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Melatonin alleviates heat stress‑induced testicular damage in dairy goats by inhibiting the PI3K/AKT signaling pathway

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Stress Biology 2022年第1期2卷 120-135页

作者： Yundie Liu Hui Cai Xinrui Guo Aili Aierken jinlian hua Baohua Ma Sha Peng College of Veterinary Medicine Shaanxi Centre of Stem Cells Engineering&TechnologyNorthwest Agriculture and Forestry University of Science and TechnologyYangling 712100ShaanxiChina

Current measures mainly focus on how melatonin reduces physiological heat stress in animals,but its effects on reproductive damage to male dairy goats have been *** study aimed to determine the protective effect of melatonin on male reproduction during heat stress in dairy goats and to further explore its mechanisms.A natural heat stress model of Saanen dairy goats was used to assess testicular tissue damage 7days after heat stress and to examine semen quality changes during a spermatogenic ***-seq,Western blot,RT–qPCR,and immunofluo-rescence staining were used to explore the mechanism by which melatonin protects against heat stress-induced reproductive damage and to validate the *** data suggested that melatonin significantly alleviated the heat stress-induced decrease in sperm quality,protected varicose tubule structure,reduced the levels of heat shock proteins and apoptotic proteins and protected the spermatocytes and round spermatozoa,which are mainly affected by heat ***-seq results suggest that melatonin inhibits the PI3K/AKT signaling pathway,reduces the level of p-AKT,and promotes elevated *** addition,melatonin treatment could upregulate the gene expression of MT2 which was downregulated by heat stress and improve the change in extracellular matrix components and restore serum testosterone *** results suggest that melatonin can protect against testicular and spermatogenic cell damage and improve semen quality in male dairy goats under heat *** study provides an important reference for subsequent studies on the molecular mechanisms of melatonin in protecting male reproductive processes under heat stress and using exogenous melatonin to prevent heat stress.

关键词： Natural heat stress Melatonin PI3K/AKT signaling pathway Spermatocytes Apoptosis

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Reprogramming of the pig primordial germ cells into pluripotent stem cells: a brief review

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Frontiers of Agricultural Science and Engineering 2019年第1期6卷 28-32页

作者： Qijing LEI Qin PAN Shuai YU Na LI Shulin CHEN Kuldip SIDHU jinlian hua College of Veterinary Medicine/Shaanxi Centre of Stem Cells Engineering and Technology Northwest A&F University Centre for Healthy Brain Ageing UNSW Medicine

Primordial germ cells(PGCs) are regarded as unipotent cells that can produce only either spermatogonia or oocytes. However, PGCs can be converted into the pluripotent state by ?rst dedifferentiation to embryonic germ cells and then by reprogramming to induce them to become pluripotent stem cells(iPSCs). These two stages can be completely implemented with mouse cells. However, authentic porcine iPSCs have not been ***, we discuss recent advances in the stem cell ?eld for obtaining iPSCs from PGCs. This knowledge will provide some clues which will contribute to the regulation of reprogramming to pluripotency in farm species.

关键词： pig pluripotent stem cells primordial germ cells reprogramming

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Long-term assessment of risk factors for canine tumors registered in Xi’an, China

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Animal Diseases 2021年第4期1卷 268-275页

作者： Wei Wang Weihui Li Dianfeng Chu jinlian hua Xinke Zhang Dezhang Lu Yan Wang Shiqiang Zhang College of Veterinary Medicine Shaanxi Center of Stem Cells Engineering&TechnologyNorthwest A&F UniversityYangling712100China Xi’an Animal Hospital Northwest A&F UniversityYangling712100China Yebio Bioengineering Co. Ltd of QingdaoQingdao266000China State Key Laboratory of Genetically Engineered Veterinary Vaccines Shandong266114China

Tumors are one of the leading causes to death in pet dogs among diseases. The tumor incidence of pet dogs has been increasing, raising widespread concern. In this study, retrospective analysis was performed with 246 tumor cases registered in Xi’an Animal Hospital, Northwest A&F University from 2009 to 2018. Correlations of sex, age and breed with tumor incidences were evaluated. The results showed that reproductive system tumors occupied the highest proportion (39.84%), followed by cutaneous tumors (28.05%), digestive tumors (18.70%) and ocular tumor (4.47%). Among the reproductive system tumors, breast tumors are the most common tumor in female pet dogs, especially for Pekingese (11.43%). Female dogs with high susceptibility to breast tumors were at the ages of 6–18 years old. As far as cutaneous tumors were concerned, the male pet dogs at all ages, particularly Golden Retrievers (17.39%), showed a high incidence. By contrast, male Samoyed aged from 4 to 13 years had the highest incidence (15.22%) of digestive tumors. In addition, pet dogs with ocular tumors mainly happened at the ages of 0–1 years and 6–13 years. Collectively, our findings are significant to develop effective measures of medical surveillance for pet dogs’ health and will provide insights for comparative oncology.

关键词： Pet dogs Tumors Epidemiological analysis Risk factors

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Porcine pluripotent stem cells: progress, challenges and prospects

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Frontiers of Agricultural Science and Engineering 2019年第1期6卷 8-27页

作者： Jianyong HAN Yi-Liang MIAO jinlian hua Yan LI Xue ZHANG Jilong ZHOU Na LI Ying ZHANG Jinying ZHANG Zhonghua LIU State Key Laboratory for Agrobiotechnology College of Biological Sciences China Agricultural University Institute of Stem Cell and Regenerative Biology/College of Animal Science and Veterinary Medicine/Key Laboratory of Agricultural Animal Genetics Breeding and Reproduction of Ministry of Education Huazhong Agricultural University College of Veterinary Medicine/Shaanxi Centre of Stem Cells Engineering and Technology Northwest A&F University Key Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province College of Life Science Northeast Agricultural University

Pluripotent stem cells(PSCs) are characterized by their capacity for high self-renewal and multiple differentiation potential and include embryonic stem cells, embryonic germ cells and induced PSCs. PSCs provide a very suitable model for the studies of human diseases, drugs screening, regenerative medicine and developmental biology research. Pigs are considered as an ideal model for preclinical development of human xenotransplantation, therapeutic approaches and regenerative medicine because of their size and physiological similarity to humans. However, lack of knowledge about the derivation, characterization and pluripotency mechanisms of porcine PSCs hinders progress in these biotechnologies. In this review, we discuss the latest progress on porcine PSCs generation, evaluation criteria for pluripotency, the scienti?c and technical questions arising from these studies. We also introduce our perspectives on porcine PSC research, in the hope of providing new ideas for generating naive porcine PSCs and animal breeding.

关键词： embryonic germ cells embryonic stem cells induced pluripotent stem cells pigs pluripotent stem cells

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A multi-omics analysis of viral nucleic acid poly(I:C)responses to mammalian testicular stimulation

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Stress Biology 2024年第1期4卷 680-691页

作者： Donghui Yang Wenping Wu Qizhong Lu Yaling Mou Wenbo Chen Shicheng Wan Mengfei Zhang Congliang Wang Xiaomin Du Na Li jinlian hua Present Address:College of Veterinary Medicine Shaanxi Centre of Stem Cells Engineering&TechnologyNorthwest A&F UniversityNo.3rdTaicheng RoadYangling 712100ShaanxiChina State Key Laboratory of Biotherapy and Cancer Center West China HospitalSichuan UniversityChengdu 610041China Shaanxi Provincial Engineering and Technology Research Center of Cashmere Goats College of Life SciencesYulin UniversityYulin 719000ShaanxiChina

The male reproductive system has a standard immune response regulatory mechanism,However,a variety of external stimuli,including viruses,bacteria,heat,and medications can damage the testicles and cause orchitis and *** has been shown that various RNA viruses are more likely to infect the testis than DNA viruses,inducing orchitis and impairing testicular *** was found that local injection of the viral RNA analog poly(I:C)into the testes markedly disrupted the structure of the seminiferous tubules,accompanied by apoptosis and ***(I:C)mainly inhibited the expression of testosterone synthesis-associated proteins,STAR and MGARP,and affected the synthesis and metabolism of amino acids and lipids in the *** led to the disruption of the metabolite levels in the testis of mice,thus affecting the normal spermatogenesis *** present study analyzed the acute inflammatory response of the testis to viral infection using a multi-omics *** provides insights into how RNA virus infection impairs testicular function and offers a theoretical basis for future studies on immune homeostasis and responses under stress conditions in male reproduction.

关键词： Orchitis Poly(I:C) Proteomic Metabolomic Homeostasis

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Deficiency of microRNA miR-29a affects mouse spermatogenesis

Deficiency of microRNA miR-29a affects mouse spermatogenesis

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中国生理学会生殖科学专业委员会—中国动物学会生殖生物学分会第二次联合学术年会暨“生殖科学专业委员会第二次学术交流会”和“生殖生物学分会第十六次学术年会”

作者： Qin Pan Qijing Lei Wuzi Dong jinlian hua College of Veterinary Medicine Shaanxi Centre of Stem Cells Engineering and TechnologyNorthwest A&F University

A complicated modulation of gene expression occurs in ***,microRNAs emerged as important regulators of gene expression in many biological *** far,there have been reported that many miRNAs such as miR-34,miR-17-92,miR-16 play important roles in mouse ***,the germline roles and possible mechanisms of microRNA-29a(miR-29a)

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Plzf Effects on the Self-renewal and Proliferation in Goat Male Germline Stem Cells

Plzf Effects on the Self-renewal and Proliferation in Goat M...

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作者： Xiaomin Du Yuanxin Zhai Yudong Wei Fanglin Ma jinlian hua College of Veterinary Medicine Shaanxi Centre of Stem Cells Engineering & TechnologyNorthwest A&F University

Dairy goat is one of the most important livestock in China,however,the mechanism of the self-renewal and proliferation in dairy goat male germline stem cells(mGSCs)remained *** has been well demonstrated that Plzf is involved in self-renewal,pluripotency,differentiation,proliferation of mGSCs in mice,whereas little has been known in dairy *** this study,we

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Differentiation of neuron-like cells from mouse parthenogenetic embryonic stem cells

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Neural Regeneration Research 2013年第4期8卷 293-300页

作者： Xingrong Yan Yanhong Yang Wei Liu Wenxin Geng Huichong Du Jihong Cui Xin Xie jinlian hua Shumin Yu Liwen Li Fulin Chen College of Life Sciences Northwest University Department of Obstetrics & Gynecology Tangdu Hospitalthe Fourth Military Medical University of Chinese PLA College of Veterinary Medicine Northwest A&F University College of Veterinary Medicine Sichuan Agricultural University

Parthenogenetic embryonic stem cells have pluripotent differentiation potentials, akin to fertilized embryo-derived embryonic stem cells. The aim of this study was to compare the neuronal differentiation potential of parthenogenetic and fertilized embryo-derived embryonic stem cells. Before differentiation, karyotype analysis was performed, with normal karyotypes detected in both parthenogenetic and fertilized embryo-derived embryonic stem cells. Sex chromosomes were identified as XX. Immunocytochemistry and quantitative real-time PCR detected high expression of the pluripotent gene, Oct4, at both the mRNA and protein levels, indicating pluripotent differentiation potential of the two embryonic stem cell subtypes. Embryonic stern cells were induced with retinoic acid to form embryoid bodies, and then dispersed into single cells. Single cells were differentiated in N2 differentiation medium for 9 days. Immunocytochemistry showed parthenogenetic and fertilized embryo-derived embryonic stem cells both express the neuronal cell markers nestin, ~lll-tubulin and myelin basic protein. Quantitative real-time PCR found expression of neuregenesis related genes （Sox-1, Nestin, GABA, Pax6, Zic5 and Pitxl） in both types of embryonic stem cells, and Oct4 expression was significantly decreased. Nestin and Pax6 expression in parthenogenetic embryonic stem cells was significantly higher than that in fertilized embryo-derived embryonic stem cells. Thus, our experimental findings indicate that parthenogenetic embryonic stem cells have stronger neuronal differentiation potential than fertilized embryo-derived embryonic stem cells.

关键词： neural regeneration stem cells parthenogenesis parthenogenetic embryonic stem cells embryonic stem cells neuronal cells karyotypes Oct4 differentiation embryoid body mice grants-supported paper photographs-containing paper neuroregeneration

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人类胚胎生殖细胞体外分化为心肌细胞的研究

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生物医学工程学杂志 2006年第5期23卷 1080-1085页

作者：华进联徐小明窦忠英西北农林科技大学陕西省干细胞工程技术研究中心杨凌712100

胚胎生殖（EG）细胞是来源于胚胎原始生殖细胞（PGCs）的多潜能干细胞。采用无饲养层细胞、无细胞因子等的基础培养液（DMEM＋20％NBS＋0．1mM 2Me）培养EG细胞，部分在基础培养液添加10μmol／LRA＋0．75％DMSO或10μmol／L 5-氮胞苷（... 详细信息

胚胎生殖（EG）细胞是来源于胚胎原始生殖细胞（PGCs）的多潜能干细胞。采用无饲养层细胞、无细胞因子等的基础培养液（DMEM＋20％NBS＋0．1mM 2Me）培养EG细胞，部分在基础培养液添加10μmol／LRA＋0．75％DMSO或10μmol／L 5-氮胞苷（5-AZA）诱导人类EG细胞向心肌细胞分化，以检测其是否具有向心肌细胞自发分化的能力。9例（8．49％，9／106）胎儿EG细胞在体外分化得到20个节律性心脏跳动样细胞团，其搏动节律为20～120次／min，体外维持节律性搏动最短2d，最长至15d，呈PAS，Myoglobin，α-actin阳性；对K^＋、Ca^＋、肾上腺素等具有与在体心脏相似的反应性；透射电镜观察具有心肌细胞样结构。添加DMSO和RA或5-AZA诱导未得到跳动样心肌细胞，但可提高心肌α-actin免疫组织化学染色阳性率；表明人类胚胎生殖细胞具有向心肌细胞分化的潜能。

关键词：原始生殖细胞胚胎生殖细胞胚胎干细胞心肌细胞

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Prdm1在哺乳动物胚胎和生殖细胞发育中的作用

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中国细胞生物学学报 2014年第6期36卷 826-831页

作者：李娜吴江华进联西北农林科技大学动物医学院陕西省干细胞工程技术研究中心杨凌712100

Prdm1(PR domain zinc fi nger protein 1),又称为Blimp1(B-lymphocyte-induced maturation protein-1),是一个具有锌指结构的转录因子,通过调控多个基因的表达影响哺乳动物多种类型细胞的发育分化。从1991年发现至今,有关Prdm1的研究进展迅速,Prdm1在促进B细胞向浆细胞终末分化过程中的作用已经得到共识。但是,在小鼠及其他哺乳动物的胚胎发育过程中,尤其是关于Prdm1在生殖细胞发育分化中的作用机理研究则起步相对较晚。近期发现,在哺乳动物胚胎发育过程中,Prdm1在原始生殖细胞的形成、干细胞全能性的维持以及其他组织器官的形成中都发挥了重要的作用。

关键词： Prdm1 胚胎发育生殖细胞哺乳动物

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