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Expression patterns of the rice class I metallothionein gene family in response to lead stress in rice seedlings and functional complementation of its members in lead-sensitive yeast cells

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Chinese Science Bulletin 2007年第16期52卷 2203-2209页

作者： XU YuFeng ZHOU GongKe ZHOU Lu LI YiQin liu jinyuan Laboratory of Molecular Biology and Protein Science Key Laboratory of the Ministry of Education Department of Biological Sciencesand Biotechnology Tsinghua University Beijing 100084 China

Metallothioneins (MTs) are a group of low molecular mass and cysteine-rich proteins that can chelate heavy-metal ions. In this paper,Northern blot analysis was used to investigate the influence of lead stress on the expression patterns of 10 rice class I MT genes (OsMT-Is) in rice seedlings. With the ex-ception of OsMT-I-3b,the data demonstrate dynamic changes of 9 OsMT-I transcripts in response to Pb2+ treatment in rice seedling roots. Of these genes,transcription of OsMT-I-1a,OsMT-I-1b,OsMT-I-2c,OsMT-I-4a,OsMT-I-4b and OsMT-I-4c increased significantly,while transcription of OsMT-I-2a and OsMT-I-3a increased marginally. In contrast,the expression of OsMT-I-2b was inhibited. Pb2+ induced the expression of 6 OsMT-I genes in seedling shoots,but had no obvious effects on the expression of OsMT-I-1a,OsMT-I-1b,OsMT-I-4a and OsMT-I-4b. All the 10 OsMT-Is had enhanced lead tolerance when heterologously expressed in lead-sensitive yeast mutant cells. These results provide an expression profile of the rice MT gene family in response to Pb2+ stress in rice seedlings and demonstrate in-creased lead tolerance in sensitive yeast mutant cells expressing OsMT-Is. This study lays a foundation for further analysis of the role of the rice MT gene family in respond to Pb2+ stress.

关键词：水稻金属硫蛋白基因表达铅酵母细胞

Molecular architecture and engineering of spider dragline silk protein

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Progress in Natural Science:Materials International 2005年第9期15卷 769-776页

作者： ZHANG Hengmu and liu jinyuan(Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China) Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

Spider dragline silk, which is produced in spider major ampullate gland, is a composite proteinacious fiber with highly repetitive Ala-Gly-rich domain. The unique combination of both high tensile strength and high elasticity makes spider dragline silk superior to almost any other natural or synthetic fibers. Cloning of the genes reveals that the silk is composed of at least two major proteins. Each protein component contains multiple repeats of modular structures that alternate between Ala-rich domains and Gly-rich domains. Molecular engineering not only opens a door to the production of spidroins but also provides a valuable experimental system to test and further establish the relationship between modular structures and mechanical properties. Here, based on our own studies, we review the latest progress of the modular structure and genetic engineering and outline the future prospects.

关键词： spider dragline silk protein modular structure molecular engineering.

A Visualized Fusion Protein Based on Self-assembly Hydrophobin HGFI

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Chemical Research in Chinese Universities 2015年第5期31卷 781-786页

作者： ZHAO Liqiang liu jinyuan SONG Dongmin WANG Xiangxiang TAI Feifei XU Haijin QIAO Mingqiang Key Laboratory of Bioaetive Materials Ministry of Education College of Life Sciences Nankai University Tianjin 300071 P. R. China

Hydrophobins are a type be used to modify material surfaces of small amphipathic proteins with a and adsorb enzymes, antibodies and unique self-assembly property, which can even cells. In this study, a fusion protein consisting of hydrophobin HGFI and green fluorescent protein（GFP） was successfully obtained from Pichia patoris （P. pastoris）. Water contact angle（WCA） measurement proves that the wettability of the surfaces of different materials was changed. We further demonstrated the self-assembly ability of HGFI-GFP, which can be used to disperse the multi-walled carbon nanotubes（MWCNTs）. Finally, the adsorption of HGFI-GFP onto the surface of the tissue engineering material poly（ε-caprolactone）（PCL） was evaluated by detecting the fluorescence of the fusion protein itself. The resalt demonstrates that both the basic self-assembly activity of the HGFI domain and the functional activity of the GFP domain were still remained.

关键词： Self-assembly Hydrophobin Fusion protein Dispersion Modification

Subwavelength on-chip light focusing with bigradient all-dielectric metamaterials for dense photonic integration

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InfoMat 2022年第2期4卷 139-151页

作者： Zhihao Ren Bowei Dong Qifeng Qiao Xinmiao liu jinyuan liu Guangya Zhou Chengkuo Lee Department of Electrical and Computer Engineering National University of SingaporeSingaporeSingapore Center for Intelligent Sensors and MEMS(CISM) National University of SingaporeSingaporeSingapore NUS Suzhou Research Institute(NUSRI) SuzhouChina Department of Mechanical Engineering National University of SingaporeSingaporeSingapore NUS Graduate School-Integrative Sciences and Engineering Programme(ISEP) National University of SingaporeSingaporeSingapore

Photonic integrated circuits(PICs)provide a promising platform for miniaturized on-chip optical systems for communication,computation,and sensing *** dense integration of photonic components is one of the keys to exploit the advantages of *** light focusing is a fundamental and indispensable function in PICs,focusing light at the micro/nanometer-scale is ***,a bigradient on-chip metalens(BOML)is proposed to achieve ultrasmall focal lengths and spot sizes at the subwavelength scale for dense *** design of BOML combines gradient geometry and gradient refractive index into one metalens by simultaneously engineering the length and width of subwavelength silicon *** a small device footprint of only 168μm,the BOML achieves efficient on-chip focusing with the recordbreaking figure-of-merits,which are the ratio of wavelength to focal length/spot size(0.268 and 2.83)and numerical aperture(1.78).Leveraging on the Fresnel design,the footprint of BOML is further reduced by 55.1%,and the numerical aperture is enhanced to *** demonstration of mode conversion and beam steering with efficiency over 80%and a tilting range of 7.2°holds the potential for highly dense on-chip photonic systems for optical communication,optical sensing,nonlinear optics,and neural networks for deep learning.

关键词： all-dielectric metamaterials beam steering mid-infrared on-chip focusing photonic integrated circuits

On the Systematic Position of Eosipterus yangi Ji et Ji,1997 among Pterodactyloids

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Acta Geologica Sinica(English Edition) 2006年第5期80卷 643-646页

作者： LU Junchang GAO Chunling MENG Qingjin liu jinyuan JI Qiang Institute of Geology Chinese Academy of Geological Sciences Beijing 100037 Dalian Natural History Museum Dalian Liaoning 116023

A comparison of the new specimen with the holotype of Eosipterus from western Liaoning Province indicates that Eosipterus is a valid genus, which belongs to the family Ctenochasmatidae. The revised diagnosis of Eosipterus includes the wing span is less than 1.25 meters, the length of the ulna is nearly equal to these of wing phalanx 2 and the tibia, humerus is longer than tibia, and the ratio of metatarsal to tibia is approximately 0.40.

关键词： Eosipterus systematic position Yixian Formation western Liaoning

Enhancement of the Photocatalytic Oxygen Production by Tantalum Nitride Supported Fe Atom Embedded N-Doped Graphene Oxide

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Chinese Journal of Chemistry 2023年第3期41卷 280-286页

作者： Hanxiang Chen Jia Yan Zhao Mo jinyuan liu Xianglin Zhu Huaming Li Hui Xu School of the Environment and Safety Engineering School of the Institute for Energy Researchand School of the Materials Science&EngineeringJiangsu UniversityZhenjiangJiangsu212013 China

Energy crises and environmental pollution have become urgent problems with human civilization *** technology is a green method to deal with these *** key to improve photocatalytic efficiency lies in the effective separation of photogenerated electron-hole *** this work,we designed the Fe atom embedded N-doped graphene oxide(Fe-NGO)supporting on tantalum nitride(Ta_(3)N_(5))catalyst,which was employed to improve the photocatalytic oxygen production *** oxygen production of 5 wt%Fe atom embedded N-doped graphene oxide supporting on tantalum nitride(Fe-NGO/Ta_(3)N_(5))was 184.7μmol·g^(-1),about 3.5 times higher than that of the pure Ta_(3)N_(5).The introduction of the cocatalyst Fe-NGO acting as an electron conductor in the Fe-NGO/Ta_(3)N_(5) accelerates the carrier migration of Ta_(3)N_(5) and further enhances the photocatalytic oxygen production activity.N-doping increases the conductivity of graphene oxide(GO),and Fe atoms are used as the reactive sites to promote the combination of electron and sacrificial agent in the *** work may provide insights into the research of new carbon.

关键词： Photocatalysis Electron transfer Fe atom embedded N-doped graphene oxide Ta_(3)N_(5) Catalysis

Predictive values of multidetector-row computed tomography combined with serum tumor biomarkers in preoperative lymph node metastasis of gastric cancer

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Chinese Journal of Cancer Research 2019年第3期31卷 453-462页

作者： Huihui Bai Jingyu Deng Nannan Zhang Huifang liu Wenting He jinyuan liu Han Liang Department of Gastric Cancer Tianjin Medical University Cancer Institute & Hospital National Clinical Research Center for Cancer Key Laboratory of Cancer Prevention and Therapy Tianjin’s Clinical Research Center for Cancer

Objective: Multidetector-row computed tomography(MDCT) and serum tumor biomarkers are commonly used to evaluate the preoperative lymph node metastasis and the clinical staging of gastric cancer(GC). This study intends to evaluate the clinical predictive value of MDCT and serum tumor biomarkers in lymph node metastasis of ***: The clinicopathologic data of 445 GC patients who underwent radical gastrectomy were retrospectively analyzed to evaluate the diagnostic value of MDCT and serum tumor biomarkers in lymph node metastatic staging of GC before ***: With the multinomial logistic regression analysis, the independent relative factors of lymph node metastasis of GC were identified as tumor size, depth of tumor invasion, vessel invasion, vascular embolus, and soft tissue invasion. The optimal critical value of the short diameter of lymph nodes detected by MDCT scanning for evaluation of preoperative lymph node metastasis was 6.0 mm, with 75.7% as predictive accuracy of lymph node metastasis compared to the postoperative pathological results of GC patients. In addition, the critical value of the short diameter of lymph nodes combined with serum tumor biomarkers [including carbohydrate antigen(CA)-724 and CA-199] could show an enhancement of predictive sensitivity of lymph node metastasis(up to 89.3%) before ***: MDCT combined with serum tumor biomarkers should be adopted to improve preoperative sensitivity and accuracy of lymph node metastasis for GC patients.

关键词： Gastric cancer MDCT serum tumor biomarkers preoperative

Heterogeneous hybrid immunity against Omicron variant JN.1 at 11 months following breakthrough infection

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Signal Transduction and Targeted Therapy 2024年第8期9卷 3170-3172页

作者： Xuan He Jingyou Yu Jiajing Jiang jinyuan liu Qi Qi Dan liu Weimin Li Department of Pulmonary and Critical Care Medicine Precision Medicine Key Laboratory of Sichuan ProvinceState Key Laboratory of Respiratory Health and MultimorbidityWest China HospitalSichuan UniversityChengduSichuanChina Guangzhou National Laboratory Bio-lslandGuangzhouGuangdongChina Sichuan Center for Disease Control and Prevention ChengduSichuanChina

Dear Editor,Due to the ongoing evolution of SARS-CoV-2 and waning immunity following COVID-19 vaccination,breakthrough infections have become common *** exposure to viral antigens during different COVID-19 waves and vaccination contribute to the unique"hybrid immunity"-immune protection conferred by the combined vaccination and natural infection in individuals.

关键词： immunity vaccination infection

Dongbeititan dongi,the First Sauropod Dinosaur from the Lower Cretaceous Jehol Group of Western Liaoning Province,China

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Acta Geologica Sinica(English Edition) 2007年第6期81卷 911-916页

作者： WANG Xuri YOU Hailu MENG Qingjin GAO Chunling CHENG Xiaodong liu jinyuan Dalian Natural History Museum Dalian Liaoning Province China Institute of Geology Chinese Academy of Geological Sciences Beijing 100037 China

We herein describe a partial postcranial skeleton of a sauropod dinosaur recovered from the Lower Cretaceous Jehol Group in the Beipiao area of western Liaoning Province, northeastern China. A suite of features it possesses, including the camellate internal structure of its presacral elements, the existence of pneumatocoels on the proximal ends of the dorsal ribs, and especially the medially deflected proximal portion of the femur, definitively establish the titanosauriform affinities of the specimen. It differs from other titanosauriforms in having a craniocaudally elongate coracoid with a squared cranioventral extreme and a long, smooth, and slightly convex acetabular edge of the pubis. It represents a new taxon, Dongbeititan dongi gen. et sp. nov. Comparative studies suggest that Dongbeititan is a basal titanosauriform, more derived than Euhelopus, Fusuisaurus, and Huanghetitan, but less derived than Gobititan and Jiutaisaurus. Dongbeititan represents the first sauropod dinosaur reported from the Lower Cretaceous Jehol Group of western Liaoning Province.

关键词： Dinosauria Sauropoda Titanosauriformes Jehol Group Yixian Formation Lower Cretaceous Beipiao western Liaoning Province China