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Structural diversity of eukaryotic 18S rRNA and its impact on alignment and phylogenetic reconstruction

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Protein & Cell 2011年第2期2卷 161-170页

作者： Qiang Xie jinzhong lin Yan Qin Jianfu Zhou Wenjun Bu Department of Zoology and Developmental Biology College of Life SciencesNankai UniversityTianjin 300071China National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China College of Information Technical Science Nankai University94 Weijin RoadTianjin 300071China

Ribosomal RNAs are important because they catalyze the synthesis of peptides and proteins.Comparative studies of the secondary structure of 18S rRNA have revealed the basic locations of its many length-conserved and lengthvariable regions.In recent years,many more sequences of 18S rDNA with unusual lengths have been documented in GenBank.These data make it possible to recognize the diversity of the secondary and tertiary structures of 18S rRNAs and to identify the length-conserved parts of 18S rDNAs.The longest 18S rDNA sequences of almost every known eukaryotic phylum were included in this study.We illustrated the bioinformatics-based structure to show that,the regions that are more length-variable,regions that are less length-variable,the splicing sites for introns,and the sites of A-minor interactions are mostly distributed in different parts of the 18S rRNA.Additionally,this study revealed that some length-variable regions or insertion positions could be quite close to the functional part of the 18S rRNA of Foraminifera organisms.The tertiary structure as well as the secondary structure of 18S rRNA can be more diverse than what was previously supposed.Besides revealing how this interesting gene evolves,it can help to remove ambiguity from the alignment of eukaryotic 18S rDNAs and to improve the performance of 18S rDNA in phylogenetic reconstruction.Six nucleotides shared by Archaea and Eukaryota but rarely by Bacteria are also reported here for the first time,which might further support the supposed origin of eukaryote from archaeans.

关键词： secondary structure diversity tertiary structure diversity 18S rRNA Foraminifera Euglenida

基于双金属高核钛氧簇基电极的高性能超级电容器的制备

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无机化学学报 2024年第4期40卷 782-790页

作者：罗稳靳林 Palanisamy Kannan 侯进乐霍鹏姚金忠王鹏周口师范学院河南省稀土功能材料重点实验室周口466001 嘉兴学院生物与化学工程学院嘉兴314001 聊城大学化学化工学院聊城252000 复旦大学航空航天系上海200433 嘉兴学院G60研究院嘉兴314001 西安理工大学仪器科学与技术博士后流动站西安710048

制备了一系列专为超级电容器设计的新型钛氧簇(TOCs,包括Zn-Ti11和Cd-Ti11),扩大了TOCs材料的潜在应用范围。此类材料具有的优异赝电容性能充分展示了钛基材料的优点。所制备的TOCs基超级电容器的最大功率密度为9.5 W·kg^(-1),能量密度... 详细信息

制备了一系列专为超级电容器设计的新型钛氧簇(TOCs,包括Zn-Ti11和Cd-Ti11),扩大了TOCs材料的潜在应用范围。此类材料具有的优异赝电容性能充分展示了钛基材料的优点。所制备的TOCs基超级电容器的最大功率密度为9.5 W·kg^(-1),能量密度为463 Wh·kg^(-1)。

关键词：钛氧簇超级电容器储能苯硫酚

Natural selection on various sites of ribosomal proteins: a cladistic view

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Zoological Systematics 2016年第1期41卷 1-47页

作者： Haoyang Wu Yang Liu Yanhui Wang jinzhong lin Qiang Xie Wenjun Bu Department of Zoology and Developmental Biology College of Life Sciences Nankai University National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences

Deducing the function of certain sites within a protein necessitates a priori recognition of the strength of selective pressure. Currently, statistical method is the only option to evaluate the degree of conservation. In the statistical framework, the types of selective pressure can be divided into classifications of negative, nearly neutral and positive. However, such quantitative methods may omit some crucial amino acid sites among the nearly neutral results. In this study, we propose that the cladistic information can be also important to evaluate the functional importance of various amino acid sites. The ribosomal proteins of 62 eukaryotic species were chosen as the case for statistical and cladistic analysis. The evolutionary changes of each site in the aligned sequences were matched on a currently well-accepted cladogram of eukaryotes. Hundreds of synapomorphic sites were discovered in various clades, in which only part of them were suggested to be potentially significant in the statistical framework. Notably, the mutation on His213 of RPL10 in human beings, which are synapomorphic in vertebrates but only be identified as being under neutral selection, is account for the disease Autism. Therefore, the cladistic information can be complementary to the statistical framework in understanding lineage-specific selection event. Additionally, the bias in the accumulation of apomorphic amino acids is significant when going from the Chordata to the Mammalia lineages. This study emphasizes the value of analyzing transcriptomic and proteomic data in a cladistic way to recognize the presence of group-specific selection on various sites in proteins.

关键词： Autapomorphy synapomorphy functional prediction.

High-elevation Adaptation of Motion Visual Display Modifications in the Toad-Headed Agamid Lizards(Phrynocephalus)

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Asian Herpetological Research 2022年第1期13卷 53-63页

作者： Qiaohan HU Yusong lin Xia QIU jinzhong FU Yin QI Chengdu Institute of Biology Chinese Academy of SciencesChengdu 610041China University of Chinese Academy of Sciences Beijing 101400China Departments of Integrative Biology University of GuelphGuelphOntario N1G 2W1Canada

Understanding the process of adaptation is a key mission in modern evolutionary biology.Animals living at high elevations face challenges in energy meta bolism due to several environmental constraints(e.g., oxygen supply, food availa bility,and movement time). Animal behavioral processes are intimately related to energy meta bolism, and therefore, behavioral modifica tions are expected to be an important mechanism for high-elevation adaptation. We tested this behavioral adaptation hypothesis using va ria tions of motion visual displays in toad-headed agamid lizards of the genus Phr ynocephalus. We predicted tha t complexity of visual motion displays would decrease with the increase of elevation, because motion visual displays are energetically costly. Displays of 12 Phr ynocephalus species were collected with elevations ranging from sea level to 4600 m. We quantified display complexity using the number of display components, display duration, pathways of display components, as well as display speed for each species. Association between display complexity and elevation was analyzed using the phylogenetic generalized least squares(PGLS)model. We found that both the number of display components and the average value of tail coil speed were negatively correlated with elevation, suggesting that toad-headed lizards living at high-elevation areas reduced their display complexity to cope with the environmental constraints. Our research provides direct evidence for high-elevation adaptation from a behavioral aspect and illustrates the potential impacts of environment heterogeneity on motion visual display diversification.

关键词： high-elevation adaptation lizard motion visual display Phrynocephalus signal complexity

基于三级养护标准的沥青路面预养护方案多目标加权灰靶决策

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北京工业大学学报 2024年第5期50卷 591-599页

作者：李海莲司金忠贾卫东付琳李清华杨斯媛张茗兰州交通大学土木工程学院兰州730070 中铁十四局集团有限公司济南250101 中交一公局集团有限公司北京100024

针对沥青路面最佳预防性养护策略选择问题,结合关于沥青路面预防性养护指标及规范标准,建立了基于判断性指标、综合性指标和控制性指标的三级预防性养护判断标准。在此标准下,以多年度项目级养护规划为研究对象,结合GM(1,1)预测模型确... 详细信息

针对沥青路面最佳预防性养护策略选择问题,结合关于沥青路面预防性养护指标及规范标准,建立了基于判断性指标、综合性指标和控制性指标的三级预防性养护判断标准。在此标准下,以多年度项目级养护规划为研究对象,结合GM(1,1)预测模型确定预防性养护时段,并引入多目标智能加权灰靶决策模型对适用性预防性养护方案进行优化决策。在此过程中,确定了养护费用、耐久性等8个养护目标并利用博弈论组合赋权法确定权重,选取G30连霍高速甘肃省境内某路段进行决策分析。结果表明:该路段最早需要开展预防性养护的时间为第3年,在第6年底之后不宜再实施预防性养护,在该预防性养护时段内,方案4的综合效益值最大,其值为0.735 8,即应在第4年采取超薄层罩面预养护措施。该方法简单实用,可成为沥青路面预防性养护策略选择的一种新方法。

关键词：道路工程沥青路面预防性养护预防性养护标准多目标灰靶决策博弈论组合赋权

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护理学博士研究生导师队伍与招生情况分析

中华护理教育 2024年第1期21卷 42-46页

作者：温明月尚少梅贾金忠朱睿林皇涛朱泉熔李粤鑫石河子大学医学院新疆维吾尔自治区石河子市832002 北京大学护理学院北京市100191 北京大学研究生院医学部分院秘书处办公室北京大学公共卫生学院济南市第三人民医院济南市250132

目的了解中国护理学一级学科授权点博士研究生导师队伍结构及招生情况,为护理学科发展提供参考。方法收集并整理2021年24所院校中护理学博士研究生导师信息,分析护理学博士研究生导师数量、结构、分布、招生数量及招生方向。结果24所院... 详细信息

目的了解中国护理学一级学科授权点博士研究生导师队伍结构及招生情况,为护理学科发展提供参考。方法收集并整理2021年24所院校中护理学博士研究生导师信息,分析护理学博士研究生导师数量、结构、分布、招生数量及招生方向。结果24所院校护理学博士研究生导师共计120名,招收108名博士,师均招生不足1人。护理学博士研究生导师以女性为主(77.5%),年龄集中于50~59岁(60.0%),职称以正高级为主(95.0%),最高学历以博士为主(76.7%)。导师分布与招生主要集中于东部地区、双一流院校、综合院校、学科等级为B级的院校,招生集中于25个方向。结论护理学博士点数量少,现有博士导师数量不足,结构不合理,分布不均衡,招生方向不规范。建议通过增加授权点,加强导师队伍建设,合理配置导师资源,优化导师结构,明确护理二级学科方向等措施来改善以上问题。

关键词：护理博士导师招生结构

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石墨消解-原子荧光法同时测定水中砷汞硒

职业与健康 2024年第7期40卷 908-912页

作者：林锦忠张鑫丁友明任智宏黄婵德阳市旌阳区疾病预防控制中心检验科四川德阳618000 德阳市疾病预防控制中心检验科四川德阳618000 成都大学基础医学院四川成都610106

目的建立石墨消解-三通道原子荧光一次样品消解一次进样同时测定水中砷、汞、硒的方法。方法以K2Cr2O7-HNO3-HClO4为消解液,石墨消解仪消解水样,三通道原子荧光同时测定水中砷、汞、硒,标准曲线法定量,测定结果与国标方法进行比较。结... 详细信息

目的建立石墨消解-三通道原子荧光一次样品消解一次进样同时测定水中砷、汞、硒的方法。方法以K2Cr2O7-HNO3-HClO4为消解液,石墨消解仪消解水样,三通道原子荧光同时测定水中砷、汞、硒,标准曲线法定量,测定结果与国标方法进行比较。结果本方法检测水中砷、硒浓度为1.0~20.0μg/L,汞为0.10~2.00μg/L,曲线相关系数均>0.9990;检出限分别为砷0.064μg/L、汞0.030μg/L、硒0.051μg/L,相对标准偏差分别为砷2.95%、汞1.13%、硒1.34%,质控样品测定均值与标准值的相对偏差分别为砷3.37%、汞2.93%、硒-3.39%,加标实验平均回收率分别为砷97.3%、汞99.9%、硒103.3%;配对t检验对2种方法的样品测定结果进行比较,差异均无统计学意义(均P>0.05)。结论所建立的方法能较好的提高工作效率,减少化学试剂用量。方法的曲线线性、检出限、精密度、准确度、样品测定结果均能满足国标方法要求,具有一定的实用价值。

关键词：砷汞硒同时测定原子荧光法石墨消解水质

Canine ACL reconstruction with an injectable hydroxyapatite/collagen paste for accelerated healing of tendon-bone interface

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Bioactive Materials 2023年第2期20卷 1-15页

作者： Qingsong Jiang Liren Wang Zhanhong Liu Jinlei Su Yajun Tang Peijie Tan Xiangdong Zhu Kai Zhang Xing Ma Jia Jiang jinzhong Zhao Hai lin Xingdong Zhang National Engineering Research Center for Biomaterials Sichuan University29 Wangjiang RoadChengduSichuanChina Department of Sports Medicine Shanghai Jiao Tong University Affiliated Sixth People’s Hospital600 Yishan RoadShanghaiChina Department of Orthopaedics First Affiliated Hospital of Xi’an Jiaotong University277 West Yanta RoadXi’anShaanxiChina

Healing of an anterior cruciate ligament(ACL)autologous graft in a bone tunnel occurs through the formation of fibrovascular scar tissue,which is structurally and compositionally inferior to normal fibrocartilaginous insertion and thus may increase the reconstruction failure and the rate of failure recurrence.In this study,an injectable hydroxyapatite/type I collagen(HAp/Col I)paste was developed to construct a suitable local microenvironment to accelerate the healing of bone-tendon interface.Physicochemical characterization demonstrated that the HAp/Col I paste was injectable,uniform and stable.The in vitro cell culture illustrated that the paste could promote MC3T3-E1 cells proliferation and osteogenic expression.The results of a canine ACL reconstruction study showed that the reconstructive ACL had similar texture and color as the native ACL.The average width of the tunnel,total bone volume,bone volume/tissue volume and trabecular number acquired from micro-CT analysis suggested that the healing of tendon-bone interface in experimental group was better than that in control group.The biomechanical test showed the maximal loads in experimental group achieved approximately half of native ACL’s maximal load at 24 weeks.According to histological examination,Sharpey fibers could be observed as early as 12 weeks postoperatively while a typical four-layer transitional structure of insertion site was regenerated at 48 weeks in the experimental group.The injectable HAp/Col I paste provided a biomimetic scaffold and microenvironment for early cell attachment and proliferation,further osteogenic expression and extracellular matrix deposition,and in vivo structural and functional regeneration of the tendon-bone interface.

关键词： Anterior cruciate ligament reconstruction Tendon-bone healing Injectable paste Hydroxyapatite Collagen

A regeneration process-matching scaffold with appropriate dynamic mechanical properties and spatial adaptability for ligament reconstruction

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Bioactive Materials 2022年第7期7卷 82-95页

作者： Xiaojing Xie Junjie Xu Jing lin Jia Jiang Yunfan Huang Jun Lu Yuhao Kang Yage Hu Jiangyu Cai Fujun Wang Tonghe Zhu jinzhong Zhao Lu Wang Key Laboratory of Textile Science&Technology of Ministry of Education College of TextilesDonghua UniversityShanghai201620China Department of Sports Medicine Department of OrthopedicsShanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghai200233China

Ligament regeneration is a complicated process that requires dynamic mechanical properties and allowable space to regulate collagen remodeling.Poor strength and limited space of currently available grafts hinder tissue regeneration,yielding a disappointing success rate in ligament reconstruction.Matching the scaffold retreat rate with the mechanical and spatial properties of the regeneration process remains challenging.Herein,a scaffold matching the regeneration process was designed via regulating the trajectories of fibers with different degradation rates to provide dynamic mechanical properties and spatial adaptability for collagen infiltration.This core-shell structured scaffold exhibited biomimetic fiber orientation,having tri-phasic mechanical behavior and excellent strength.Besides,by the sequential material degradation,the available space of the scaffold increased from day 6 and remained stable on day 24,consistent with the proliferation and deposition phase of the native ligament regeneration process.Furthermore,mature collagen infiltration and increased bone integration in vivo confirmed the promotion of tissue regeneration by the adaptive space,maintaining an excellent failure load of 67.65%of the native ligament at 16 weeks.This study proved the synergistic effects of dynamic strength and adaptive space.The scaffold matching the regeneration process is expected to open new approaches in ligament reconstruction.

关键词： Ligament Regeneration process Fiber Dynamic mechanical property Allowable space