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The Arabidopsis Spontaneous Cell Death1 gene, encoding a ζ-carotene desaturase essential for carotenoid biosynthesis, is involved in chloroplast development, photoprotection and retrograde signalling

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Cell Research 2007年第6期17卷 458-470页

作者： Haili Dong1,2 Yan Deng1,2 jinye mu1 Qingtao Lu3 Yiqin Wang1 Yunyuan Xu3 Chengcai Chu1 Kang Chong3 Congming Lu3 Jianru Zuo1 1 State Key Laboratory of Plant Genomics and National Plant Gene Research Center (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beoing 100101, China 2 Graduate School, Chinese Academy of Sciences, Beijing 100039, China 3 Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China InstituteofGeneticsandDevelopmentalBiologyChineseAcademyofSciencesBeijing100101China GraduateSchoolChineseAcademyofSciences Beijing

Carotenoids, a class of natural pigments found in all photosynthetic organisms, are involved in a variety of physiologi-cal processes, including coloration, photoprotection, biosynthesis of abscisic acid (ABA) and chloroplast *** carotenoid biosynthesis has been well studied biochemically, the genetic basis of the pathway is not wellunderstood. Here, we report the characterization of two allelic Arabidopsis mutants, spontaneous cell death 1-1 (spcl-1)and spcl-2. The weak allele spcl-1 mutant showed characteristics of bleached leaves, accumulation of superoxide andmosaic cell death. The strong mutant allele spcl-2 caused a complete arrest of plant growth and development shortlyafter germination, leading to a seedling-lethal phenotype. Genetic and molecular analyses indicated that SPC1 encodesa putative ζ-carotene desaturase (ZDS) in the carotenoid biosynthesis pathway. Analysis of carotenoids revealed thatseveral major carotenoid compounds downstream of SPC1/ZDS were substantially reduced in spcl-1, suggesting thatSPC1 is a functional ZDS. Consistent with the downregulated expression of CAO and PORB, the chlorophyll contentwas decreased in spcl-1 plants. In addition, expression of Lhcb1.1, Lhcb1.4 and RbcS was absent in spcl-2, suggestingthe possible involvement of carotenoids in the plastid-to-nucleus retrograde signaling. The spcl-1 mutant also displaysan ABA-deficient phenotype that can be partially rescued by the externally supplied phytohormone. These results suggestthat SPC1/ZDS is essential for biosynthesis of carotenoids and plays a crucial role in plant growth and development.

关键词： carotenoid biosynthesis cell death chloroplast development photoprotection Arabidopsis SPC1/ZDS

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Arabidopsis Transcription Factor Genes NF-YA 1, 5, 6, and 9 Play Redundant Roles in Male Gametogenesis, Embryogenesis, and Seed Development

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Molecular Plant 2013年第1期6卷 188-201页

作者： jinye mu Helin Tan Sulei Hong Yan Liang Jianru Zuo State Key Laboratory of Plant Genomics and National Plant Gene Research Center (Beijing) Institute of Genetics and Developmental Biology ChineseAcademy of Sciences Datun Road Beijing 100101 China Graduate School Chinese Academy of Sciences Beijing 100049 China

Nuclear factor Y （NF-Y） is a highly conserved transcription factor presented in all eukaryotic organisms, and is a heterotrimer consisting of three subunits： NF-YA, NF-YB, and NF-YC. In Arabidopsis, these three subunits are encoded by multigene families. The best-studied member of the NF-Y transcription factors is LEAFY COTYLEDON1 （LEC1）, a NF-YB family member, which plays a critical role in embryogenesis and seed maturation. However, the function of most NF-Y genes remains elusive. Here, we report the characterization of four genes in the NF-YA family. We found that a gain- of-function mutant of NF-YA1 showed defects in male gametogenesis and embryogenesis. Consistently, overexpression of NF-YA1, 5, 6, and 9 affects male gametogenesis, embryogenesis, seed morphology, and seed germination, with a stronger phenotype when overexpressing NF-YA1 and NF-YA9. Moreover, overexpression of these NF-YA genes also causes hypersensitivity to abscisic acid （ABA） during seed germination, retarded seedling growth, and late flowering at different degrees. Intriguingly, overexpression of NF-YA1, 5, 6, and 9 is sufficient to induce the formation of somatic embryos from the vegetative tissues. However, single or double mutants of these NF-YA genes do not have detectable phenotype. Collectively, these results provide evidence that NF-YA1, 5, 6, and 9 play redundant roles in male gameto- phyte development, embryogenesis, seed development, and post-germinative growth.

关键词： Arabidopsis embryogenesis male gametogenesis nuclear factor YA seed development.

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Breeding of *** translocation lines with *** cytoplasm and characterization by GISH

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Chinese Science Bulletin 2000年第15期45卷 1404-1408,1443页

作者： mu jinye LI Jilin WANG Xianping JING Jiankang ZHANG Xiangqi Institute of Genetics Chinese Academy of SciencesBeijing 100101China Department of Biology Harbin Normal UniversityHarbin 150080China

A male-sterile T. aestivum-Ag. intermedium partial amphiploid with cytoplasm of T. timopheevii as a female parent was crossed to common wheat. The hybrid was backcrossed to the male parent several times continually and self-crossed at last. Two stable lines with common wheat phenotype, H96269-2 and H96278, have been obtained. The chromosome numbers of the two lines are all 2n = 42 in somatic cells. By inoculation test, the two lines show a high level of resistance to yellow rust. Through genomic in situ hybridization (GISH) with Ag. intermedium total genomic DNA as a probe, it is demonstrated that the two stable lines are all small segmental translocation lines, and the translocated chromosome segments from Ag. intermedium are located on the short arm terminals of wheat chromosomes. Genetics analysis suggests that the yellow rust resistance gene(s) are probably located on the translocated chromosome segments of Ag. intermedium.

关键词： T. aestivum-Ag. intermedium translocation line ailoplasmic line genomic in situ hybridization yellow rust.

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STRENGTH DESIGN OF PREMIUM THREADED CASING CONNECTION

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Chinese Journal of Mechanical Engineering 2004年第1期17卷 110-113页

作者： GaoLianxin jinye ZhangYi SchoolofMechanicalEngineering ShanghaiJiaotongUniversityShanghai200030China SteelTubeBranchCo ShanghaiBaosteelGroupCorporationShanghai201900China

Using premium casing connections instead of API ones is one of the mosteffective technique to prevent casing failure. The factors contribute to the strength of premiumcasing connections are studied with FEA and full-scale test. The criterions are presented thatensure the connection's strength higher than the pipe. At the same time, the method is given todecrease the peak stress of the connection so as to improve its anticorruption property. At last,full-scale tests are done to test the strength of the connections designed with the methoddescribed, the results show that the connection's strength is higher than the pipe. This indicatedthat the method described is effective in designing premium casing connection.

关键词： Casing connection Premium thread Connecting strength Finite elementmethod Full-scale test

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Accuracy for superposition of squeezed states in lossless and dissipative channel

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Science China(Physics,Mechanics & Astronomy) 2013年第3期56卷 593-599页

作者： XIE Duan PENG jinye School of Electronics and Information Northwestern Polytechnical University School of Electronic Engineering Xi'an University of Posts and Telecommunications

The analysis of accuracy for superposition of squeezed states(SSSs) in lossless and loss case has been performed in this *** lossless case,time accuracies of SSSs with mean photon number n s have a scaling of ns-2 in two limits of large and small *** the help of photon loss model,the dissipative channel will degrade accuracies has been *** the limit of large squeezing,the accuracy will slowly decrease with the reduction of *** the limit of small squeezing,time accuracy scales as 1/(η4ns2) and will decrease much faster along with decreases.

关键词： time accuracy quantum clock synchronization superposition of squeezed states

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Continuous-Variable Quantum Key Distribution Scheme with Odd Coherent States

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Chinese Journal of Electronics 2018年第2期27卷 433-438页

作者： DONG Yingdi PENG jinye SUN Xiaoyan School of Electronics and Information Northwestern Polytechnical University School of Information and Control Engineering Xi'an University of Architecture and Technology

The Quantum key distribution(QKD) has been entering the practical application era. The odd coherent state which follows sub Poisson distributed has been confirmed to extend transmission distance and improve secret key rate in Discrete-variable quantum key distribution(DVQKD) protocol. We propose Entanglement-based(EB)scheme of Continuous-variable quantum key distribution(CVQKD) with odd coherent state. This scheme allows distributing Gaussian modulated CVQKD with laser light which could be odd coherent state. We analyze the logarithmic negativity between entangled odd coherent states and the security. The results show that renewed scheme can extend secure distance by 10.6% and enhance the tolerance excess noise in same parameter, and the renewed scheme could be applied to prepare-and-measure(P&M)model in CVQKD.

关键词： Quantum key distribution(QKD) Odd coherent state Entanglement-based(EB) Sub Poisson distributed

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A rigorous real-time acoustic positioning method for ocean bottom seismic exploration

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Satellite Navigation 2024年第1期5卷 168-190页

作者： jinye Ma Shouchuan Fang Jianhu Zhao School of Geodesy and Geomatics Wuhan University129 Luoyu RoadWuhan430079China Institute of Marine Science and Technology Wuhan UniversityWuhan430079China Surveying Service Center BGP Inc.CNPCZhuozhou072751HebeiChina

The conventional technique for positioning seafloor geophones in ocean bottom seismic exploration encounters several challenges,including the significant impact of outliers on positioning results,underutilization of high-precision observations,and low efficiency in real-time data *** issues inevitably affect the quality of seismic exploration *** address these challenges and enhance the accuracy of geophone positioning,this paper proposes a rigorous real-time acoustic positioning method for geophones based on sequential adjustment and Baarda's outlier detection *** proposed method comprises three key steps:grouping the original acoustic observations,constructing the intra-group acoustic positioning model,and synthesizing the positioning results across the different *** validity and practicality of this approach are confirmed through a simulation experiment as well as the field experiment conducted in the Bohai Sea,*** results demonstrate that the proposed method effectively eliminates outliers in the original observations and maximizes the utilization of high-quality *** to traditional acoustic positioning methods,it significantly reduces positioning errors from meters to decimeters,and in some cases can achieve centimeter-level *** the sound velocity profile in the operating sea area is measured,the method can attain the posterior standard deviation at the millimeter level and positioning errors within 10 *** the sound velocity profile is unknown,the method can achieve the posterior standard deviation at centimeter-level and positioning errors of approximately 20 cm.

关键词： Ocean bottom seismic exploration Geophone positioning Acoustic positioning method Sequential adjustment Outlier detection

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MODEL OF OPERATING AND BALANCING ASSEMBLY LINE IN A MASS CUSTOMIZATION ENVIRONMENT

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Chinese Journal of Mechanical Engineering 2003年第4期16卷 344-347页

作者： WeiDong jinye WangRong WangZheng SchoolofMechanicalEngineenng ShanghaiJiaotongUniversityShanghai200030China SchoolofManagement ShanghaiJiaotongUniversityShanghai200030China SchoolofMechanicalEngineering ShanghaiJiaotongUniversityShanghai200030China

The successful implementation of mass customization lies on reengineeringtechnology and management methods to organize the production. Especially in assembly phase, variousproduct configurations, due-time penalties and order-driven strategy challenge the traditionaloperation and management of assembly lines. The business features and the operation pattern ofassembly line based on mass customization are analyzed. And the research emphatically studiesvarious technologic factors to improve customer satisfaction and their corresponding implementmethods in operating assembly line. In addition, the models are proposed for operating assembly lineunder dynamic process environment in mass customization. A genetic approach is developed to providethe optimal solution to the models. The effectiveness of the proposed approach is evaluated with anindustrial application.

关键词： Mass customization Assembly line Balancing Optimal model

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Effects of nitrogen and phosphorus concentrations on the bioaccumulation of polybrominated diphenyl ethers by Prorocentrum donghaiense

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Journal of Environmental Sciences 2013年第2期25卷 376-385页

作者： Chao Chai Xundong Yin Wei Ge jinye Wang College of Resources and Environment Qingdao Agricultural University College of Life Sciences Qingdao Agricultural University College of Animal Science and Veterinary Qingdao Agricultural University

The growth, cellular total lipids, bioaccumulation amount, and bioaccumulation factors （BAFs） of 2,4,4 ′ -tribromodiphenyl ether （BDE28）, 2,2 ′ ,4,4 ′ -tetrabromodiphenyl ether （BDE47）, and 2,2 ′ ,4,4 ′ ,5-pentabromodiphenyl ether （BDE99） in a semi-continuous culture of Prorocentrum donghaiense were studied in relation to nitrate （0, 128, and 512 μmol/L） and phosphate （0, 8, and 32 μmol/L） concentrations. The BDE28, BDE47, and BDE99 content per cell under 0 μmol N/L were 3.77 × 10 6 , 3.95 × 10 6 , and 4.32 × 10 6 ng/cell, respectively, which were significantly higher than those under 128 and 512 μmol N/L. A nearly 5-fold increase in polybrominated diphenyl ether （PBDE） content per algal cell was found between 0 and 8 μmol P/L and between 8 and 32 μmol P/L. With increasing N and P concentrations, the PBDE content per volume of algal culture and the accumulation percentage of available PBDEs declined slightly. The BAFs for the PBDEs based on lipids showed that the logBAF lip under 0 μmol N/L was higher than those under 128 and 512 μmol N/L. The logBAF lip under 0 μmol P/L was higher than that under 8 μmol P/L but lower than that under 32 μmol P/L. Correlation analysis indicated a significant negative correlation between nutrient concentration and cellular total lipids, as well as the PBDE content per cell. The results indicate that different N and P concentrations change the total lipids content of P. donghaiense, thereby resulting in varying PBDE accumulation.

关键词： nitrogen phosphorus bioaccumulation polybrominated diphenyl ethers Prorocentrum donghaiense

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A novel small-signal equivalent circuit model for GaN HEMTs incorporating a dual-field-plate

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Journal of Semiconductors 2024年第5期45卷 61-68页

作者： jinye Wang Jun Liu Zhenxin Zhao School of Integrated Circuit Science and Engineering Hangzhou Dianzi UniversityHangzhou 310018China

An accurate and novel small-signal equivalent circuit model for GaN high-electron-mobility transistors(HEMTs)is proposed,which considers a dual-field-plate(FP)made up of a gate-FP and a *** equivalent circuit of the overall model is composed of parasitic elements,intrinsic transistors,gate-FP,and source-FP *** equivalent circuit of the gate-FP is identical to that of the intrinsic *** order to simplify the complexity of the model,a series combination of a resistor and a capacitor is employed to represent the *** analytical extraction procedure of the model parameters is presented based on the proposed equivalent *** verification is carried out on a 4×250μm GaN HEMT device with a gate-FP and a source-FP in a 0.45μm *** with the classic model,the proposed novel small-signal model shows closer agreement with measured S-parameters in the range of 1.0 to 18.0 GHz.

关键词： small-signal model dual field-plate(FP) GaN high-electron-mobility transistors(HEMT) parameter extraction

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