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Content Determination of Kaempferol,Jaceosidin and Eupatilin in the Moxa Roll and Moxa Cone from Different Producing Areas by HPLC

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Medicinal Plant 2017年第5期8卷 14-19,26页

作者： Li CAO jinqing lu Xin YE Jiamin MENG Hubei Provincial Medicinal Plants Research and Development Center/Hubei University of Chinese Medicine

[Objectives] To determine the content of main active flavonoids in the moxa roll and moxa cone from different producing areas and provide a reference for the quality control of finished moxa roll and moxa cone. [Methods]Using HPLC,with kaempferol,jaceosidin and eupatilin as reference substance,there comparative analysis was done on the content of kaempferol,jaceosidin and eupatilin in the moxa roll and moxa cone from different producing areas. [Results]11 lots of samples contained kaempferol,jaceosidin and eupatilin,but the content differed significantly. [Conclusions] There was a large difference in the content of three kinds of flavonoids in the moxa roll and moxa cone from different producing areas,and the study provided a reference for the quality evaluation and quality control of moxa roll and moxa cone.

关键词： Moxa roll Moxa cone HPLC

Preparation of Botanical Pesticide 16% Chijunning Microemulsion

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Plant Diseases and Pests 2013年第2期4卷 25-28页

作者： lu jinqing Li Xiaoshuang Liang Huang Dai Yi He Xuefeng Hubei University of Traditional Chinese Medicine/Hubei Province Established R & D Center

By screening emulsifiers and co-solvents and determining relevant physical and chemical properties, the preparation method for 16% Chijunning micreemulsion was studied in the paper. The best formulation （volume fraction） was as follows： Rad/x phytolaccae extract 4%, Magnolia offtcinalis extract 4%, Xanthium sibiricum extract 8%, DBS-Ca 10%, pesticide emulsifier A 15%, cyclohexanone 20%, water 39%. The microemulsion had better development and market future because of its good stability, low price, use security and environmental protection.

关键词： Botanical pesticides Tobacco brown spot Micreemulsion Preparation

Analysis of Low Melting Point Fat Constituents of Black Soybeans by HS-SPME-GC-MS

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Medicinal Plant 2017年第5期8卷 4-5,10页

作者： Han TU Hanmei JIANG jinqing lu Hongyu ZHANG Xin YE Li CAO Research and Development Center of Medicinal Plant in Hubei Province/Hubei University of Chinese Medicine

[Objectives] To analyze the low melting point fat constituents in the black soybeans with green and yellow heart and their relative content,and compare the differences in the low melting point fat constituents between different kinds of black soybeans. [Methods] Using HS-SPME-GC-MS,the qualitative analysis was performed on the low melting point fat constituents of black soybeans; using peak area normalization method,the relative content of constituents was calculated. [Results]A total of 42 peaks were identified from the low melting point fat constituents of the black soybeans with yellow heart,and 18 kinds of chemical constituents were identified,accounting for 81.39% of total relative content of low melting point fat constituents; a total of 37 peaks were identified from the low melting point fat constituents of the black soybeans with green heart,and 15 kinds of chemical constituents were identified,accounting for 83.24% of total relative content of low melting point fat constituents. There were 9 kinds of common chemical constituents for the two kinds of black soybeans,and 5-allylguaiacol had the highest relative content,followed by hexanol. [Conclusions] There was no significant difference in the low melting point fat constituents between two kinds of black soybeans.

关键词： Black soybeans HS-SPME GC-MS Low melting point Fat

Laboratory and Field Evaluation of lupo Compound Miroemulsion(LCM) against Alternaria alternata in Tobacco

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Plant Diseases and Pests 2014年第2期5卷 18-21页

作者： lu jinqing Dai Yi Li Xiaoshuang Liang Huan Cai Junlong Li Qiang Guo Shengnan Medicinal Plant Research and Development Center of Hubei Province Hubei University of Chinese Medicine

[Objective]The paper was to study effect of lupo compound microemulsion( LCW) on Alternaria alternata. [Method]The activity of spore germination and mycelial growth was investigated by filter paper method and mycelial growth rate method,and field test was also conducted to determine the effect of LCW on tobacco plants. [Result]LCW could inhibit spore germination and mycelial growth of A. alternate. The higher concentration led to more severe reduction of sporulation and heavier inhibition against mycelial growth. When LCW was diluted into 200 times,inhibition rate of sporulation reached 91. 23%,and the inhibition rate of mycelial growth reached 93. 50%. Field test also proved that LCW had significant inhibitory effect on tobacco brown spot disease. [Conclusion]The study provided the reference for using botanical pesticide to control A. alternate in fields.

关键词： Alternaria alternata Botanical fungicide lupo compound miroemulsion Disease control

Determination of Volatile Constituents,Total Flavonoids and Total Polysaccharides in the Roots of Artemisia argyi Levl. et Van. *** cv. Qiai

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Medicinal Plant 2017年第6期8卷 1-4,9页

作者： Min GONG jinqing lu Yushuo XIAO Jiamin MENG Jingling HUANG Mengfei LIU Jiaxuan XIA Yi ZHOU Hubei University of Chinese Medicine/Research and Development Center of Medicinal Plant in Hubei Province

[Objectives] To analyze the volatile components,the contents of total flavonoids and total polysaccharides of the roots of Artemisia argyi Levl. et Van. var. argyi cv. Qiai,and to provide a scientific basis for the comprehensive development and utilization of the resources.[Methods]The volatile components of the roots were extracted by solid phase micro-extraction( SPME),and its volatile components were analyzed by gas chromatography-mass spectrometry( GC-MS); the contents of total flavonoids and total polysaccharides in the samples were measured by UV-spectrophotometry. [Results] 44 peaks were isolated from the roots and 30 components were identified,accounting for 80. 9% of the total volatile components; the content of total flavonoids in the roots was 9. 42%; the content of total polysaccharides in the roots was 11. 05%.[Conclusions] After a comprehensive investigation,the olefins in the roots generally have antibacterial activity,the contents of the total flavonoids and total polysaccharides contained in the roots were relatively high,the roots of Artemisia argyi do have broad prospects for the development.

关键词： The roots of Artemisia argyi Solid phase micro-extraction(SPME) Gas chromatography-mass spectrometry(GC-MS) Total flavonoids Total polysaccharides

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锥形撒肥圆盘中肥料颗粒运动模型优化与试验

农业机械学报 2018年第6期49卷 85-91,111页

作者：吕金庆孙贺兑瀚李紫辉李季成于佳钰东北农业大学工程学院哈尔滨150030

针对锥形撒肥圆盘存在抛施肥均匀性差、相关理论和解析模型研究较少等问题,建立了肥料颗粒在锥形撒肥圆盘上及空气中的运动模型。分析锥形撒肥圆盘结构和运动参数对肥料颗粒自旋性的影响,将肥料颗粒的自旋性充分考虑在整个运动过程中,... 详细信息

针对锥形撒肥圆盘存在抛施肥均匀性差、相关理论和解析模型研究较少等问题,建立了肥料颗粒在锥形撒肥圆盘上及空气中的运动模型。分析锥形撒肥圆盘结构和运动参数对肥料颗粒自旋性的影响,将肥料颗粒的自旋性充分考虑在整个运动过程中,进而得到影响抛撒均匀性及抛撒幅宽的主要因素。采用正交试验方案研究了叶片长度、叶片倾角、锥形撒肥圆盘转速对肥料颗粒抛撒的横向变异系数的影响。对正交试验结果进行方差和极差分析,结果表明:叶片长度为145 mm、叶片倾角为0°、锥形撒肥圆盘转速为1 200 r/min时,抛撒的横向变异系数为5.80%,满足抛施肥作业要求。该研究可提高马铃薯锥盘式撒肥机施肥作业效率,为锥盘式撒肥机的设计提供理论参考。

关键词：锥形撒肥圆盘肥料颗粒抛施肥运动模型

GC-MS Analysis of Volatile Components Extracted from Fruit of Artemisia argyi

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Medicinal Plant 2017年第3期8卷 10-13页

作者： Xin YE jinqing lu Li CAO Research and Development Center of Medicinal Plants in Hubei Province/Hubei University of Traditional Chinese Medicine

[Objectives] To analyze the volatile components extracted from fruit of Artemisia argyi.[Methods] The volatile components from fruit of Artemisia argyi were extracted by steam distillation( SD) and headspaces solid-phase microextraction( HS-SPME). The components were separated and analyzed by gas chromatography-mass spectrometry( GC-MS) with the area normalization method to determine the relative percentage content of each component.[Results]33 compounds were isolated and 29 compounds were identified by SD accounting for about93. 02% of the total volatile components; and 35 compounds were isolated and 30 compounds were identified by HS-SPME accounting for about99. 01% of the total volatile components. 11 kinds of the volatile components were identified by the two methods together and the content of β-thujone was highest.[Conclusions] There were some differences between SD and HS-SPME,and the two methods were applied to the extraction of different types of volatile components,and combination of the two methods to analyze the volatile components in fruit of Artemisia argyi could help us to get more comprehensive information.

关键词： SD HS-SPME GC-MS Fruit of Artemisia argyi Volatile components

顶空固相微萃取-气质联用技术分析川芎酒炙前后的挥发性成分

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中国药房 2017年第28期28卷 3945-3948页

作者：曹利卢金清叶欣王樊湖北中医药大学药学院/湖北省药用植物研发中心武汉430065

目的:比较川芎生品和酒炙品挥发性成分的差异,为川芎的药理活性研究及临床应用提供参考。方法:采用顶空固相微萃取-气质联用技术分析川芎酒炙前后的挥发性成分,并运用面积归一化法计算各成分的相对百分含量。结果:从川芎生品中分离出28... 详细信息

目的:比较川芎生品和酒炙品挥发性成分的差异,为川芎的药理活性研究及临床应用提供参考。方法:采用顶空固相微萃取-气质联用技术分析川芎酒炙前后的挥发性成分,并运用面积归一化法计算各成分的相对百分含量。结果:从川芎生品中分离出28个成分,鉴定出24个成分,占总挥发性成分的99.70%,含量较高的分别为2-甲基-2,3-二氢-1H-茚-2-醇(20.06%)、α-芹子烯(17.97%)及4-乙基-壬烯-5-炔(9.24%)。从川芎酒炙品中分离出21个成分,鉴定出18个成分,占总挥发性成分的89.74%,含量最高的为4-乙基-壬烯-5-炔(12.97%),其次为4-蒈烯(11.74%)和α-芹子烯(10.79%);与生品相比,有效镇痛成分α-蒎烯、β-榄香烯含量有所增加。结论:川芎酒炙前后挥发性成分及相对百分含量存在一定差异,酒炙后挥发性成分相对减少,但有镇痛作用的成分含量增加,镇痛效果优于生品。

关键词：川芎生品酒炙品挥发性成分顶空固相微萃取气质联用

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辊式马铃薯分级机设计与试验

农业机械学报 2019年第2期50卷 323-332页

作者：吕金庆于佳钰冯雪李紫辉李季成刘中原东北农业大学工程学院哈尔滨150030

针对我国现有马铃薯分级机普遍存在的分级等级少、分级效率差等问题,设计了一种新型辊式马铃薯分级机。对该机器的工作原理进行了阐述。通过理论计算对其关键部件进行设计,确定了分选装置的结构参数;对马铃薯运动过程进行分析,确定出影... 详细信息

针对我国现有马铃薯分级机普遍存在的分级等级少、分级效率差等问题,设计了一种新型辊式马铃薯分级机。对该机器的工作原理进行了阐述。通过理论计算对其关键部件进行设计,确定了分选装置的结构参数;对马铃薯运动过程进行分析,确定出影响马铃薯分级机分级效率和分级精度的因素,利用ADAMS仿真软件对马铃薯分级机分级性能进行仿真试验,得出马铃薯运动位移曲线,与结构理论相吻合。以马铃薯的上料量、辊子转速、提升装置的提升角为试验因素,以马铃薯分级机的分级精度和分级效率为试验指标进行样机试验,试验结果表明:马铃薯的上料量为55 t/h、辊子转速为120 r/min、提升角为24°时,其分级精度为96%,分级效率为53. 7 t/h,满足马铃薯分级机的作业要求。

关键词：马铃薯分级机提升装置