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Discovery of a widespread presence bunyavirus that may have symbiont-like relationships with different species of aphids

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Insect Science 2022年第4期29卷 1120-1134页

作者： Xin An Wei Zhang Chao Ye Guy Smagghe Jin-Jun Wang Jinzhi Niu Key Laboratory of Entomology and Pest Control Engineering College of Plant ProtectionSouthwest UniversityChongqingChina International Joint Laboratory of China–Belgium on Sustainable Crop Pest Control Academy of Agricultural SciencesSouthwest UniversityChongqingChina Department of Plants and Crops Faculty of Bioscience EngineeringGhent UniversityGhentBelgium

Aphids are important agricultural pests,vectors of many plant viruses and have sophisticated relationships with symbiotic microorganisms.Abundant asymptomatic RNA viruses have been reported in aphids due to the application of RNA-seq,but aphid-virus interactions remain unclear.Bunyavirales is the most abundant RNA virus order,which can infect mammals,arthropods,and plants.However,many bunyaviruses have specific hosts,such as insects.Here,we discovered 18 viruses from 10 aphid species by RNA-seq.Importantly,a widespread presence bunyavirus,Aphid bunyavirus 1(ABV-1),was determined to have a wide host range,infecting and replicating in all 10 tested aphid species.ABV-1 may be transmitted horizontally during feeding on plant leaves and vertically through reproduction.In a comparison of the physiological parameters of ABV-1high and ABV-1low strains of pea aphid,higher ABV-1 titers reduced the total nymphal duration and induced the reproduction.Moreover,viral titer significantly affected the lipid and protein contents in pea aphids.In summary,we proposed that ABV-1 may have stable symbiont-like relationships with aphids,and these observations may provide a new direction for studying bunyaviruses in aphids and establishing a model for virus-aphid interactions.

关键词： insec-specific virus pea aphid Phasmaviridae RNA virus RNAi

Insect-specific RNA virus affects the stylet penetration activity of brown citrus aphid(Aphis citricidus)to facilitate its transmission

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Insect Science 2024年第1期31卷 255-270页

作者： Xin An Qiaoying Gu Jing Wang Tengyu Chang Wei Zhang Jin-Jun Wang Jinzhi Niu Key Laboratory of Entomology and Pest Control Engineering College of Plant ProtectionAcademy of Agricultural ScienceSouthwest UniversityChongqingChina

Sap-sucking insects often transmit plant viruses but also carry insect viruses,which infect insects but not plants.The impact of such insect viruses on insect host biology and ecology is largely unknown.Here,we identified a novel insect-specific virus carried by brown citrus aphid(Aphis citricidus),which we tentatively named Aphis citricidus picornavirus(AcPV).Phylogenetic analysis discovered a monophyletic cluster with AcPV and other unassigned viruses,suggesting that these viruses represent a new family in order Picornavirales.Systemic infection with AcPV triggered aphid antiviral immunity mediated by RNA interference,resulting in asymptomatic tolerance.Importantly,we found that AcPV was transmitted horizontally by secretion of the salivary gland into the feeding sites of plants.AcPV influenced aphid stylet behavior during feeding and increased the time required for intercellular penetration,thus promoting its transmission among aphids with plants as an intermediate site.The gene expression results suggested that this mechanism was linked with transcription of salivary protein genes and plant defense hormone signaling.Together,our results show that the horizontal transmission of AcPV in brown citrus aphids evolved in a manner similar to that of the circulative transmission of plant viruses by insect vectors,thus providing a new ecological perspective on the activity of insect-specific viruses found in aphids and improving the understanding of insect virus ecology.

关键词： electrical penetration graph feeding behavior insect virus transmission Picornavirales salivary protein

Characterization of Domeless receptors and the role of Bd Domeless3 in anti-symbiont-like virus defense in Bactrocera dorsalis

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Journal of Integrative Agriculture 2024年第4期23卷 1274-1284页

作者： Wei Zhang Shaoyang Li Rong Li Jinzhi Niu Jinjun Wang Key Laboratory of Entomology and Pest Control Engineering College of Plant ProtectionSouthwest UniversityChongqing 400715China Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River Ministry of Education/International Joint Laboratory of China-Belgium on Sustainable Crop Pest ControlAcademy of Agricultural SciencesSouthwest UniversityChongqing 400715China

The Janus kinase/signal transducers and activators of transcription(JAK/STAT)signaling pathway play a pivotal role in innate immunity.Among invertebrates,Domeless receptors serve as the key upstream regulators of this pathway.In our study on Bactrocera dorsalis,we identified three cytokine receptors:BdDomeless1,BdDomeless2,and BdDomeless3.Each receptor encompasses five fibronectin-type-III-like(FN III)extracellular domains and a transmembrane domain.Furthermore,these receptors exhibit the increased responsiveness to diverse pathogenic challenges.Notably,only BdDomeless3 is upregulated during symbiont-like viral infections.Moreover,silencing BdDomeless3 enhanced the infectivity of Bactrocera dorsalis cripavirus(BdCV)and B.dorsalis picorna-like virus(BdPLV),underscoring BdDomeless3’s crucial role in antiviral defense of B.dorsalis.Following the suppression of Domeless3 expression,six antimicrobial peptide genes displayed decreased expression,potentially correlating with the rise in viral infectivity.To our knowledge,this is the first study identifying cytokine receptors associated with the JAK/STAT pathway in tephritid flies,shedding light on the immune mechanisms of B.dorsalis.

关键词： Bactrocera dorsalis JAK/STAT pathway Domeless receptors antiviral immunity symbiont-like virus

The involvement of systemic RNA interference deficient-1-like (SIL1) in cellular dsRNA uptake in Acyrthosiphon pisum

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Insect Science 2023年第5期30卷 1393-1404页

作者： Chao Ye Xin An Bing-Qin Xie Bi-Yue Ding Jinzhi Niu Jin-Jun Wang Key Laboratory of Entomology and Pest Control Engineering College of Plant ProtectionSouthwest UniversityChongqingChina Academy of Agricultural Sciences Southwest UniversityChongqingChina

Systemic RNA interference deficient-1-like(SIL1)is considered a core component in dsRNA uptake in some insect species.Investigation related to the potential function of SIL1 in dsRNA uptake can contribute to a further understanding of RNA interference(RNAi)mechanisms in insects and agricultural pest control.However,the role of SIL1 in dsRNA uptake in insects such as aphids remains controversial.We have thoroughly analyzed the role of SIL1 from the model aphid Acyrthosiphon pisum(ApSIL1)in cellular dsRNA to clarify its function.First,the induced expression of ApSIL1 upon dsRNA oral exposure provided a vital clue for the possible involvement of ApSIL1 in cellular dsRNA uptake.Subsequent in vivo experiments using the RNAi-of-RNAi approach for ApSIL1 supported our hypothesis that the silencing efficiencies of reporter genes were reduced after inhibition of ApSIL1 expression.The impaired biological phenotypes of aphids,including cumulative average offspring,deformities of the nymph,and mortality upon pathogen infection,were then observed in the treatment group.Thereafter,in vitro dual-luciferase reporter assay showed compelling evidence that the luciferin signal was significantly attenuated when dsluciferase or dsGFP was transferred into ApSIL1-transfected Drosophila S2 cells.These observations further confirmed that the signal of Cy3-labeled dsRNA was rapidly attenuated with time in ApSIL1-transfected Drosophila S2 cells.Overall,these findings conclusively establish that ApSIL1 is involved in dsRNA uptake in A.pisum.

关键词： aphid dsRNA uptake pest control RNAi mechanism SID1

GARP: A family of glycine and alanine-rich proteins that helps spider mites feed on plants

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Insect Science 2023年第5期30卷 1337-1351页

作者： Qin-Zhe Sun Xiao-Lin Li Yu-Fei Shi Yan-Chun Zhang Wen-Jie Chai Ruo-Yu Chen Jinzhi Niu Jin-Jun Wang Key Laboratory of Entomology and Pest Control Engineering College of Plant ProtectionAcademy of Agricultural SciencesSouthwest UniversityChongqingChina

Spider mites(Tetranychidae)are destructive agricultural pests which have evolved strategies to overcome plant defenses,such as the ability to puncture the leaves of their hosts to feed.The expression of many genes with unknown functions is altered during feeding,but little is known about the role of these genes in plant–mite interactions.Here,we identified 3 novel gene families through analysis of genomic and transcriptomic data from 3 spider mite species.These GARP family genes encode glycine and alanine-rich proteins;they are present in mites(Acariformes)but absent in ticks(Parasitiformes)in the subclass Acari,indicating that these genes have undergone a significant expansion in spider mites and thus play important adaptive roles.Transcriptomic analysis revealed that the expression of GARP genes is strongly correlated with feeding and the transfer to new hosts.We used RNA interference to silence GARP1d in the two-spotted spider mite Tetranychus urticae,which inhibited feeding and egg laying and significantly increased mortality when the mites were transferred to soybean shoots;a similar effect was observed after TuVATPase was silenced.However,no changes in mite mortality were observed after TuGARP1d-silenced mites were placed on an artificial diet,which was different from the effect of TuVATPase silencing.Our results indicate that GARP family members play important roles in mite–plant interactions.Additional studies are needed to clarify the mechanisms underlying these interactions.

关键词： feeding mite-plant interaction RNA interference Tetranychus urticae Tetranychus evansi

Delivery of dsRNA for RNAi in insects： an overview and future directions

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Insect Science 2013年第1期20卷 4-14页

作者： Na yu Olivier Christiaens Jisheng Liu Jinzhi Niu Kaat Cappelle Silvia Caccia Hanneke Huvenne Guy Smagghe Department of Crop Protection Faculty of Bioscience Engineering Ghent University Coupure links 653 B-9000 Ghent Belgium

RNA interference （RNAi） refers to the process of exogenous double-stranded RNA （dsRNA） silencing the complementary endogenous messenger RNA. RNAi has been widely used in entomological research for functional genomics in a variety of insects and its potential for RNAi-based pest control has been increasingly emphasized mainly because of its high specificity. This review focuses on the approaches of introducing dsRNA into insect cells or insect bodies to induce effective RNAi. The three most common delivery methods, namely, microinjection, ingestion, and soaking, are illustrated in details and their advantages and limitations are summarized for purpose of feasible RNAi research. In this review, we also briefly introduce the two possible dsRNA uptake machineries, other dsRNA delivery methods and the history of RNAi in entomology. Factors that influence the specificity and efficiency of RNAi such as transfection reagents, selection of dsRNA region, length, and stability of dsRNA in RNAi research are discussed for further studies.

关键词： dsRNA uptake ingestion microinjection pest control RNA interference soaking

Oscillatory Dynamics of Heterogeneous Stem Cell Regeneration

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Communications on Applied Mathematics and Computation 2024年第1期6卷 431-453页

作者： Xiyin Liang jinzhi Lei School of Mathematical Sciences Center for Applied MathematicsTiangong UniversityTianjin300387China

Stem cell regeneration is an essential biological process in the maintenance of tissue homeostasis;dysregulation of stem cell regeneration may result in dynamic diseases that show oscillations in cell numbers.Cell heterogeneity and plasticity are necessary for the dynamic equilibrium of tissue homeostasis;however,how these features may affect the oscillatory dynamics of the stem cell regeneration process remains poorly understood.Here,based on a mathematical model of heterogeneous stem cell regeneration that includes cell heterogeneity and random transition of epigenetic states,we study the conditions to have oscillation solutions through bifurcation analysis and numerical simulations.Our results show various model system dynamics with changes in different parameters associated with kinetic rates,cellular heterogeneity,and plasticity.We show that introducing heterogeneity and plasticity to cells can result in oscillation dynamics,as we have seen in the homogeneous stem cell regeneration system.However,increasing the cell heterogeneity and plasticity intends to maintain tissue homeostasis under certain conditions.The current study is an initiatory investigation of how cell heterogeneity and plasticity may affect stem cell regeneration dynamics,and many questions remain to be further studied both biologically and mathematically.

关键词： Stem cell regeneration Heterogenous Hopf bifurcation Hematopoietic dynamical disease

S掺杂TiO_2/石墨烯复合催化剂制备及光催化分解水制氢

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功能材料 2015年第16期46卷 16125-16128页

作者：魏金枝牛海涛孙晓君张慧哈尔滨理工大学化学与环境工程学院绿色化工技术黑龙江省高校重点实验室哈尔滨150040

为了提高TiO2光解水制氢的光催化性能,通过水热合成法一步制备了S掺杂TiO2/石墨烯(RGO)复合催化剂。采用XRD、XPS、FT-IR、TEM、UV-Vis等测试方法对其进行了表征,并研究了复合催化剂光催化分解水制氢性能。结果表明,在S-TiO2/RGO复合催... 详细信息

为了提高TiO2光解水制氢的光催化性能,通过水热合成法一步制备了S掺杂TiO2/石墨烯(RGO)复合催化剂。采用XRD、XPS、FT-IR、TEM、UV-Vis等测试方法对其进行了表征,并研究了复合催化剂光催化分解水制氢性能。结果表明,在S-TiO2/RGO复合催化剂中,TiO2以14nm左右的纳米颗粒分散在石墨烯片层上;与同等条件下制备的TiO2相比,复合催化剂在可见光区的吸收明显增强;当m(S)∶m(RGO)∶m(TiO2)为1∶0.1∶25时,所制备的复合催化剂在同等条件下产氢速率最大,为141.7μmol/h。

关键词： TiO2 石墨烯 S掺杂光催化制氢

Does the continuous wetting of the Tibetan Plateau contribute to the accelerated degradation of permafrost?

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Science China Earth Sciences 2024年第5期67卷 1714-1718页

作者： Yuyang WANG jinzhi DING Shilong PIAO Sino-French Institute for Earth System Science College of Urban and Environmental SciencesPeking UniversityBeijing 100871China State Key Laboratory of Tibetan Plateau Earth System Environment and ResourcesInstitute of Tibetan Plateau ResearchChinese Academy of SciencesBeijing 100101China

Global warming is causing the Tibetan permafrost to degrade, as evidenced by rising ground temperatures, thicker active layer thickness, thinner permafrost layer, and melting underground ice, etc.(Ran et al., 2018;Wang et al., 2020).The degradation of permafrost not only profoundly alters the carbon cycling processes in high-altitude ecosystems,thereby influencing the regional climate system, but also affects landscape hydrological connectivity。

关键词： Tibetan landscape altitude