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Scaling up of cluster beam deposition technology for catalysis application

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Frontiers of Chemical Science and Engineering 2021年第6期15卷 1360-1379页

作者： Giuseppe Sanzone jinlong yin Hailin Sun Teer Coatings Ltd. DroitwichWorcestershire WR99ASUK Quantum Solid-State Physics Department of Physics and AstronomyB-3001 LeuvenBelgium

Many research works have demonstrated that the combination of atomically precise cluster deposition and theoretical calculations is able to address fundamental aspects of size-effects,cluster-support interactions,and reaction mechanisms of cluster *** the wet chemistry method has been widely used to synthesize nanoparticles,the gas-phase synthesis and size-selected strategy was the only method to prepare supported metal clusters with precise numbers of atoms for a long ***,the low throughput of the physical synthesis method has severely constrained its wider adoption for catalysis *** this review,we introduce the latest progress on three types of cluster source which have the most promising potential for scale-up,including sputtering gas aggregation source,pulsed microplasma cluster source,and matrix assembly cluster *** the sputtering gas aggregation source is leading ahead with a production rate of~20 mg·h^(-1),the pulsed microplasma source has the smallest physical dimensions which makes it possible to compact multiple such devices into a small volume for multiplied production *** matrix assembly source has the shortest development history,but already show an impressive deposition rate of~10 mg·h^(-1).At the end of the review,the possible routes for further throughput scale-up are envisaged.

关键词： nanoparticle cluster cluster beam deposition magnetron sputtering heterogeneous catalysis

乡村庭院景观创新设计模式探索与实践——以晋江英林镇西埔村为例

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工业设计 2024年第3期 60-63页

作者：付琳尹金龙丁铮福建农林大学风景园林与艺术学院福州350000

研究以乡村庭院景观与经济产业的融合为切入点,探讨乡村庭院的研究现状、存在问题与发展的现实意义,提出庭院景观与经济产业融合的五种“新”的庭院设计模式,以福建省泉州市晋江市英林镇西埔村为设计实践基地,结合实地调研的情况,对存... 详细信息

研究以乡村庭院景观与经济产业的融合为切入点,探讨乡村庭院的研究现状、存在问题与发展的现实意义,提出庭院景观与经济产业融合的五种“新”的庭院设计模式,以福建省泉州市晋江市英林镇西埔村为设计实践基地,结合实地调研的情况,对存在问题进行具体分析,针对问题提出策略,并将提出的创新型设计模式应用于规划中。以期通过深入研究乡村庭院的营造模式,为优化和提升乡村庭院的设计与发展提供理论依据和实践指导。

关键词：乡村庭院景观经济产业西埔村

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双电加热三室RTO的阀门控制方案对比

工业炉 2024年第1期46卷 61-64页

作者：殷金龙吴谦王超尚龙飞王坤中国汽车工业工程有限公司蔚来汽车科技有限公司

三室RTO需要在短时间内改变气体流动方向，来达到循环加热和冷却蓄热体的目的，而切换阀的控制直接影响着系统的热效率和处理能力。根据阀门切换时间的不同以及反吹阀跟随开启的时间，试验得出RTO系统在不同的阀门切换时间作用下的响应... 详细信息

三室RTO需要在短时间内改变气体流动方向，来达到循环加热和冷却蓄热体的目的，而切换阀的控制直接影响着系统的热效率和处理能力。根据阀门切换时间的不同以及反吹阀跟随开启的时间，试验得出RTO系统在不同的阀门切换时间作用下的响应状态。通过对比试验数据，得出了双电加热三室RTO的最佳阀门控制逻辑。

关键词：三室RTO 阀门切换热效率控制逻辑

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采用双电加热的三室RTO温度控制方案对比

工业炉 2024年第2期46卷 56-59页

作者：吴谦殷金龙王超尚龙飞邢鹏超刘天力中国汽车工业工程有限公司

对于配有两组电加热的三室RTO，加热炉膛有多种不同的控制方案。和单组热源的炉膛不同，两组热源的炉膛存在针对不同传感器的不同温度控制方案。根据电加热器的布置和不同温度传感器的位置，通过试验得出了4种不同控制方案下炉膛温度的... 详细信息

对于配有两组电加热的三室RTO，加热炉膛有多种不同的控制方案。和单组热源的炉膛不同，两组热源的炉膛存在针对不同传感器的不同温度控制方案。根据电加热器的布置和不同温度传感器的位置，通过试验得出了4种不同控制方案下炉膛温度的变化曲线，对比试验数据后得到了不同要求下最优的温度控制方案。

关键词：三室RTO 双电加热温度控制

Endogenous viral elements in algal genomes

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Acta Oceanologica Sinica 2014年第2期33卷 102-107页

作者： WANG Liang WU Shuangxiu LIU Tao SUN Jing CHI Shan LIU Cui LI Xingang yin jinlong WANG Xumin YU Jun CAS Key Laboratory of Genome Sciences and Information Beijing Key Laboratory of Genome and Precision Medicine TechnologiesBeijing Institute of GenomicsChinese Academy of Sciences Beijing Key Laboratory of Functional Genomics for Dao-di Herbs Beijing Institute of GenomicsChinese Academy of Sciences University of Chinese Academy of Sciences College of Marine Life Science Ocean University of China

Endogenous viral elements （EVEs） are host-genomic fragments originated from viral genomes. They have been found universally in animal and plant genomes. Here we carried out a systematic screening and analy-sis of EVEs in algal genomes and found that EVEs commonly exist in algal genomes. We classified the EVE fragments into three categories according to the length of EVE fragments. Due to the probability of sequence similarity by chance, we ignored the potential function of medium-length EVE fragments. However, long-length EVE fragments probably had capability to encode protein domains or even entire proteins, and some short-length EVE fragments had high similarity with host＇s siRNA sequences and possibly served functions of small RNAs. Therefore, short and long EVE fragments might provide regulomic and proteomic novelty to the host＇s metabolism and adaptation. We also found several EVE fragments shared by more than 3 algal genomes. By phylogenetic analysis of the shared EVEs and their corresponding species, we found that the integration of viral fragments into host genomes was an ancient event, possibly before the divergence of Chlorophytes and Ochrophytes. Our findings show that there is a frequent genetic flow from viruses to algal genomes. Moreover, study on algal EVEs shed light on the virus-host interaction in large timescale and could also help us understand the balance of marine ecosystems.

关键词： endogenous viral elements algae genome transcriptome

Transcriptome sequencing of essential marine brown and red algal species in China and its significance in algal biology and phylogeny

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Acta Oceanologica Sinica 2014年第2期33卷 1-12页

作者： WU Shuangxiu SUN Jing CHI Shan WANG Liang WANG Xumin LIU Cui LI Xingang yin jinlong LIU Tao YU Jun CAS Key Laboratory of Genome Sciences and Information Beijing Key Laboratory of Genome and Precision Medicine TechnologiesBeijing Institute of GenomicsChinese Academy of Sciences Beijing Key Laboratory of Functional Genomics for Dao-di Herbs Beijing Institute of GenomicsChinese Academy of Sciences University of Chinese Academy of Sciences College of Marine Life Science Ocean University of China

Most phaeophytes （brown algae） and rhodophytes （red algae） dwell exclusively in marine habitats and play important roles in marine ecology and biodiversity. Many of these brown and red algae are also important resources for industries such as food, medicine and materials due to their unique metabolisms and me-tabolites. However, many fundamental questions surrounding their origins, early diversification, taxonomy, and special metabolisms remain unsolved because of poor molecular bases in brown and red algal study. As part of the 1 000 Plant Project, the marine macroalgal transcriptomes of 19 Phaeophyceae species and 21 Rhodophyta species from China＇s coast were sequenced, covering a total of 2 phyla, 3 classes, 11 orders, and 19 families. An average of 2 Gb per sample and a total 87.3 Gb of RNA-seq raw data were generated. Approxi-mately 15 000 to 25 000 unigenes for each brown algal sample and 5 000 to 10 000 unigenes for each red algal sample were annotated and analyzed. The annotation results showed obvious differences in gene expres-sion and genome characteristics between red algae and brown algae;these differences could even be seen between multicellular and unicellular red algae. The results elucidate some fundamental questions about the phylogenetic taxonomy within phaeophytes and rhodophytes, and also reveal many novel metabolic pathways. These pathways include algal CO2 fixation and particular carbohydrate metabolisms, and related gene/gene family characteristics and evolution in brown and red algae. These findings build on known algal genetic information and significantly improve our understanding of algal biology, biodiversity, evolution, and potential utilization of these marine algae.

关键词： Phaeophyceae brown algae Rhodophyta red algae marine macroalgae transcriptome sequencing secondary generation sequencing

The soybean GmPUB21-interacting protein GmDi19-5 responds to drought and salinity stresses via an ABA-dependent pathway

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The Crop Journal 2023年第4期11卷 1152-1162页

作者： Yunhua Yang Rui Ren Adhimoolam Karthikeyan jinlong yin Tongtong Jin Fei Fang Han Cai Mengzhuo Liu Dagang Wang Haijian Zhi Kai Li National Center for Soybean Improvement National Key Laboratory for Crop Genetics and Germplasm EnhancementKey Laboratory of Biology and Genetic Improvement of Soybean Ministry of AgricultureNanjing Agricultural UniversityNanjing 210095JiangsuChina College of Agronomy&Center for Crop Genome Engineering Henan Agricultural UniversityZhengzhou 450046HenanChina Subtropical Horticulture Research Institute Jeju National UniversityJeju 63243Republic of Korea

Drought-induced protein 19(Di19) is a Cys2/His2 zinc-finger protein that functions in plant growth and development and in tolerance to abiotic *** PUB21,an E3 ubiquitin ligase,negatively regulates drought and salinity response in *** identified potential interaction target proteins of Gm PUB21by yeast two-hybrid c DNA library screening,Gm Di19-5 as a *** fluorescence complementation and glutathionine-S-transferase pull-down assays confirmed the interaction between Gm Di19-5 and Gm *** Di19-5 was induced by Na Cl,drought,and abscisic acid(ABA) *** Di19-5 was expressed in the cytoplasm and *** Di19-5 overexpression conferred hypersensitivity to drought and high salinity,whereas Gm Di19-5 silencing increased drought and salinity *** of ABA-and stress response-associated genes including Gm RAB18 and Gm DREB2A were downregulated in Gm Di19-5-overexpressing plants under drought and salinity *** decreased the protein level of Gm Di19-5 in vivo,whereas Gm PUB21 increased the decrease of Gm Di19-5 after exogenous ABA *** accumulation of Gm PUB21 was also inhibited by Gm *** conclude that Gm PUB21 and Gm Di19-5 collaborate to regulate drought and salinity tolerance via an ABA-dependent pathway.

关键词： Soybean Drought and salinity stresses GmDi19-5 GmPUB21 ABA-dependent pathway

De novo sequencing and comparative analysis of three red algal species of Family Solieriaceae to discover putative genes associated with carrageenan biosysthesis

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Acta Oceanologica Sinica 2014年第2期33卷 45-53页

作者： SONG Lipu WU Shuangxiu SUN Jing WANG Liang LIU Tao CHI Shan LIU Cui LI Xingang yin jinlong WANG Xumin YU Jun CAS Key Laboratory of Genome Sciences and Information Beijing Key Laboratory of Genome and Precision Medicine TechnologiesBeijing Institute of GenomicsChinese Academy of Sciences Beijing Key Laboratory of Functional Genomics for Dao-di Herbs Beijing Institute of GenomicsChinese Academy of Sciences University of Chinese Academy of Sciences College of Marine Life Science Ocean University of China

Betaphycus gelatinus, Kappaphycus alvarezii and Eucheuma denticulatum of Family Solieriaceae, Order Gi-gartinales, Class Rhodophyceae are three important carrageenan-producing red algal species, which pro-duce different types of carrageenans, beta （β）-carrageenan, kappa （κ）-carrageenan and iota （ι）-carrageenan. So far the carrageenan biosynthesis pathway is not fully understood and few information is about the So-lieriaceae genome and transcriptome sequence. Here, we performed the de novo transcriptome sequencing, assembly, functional annotation and comparative analysis of these three commercial-valuable species using an Illumina short-sequencing platform Hiseq 2000 and bioinformatic software. Furthermore, we compared the different expression of some unigenes involved in some pathways relevant to carrageenan biosynthe-sis. We finally found 861 different expressed KEGG orthologs which contained a glycolysis/gluconeogenesis pathway （21 orthologs）, carbon fixation in photosynthetic organisms （16 orthologs）, galactose metabolism （5 orthologs）, and fructose and mannose metabolism （9 orthologs） which are parts of the carbohydrate me-tabolism. We also found 8 different expressed KEGG orthologs for sulfur metabolism which might be impor-tantly related to biosynthesis of different types of carrageenans. The results presented in this study provided valuable resources for functional genomics annotation and investigation of mechanisms underlying the biosynthesis of carrageenan in Family Solieriaceae.

关键词： Betaphycus gelatinus Kappaphycus alvarezii Eucheuma denticulatum Solieriaceae de novo transcriptome sequencing carrageenan

Comparative analysis of four essential Gracilariaceae species in China based on whole transcriptomic sequencing

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Acta Oceanologica Sinica 2014年第2期33卷 54-62页

作者： XU Jiayue SUN Jing yin jinlong WANG Liang WANG Xumin LIU Tao CHI Shan LIU Cui REN Lufeng WU Shuangxiu YU Jun CAS Key Laboratory of Genome Sciences and Information Beijing Key Laboratory of Genome and Precision Medicine TechnologiesBeijing Institute of GenomicsChinese Academy of Sciences Beijing Key Laboratory of Functional Genomics for Dao-di Herbs Beijing Institute of GenomicsChinese Academy of Sciences University of Chinese Academy of Sciences Changchun University of Chinese Medicine College of Marine Life Science Ocean University of China

Three Gracilaria species, G. chouae, G. blodgettii, G. vermiculophylla and a close relative species, Gracilari-opsis lemaneiformis which is now nominated as Gracilaria lemaneiformis, are the typically indigenous spe-cies which are important resources for the production of special proteins, phycobilisomes, special carbo-hydrates, and agar in China. In this study, de novo transcriptome sequencing on these four species using the next generation sequencing technology was performed for the first time. Functional annotations on assembled sequencing reads showed that the transcriptomic profiles were quite different between G. lema-neiformis and other three Gracilaria species. Comparative analysis of differential gene expression related to carbohydrate and phycobiliprotein metabolisms also showed that the expression profiles of these essential genes were different in four species. The genes encoding allophycocyanin, phycocyanin and phycoerythrin were further examined in four species and their deduced amino acid sequences were used for phylogenetic analysis to confirm that G. lemaneiformis had close relationship to genus Gracilaria, as well as that within genus Gracilaria, G. chouae had closer relationship to G. vermiculophylla rather than to G. blodgettii. The de novo transcriptome study on four species provided a valuable genomic resource for further understanding and analysis on biological and evolutionary study among marine algae.

关键词： Gracilaria chouae Gracilaria blodgettii Gracilaria vermiculophylla Gracilariopsis lemaneiformis transcriptome sequencing phycobiliprotein phylogeny