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Simulation and fabrication of in vitro microfluidic microelectrode array chip for patterned culture and electrophysiological detection of neurons

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Nanotechnology and Precision Engineering 2024年第2期7卷 1-10页

作者： Yan Yang Shihong Xu Yu deng Yaoyao Liu Kui Zhang Shiya Lv Longze Sha Qi Xu Xinxia Cai jinping Luo State Key Laboratory of Transducer Technology Aerospace Information Research InstituteChinese Academy of SciencesBeijing 100190China School of Electronic Electrical and Communication EngineeringUniversity of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China

To enable the detection and modulation of modularized neural networks in vitro,this study proposes a microfluidic microelectrode array chip for the cultivation,compartmentalization,and control of neural cells.The chip was designed based on the specific structure of neurons and the requirements for detection and modulation.Finite-element analysis of the chip’s flow field was conducted using the COMSOL Multiphysics software,and the simulation results show that the liquid within the chip can flow smoothly,ensuring stable flow fields that facilitate the uniform growth of neurons within the microfluidic channels.By employing MEMS technology in combination with nanomaterial modification techniques,the microfluidic microelectrode array chip was fabricated successfully.Primary hippocampal neurons were cultured on the chip,forming a well-defined neural network.Spontaneous electrical activity of the detected neurons was recorded,exhibiting a 23.7%increase in amplitude compared to neuronal discharges detected on an open-field microelectrode array.This study provides a platform for the precise detection and modulation of patterned neuronal growth in vitro,potentially serving as a novel tool in neuroscience research.

关键词： Microﬂuidic Microelectrode array In vitro Neural chip

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A review of the amino acid metabolism in placental function response to fetal loss and low birth weight in pigs

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Journal of Animal Science and Biotechnology 2022年第4期13卷 987-998页

作者： Chengquan Tan Zihao Huang Wenyu Xiong Hongxuan Ye jinping deng Yulong Yin Guangdong Provincial Key Laboratory of Animal Nutrition Control National Engineering Research Center for Breeding Swine IndustryInstitute of Subtropical Animal Nutrition and FeedCollege of Animal ScienceSouth China Agricultural UniversityGuangzhou 510642GuangdongChina National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production Institute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125HunanChina

The fertility of sows mainly depends on the embryo losses during gestation and the survival rate of the postfarrowing piglets.The selection of highly-prolific sows has been mainly focused on the selection of genotypes with high ovulatory quota.However,in the early-and post-implantation stages,the rate of embryo losses was increased with the increase of zygotes.Among the various factors,placental growth and development is the vital determinant for fetal survival,growth,and development.Despite the potential survival of fetuses with deficient placental development,their life-conditions and growth can be damaged by a process termed intrauterine growth retardation(IUGR).The newborn piglets affected by IUGR are prone to increased morbidity and mortality rates;meanwhile,the growth,health and welfare of the surviving piglets will remain hampered by these conditions,with a tendency to exacerbate with age.Functional amino acids such as glycine,proline,and arginine continue to increase with the development of placenta,which are not only essential to placental growth(including vascular growth)and development,but can also be used as substrates for the production of glutathione,polyamines and nitric oxide to benefit placental function in many ways.However,the exact regulation mechanism of these amino acids in placental function has not yet been clarified.In this review,we provide evidence from literature and our own work for the role and mechanism of dietary functional amino acids during pregnancy in regulating the placental functional response to fetal loss and birth weight of piglets.This review will provide novel insights into the response of nutritionally nonessential amino acids(glycine and proline)to placental development as well as feasible strategies to enhance the fertility of sows.

关键词： Amino acids Birth weight Fetal loss Pigs Placenta

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Dietary fibers with low hydration properties exacerbate diarrhea and impair intestinal health and nutrient digestibility in weaned piglets

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Journal of Animal Science and Biotechnology 2023年第2期14卷 771-791页

作者： Shuangbo Huang Zhijuan Cui Xiangyu Hao Chuanhui Cheng Jianzhao Chen Deyuan Wu Hefeng Luo jinping deng Chengquan Tan Guangdong Provincial Key Laboratory of Animal Nutrition Control National Engineering Research Center for Breeding Swine IndustryInstitute of Subtropical Animal Nutrition and FeedCollege of Animal ScienceSouth China Agricultural UniversityGuangzhou 510642GuangdongChina Dekon Food and Agriculture Group Chengdu 610031China Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural UniversityGuangzhou 510642GuangdongChina.

Background:This study aimed to investigate the hydration properties of different-source fibrous materials by com-paring their water-binding capacity(WBC),water swelling capacity(WSC),viscosity,and in vivo effects of selected samples on growth performance,nutrient digestibility,diarrhea,and intestinal health in weaned piglets.Methods:A total of 13 commercially available fibrous materials were first compared in chemical composition and in vitro hydration property.Subsequently,40 weaned piglets were randomized to five experimental dietary groups(8 piglets per group):control diet(a basal diet without dietary fiber,CON),basal diet supplemented with 5%microcrys-talline cellulose(MCC),5%wheat bran(WB),5%Moringa oleifera leaf powder(MOLP),or 5%sugar beet pulp(SBP),fol-lowed by analyzing their growth performance and diarrhea rate in a 28-d experiment.After the feeding experiment,anaesthetized piglets were killed,and their intestinal and colon content or plasma samples were analyzed in nutrient digestibility,intestinal morphology,intestinal barrier,short-chain fatty acids(SCFAs),and bacterial population.Results:In vitro studies showed low hydration properties for WB and MCC,while medium hydration properties for MOLP and SBP.In vivo studies indicated that compared with medium hydration property groups,low hydration prop-erty groups showed(1)exacerbated diarrhea,impaired intestinal health,and reduced apparent fecal digestibility of dry matter,gross energy,acid detergent fiber,and neutral detergent fiber;(2)decreased SCFAs concentration and rela-tive levels of Lactobacillus and Bifidobacterium,but increased levels of Escherichia coli and Brachyspira hyodysenteriae in colon contents.Additionally,SBP showed optimal performance in reducing diarrhea and increasing SCFAs produc-tion.Correlation analysis revealed a positive correlation of fiber hydration properties with in vitro SCFAs production,and diarrhea index and nutrient digestibility were negatively and positively correlated with SCFAs levels in the colon contents of weaned

关键词： Diarrhea Dietary fiber Hydration property Intestinal health Nutrient digestibility Weaned piglet

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Maternal supply of cysteamine alleviates oxidative stress and enhances angiogenesis in porcine placenta

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Journal of Animal Science and Biotechnology 2022年第1期13卷 181-196页

作者： Shuangbo Huang Zifang Wu Zihao Huang Xiangyu Hao Longmiao Zhang Chengjun Hu Jianfu Wei jinping deng Chengquan Tan Guangdong Provincial Key Laboratory of Animal Nutrition Control National Engineering Research Center for Breeding Swine IndustryInstitute of Subtropical Animal Nutrition and FeedCollege of Animal ScienceSouth China Agricultural UniversityGuangzhou 510642GuangdongChina Guangzhou DaBeiNong Agri-animal Huabandry Science and Technology Co. Ltd.Guangzhou 510642GuangdongChina Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural UniversityGuangzhou 510642GuangdongChina

Background:Oxidative stress in placenta is associated with the occurrence of adverse pregnancy outcomes in sow,but there are few satisfactory treatment strategies for these conditions.This study investigated the potential of cysteamine(CS)as an antioxidant protectant for regulating the reproductive performance,redox status,and placental angiogenesis of sows.Methods:The placental oxidative stress status and vascular density of piglets with different birth weights:<1.0 kg(low birth weight,LBW)and 1.4–1.6 kg(normal birth weight,NBW)were evaluated,followed by allotting 84 sows to four treatments(n=21)and feeding them with a basal diet supplemented with 0,100,300,or 500 mg/kg of CS from d 85 of gestation to d 21 of lactation,respectively.Placenta,serum,and colostrum samples of sows or piglets were collected,and the characteristics of sows and piglets were recorded.Furthermore,the in vivo results were validated using porcine vascular endothelial cells(PVECs).Results:Compared with the NBW placentae,the LBW placentae showed increased oxidative damage and were vulnerable to angiogenesis impairment.Particularly,H2O2-induced oxidative stress prompted intracellular reactive oxygen species generation and inhibited the tube formation and migration of PVECs as well as the expression of vascular endothelial growth factor-A(VEGF-A)in vitro.However,dietary CS supplementation can alleviate oxidative stress and improve the reproductive performance of sows.Specifically,compared with the control group,dietary 100 mg/kg CS could(1)decrease the stillbirth and invalid rates,and increase both the piglet birth weight in the low yield sows and the placental efficiency;(2)increase glutathione and reduce malondialdehyde in both the serum and the colostrum of sows;(3)increase the levels of total antioxidant capacity and glutathione in LBW placentae;(4)increase the vascular density,the mRNA level of VEGF-A,and the immune-staining intensity of platelet endothelial cell adhesion molecule-1 in the LBW placentae.Furthermore,the in vitro experiment

关键词： Angiogenesis Cysteamine Oxidative stress Placenta Sow

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BAK1 plays contrasting roles in regulating abscisic acid-induced stomatal closure and abscisic acid-inhibited primary root growth in Arabidopsis

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Journal of Integrative Plant Biology 2022年第6期64卷 1264-1280页

作者： jinping deng Lingyao Kong Yinhua Zhu Dan Pei Xuexue Chen Yu Wang Junsheng Qi Chunpeng Song Shuhua Yang Zhizhong Gong State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China College of Life Sciences Qingdao UniversityQingdao 266071China Collaborative Innovation Center of Crop Stress Biology Institute of Plant Stress BiologyHenan UniversityKaifeng 475001HenanChina Institute of Life Science and Green Development School of Life SciencesHebei UniversityBaoding 071002China

The mechanisms that balance plant growth and stress responses are poorly understood, but they appear to involve abscisic acid(ABA) signaling mediated by protein kinases. Here, to explore these mechanisms, we examined the responses of Arabidopsis thaliana protein kinase mutants to ABA treatment. We found that mutants of BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED RECEPTOR KINASE 1(BAK1) were hypersensitive to the effects of ABA on both seed germination and primary root growth. The kinase OPEN STOMATA 1(OST1) was more highly activated by ABA in bak1 mutant than the wild type. BAK1 was not activated by ABA treatment in the dominant negative mutant abi1-1 or the pyr1 pyl4 pyl5 pyl8 quadruple mutant, but it was more highly activated by this treatment in the abi1-2 abi2-2 hab1-1 loss-of-function triple mutant than the wild type. BAK1 phosphorylates OST1 T146 and inhibits its activity. Genetic analyses suggested that BAK1 acts at or upstream of core components in the ABA signaling pathway, including PYLs, PP2 Cs,and Sn RK2 s, during seed germination and primary root growth. Although the upstream brassinosteroid(BR) signaling components BAK1 and BR INSENSITIVE 1(BRI1) positively regulate ABAinduced stomatal closure, mutations affecting downstream components of BR signaling, including BRASSINOSTEROID-SIGNALING KINASEs(BSKs)and BRASSINOSTEROID-INSENSITIVE 2(BIN2), did not affect ABA-mediated stomatal movement. Thus,our study uncovered an important role of BAK1 in negatively regulating ABA signaling during seed germination and primary root growth, but positively modulating ABA-induced stomatal closure, thus optimizing the plant growth under drought stress.

关键词： abscisic acid BAK1 primary root growth stomatai closure

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Ca^(2+)-dependent successive phosphorylation of vacuolar transporter MTP8 by CBL2/3-CIPK3/9/26 and CPK5 is critical for manganese homeostasis in Arabidopsis

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Molecular Plant 2022年第3期15卷 419-437页

作者： Chuanfeng Ju Zhenqian Zhang jinping deng Cuicui Miao Zhangqing Wang Lukas Wallrad Laiba Javed Dali Fu Ting Zhang Jörg Kudla Zhizhong Gong Cun Wang State Key Laboratory of Crop Stress Biology for Arid Areas College of Life Sciencesand Institute of Future AgricultureNorthwest Agriculture&Forestry UniversityYanglingShaanxi 712100China State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Institut fur Biologie und Biotechnologie der Pflanzen Westfälische Wilhelms-Universität48149 MünsterGermany College of Life Sciences Hebei UniversityBaodingChina

Manganese(Mn)is an essential micronutrient for all living organisms.However,excess Mn supply that can occur in acid or waterlogged soils has toxic effects on plant physiology and development.Although a variety of Mn transporter families have been characterized,we have only a rudimentary understanding of how these transporters are regulated to uphold and adjust Mn homeostasis in plants.Here,we demonstrate that two calcineurin-B-like proteins,CBL2/3,and their interacting kinases,CIPK3/9/26,are key regulators of plant Mn homeostasis.Arabidopsis mutants lacking CBL2 and 3 or their interacting protein kinases CIPK3/9/26 exhibit remarkably high Mn tolerance.Intriguingly,CIPK3/9/26 interact with and phosphorylate the tonoplast-localized Mn and iron(Fe)transporter MTP8 primarily at Ser35,which is conserved among MTP8 proteins from various species.Mn transport complementation assays in yeast combined with multiple physiological assays indicate that CBL-CIPK-mediated phosphorylation of MTP8 negatively regulates its transport activity from the cytoplasm to the vacuole.Moreover,we show that sequential phosphorylation of MTP8,initially at Ser31/32 by the calcium-dependent protein kinase CPK5 and subsequently at Ser35 by CIPK26,provides an activation/deactivation fine-tuning mechanism for differential regulation of Mn transport.Collectively,our findings define a two-tiered calcium-controlled mechanism for dynamic regulation of Mn homeostasis under conditions of fluctuating Mn supply.

关键词： manganese Ca^(2+) calcineurin B-like proteins(CBLs) CBL-interacting protein kinases(CIPKs) Mn transporter MTP8 calcium-dependent protein kinases(CPKs)

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本安型信息矿灯在煤矿“三网合一”系统中的应用

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现代制造技术与装备 2024年第1期60卷 80-82页

作者：邓金萍大同裕隆环保有限责任公司大同037024

本安型信息矿灯作为一种集成了信息化和安全性的照明设备,在煤矿井下人员定位系统、矿灯智能充电系统及虹膜考勤系统中有着重要作用。文章对本安型信息矿灯的特点和其在煤矿“三网合一”系统中的应用展开研究,并探讨其未来的应用趋势。

关键词：本安型信息矿灯三网合一信息化安全性

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Imbalanced dietary methionine-to-sulfur amino acid ratio can affect amino acid profiles, antioxidant capacity, and intestinal morphology of piglets

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Animal Nutrition 2020年第4期6卷 447-456页

作者： Miaomiao Bai Lei Wang Hongnan Liu Kang Xu jinping deng Ruilin Huang Yulong Yin College of Animal Science South China Agricultural UniversityGuangzhou 510642China Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry ProductionKey Laboratory of Agro-ecological Processes in Subtropical RegionHunan Provincial Engineering Research Center for Healthy Livestock and Poultry ProductionScientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-CentralMinistry of AgricultureInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China Laboratory of Animal Nutrition and Human Health Hunan International Joint Laboratory of Animal Intestinal Ecology and HealthCollege of Life ScienceHunan Normal UniversityChangsha 410081China Hunan Co-Innovation Center of Safety Animal Production(CICSAP) Changsha 410128China

Animal protein sources such as fishmeal and plasma powder are excellent and indispensable sources of energy,amino acid s,and minerals in animal production.Amino acid imbalance,especially methionine-to-sulfur amino acid(Met:SAA)ratio,caused by an imbalance of animal protein meal leads to growth restriction.This study was conducted to evaluate the effects of imbalanced Met:SAA ratio supplementation of different animal protein source diets on growth performance,plasma amino acid profiles,antioxidant capacity and intestinal morphology in a piglet model.Twenty-four weaned piglets(castrated males;BW=10.46±0.34 kg),assigned randomly into 3 groups(8 piglets/group),were fed for 28 d.Three experimental diets of equal energy and crude protein levels were as follows:1)a corn-soybean basal diet with a Met:SAA ratio at 0.51(BD);2)a plasma powder diet with a low Met:SAA ratio at 0.41(L-MR);3)a fishmeal diet with a high Met:SAA ratio at0.61(H-MR).Results revealed that compared to BD,L-MR significantly decreased(P<0.05)the activities of plasma total antioxidant capacity and glutathione peroxidase,plasma amino acid profiles,and significantly reduced(P<0.05)villus height and crypt depth in the duodenum and jejunum.Additionally,L-MR significantly reduced(P<0.05)the mRNA expression level of solute carrier family 7 member 9(SIC7 A9)in the ileum,and significantly increased(P<0.05)mRNA expression levels of zonula occludens-1(ZO-1)in the duodenum,and Claudin-1,ZO-1,sodium-coupled neutral amino acid transporters 2(SNAT2)and SIC7 A7 in the j ejunum.HMR significantly increased(P<0.05)plasma SAA levels,and significantly reduced(P<0.05)average daily feed intake,villus height,and villus height-to-crypt depth(VH:CD)ratio in the ileum compared to BD.In conclusion,L-MR may result in oxidative stress and villous atrophy but proves beneficial in improving intestinal barrier function and the activity of amino acid transporters for compensatory growth.H-MR may impair intestinal growth and development for weaned piglets.The research provides a guidanc

关键词： Animal protein source Methionine-to-sulfur amino acid ratio Antioxidant capacity Intestinal morphology Piglet

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Abscisic acid signaling negatively regulates nitrate uptake via phosphorylation of NRT1.1 by SnRK2s in Arabidopsis

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Journal of Integrative Plant Biology 2021年第3期63卷 597-610页

作者： Hang Su Tian Wang Chuanfeng Ju jinping deng Tianqi Zhang Mengjiao Li Hui Tian Cun Wang State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences Northwest A&F UniversityYangling 712100China State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China College of Natural Resources and Environment Northwest A&F UniversityYangling 712100China

Nitrogen(N)is a limiting nutrient for plant growth and productivity.The phytohormone abscisic acid(ABA)has been suggested to play a vital role in nitrate uptake in fluctuating N environments.However,the molecular mechanisms underlying the involvement of ABA in N deficiency responses are largely unknown.In this study,we demonstrated that ABA signaling components,particularly the three subclass Ⅲ SUCROSE NON-FERMENTING1(SNF1)-RELATED PROTEIN KINASE 2 S(SnRK2)proteins,function in root foraging and uptake of nitrate under N deficiency in Arabidopsis thaliana.The snrk2.2 snrk2.3 snrk2.6 triple mutant grew a longer primary root and had a higher rate of nitrate influx and accumulation compared with wild-type plants under nitrate deficiency.Strikingly,SnRK2.2/2.3/2.6 proteins interacted with and phosphorylated the nitrate transceptor NITRATE TRANSPORTER1.1(NRT1.1)in vitro and in vivo.The phosphorylation of NRT1.1 by SnRK2 s resulted in a significant decrease of nitrate uptake and impairment of root growth.Moreover,we identified NRT1.1Ser585 as a previously unknown functional site:the phosphomimetic NRT1.1S585 D was impaired in both low-and high-affinity transport activities.Taken together,our findings provide new insight into how plants fine-tune growth via ABA signaling under N deficiency.

关键词： abscisic acid nitrogen deficiency NRT1.1 Sn RK2.2/2.3/2.6

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Dietary supplementation with betaine or glycine improves the carcass trait,meat quality and lipid metabolism of finishing mini-pigs

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Animal Nutrition 2021年第2期7卷 376-383页

作者： Yinzhao Zhong Zhaoming Yan Bo Song Changbing Zheng Yehui Duan Xiangfeng Kong jinping deng Fengna Li Guangdong Provincial Key Laboratory of Animal Nutrition Regulation South China Agricultural UniversityGuangzhou 510642China Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process Changsha 410125China Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production Changsha 410125China National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production Changsha 410125China Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central Ministry of AgricultureChangsha 410125China University of Chinese Academy of Sciences Beijing 100039China College of Animal Science and Technology Hunan Agricultural UniversityChangsha 410128China

The objective of the study is to evaluate and compare the effects of betaine or glycine on carcass trait,meat quality and lipid metabolism of finishing Huan Jiang mini-pigs.Betaine called trimethylglycine is a methyl derivative of glycine,but few researches were conducted to compare the impact of dietary betaine and glycine on pigs.One hundred and forty-four Huan Jiang mini-pigs(body weight=10.55±0.15 kg;70 d)were randomly divided to 3 treatment groups(basal diet,glycine or betaine).Results indicated that dietary betaine increased the average daily gain(ADG)and final weight(P<0.05).Dietary glycine or betaine markedly reduced average backfat thickness(P<0.05)and heightened lean percentage(P<0.01)compared to the control group.Moreover,in comparison with the control group,betaine significantly improved the redness(a*)and tenderness(shear force)of the longissimus dorsi(LD)muscle(P<0.05),whereas glycine only raised the value of a*of the LD muscle(P<0.05).These results showed that diet supplemented with 0.25%betaine and equimolar amounts of glycine could regulate cascass trait and meat quality of finishing Huan Jiang mini-pigs,and the effect of betaine was superior to that of glycine.

关键词： Betaine Glycine Meat quality Lipid metabolism Huan jiang mini-pig

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