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Purification and immunoglobulin E epitopes identification of low molecular weight glutenin:an allergen in Chinese wheat

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Food Science and Human Wellness 2023年第3期12卷 720-727页

作者： Yanbo Wang Yihang Tong jinru zhou Dong Yang Linglin Fu Food Safety Key Laboratory of Zhejiang Province School of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China College of Food Science and Nutritional Engineering China Agricultural UniversityBeijing 100083China

As one of the most important cereals,wheat(Triticum aestivum)has high nutritional value and is widely cultivated in ***,wheat can cause severe allergic reactions,and a growing number of people are developing allergies to Chinese *** molecular weight glutenin(LMW-GS),an important allergen in susceptible populations,is responsible for celiac disease and wheat contacts *** this study,LMW-GS was highly purified from Chinese wheat(Xiaoyan 6)and further identified and *** addition,8 peptides were predicted efficiently by 5 immunological tools,among which 5 peptides showed significant immunoglobulin E binding *** specific epitopes were found to be in the non-conserved region of the amino acid sequence of LMW-GS,which was speculated to be the specific epitope of Chinese *** systematic research of LMW-GS may provide new insights into the prevention of wheat allergy and development of hypoallergenic wheat products.

关键词： Triticum aestivum Allergen Low molecular weight glutenin(LMW-GS) Immunological tools

A novel AgNPs/MOF substrate-based SERS sensor for high-sensitive on-site detection of wheat gluten

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Food Science and Human Wellness 2024年第2期13卷 681-687页

作者： Linglin Fu Yanzhuo Du jinru zhou Huan Li Minzi Wang Yanbo Wang Food Safety Key Laboratory of Zhejiang Province School of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China Zhejiang Engineering Research Institute of Food&Drug Quality and Safety School of Management and E-BusinessZhejiang Gongshang UniversityHangzhou 310018China Zhejiang Dien Biotech Co. Ltd.Hangzhou 310030China

Gluten,known as the major allergen in wheat,has gained increasing concerns in industrialized countries,resulting in an urgent need for accurate,high-sensitive,and on-site detection of wheat gluten in complex food ***,we proposed a silver nanoparticles(AgNPs)/metal-organic framework(MOF)substrate-based surface-enhanced Raman scattering(SERS)sensor for the high-sensitive on-site detection of wheat *** detection occurred on the newly in-situ synthesized AgNPs/MOF-modified SERS substrate,providing an enhancement factor(EF)of 1.89×10^(5).Benefitting from the signal amplification function of AgNPs/MOF and the superiority of SERS,this sensor represented high sensitivity performance and a wide detection range from 1×10^(-15)mol/L to 2×10^(-6)mol/L with a detection limit of 1.16×10^(-16)mol/L,which allowed monitoring the trace of wheat gluten in complex food system without matrix *** reliable sandwich SERS sensor may provide a promising platform for high-sensitive,accurate,and on-site detection of allergens in the field of food safety.

关键词： Wheat allergy Gluten Surface-enhanced Raman scattering Metal-organic framework DNA recognition

An antifouling polydopamine-based fluorescent aptasensor for determination of arginine kinase

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Food Science and Human Wellness 2023年第3期12卷 737-744页

作者： Yanbo Wang Huan Li jinru zhou Fangting Wang Yifan Qian Linglin Fu Food Safety Key Laboratory of Zhejiang Province School of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China

Simple yet efficient detection methods for food allergens are in urgent need to help people avoid the risks imposed by allergenic *** this work,a polydopamine(PDA)-based fluorescent aptasensor was developed to detect arginine kinase(AK),one of the major allergens in *** aptamer towards AK was firstly selected via systematic evolution of ligands by exponential enrichment method and labeled with fluorescein amidite(FAM)to build a fluorescence resonance energy transfer(FRET)system with PDA *** glycol(PEG)was employed to construct an antifouling surface for the aptasensor to eliminate food matrix *** the presence of AK,the PDA-based aptasensor exhibited elevated fluorescent signals as the FAM-labeled aptamer bound to AK and detached from the PDA *** aptasensor showed great stability and resistance to nonspecific interference of background proteins and had a limit of detection(LOD)of 0.298μg/*** proposed aptasensor was further proved to be feasible for quantitative analysis of AK in nine species of shrimps and five commercial processed products,which indicated its high potential in tracing the presence of AK in complex aquatic products.

关键词： Food allergy Aptasensor Antifouling Polydopamine FRET effect

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Molecular basis of the allergenicity and cross-reactivity of a major allergen-Crassostrea gigas arginine kinase

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Food Science and Human Wellness 2024年

作者： Yanbo Wang PeiAo Zhang jinru zhou Jihui Gao Jinlyu Sun Di Wu Wentong Xue Dong Yang Linglin Fu Food Safety Key Laboratory of Zhejiang Province， School of Food Science and Biotechnology， Zhejiang Gongshang University College of Food Science & Nutritional Engineering， China Agricultural University Allergy Department， State Key Laboratory of Complex Severe and Rare Diseases， Peking Union Medical College Hospital， Chinese Academy of Medical Sciences and Peking Union Medical College Institute for Global Food Security， School of Biological Sciences， Queen's University of Belfast， 19 Chlorine Gardens

As an important shellfish group， the oyster can induce severe allergic reactions. We aimed to identify and characterize the major allergen in Crassostrea gigas， and elucidate the molecular basis of its allergenicity and cross-reactivity. The native and recombinant C. gigas-AK displayed significant IgG- and IgE-binding activity. The IgE-binding activity of C. gigas-AK could be reduced by thermal treatment and strong acidic and alkaline conditions. Besides， cross-reactivity of AK was demonstrated between shellfish species. Among seven epitope peptides identified here， P2 is responsible for the specificity of C. gigas-AK， while P3 is responsible for cross-reactions between mollusks and crustaceans. Furthermore， Glu98 and His31 in the light chain， Arg101， and Lys57 in the heavy chain are identified as key IgE residues in recognizing epitopes. These findings provide new insights into the prevention and treatment of shellfish allergy and the development of hypoallergenic shellfish products.

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大学英语课程思政教育路径探索与实践

九江学院学报（社会科学版） 2024年第1期43卷 52-57页

作者：周津茹运城师范高等专科学校外语系山西运城044000

在新时期全面推进课程思政教育的背景下,大学英语作为一门公共基础课,具备自己的语言学科特点和实施课程思政的优势。文章通过对具体教学实践进行分析与展示,深入探索大学英语课程思政的实施路径,精准定位思政融入点,精心设计教学过程,... 详细信息

在新时期全面推进课程思政教育的背景下,大学英语作为一门公共基础课,具备自己的语言学科特点和实施课程思政的优势。文章通过对具体教学实践进行分析与展示,深入探索大学英语课程思政的实施路径,精准定位思政融入点,精心设计教学过程,以提升课程思政的育人实效。

关键词：大学英语课程思政实践探索

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基于职业能力培养的英语教学模式改革研究

淮南职业技术学院学报 2023年第6期23卷 120-122页

作者：周津茹运城师范高等专科学校山西运城044000

社会经济高速发展视域下,社会对高校人才的英语综合能力提出更高要求,加强学生职业能力培养至关重要。因此,基于职业能力培养背景下,剖析大学英语教学现状,提出围绕职教特点,重构教学目标、结合职业导向,更新教学内容、分层教学方法,组... 详细信息

社会经济高速发展视域下,社会对高校人才的英语综合能力提出更高要求,加强学生职业能力培养至关重要。因此,基于职业能力培养背景下,剖析大学英语教学现状,提出围绕职教特点,重构教学目标、结合职业导向,更新教学内容、分层教学方法,组织协作学习、依托定期培训,完善师资队伍、实施多元评价,夯实职业能力等改革对策,全方位提高大学生的英语职业能力,使学生成长为适应社会经济发展的高素质人才。

关键词：职业能力英语教学模式

文化自信视域下中华文化融入高职英语教学实践研究

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晋城职业技术学院学报 2023年第5期16卷 71-74,78页

作者：周津茹运城师范高等专科学校山西运城044000

高职英语课程应坚持以培养具有中国情怀、国际视野和跨文化技能的高素质人才为宗旨。以《创新高职英语1》第二单元Fashion(时装)为例,有效将中华文化元素融入日常教学各环节,以生动的中国故事增加文化自信,培养民族自豪感,开阔国际视野。

关键词：文化自信中华文化高职英语

不同硅酸镁锂浓度水凝胶支架的制备及促成骨性能的比较研究

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中华创伤骨科杂志 2022年第6期24卷 522-527页

作者：苗胜周津如刘斌郝晓甜裴国献毕龙空军军医大学西京医院骨科西安710032 郑州大学附属洛阳中心医院影像科洛阳471000 解放军北部战区总医院骨科沈阳110015 西北大学生命科学学院西安710069 南方科技大学医院深圳518055

目的制备不同硅酸镁锂浓度的水凝胶支架,并比较不同浓度硅酸镁锂的促成骨性能。方法在明胶/海藻酸钠水凝胶中按质量百分比为0%、1%、2%、3%加入硅酸镁锂,分别为T0、T1、T2、T3组,测定各组的压缩模量和24 h浸提液的离子含量。体外实验使... 详细信息

目的制备不同硅酸镁锂浓度的水凝胶支架,并比较不同浓度硅酸镁锂的促成骨性能。方法在明胶/海藻酸钠水凝胶中按质量百分比为0%、1%、2%、3%加入硅酸镁锂,分别为T0、T1、T2、T3组,测定各组的压缩模量和24 h浸提液的离子含量。体外实验使用各组浸提液培养基及普通培养基(空白组)(n=3)培养骨髓间充质干细胞(BMSCs),测定培养7 d后成骨基因Runt相关转录因子2(Runx2)、碱性磷酸酶(ALP)、Ⅰ型胶原的表达。体内实验将支架植入大鼠股骨髁缺损处,并设置不植入支架的空白组(n=3),应用苏木精-伊红(HE)染色和免疫组织化学染色观察各组的成骨修复情况。结果T2组的压缩模量为(139.05±6.43)kPa,显著高于T0、T1、T3组[(68.83±3.76)、(101.18±3.68)、(125.40±3.28)kPa],差异均有统计学意义(P0.05);但均大于T1组,差异有统计学意义(P<0.05)。体外实验结果显示:T2组Runx2、ALP、Ⅰ型胶原的表达分别为1.59±0.11、2.02±0.08、1.06±0.17,均高于其他各组,差异均有统计学意义(P<0.05)。HE染色发现植入的水凝胶与组织结合紧密。免疫组织化学染色显示T2组的Runx2及骨钙素阳性细胞数量明显多于其他组。结论硅酸镁锂水凝胶具有理想的生物相容性,能缓释硅酸镁锂分解离子,促进BMSCs成骨分化,在体内可促进骨缺损的修复,且硅酸镁锂浓度为2%时效果最佳。

关键词：硅酸镁锂水凝胶骨髓细胞成骨分化骨修复

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粘接固定桥的固位及研究进展

国际口腔医学杂志 2016年第5期43卷 537-541页

作者：陈西文周进茹朱智敏口腔疾病研究国家重点实验室华西口腔医院修复科(四川大学) 成都610041

粘接桥因其微创的优点重新受到推崇,而修复体材料、粘接面的处理技术和粘接剂系统等的发展也给予其新的应用发展机会。本文对粘接桥的固位研究和发展进行综述,以期更新口腔修复医生对粘接桥的认识,使这种微创而有效的修复方法受到应有... 详细信息

粘接桥因其微创的优点重新受到推崇,而修复体材料、粘接面的处理技术和粘接剂系统等的发展也给予其新的应用发展机会。本文对粘接桥的固位研究和发展进行综述,以期更新口腔修复医生对粘接桥的认识,使这种微创而有效的修复方法受到应有的重视。

关键词：粘接固定桥粘接技术固位力