BRAF

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：