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Dynamic Analyses of PrP and PrP^(Sc) in Brain Tissues of Golden Hamsters Infected With Scrapie Strain 263K Revealed Various PrP Forms

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Biomedical and Environmental Sciences 2004年第1期17卷 8-20页

作者： JIAN-MEIGAO CHENGAO JUNHAN XIAO-BOZHOU XIN-LIXIAO jinzhang LANCHEN BAO-YUNZHANG TAOHONG XIAO-PINGDONG[ NationalInstituteforViralDiseaseControlandPrevention ChineseCenterforDiseaseControlandPreventionYingxinRd.100Beijing100052China SchoolofMedicine Xi＇anJiaotongUniversityZhuqueStreet205Xi＇an710061ShaanxiChina InstituteofBasicMedicalScience ChineseAcademyofMedicalSciencesPekingUnionMedicalCollegeDongdanSantiao5Beijing100005China

Objective To expatiate dynamic changes in hamsters infected with scrapie strain 263K, to observe the presence and aggravation of various forms of PrP and PrPSc during incubation period, and to probe primarily the relationship between the onset of clinic manifestations and the presence of different PrPSc forms. Methods Hamster-adapted scrapie strain 263K was intracerebrally inoculated into hamsters. Different forms of PrP and PrPSc were monitored dynamically by Western blot and immuno-histochemical assays. The presence of scrapie-associated fibril (SAF) was assayed by electron microscopy analysis (EM) and immune-golden EM. Results PrPSc was initially detected in the brain tissues of the animals in 20 days post-inoculation by immunohistochemistry and 40 days with Western blot. Quantitative evaluations revealed that the amounts of PrP and PrPSc in brain tissues increased along with the incubation. Several high and low molecular masses of PrP were seen in the brains of the long-life span infected animals. Deglycosylation assays identified that the truncated PrP in the infected brains showed similar glycosylation patterns as the full-length PrP. The presence of short fragments was seemed to relate with the onset of clinical conditions. Conclusion These results indicate that infectious agents exist and accumulate in central nerve system prior to the onset of the illness. Various molecular patterns of PrPSc may indwell in brain tissues during the infection.

关键词： Transmissible spongiform encephalopathies (TSEs) Prion Scrapie Glycosylation Scrapie-associated fibril (SAF)

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Determination of Trace Amount of Yttrium with Bromopyrogallol Red by solid-phase Spectrophotometry

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Rare Metals 2001年第3期20卷 202-204页

作者： jinzhang Gao,Gang Ni, Li Yuan, Jingwan Kang Department of Chemistry, Northwest Normal University, Lanzhou 730070, China 西北师范大学甘肃兰州 730070

A simple and sensitive method for the determination of trace amount of yttrium by solid-phase spectrophotometry has been studied. Yttrium can form a 1 : 1 complex with bromopyrogallol red (BPR) on resin, which was determined directly at 605 nm, pH=6.5. It has a highly sensitivity (epsilon = 6.3 x 10(6)) which is 300-fold higher than the corresponding spectrophotometry in solution. The method was applied to the determination of yttrium in churchite.

关键词： solid-phase spectrophotometry bromopyrogallol red (BPR) yttrium

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Solid-liquid solvent extraction of metal ions

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Journal of University of Science and Technology Beijing 2003年第3期10卷 1-7页

作者： Bo Peng, Haiyan Fan, and jinzhang GaoDepartment of Chemistry, Northwest Normal University, Lanzhou 730070, China NW Normal Univ Dept Chem Lanzhou 730070 Peoples R China

An overview of extraction of some trace metal ions using molten solvent (lowmelting substance) during last two decades is presented. The development of this technique since itsinception is briefly traced. The comparison of extraction efficiency, thermodynamics and kineticsmainly involving extraction of rare earth ions between molten solvent extraction at high temperatureand usual liquid-liquid extraction at room temperature are discussed in detail. The variousparameters obtained from the previous and present studies such as equilibrium extraction constantK_(ex), pH_(1/2), thermodynamic and kinetic data are displayed in tabular form. Finally, the currentdemands, disadvantages and future prospects are also evaluated.

关键词： solid-liquid solvent extraction metal ions thermodynamics kinetics determination

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Investigation of radionuclide～(63)Ni(Ⅱ) sequestration mechanisms on mordenite by batch and EXAFS spectroscopy study

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Science China Chemistry 2012年第4期55卷 632-642页

作者： yang ShiTong,SHENG GuoDong,GUO ZhiQiang,TAN XiaoLi,XU jinzhang & WANG XiangKe Key Laboratory of Novel Thin Film Solar Cells,Institute of Plasma Physics,Chinese Academy of Sciences,Hefei 230031,China Key Laboratory of Novel Thin Film Solar Cells Institute of Plasma Physics Chinese Academy of Sciences Hefei China

The sorption behavior and microscopic sequestration mechanisms of radionuclide 63Ni（II） on mordenite as a function of aging time, ionic srength, initial 63Ni（II） concentrations, solid content and coexistent electrolyte ions were investigated by the combination of batch and EXAFS techniques. Macroscopic experiment results show that the sorption of 63Ni（II） is dependent on ionic strength at pH〈7, and independent of ionic strength at pH〉7. The sorption percentage of 63Ni（II） on mordenite increases with increasing solid content, while the sorption capacity decreases as solid content increases. The presence of different electrolyte ions can enhance or inhibit the sorption of Ni（II） on mordenite in various degrees. EXAFS analysis results of the sam- ples under three different ionic strengths suggest that the retained 63Ni（II） in these samples exists in an octahedral environment with six water ligands. In the initial period of rapid uptake, the sorption of 63Ni（II） is dominated by the formation of innersphere surface complexes. As aging time increases, 63Ni（II） sequestration behavior tends to be mainly controlled by the formation of Ni phyllosilicate coprecipitates and/or Ni（OH）2（s） precipitates. Results for the second shell fit of the sample prepared at an initial 63Ni（II） concentration of 100 mg/L indicate the possible formation of Ni polynuclear surface complexes. Both the macroscopic sorption data and the molecular level evidence of 63Ni（II） surface speciation at the mordenite/water interfaces should be factored into better predictions of the mobility and bioavailability of 63Ni（II） in environment mediums.

关键词： mordenite – 63Ni(II) – aging time – initial concentration – EXAFS analysis

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Geochemical Characteristics of Gases from the Wudalianchi Volcanic Area,Northeastern China

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Acta Geologica Sinica(English Edition) 1999年第2期73卷 225-229页

作者： DU Jianguo LI Shengqiang ZHAO Yi REN jinzhang SUN Rubo DUANMU Heshun Centre for Analysis and Prediction State Seismological Bureau Beijing 100036 Heilongjiang Bureau of Seismology Harbin 150008 Xi'an Institute of Mining Xi'an 710054

The origins of gases in springs, pools and wells from the Wudalianchi (WDLC) volcanic area are discussed based upon molecular and isotope compositions of the gases. Nine gas and water samples were collected from bubbles and water of the springs and pools in the WDLC volcanic area, Northeastern China, in August 1997. The molecular components were measured with a MAT-271 mass spectrometer (MS), helium isotope ratios with a VG-5400 MS, and δ13C with a MAT-251 MS in the Lanzhou Institute of Geology. The gases are enriched in CO2, and most of the CO2 concentrations are over 80% (V). The helium and methane concentrations have relatively wide ranges of 0.7 to 380×10?6 and 4 to 180×10?6, respectively. The 3He/4He ratios are between 1.05 Ra and 3.1 Ra (Ra = 1.4×10?6); the 4He/20Ne values are between 0.45 and 1011, larger than the atmospheric value (0.32). The δ13C (PDB) values of carbon dioxide range from ?9.6 to ?4.2%%. These geochemical data demonstrate that the spring water is from aquifers at different depths, and that helium and carbon dioxide are derived from the mantle, and are contaminated by crust gases during deep fluid migration. Also, there are larger fluxes of deep-earth matter and energy in the WDLC volcanic area.

关键词： gas geochemistry spring volcano Wudalianchi

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Epidemiological and etiological investigation of dengue fever in the Fujian province of China during 2004–2014

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Science China(Life Sciences) 2017年第1期60卷 72-80页

作者： jinzhang Wang Hongbing Chen Meng Huang Yongjun Zhang Jianfeng Xie Yansheng Yan Kuicheng Zheng Yuwei Weng Fujian Center for Disease Control and Prevention Fuzhou 350001 China College of public health Fujian Medicine University Fuzhou 350001 China

Dengue fever(DF) is a vector-borne disease and a tremendous socioeconomic burden on tropical and subtropical countries worldwide. To explore the characteristics of DF epidemic in the Fujian province, information of DF cases in Fujian during 2004–2014 was collected and analyzed. The complete E genes of 48 viral isolates were amplified and sequenced for phylogenetic analysis. A total of 733 cases was reported, of which 612(83.5%) occurred during the peak period from August to October. Additionally, 76%(190/250) of imported cases originated from Southeast Asia countries, by the epidemiological investigation. Phylogenetic analysis of the 48 viral isolates revealed that three genotypes(I, IV, V) of DENV1, and one genotype each of DENV2(cosmopolitan) and DENV3(I) circulated in Fujian during 2004–2014. Similar to the results of the epidemiological investigations, the source of most of the viral isolates, including imported and indigenous cases, may be Southeast Asia countries; however, importation from adjacent provinces was also observed in recent years. Overall, DF is considered an imported epidemic disease in Fujian. Increasing diversity of the viral source and geographic expansion of the area affected by DF in recent years highlights the necessity for strengthening surveillance of the DF epidemic and developing strategies for DF prevention and control in Fujian.

关键词： dengue virus E gene phylogenetic tree epidemiology etiology

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Ushering in Greater Accomplishments

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China Today 2014年第8期63卷 38-39页

作者： LI jinzhang

BRAZIL is a vast land with rich resources. This land of lively people, passionate dancers, mellow coffee, verdant forests and turquoise seas is a gleaming gem of the Southern Hemisphere. Though-separ-ated by vastoceans,the two countries have a long tradition of intercultural rela- tions. Two centuries ago, the first Chinese tea growers reached Brazil after two months at sea. In Brazil they settled the land and began cultivating the seeds of friendship. More Chinese have arrived, helping Brazil- ians build up the country. On August 15, 1974, China and Brazil established formal diplomatic ties and be- gan a new chapter in bilateral relations.

关键词：中国人资源丰富组合模式外交关系巴西南半球蒸发散森林

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Generation of single solitons tunable from 3 to3.8μm in cascaded Er^(3+)-doped and Dy^(3+)-doped fluoride fiber amplifiers

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Photonics Research 2022年第9期10卷 2140-2146页

作者： LINPENG YU JINHUI LIANG SHITING HUANG jinzhang WANG JIACHEN WANG XING LUO PEIGUANG YAN FANLONG DONG XING LIU QITAO LUE CHUNYU GUO SHUANGCHEN RUAN Shenzhen Key Laboratory of Laser Engineering Guangdong Provincial Key Laboratory of Micro/Nano Optomechatronics EngineeringKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong ProvinceCollege of Physics and Optoelectronic EngineeringShenzhen UniversityShenzhen 518060China Key Laboratory of Advanced Optical Precision Manufacturing Technology of Guangdong Higher Education Institutes Shenzhen Technology UniversityShenzhen 518118China Han’s Laser Technology Industry Group Co. Ltd.Shenzhen 518057China

High-power tunable femtosecond mid-infrared(MIR)pulses are of great interest for many scientific and industrial *** we demonstrate a compact fluoride-fiber-based system that generates single solitons tunable from 3 to 3.8μ*** system is composed of an Er:ZBLAN fiber oscillator and amplifier followed by a fusion-spliced Dy:ZBLAN fiber *** Er:ZBLAN fiber amplifier acts as a power booster as well as a frequency shifter to generate Raman solitons up to 3μ*** Dy:ZBLAN fiber amplifier transfers the energy from the residual 2.8μm radiation into the Raman solitons using an in-band pumping scheme,and further extends the wavelength up to 3.8μ*** residual pump radiation and secondary solitons accompanying the soliton self-frequency shift(SSFS)are recycled to amplify Raman solitons,consequently displaying a higher output power and pulse energy,a wider shifting range,and an excellent spectral *** 252 fs pulses at3.8μm with a record average power of 1.6 W and a pulse energy of 23 n J are *** work provides an effective way to develop high-power widely tunable ultrafast single-soliton MIR laser sources,and this method can facilitate the design of other SSFS-based laser systems for single-soliton generation.

关键词： fiber tunable amplifier

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基于AT89C51的新型垃圾处理器设计

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机械管理开发 2024年第8期39卷 100-102,105页

作者：张雨婷章盼梅劳宇鹏刘金璋蔡文鑫林展涛广州城市理工学院广东广州510800

厨余垃圾处理器的刀片及处理流程对垃圾处理效果影响较大,基于此,提出增加4Cr材质的啮齿式双齿辊粉碎结构,并基于AT89C51设计了二次研磨及脱水干燥流程,能有效改善研磨不充分的问题并且实现自动化处理,为家用厨余垃圾处理器的创新设计... 详细信息

厨余垃圾处理器的刀片及处理流程对垃圾处理效果影响较大,基于此,提出增加4Cr材质的啮齿式双齿辊粉碎结构,并基于AT89C51设计了二次研磨及脱水干燥流程,能有效改善研磨不充分的问题并且实现自动化处理,为家用厨余垃圾处理器的创新设计提供了参考。

关键词：厨余垃圾处理技术单片机

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Comparison Study on Clinical and Neuropathological Characteristics of Hamsters Inoculated With Scrapie Strain 263K in Different Challenging Pathways

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Biomedical and Environmental Sciences 2004年第1期17卷 65-78页

作者： jinzhang LANCHEN BAO-YUNZHANG JUNHAN XIN-LIXIAO HAI-YANTIAN BIN-LINGLI CHENGAO JIAN-MEIGAO GUI-PINGMA CAI-MINXU YONGLIU XIAO-PINGDONG NationalInstituteforViralDiseaseControlandPrevention ChineseCenterforDiseaseControlandPreventionYingxinRd.100Beijing100052China NationalLaboratoryofMedicalMolecularBiology InstituteofBasicMedicalScienceChineseAcademyofMedicalSciencesandPekingUnionMedicalCollegeDongDanSanTiao5Beijing100005China SchoolofMedicine Xi＇anJiao-TongUniversityZhuqueStreet205Xi＇an710061ShaanxiChina BSETestLaboratory BeijingEntry-ExitInspectionandQuarantineBureauBeijing100029China SchoolofMedicine xi＇anJiao-TongUniversityZhuqueStreet205Xi＇an710061

Objective To understand the infectious characteristics of a hamster-adapted scrapie strain 263K with five different routes of infection including intracerebral (i.e.), intraperitoneal (i.p.), intragastrical (i.g.), intracardiac and intramuscular (i.m.) approaches. Methods Hamsters were infected with crude- or fine-prepared brain extracts. The neuropathological changes, PrPSc deposits, and patterns of PK-resistant PrP were analyzed by HE stain, immunohistochemistry (IHC) assay and Western blot. Reactive gliosis and neuron loss were evaluated by glial fibrillary acidic protein (GFAP) and neuron specific enolase (NSE) specific IHC. Results The animals inoculated in i.m. and Lp. ways with crude PrPSc extracts showed clinical signs at the average incubation of 69.212.8 and 65.5±3.9 days. Inoculation in i.c. and intracardiac ways with fine PrPSc extracts (0.00035 g) caused similar, but relative long incubation of around 90 days. Only one out of eight hamsters challenged in i.g way with low dosage (0.01 g) became ill after a much longer incubation (185 d), while all animals (4/4) with high dosage (0.04 g) developed clinical signs 105 days postinfection. The most remarkable spongiform degeneration and PrPSc deposits were found in brain stem among the five challenge groups generally. The number of GFAP-positive astrocytes increased distinctly in brain stems in all infection groups, while the number of NSE-positive cells decreased significantly in cerebrum, except i.c. group. The patterns of PK-resistant PrP in brains were basically identical among the five infection routes. Conclusion Typical TSE could be induced in hamsters by inoculating strain 263K in the five infection ways. The incubation periods in bioassays depend on infective dosage, administrating pathway and preparation of PrPSc. The neuropathological changes and PrPSc deposits seem to be related with regions and inoculating pathways.

关键词： Transmissible spongifonn encephalopathy Scrapie Strain 263K PrP^(Sc) Bioassay Neuropathology Inoculating pathways

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