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Selenomethionine alleviates chronic heat stress-induced breast muscle injury and poor meat quality in broilers via relieving mitochondrial dysfunction and endoplasmic reticulum stress

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Animal Nutrition 2024年第1期16卷 363-375页

作者： jinzhong Jing Jiayi Wang Xiaoyu Xiang Shenggang Yin Jiayong Tang Longqiong Wang Gang Jia Guangmang Liu Xiaoling Chen Gang tian Jingyi Cai Bo Kang Lianqiang Che Hua Zhao Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education of China Ministry of Agriculture and Rural Affairsof Sichuan ProvinceAnimal Nutrition InstituteSichuan Agricultural University College of Animal Science and Technology Sichuan Agricultural University

In the present study,the chronic heat stress （CHS） broiler model was developed to investigate the potential protection mechanism of organic selenium （selenomethionine,SeMet） on CHS-induced skeletal muscle growth retardation and poor meat quality.Four hundred Arbor Acres male broilers （680±70 g,21 d old） were grouped into 5 treatments with 8 replicates of 10 broilers per replicate.Broilers in the control group were raised in a thermoneutral environment （22±2°C） and fed with a basal diet.The other four treatments were exposed to hyperthermic conditions （33±2°C,24 h in each day） and fed on the basal diet supplied with SeMet at 0.0,0.2,0.4,and 0.6 mg Se/kg,respectively,for 21 d.Results showed that CHS reduced （P<0.05） the growth performance,decreased （P<0.05） the breast muscle weight and impaired the meat quality of breast muscle in broilers.CHS induced protein metabolic disorder in breast muscle,which increased （P<0.05） the expression of caspase 3,caspase 8,caspase 9 and ubiquitin proteasome system related genes,while decreased the protein expression of P-4EBP1.CHS also decreased the antioxidant capacity and induced mitochondrial stress and endoplasmic reticulum （ER） stress in breast muscle,which increased （P<0.05） the ROS levels,decreased the concentration of ATP,increased the protein expression of HSP60 and CLPX,and increased （P<0.05） the expression of ER stress biomarkers.Dietary SeMet supplementation linearly increased （P<0.05） breast muscle Se concentration and exhibited protective effects via up-regulating the expression of the selenotranscriptome and several key selenoproteins,which increased （P<0.05） body weight,improved meat quality,enhanced antioxidant capacity and mitigated mitochondrial stress and ER stress.What's more,SeMet suppressed protein degradation and improved protein biosynthesis though inhibiting the caspase and ubiquitin proteasome system and promoting the mTOR-4EBP1 pathway.In conclusion,dietary SeMet supplementation increases the expression of several key selenoproteins,alleviates mitochondr

关键词： Broiler Chronic heat stress Skeletal muscle Meat quality Mitochondrial dysfunction Endoplasmic reticulum stress

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Formate as a supplementary substrate facilitates sugar metabolism and solvent production by Clostridium beijerinckii NCIMB 8052

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Synthetic and Systems Biotechnology 2023年第2期8卷 196-205页

作者： Ran Zhao Wenyue Dong Chen Yang Weihong Jiang jinzhong tian Yang Gu CAS-Key Laboratory of Synthetic Biology CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyChinese Academy of SciencesShanghai200032China University of Chinese Academy of Sciences Beijing100049China

Microbial utilization and conversion of organic one-carbon compounds,such as formate and methanol that can be easily produced from CO_(2),has emerged as an attractive approach for biorefinery.In this study,we discovered Clostridium beijerinckii NCIMB 8052,a typical solventogenic Clostridium strain,to be a native formate-utilizing bacterium.^(13)C isotope analysis showed that formate could be metabolized via both assimilation and dissimi-lation pathways in C.beijerinckii NCIMB 8052.Notably,the use of formate as the supplementary substrate by this strain could significantly enhance its glucose consumption and ABE(acetone-butanol-ethanol)production,largely due to the up-regulation of genes responsible for glycolysis and glucose transport under formate stress.Based on these findings,we further improved formate tolerance of C.beijerinckii NCIMB 8052 by adaptive lab-oratory evolution,generating an evolved strain Cbei-FA01.The Cbei-FA01 strain could produce 23.0 g/L of ABE solvents using glucose and formate as dual substrates,~50%higher than that of the wild-type strain under the same condition.Moreover,such a promotion effect of formate on ABE production by Cbei-FA01 was also observed in fermenting a glucose-xylose mixture.This work reveals a previously unreported role of formate in biological ABE production,providing a new approach to utilize this one-carbon source.

关键词： Formate utilization Clostridium beijerinckii Metabolic pathways Adaptive laboratory evolution ABE solvents

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Correlation between the mechanical properties and the<110>texture in a hot-rolled nearβtitanium alloy

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Journal of Materials Science & Technology 2022年第2期97卷 165-168页

作者： Ruifeng Dong Xiaoyang Zhang Chenhui Li Yuhong Zhao jinzhong tian Li Wu Hua Hou School of Materials Science and Engineering North University of ChinaTaiyuanShanxi 030051PR China State Key Laboratory of Solidification ProcessingNorthwestern Polytechnical UniversityXi’anShaanxi 710072PR China State Key Laboratory of Solidification Processing Northwestern Polytechnical UniversityXi’anShaanxi 710072PR China

1.Introduction Nearβtitanium alloys have been considered as a potential material to fabricate high-strength fasteners in aerospace field owing to their high specific strength,good crack and fatigue resistance[1,2].It is well known that the processing technology determines the microstructure,and the microstructure eventually correlates with the performance of alloys.

关键词： microstructure strength alloy

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黑豆纳豆激酶粗提取物的免疫调节作用

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中国食品学报 2024年第1期24卷 31-40页

作者：田玥玮张永坡高春艳赵晋忠杜维俊山西农业大学基础部山西晋中030801 山西农业大学农学院山西晋中030801

以壶关大黑豆为原料发酵纳豆,分离得到黑豆纳豆激酶粗提取物(BNCE),对其结构进行初探,测定其酶活力和溶栓能力,并研究BNCE的免疫调节作用。通过红外光谱分析和扫描电镜对BNCE结构进行鉴定;分别使用纤维蛋白平板法和动物血块法测定BNCE... 详细信息

以壶关大黑豆为原料发酵纳豆,分离得到黑豆纳豆激酶粗提取物(BNCE),对其结构进行初探,测定其酶活力和溶栓能力,并研究BNCE的免疫调节作用。通过红外光谱分析和扫描电镜对BNCE结构进行鉴定;分别使用纤维蛋白平板法和动物血块法测定BNCE的酶活及其对小鼠血块的体外溶栓作用;用MTT比色法探究BNCE对RAW264.7细胞增殖的影响;中性红染色法测定BNCE对RAW264.7细胞吞噬率的影响;ELISA法检测BNCE对RAW264.7细胞分泌IL-6、TNF-α和IL-1β的影响。结果表明,BNCE具有蛋白质的化学结构,表观呈现大小不同且无规则的碎片形状,具有一定的纤溶活性和体外溶栓作用。BNCE可以促进正常状态以及LPS激活状态下RAW264.7细胞的增殖,增强其吞噬率,并且有浓度依赖性。另外,BNCE还可以促进正常状态下的RAW264.7细胞TNF-α和IL-1β的分泌,抑制IL-6的分泌;也能抑制LPS激活状态下RAW264.7细胞IL-6、TNF-α和IL-1β的分泌。因此,BNCE具有一定的免疫调节作用,在食品工业上可以作为保健食品的一种优质原料。

关键词：黑豆纳豆激酶溶栓作用 RAW264.7细胞免疫调节

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Multiplex genome editing using a dCas9-cytidine deaminase fusion in Streptomyces

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Science China(Life Sciences) 2020年第7期63卷 1053-1062页

作者： Yawei Zhao jinzhong tian Guosong Zheng Jun Chen Chuanwen Sun Zhongyi Yang Andrei A.Zimin Weihong Jiang Zixin Deng Zhijun Wang Yinhua Lu College of Life Sciences.Shanghai Normal University Shanghai 200234China State Key Laboratory of Microbial Metabolism School of Life Sciences and Biotechnology Shanghai Jiao Tong UniversityShanghai 200030China Key Laboratory of Synthetic Biology CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China School of Life Science Taizhou UniversityTaizhou 318000China Institute of Biochemistry and Physiology of Microorganisms Russian Academy of SciencesPushchino 142290Russia Shanghai Engineering Research Center of Plant Germplasm Resources College of Life SciencesShanghai Normal UniversityShanghai 200234China

CRISPR/Cas-mediated genome editing has greatly facilitated the study of gene function in Streptomyces. However, it could not be efficiently employed in streptomycetes with low homologous recombination(HR) ability. Here, a deaminase-assisted base editor d Cas9-CDA-ULstr was developed in Streptomyces, which comprises the nuclease-deficient Cas9(dCas9), the cytidine deaminase from Petromyzon marinus(PmCDA1), the uracil DNA glycosylase inhibitor(UGI) and the protein degradation tag(LVA tag). Using d Cas9-CDA-ULstr , we achieved single-, double-and triple-point mutations(cytosine-to-thymine substitutions)at target sites in Streptomyces coelicolor with efficiency up to 100%, 60% and 20%, respectively. This base editor was also demonstrated to be highly efficient for base editing in the industrial strain, Streptomyces rapamycinicus, which produces the immunosuppressive agent rapamycin. Compared with base editors derived from the cytidine deaminase rAPOBEC1, the PmCDA1-assisted base editor dCas9-CDA-ULstr could edit cytosines preceded by guanosines with high efficiency, which is a great advantage for editing Streptomyces genomes(with high GC content). Collectively, the base editor dCas9-CDA-ULstr could be employed for efficient multiplex genome editing in Streptomyces. Since the d Cas9-CDA-ULstr -based genome editing is independent of HR-mediated DNA repair, we believe this technology will greatly facilitate functional genome research and metabolic engineering in Streptomyces strains with weak HR ability.

关键词： base editors dCas9-CDA-ULstr multiplex editing Streptomyces

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基于集合匹配尺度的邻域集合概率法及其在梅雨锋暴雨预报中的适用性

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热带气象学报 2024年第2期40卷 313-325页

作者：徐渊闵锦忠庄潇然陈云辉张璐田高山江西省气象台江西南昌330096 南京信息工程大学气象灾害教育部重点实验室/气象灾害预报预警与评估协同创新中心江苏南京210044 江苏省气象台江苏南京210008 民航西南空管局气象中心四川成都610202

概率预报是由集合预报衍生、包含不确定性信息的客观产品,对业务决策服务有重要的参考价值。传统的邻域集合概率法中,邻域半径固定不变,不符合实际天气过程中牵涉甚广的尺度谱。为此引入基于集合匹配尺度的邻域集合概率法(Neighborhood ... 详细信息

概率预报是由集合预报衍生、包含不确定性信息的客观产品,对业务决策服务有重要的参考价值。传统的邻域集合概率法中,邻域半径固定不变,不符合实际天气过程中牵涉甚广的尺度谱。为此引入基于集合匹配尺度的邻域集合概率法(Neighborhood Ensemble Probability based on Ensemble Agreement Scale,EAS_NEP),并在中国南方典型的梅雨锋暴雨中开展准确性和预报技巧的定量检验评估,以期验证该方法在此类过程中的适用性,并促进其在实际业务中的推广使用。联合扰动初始场、侧边界和物理过程所得到的集合预报能较好地表征实际的预报不确定性,进一步在此基础上比较了格点概率法、不同半径的邻域集合概率法以及EAS_NEP的优劣。试验结果表明,EAS_NEP能根据集合成员间的一致性程度,自适应地调整邻域半径,其在集中型降水中所确定的邻域半径通常大于分散型降水。动态调整的邻域半径既避免了半径过大时的过度平滑与关键信息丢失,又消除了半径较小所带来的奇异点,其空间分布呈阶梯型,空间连续性更优。此外,BS(布莱尔评分)、FSS(分数技巧评分)和ROC曲线(相对作用特征曲线)等定量评估结果也体现出EAS_NEP相比传统方法正的预报技巧,尤其是在分散型降水和高阈值检验时优势更明显。以上结果表明,EAS_NEP在梅雨锋暴雨的预报中具有较好的应用前景,运用在业务中能有效提升概率预报质量。

关键词：概率预报集合预报集合匹配尺度邻域集合概率梅雨锋暴雨

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First-principles Study of Electronic Structural and Mechanical Properties of Mg_(x)La(x=1,2,3)Compounds under Pressure

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Journal of Wuhan University of Technology(Materials Science) 2023年第1期38卷 192-198页

作者：李艳赵宇宏 YANG Xiaomin tian jinzhong College of Materials Science and Engineering North University of ChinaTaiyuan 030051China

The effects of pressure on structural,elastic and electronic properties of Mg_(x)La(x=1,2,3)compounds are investigated by using CASTEP program based on the density functional theory.The calculated equilibrium lattice parameters at zero pressure agree well with available experimental and theoretical values.The calculated DOS show that the structure of these compounds remains mechanically stable and structural phase transformation is not induced with increasing pressure from 0 to 30 GPa,and their structural stability increases with pressure.The ductility of MgLa can be improved by increasing pressure,which is the same as Mg_(2)La in 0-20 GPa,while brittle behavior turns into ductile behavior in 0-5 GPa for Mg_(3)La.The resistance to volume deformation of Mg_(x)La(x=1,2,3)compounds can be improved as the pressure increases.The shear deformation resistance and elastic stiffness of Mg_(3)La can be enhanced by rising pressure,but MgLa and Mg_(2)La increase first and then decrease when pressure is up to 25 GPa.In addition,the three compounds exhibit the elastic anisotropy with pressure.

关键词： Mg-La alloys elastic properties electronic structure first-principles

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薄板坯连铸结晶器在线调宽技术的应用

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山西冶金 2024年第4期47卷 200-202页

作者：张李鹏吴志会吴金钟田振金柱元日照钢铁控股集团有限公司山东日照276800

日照钢铁公司薄板坯连铸连轧产线以其短流程、低能耗、高产量的特点而得到快速发展,该产线以生产低碳低硅钢、耐候钢、汽车钢、中碳钢、高碳钢等系列1250~1500 mm断面规格为主,带钢尺寸精度控制高。由于连铸结晶器出口宽度固定,单浇次... 详细信息

日照钢铁公司薄板坯连铸连轧产线以其短流程、低能耗、高产量的特点而得到快速发展,该产线以生产低碳低硅钢、耐候钢、汽车钢、中碳钢、高碳钢等系列1250~1500 mm断面规格为主,带钢尺寸精度控制高。由于连铸结晶器出口宽度固定,单浇次内带钢宽度单一,通过应用结晶器在线调宽技术,实现了单浇次内多种规格宽度生产,实现了薄板坯连铸连轧技术的新突破。

关键词：薄板坯结晶器在线调宽

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超声探伤仪计量耦合剂效能数值模拟及验证

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上海计量测试 2024年第2期51卷 17-19,23页

作者：雪宇超屈建峰王翰博田浩张金钟宝鸡市质量技术检验检测中心

采用COMSOL Multiphysics有限元仿真软件,分别分析三种常用工业超声探伤耦合剂水、机油、甘油对计量分辨力波形结果的增益效果,并针对耦合剂的厚度在0.1 mm、0.3 mm、0.5 mm、1.0 mm、1.3 mm时对响应波形影响进行分析,结果表明:耦合剂... 详细信息

采用COMSOL Multiphysics有限元仿真软件,分别分析三种常用工业超声探伤耦合剂水、机油、甘油对计量分辨力波形结果的增益效果,并针对耦合剂的厚度在0.1 mm、0.3 mm、0.5 mm、1.0 mm、1.3 mm时对响应波形影响进行分析,结果表明:耦合剂同一厚度时,使用机油的探伤回波响应强度大于甘油,使用甘油的回波强度大于水;随着耦合剂厚度呈梯度式变大,目标峰的回波强度随之减小;最后进行实际试验论证理论模型结果。

关键词：计量数值模拟耦合剂工业超声探伤

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Selenoproteins synergistically protect porcine skeletal muscle from oxidative damage via relieving mitochondrial dysfunction and endoplasmic reticulum stress

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畜牧与生物技术杂志:英文版 2023年第5期14卷 2180-2196页

作者： jinzhong Jing Ying He Yan Liu Jiayong Tang Longqiong Wang Gang Jia Guangmang Liu Xiaoling Chen Gang tian Jingyi Cai Lianqiang Che Bo Kang Hua Zhao Key Laboratory for Animal Disease‑Resistance Nutrition of Ministry of Education of China Ministry of Agriculture and Rural Affairsof Sichuan ProvinceAnimal Nutrition InstituteSichuan Agricultural UniversityChengdu 611130SichuanChina College of Animal Science and Technology Sichuan Agricultural UniversityChengdu 611130SichuanChina

Background The skeletal muscle of pigs is vulnerable to oxidative damage,resulting in growth retardation.Selenoproteins are important components of antioxidant systems for animals,which are generally regulated by dietary selenium(Se)level.Here,we developed the dietary oxidative stress(DOS)-inducing pig model to investigate the protective effects of selenoproteins on DOS-induced skeletal muscle growth retardation.Results Dietary oxidative stress caused porcine skeletal muscle oxidative damage and growth retardation,which is accompanied by mitochondrial dysfunction,endoplasmic reticulum(ER)stress,and protein and lipid metabolism disorders.Supplementation with Se(0.3,0.6 or 0.9 mg Se/kg)in form of hydroxy selenomethionine(OH-SeMet)linearly increased muscular Se deposition and exhibited protective effects via regulating the expression of selenotranscriptome and key selenoproteins,which was mainly reflected in lower ROS levels and higher antioxidant capacity in skeletal muscle,and the mitigation of mitochondrial dysfunction and ER stress.What’s more,selenoproteins inhibited DOS induced protein and lipid degradation and improved protein and lipid biosynthesis via regulating AKT/mTOR/S6K1 and AMPK/SREBP-1 signalling pathways in skeletal muscle.However,several parameters such as the activity of GSH-Px and T-SOD,the protein abundance of JNK2,CLPP,SELENOS and SELENOF did not show dose-dependent changes.Notably,several key selenoproteins such as MSRB1,SELENOW,SELENOM,SELENON and SELENOS play the unique roles during this protection.Conclusions Increased expression of selenoproteins by dietary OH-SeMet could synergistically alleviate mitochondrial dysfunction and ER stress,recover protein and lipid biosynthesis,thus alleviate skeletal muscle growth retardation.Our study provides preventive measure for OS-dependent skeletal muscle retardation in livestock husbandry.

关键词： Dietary oxidative stress Endoplasmic reticulum stress Growth retardation Mitochondrial dysfunction Selenoproteins Skeletal muscle

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