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An in planta haploid induction system in Brassica napus

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Journal of Integrative Plant Biology 2022年第6期64卷 1140-1144页

作者： Yifan Li Dan Li Qing Xiao Huadong Wang Jing Wen Jinxing Tu jinxiong shen Tingdong Fu Bin Yi National Key Laboratory of Crop Genetic Improvement National Center of Rapeseed ImprovementHuazhong Agricultural UniversityWuhan 430070China Key Laboratory of Crop Physiology Ecology and Genetic BreedingMinistry of EducationJiangxi Agricultural UniversityNanchang 330045China

Doubled haploid technology is widely used to accelerate plant breeding,but its use in the important oilseed crop Brassica napus *** limited because *** haploids could only be obtained through in vitro anther or microspore ***,maize(Zea mays)lines containing mutations in Domain of unknown function 679 membrane protein(DMP)were used as haploid inducer *** new haploid induction mechanism has been extended to several other plants,including the dicots Arabidopsis thaliana,tomato(Solanum lycopersicum),and tobacco(Nicotiana tabacum).Here,we knocked out four BnaDMP genes in the *** cultivar Westar using a clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9 vector with an enhanced green fluorescent protein expression *** with DMP mutations in *** in the T_(0),T_(1),and T_(2) generations exhibited a haploid induction rate up to 2.53%.These results suggest that targeting BnaDMP could be useful for haploid induction in ***.

关键词： Brassica napus CRISPR/Cas9 fluorescence screening maternal haploid induction

Genome-wide prediction for hybrids between parents with distinguished difference on exotic introgressions in Brassica napus

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The Crop Journal 2021年第5期9卷 1169-1178页

作者： Dandan Hu Yusheng Zhao jinxiong shen Xiangxiang He Yikai Zhang Yong Jiang Rod Snowdon Jinling Meng Jochen C.Reif Jun Zou National Key Laboratory of Crop Genetic Improvement College of Plant Science&TechnologyHuazhong Agricultural UniversityWuhan 430070HubeiChina Department of Breeding Research Leibniz Institute of Plant Genetics and Crop Plant Research(IPK)Stadt Seeland 06466Germany Department of Plant Breeding IFZ Research Centre for BiosystemsLand Use and NutritionJustus Liebig UniversityGiessen 35392Germany

Extensive exotic introgression could significantly enlarge the genetic distance of hybrid parental populations to promote strong *** goal of this study was to investigate whether genome-wide prediction can support pre-breeding in populations with exotic *** evaluated seed yield,seed yield related traits and seed quality traits of 363 hybrids of Brassica napus (AACC) derived from two parental populations divergent on massive exotic introgression of related species in three *** hybrids presented strong heterosis on seed yield,which was much higher than other investigated *** genomic best linear unbiased prediction models considering the exotic introgression and different marker effects (additive,dominance,and epistatic effects) were constructed to test the prediction ability for different traits of the *** analysis showed that the trait complexity,exotic introgression,genetic relationship between the training set and testing set,training set size,and environments affected the prediction *** models with best prediction ability for different traits ***,relatively high prediction ability (e.g.,0.728 for seed yield) was also observed when the simplest models were used,excluding the effects of the special exotic introgression and epistasis effect by5-fold cross validation,which would simplify the prediction for the trait with complex architecture for hybrids with exotic *** results provide novel insights and strategies for genome-wide prediction of hybrids between genetically distinct parent groups with exotic introgressions.

关键词： Hybrid Genome-wide prediction Exotic introgression Brassica napus Heterosis

*** affects chloroplast development in Brassica napus L.

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The Crop Journal 2021年第5期9卷 992-1002页

作者： Tingting Liu Baolong Tao Hanfei Wu Jing Wen Bin Yi Chaozhi Ma Jinxing Tu Tingdong Fu Lixia Zhu jinxiong shen National Key Laboratory of Crop Genetic Improvement National Center of Rapeseed ImprovementHuazhong Agricultural UniversityWuhan 430070HubeiChina School of Biological Science and Technology University of JinanJinan 250000ShandongChina

Mature chloroplasts,as the main sites of photosynthesis,are essential for seedling growth in higher *** of function of genes involved in chloroplast development changes plant *** obtained a YELLOW COTYLEDON (YCO) mutant in rapeseed (Brassica napus L.) using ***,a gene of unknown function,has two homologous copies (Bna *** and Bna ***) in *** mutation of these two homologs resulted in yellow cotyledons and chlorotic true *** electron microscopy revealed that the formation of thylakoid membranes was inhibited in yellow *** similarity search revealed that YCO was conserved in different species,and a subcellular location assay verified that *** was located in the *** was expressed in multiple tissues,most highly in *** of *** blocked the transcription of plastid genes,especially those of photosystem genes transcribed by plastid-encoded *** sequencing showed that the majority of genes involved in ribosome assembly and photosynthesis were down-regulated in *** *** results suggested that loss of function of *** affected plastid gene transcription,which influenced chloroplast biogenesis in rapeseed seedlings.

关键词： Brassica napus Chlorophyll biosynthesis Chloroplast biogenesis Chlorotic leaves Yellow cotyledon

Bna ***1,encoding a tetratricopeptide repeat protein,is required for early chloroplast biogenesis in Brassica napus

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The Crop Journal 2022年第3期10卷 597-610页

作者： Haiyan Zhang Xiaoting Li Yebitao Yang Kaining Hu Xianming Zhou Jing Wen Bin Yi jinxiong shen Chaozhi Ma Tingdong Fu Jinxing Tu National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina

Chloroplasts are essential for plant growth and development,as they play a key role in *** chloroplast biogenesis process is complex and its regulatory mechanism remains *** characterized a spontaneous Brassica napus(rapeseed)mutant,ytg,that showed a delayed greening phenotype in all green organs and retarded *** identified Bna ***1 encoding a chloroplastlocalized tetratricopeptide repeat protein widely expressed in rapeseed *** speculated that the ytg phenotype was caused by the deletion of Bna ***1 based on sequence comparison of 4608(with normal green leaves,isolated from the elite Chinese rapeseed cultivar ZS11)and ytg combined with transcriptome data and CRISPR/Cas9 gene editing *** homologous gene(Bna ***1)restored the phenotype of the *** ***1 interacted with MORF2,MORF8,and *** editing of the ndh D-2,ndh F-290,pet L-5,and ndh G-50 plastid transcripts was affected in *** findings suggested that Bna ***1 participates in RNA editing *** predicted 29 RNA editing sites in the chloroplast of Brassica napus by comparison with the Arabidopsis chloroplast *** conclude that Bna ***1 affects the posttranscriptional regulation of plastid gene expression and suggest that a tetratricopeptide repeat protein is involved in the chloroplast RNA editing in rapeseed.

关键词： Brassica napus Chloroplast MORFs RNA editing TPR

A review: status and prospect of heterosis prediction in rapeseed(Brassica napus L.)

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Oil Crop Science 2017年第3期2卷 187-194页

作者： Lifang Wang Fang Zhang Tingdong Fu jinxiong shen Key Labortory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture Oil Crops Research InstituteChinese Academy of Agricultural Sciences Wuhan China College of Plant Science and Technology Huazhong Agricultural University Wuhan China Graduate School Chinese Academy of Agricultural Sciences Beijing China The National Agro-tech Extension and Service Center Beijing China

Heterosis has been widely exploited and utilized in rapeseed breeding. Reliable and precise prediction techniques of heterosis contributed in accelerating the crossbreeding and reducing the cost of large-scale field evaluation. Combined with our work in last decades, this review summarized the progress on heterosis prediction of rapeseed (Brassica napus L.), compared the efficiency of different methods, and proposed its prospect.

关键词： Brassica napus L. heterosis prediction combining ability molecular markers

Tribenuron-Methyl Induces Male Sterility through Anther-Specific Inhibition of Acetolactate Synthase Leading to Autophagic Cell Death

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Molecular Plant 2015年第12期8卷 1710-1724页

作者： Lun Zhao Xue Jing Li Chen Yingjun Liu Yanan Su Tingting Liu Changbin Gao Bin Yi Jing Wen Chaozhi Ma Jinxing Tu Jitao Zou Tingdong Fu jinxiong shen National Key Laboratory of Crop Genetic Improvement National Center of Rapeseed Improvement Huazhong Agricultural University Wuhan 430070 China National Research Council Canada Saskatoon Saskatchewan S7N 0W9 Canada

Tribenuron-methyl （TM） is a powerful sulfonylurea herbicide that inhibits branched-chain amino acid （BCAA） biosynthesis by targeting the catalytic subunit （CSR1） of acetolactate synthase （ALS）. Selective in- duction of male sterility by foliar spraying of TM at low doses has been widely used for hybrid seed produc- tion in rapeseed （Brassica napus）; however, the underlying mechanism remains unknown. Here, we report greater TM accumulation and subsequent stronger ALS inhibition and BCAA starvation in anthers than in leaves and stems after TM application. Constitutive or anther-specific expression of csrl-lD （a CSR1 mutant） eliminated anther-selective ALS inhibition and reversed the TM-induced male sterile phenotype in both rapeseed and Arabidopsis. The results of TM daub-stem experiments, combined with the observations of little TM accumulation in anthers and reversion of TM-induced male sterility by targeted expression of the TM metabolism gene Bel in either the mesophyll or phloem, suggested that foliar-sprayed TM was polar-transported to anthers mainly through the mesophyll and phloem. Microscopy and immunoblotting revealed that autophagy, a bulk degradation process induced during cell death, was elevated in TM-induced male sterile anthers and by anther-specific knockdown of ALS. Moreover, TM-induced pollen abortion was significantly inhibited by the autophagy inhibitor 3-MA. These data suggested that TM was polar-transported to anthers, resulting in BCAA starvation via anther-specific ALS inhibition and, ulti- mately, autophagic cell death in anthers.

关键词： male sterility chemical hybridizing agent acetolactate synthase amino acid starvation autophagy,Brassica napus

Asymmetric epigenome maps of subgenomes reveal imbalanced transcription and distinct evolutionary trends in Brassica napus

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Molecular Plant 2021年第4期14卷 604-619页

作者： Qing Zhang Pengpeng Guan Lun Zhao Meng Ma Liang Xie Yue Li Ruiqin Zheng Weizhi Ouyang Shunyao Wang Hongmeijuan Li Ying Zhang Yong Peng Zhilin Cao Wei Zhang Qin Xiao Yuanling Xiao Tingdong Fu Guoliang Li Xingwang Li jinxiong shen National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China National Engineering Research Center of Rapeseed Huazhong Agricultural UniversityWuhan 430070China Agicultural Bioinformatics Key Laboratory of Hubei Province Hubei Engineering Technology Research Center of Agricutural Big Data3D Genomics Research CenterCollge of InformaticsHuazhong Agricultural UniversityWuhan 430070China These authors contributed equally to this article.

The complexity of the epigenome landscape and transcriptional regulation is significantly increased during plant polyploidization,which drives genome evolution and contributes to the increased adaptability to diverse ***,a comprehensive epigenome map of Brassica napus is still *** this study,we performed integrative analysis of five histone modifications,RNA polymerase Ⅱ CCU-pancy,DNA methylation,and transcriptomes in two *** lines(2063A and B409),and established global maps of regulatory elements,chromatin states,and their dynamics for the whole genome(including the An and Cn subgenomes)in four tissue types(young leaf,flower bud,silique,and root)of these two *** 65.8% of the genome was annotated with different epigenomic *** with the Cn subgenome,the An subgenome possesses a higher level of active epigenetic marks and lower level of repressive epigenetic *** from subgenome-unique regions contribute to the major differences between the An and Cn *** histone modifications between homeologous gene pairs reflect their biased expression *** identified a novel bivalent chromatin state(with H3K4me1 and H3K27me3)in *** that is associated with tissue-specific gene ***,we observed that different types of duplicated genes have discrepant patterns of histone modification and DNA methylation ***,our findings provide a valuable epigenetic resource for allopolyploid plants.

关键词： Brassica napus epigenome ChlP-seq gene expression

基于MODIS数据的Landsat-7 SLC-off影像修复方法研究

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测绘学报 2010年第3期39卷 251-256页

作者：朱长明沈占锋骆剑承杨辽胡晓东沈金祥中国科学院遥感应用研究所北京100101 中国科学院研究生院北京100049 中国科学院新疆生态与地理研究所新疆乌鲁木齐830011

研究如何修复由于扫描行校正器SLC故障而造成的数据坏行,成为国际遥感研究的热点。文章在总结现有的修复方法基础上提出用相同时相的MODIS09数据修复SLC-off数据的方法。该方法采用时空自适应反射率融合模型(STARFM)算法实现MODIS09数据... 详细信息

研究如何修复由于扫描行校正器SLC故障而造成的数据坏行,成为国际遥感研究的热点。文章在总结现有的修复方法基础上提出用相同时相的MODIS09数据修复SLC-off数据的方法。该方法采用时空自适应反射率融合模型(STARFM)算法实现MODIS09数据和TM/ETM+数据的融合,获得与SLC-off数据相同时相和空间分辨率的融合数据,然后采用局部线性直方图匹配完成对SLC-off数据填充。实验证明该方法修复效果明显好于传统方法,解决了传统修复方法中存在的一些问题,一定程度上提高了ETM+SLC-off数据的利用率。

关键词： SLC-off 缝隙数据修复 MODIS ETM+

Functional genomics of Brassica napus:Progresses,challenges,and perspectives

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Journal of Integrative Plant Biology 2024年第3期66卷 484-509页

作者： Zengdong Tan Xu Han Cheng Dai Shaoping Lu Hanzi He Xuan Yao Peng Chen Chao Yang Lun Zhao Qing-Yong Yang Jun Zou Jing Wen Dengfeng Hong Chao Liu Xianhong Ge Chuchuan Fan Bing Yi Chunyu Zhang Chaozhi Ma Kede Liu jinxiong shen Jinxing Tu Guangsheng Yang Tingdong Fu Liang Guo Hu Zhao National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China Yazhouwan National Laboratory Sanya 572025China

Brassica napus,commonly known as rapeseed or canola,is a major oil crop contributing over 13%to the stable supply of edible vegetable oil *** and understanding the gene functions in the *** genome is crucial for genomic breeding.A group of genes controlling agronomic traits have been successfully cloned through functional genomics studies in *** this review,we present an overview of the progress made in the functional genomics of ***,including the availability of germplasm resources,omics databases and cloned functional *** on the current progress,we also highlight the main challenges and perspectives in this *** advances in the functional genomics of *** contribute to a better understanding of the genetic basis underlying the complex agronomic traits in *** and will expedite the breeding of high quality,high resistance and high yield in *** varieties.

关键词： accelerate breeding Brassica napus functional genomics high resistance high yield