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A k-mer-based pangenome approach for cataloging seed-storage-protein genes in wheat to facilitate genotype-to-phenotype prediction and improvement of end-use quality

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Molecular Plant 2024年第7期17卷 1038-1053页

作者： Zhaoheng Zhang Dan Liu Binyong Li Wenxi Wang Jize Zhang Mingming Xin Zhaorong Hu Jie Liu jinkun du Huiru Peng Chenyang Hao Xueyong Zhang Zhongfu Ni Qixin Sun Weilong Guo Yingyin Yao Frontiers Science Center for Molecular Design Breeding Key Laboratory of Crop Heterosis and Utilization(MOE)and Bejing Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijing100193China Key Laboratory of Crop Gene Resources and Breeding Institute of Crop ScienceChinese Academy of Agricultural SciencesBejingChina

Wheat is a staple foodfor more than 35%of the world's population,with wheatflourused to make hundreds of baked *** end-use quality is a major breeding target;however,improving it is especially time-consuming and ***,genes encoding seed-storage proteins(ssPs)form multigene families and are repetitive,with gaps commonplace in several genome *** overcome these barriers and efficiently identify superior wheat SSP alleles,we developed"PanSK"(Pan-SSP k-mer)for genotype-to-phenotype prediction based on an SsP-based pangenome *** uses 29-mer sequences that represent each ssP gene at the pangenomic level to reveal untapped diversity across landraces and modern ***-wide association studies with k-mers identified 23 Ssp genes associated with end-use quality that represent novel targets for *** evaluated the effect of rye secalin genes on end-use quality and found that removal of w-secalins from 1BL/1RS wheat translocation lines is associated with enhanced end-use ***,using machine-learning-based prediction inspired by PanSK,we predicted the quality phenotypes with high accuracy from genotypes *** study provides an effective approach for genome design based on ssP genes,enabling the breeding of wheat varieties with superior processing capabilities and improved end-use quality.

关键词： wheat seed-storage protein end-use quality k-mer pangenome genomic prediction

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Ectopic Overexpression of Wheat Adenosine Diphosphate-ribosylation Factor,TaARF,Increases Growth Rate in Arabidopsis

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Journal of Integrative Plant Biology 2009年第1期51卷 35-44页

作者： Yingyin Yao Zhongfu Ni jinkun du Zongfu Han Yan Chen Qingbo Zhang Qixin Sun Department of Plant Genetics & Breeding and State Key Laboratory for Agrobiotechnology Key Laboratory of Crop Heterosis and UtilizationMinistry of Education Key Laboratory of Crop Genomics and Genetic Improvement Ministry of Agdculture Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University Beijing 100094 China

Differential gene expression between hybrids and their parents is considered to be associated with heterosis. However, the physiological functions and possible contribution to heterosis of these differentially expressed genes are unknown. We have isolated one hybrid upregulated gene encoding putative wheat ADP-ribosylation factor, designated TaARF. In this study, real-time quantitative reverse transcription-polymerase chain reaction analysis indicated that the TaARF transcript was preferentially expressed in root, node and crown, and the accumulation of TaARF mRNA in hybrid was more than 1.5-fold higher than that in two parents. In order to understand possible roles of the putative wheat ARF gene, TaARF was overexpressed in Arabidopsis, and the transgenic plants were characterized. We show that ectopic overexpression of TaARF in Arabidopsis leads to increased leaf area, increased growth rate and earlier transition to flowering, suggesting that TaARF plays significant roles in growth and development. This study provides evidence demonstrating that TaARF plays important roles in growth and development and we speculate that the upregulated expression of this gene might contribute to the heterosis observed in wheat root and leaf growth.

关键词： adenosine diphosphate-ribosylation factor Arabidopsis ectopic heterosis overexpression wheat.

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A highly conserved amino acid in high molecular weight glutenin subunit 1Dy12 contributes to gluten functionality and processing quality in wheat

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Journal of Genetics and Genomics 2023年第11期50卷 909-912页

作者： Changfeng Yang Qian Chen Mingming Xin Zhenqi Su jinkun du Weilong Guo Zhaorong Hu Jie Liu Huiru Peng Zhongfu Ni Qixin Sun Yingyin Yao Frontiers Science Center for Molecular Design Breeding Key Laboratory of Crop Heterosis and Utilization(MOE)Beijing Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100193China

Bread wheat(Triticum aestivum L.)is one of the most commonly consumed staples worldwide,with widespread uses in foods such as breads,noodles,cakes,and cookies(Veraverbeke and Delcour,2002).The specific cooking properties of wheat products are mainly conferred by the gluten proteins contained in wheat grains(Delcour et al.,2012).High molecular weight glutenin subunits(HMW-GSs)are important constituents of wheat gluten proteins,largely determining gluten elasticity and processing quality(Branlard and Dardevet,1985;Payne et al.,1988).

关键词： weight wheat gluten

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Perception of fundamental science to boost lithium metal anodes toward practical application

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Green Energy & Environment 2024年第3期9卷 454-472页

作者： jinkun Wang Li Wang Hong Xu Li Sheng Xiangming He Institute of Nuclear and New Energy Technology Tsinghua UniversityBeijing100084China

As a key material for lithium metal batteries(LMBs),lithium metal is one of the most promising anode materials to break the bottleneck of battery energy density and a commonly used active material for reference *** lithium anodes are regarded as the holy grail of lithium batteries,decades of exploration have not led to the successful commercialization of LMBs,due mainly to the challenges related to the inherent properties of lithium *** pave the way for further investigation,herein,a comprehensive review focusing on the fundamental science of lithium are ***,the natures of lithium atoms and their isotopes,lithium clusters and lithium crystals are revisited,especially their structural and energetic ***,the electrochemical properties of lithium metal are *** important concepts and scientific questions,including the electronic structure of lithium,influence of high pressure and low temperature on the properties of lithium,factors influencing lithium deposition,generation of lithium dendrites,and electrode potential of lithium in different electrolytes,are explained and analyzed in *** to improve the performance of lithium anodes and thoughtfulness about the electrode potential in lithium battery research are proposed.

关键词： Lithium Cluster Crystal Physicochemical property Fundamental science

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Multiphase Interfacial Regulation Based on Hierarchical Porous Molybdenum Selenide to Build Anticorrosive and Multiband Tailorable Absorbers

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Nano-Micro Letters 2024年第1期16卷 85-105页

作者： Tianbao Zhao Zirui Jia jinkun Liu Yan Zhang Guanglei Wu Pengfei Yin College of Science Sichuan Agricultural UniversityYa’an 625014People’s Republic of China Institute of Materials for Energy and Environment State Key Laboratory of Bio‑Fibers and Eco‑TextilesCollege of Materials Science and EngineeringQingdao UniversityQingdao 266071People’s Republic of China College of Chemistry and Chemical Engineering Qingdao UniversityQingdao 266071People’s Republic of China

Electromagnetic wave(EMW)absorbing materials have an irreplaceable position in the field of military stealth as well as in the field of electromagnetic pollution *** in order to cope with the complex electromagnetic environment,the design of multifunctional and multiband high efficiency EMW absorbers remains a tremendous *** this work,we designed a three-dimensional porous structure via the salt melt synthesis strategy to optimize the impedance matching of the ***,through interfacial engineering,a molybdenum carbide transition layer was introduced between the molybdenum selenide nanoparticles and the three-dimensional porous carbon matrix to improve the absorption behavior of the *** analysis indicates that the number and components of the heterogeneous interfaces have a significant impact on the EMW absorption performance of the absorber due to mechanisms such as interfacial polarization and conduction loss introduced by interfacial ***,the prepared MoSe_(2)/MoC/PNC composites showed excellent EMW absorption performance in C,X,and Ku bands,especially exhibiting a reflection loss of−59.09 dB and an effective absorption bandwidth of 6.96 GHz at 1.9 *** coordination between structure and components endows the absorber with strong absorption,broad bandwidth,thin thickness,and multi-frequency absorption ***,it can effectively reinforce the marine anticorrosion property of the epoxy resin coating on Q235 steel *** study contributes to a deeper understanding of the relationship between interfacial engineering and the performance of EMW absorbers,and provides a reference for the design of multifunctional,multiband EMW absorption materials.

关键词： Interfacial engineering Anticorrosion Multiband Electromagnetic wave absorber

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Cloning and characterization of an up-regulated GA 20-oxidase gene in hybrid maize

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Progress in Natural Science:Materials International 2009年第2期19卷 161-166页

作者： jinkun du,Yingyin Yao,Zhongfu Ni,Qixin Sun Department of Plant Genetics & Breeding and State Key Laboratory for Agrobiotechnology,Key Laboratory of Crop Genomics and Genetic Improvement,Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement,China Agricultural University,Beijing 100094,China Department of Plant Genetics & Breeding and State Key Laboratory for Agrobiotechnology Key Laboratory of Crop Genomics and Genetic Improvement Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University Beijing 100094 China

Previous studies revealed that GA content and metabolism are positively correlated with a faster shoot growth rate of hybrid,and recently,genes participating in both GA biosynthesis and GA response pathways were also found to be differentially expressed between wheat hybrid and its parental inbreds. In this study,an up-regulated GA 20-oxidase gene in a maize hybrid,designated ZmGA20,was cloned. ZmGA20 contains an open reading frame (ORF) encoding 391 amino acid residues. BLASTX searches in GenBank revealed that the ZmGA20 is homologous to the sequences of GA20ox proteins from different species,and analysis also indicated that ZmGA20 had typical features of GA 20-oxidase proteins,including a "LPWKET" sequence. Semi-quantitative RT-PCR analysis showed that ZmGA20 was expressed in different tissues and/or organs. The expression level of ZmGA20 in the hybrid was higher than that in two parents (in roots,leaves,stems and embryo,and ears). The abundance of ZmGA20 transcript was equal to that of the highly expressed parents,which provided molecular evidence for the observed GA content heterosis in maize hybrids.

关键词： GA 20-oxidase Gene expression Maize Heterosis

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时间分辨免疫法检测镇江市内水样中酞酸二丁酯

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江苏大学学报（自然科学版） 2017年第5期38卷 596-602页

作者：朱方柴帅宾金坤杜道林江苏大学环境与安全工程学院江苏镇江212013

酞酸二丁酯(DBP)具有一定的内分泌干扰作用,对环境健康产生不利作用.采用DBP抗体,建立了间接竞争性时间分辨荧光免疫分析方法(TRFIA),测定了镇江市内水域中DBP含量,以风险熵法评估其生态风险.结果显示:优化后的TRFIA方法半数抑制率IC50... 详细信息

酞酸二丁酯(DBP)具有一定的内分泌干扰作用,对环境健康产生不利作用.采用DBP抗体,建立了间接竞争性时间分辨荧光免疫分析方法(TRFIA),测定了镇江市内水域中DBP含量,以风险熵法评估其生态风险.结果显示:优化后的TRFIA方法半数抑制率IC50值为35.8 ng·mL^(-1),最低检测限为2.4 ng·mL^(-1),检测线性范围为4.7~272.6 ng·mL^(-1).该方法特异性高,DBP抗体与7种DBP结构类似物的交叉反应率低于4.65%,加标回收率为79.85%~119.20%,批内差为5.54%~9.93%,批间差为7.27%~14.01%.镇江市内河水中的DBP检出率为100%,质量浓度为5.89~94.57 ng·mL^(-1),河水中DBP分布存在较高的生态风险.

关键词：酞酸二丁酯多克隆抗体荧光免疫分析时间分辨风险熵法

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Modification of LiMn_(0.6)Fe_(0.4)PO_(4)lithium-ion battery cathode materials with a fluorine-doped carbon coating

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Particuology 2024年第9期92卷 278-287页

作者： Debao Pan Ziyuan Liu Chengping Li Rundong Wan Jinsong Wang Jiangzhao Chen Ding Wang jinkun Liu Yingjie Zhang Jianhong Yi Rui Bao Zhengfu Zhang Peng Dong Faculty of Materials Science and Engineering Kunming University of Science and TechnologyKunming 650093China Faculty of Metallurgical and Energy Engineering Kunming University of Science and TechnologyKunming 650093China

In this study,glucose and NH4F were utilized as sources of carbon and fluorine,respectively,for the synthesis of LiMn_(0.6)Fe_(0.4)PO_(4)(LMFP)*** nanoscales were subsequently modified with varying levels of fluorine-doped carbon through co-precipitation and mechanical ball milling *** LMFP,incorporating carbon and varying levels of fluoride ions,exhibit higher specific discharge capacities at 0.2 Cand electrochemical characteristics compared to the original LMFP coated solely with *** inclusion of fluorine-doped carbon in the composite material creates numerous pathways for expeditious electron ***,the partial formation of metal fluoride at the interface between the surface of LMFP and the layer of carbon coating doped with fluorine enhances the reduction in the charge-transfer *** modified ferromanganese phosphate cathode material reveals an outstanding discharge capacity displaying a reversible discharge specific capacity value of 131.73 mA h g^(−1)at 10C and 154.6 mA h g^(−1)at 0.2C,due to its unique structure.

关键词： Lithium manganese iron phosphate Anode Electrochemical Fluorine-doped carbon

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翼缘连接板削弱型上焊下栓钢梁柱节点抗震性能有限元分析

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地震工程学报 2024年第5期46卷 1118-1125页

作者：巴金璐董锦坤辽宁工业大学土木建筑工程学院辽宁锦州121001

分析不同参数对节点抗震性能的影响,为梁柱翼缘连接板削弱型上焊下栓钢结构梁柱节点的设计提供参考。通过改变下翼缘连接板削弱区长度和框架梁截面形状,设计7组算例,利用有限元软件ABAQUS对翼缘连接板削弱型上焊下栓钢梁柱节点进行有限... 详细信息

分析不同参数对节点抗震性能的影响,为梁柱翼缘连接板削弱型上焊下栓钢结构梁柱节点的设计提供参考。通过改变下翼缘连接板削弱区长度和框架梁截面形状,设计7组算例,利用有限元软件ABAQUS对翼缘连接板削弱型上焊下栓钢梁柱节点进行有限元分析,研究其破坏模式、滞回曲线、骨架曲线、最大承载力和螺栓孔径变化率。有限元分析结果表明:下翼缘连接板削弱长度过短无法达到塑性铰外移的目的,削弱长度过长则会导致严重的平面外屈曲;梁全截面削弱组的抗震性能优于半截面削弱组与无变化组。翼缘连接板削弱型上焊下栓钢梁柱节点能有效利用翼缘连接板削弱区的塑性变形、拼接区螺栓的滑移、螺栓杆与孔壁的挤压,以及板件的摩擦来实现耗能。

关键词：钢结构梁柱节点抗震性能有限元分析

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益生菌对肉兔生长性能、养分表观消化率及经济效益的影响

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中国饲料 2024年第10期 35-38页

作者：杨金坤重庆科创职业学院重庆市402160

本试验旨在研究益生菌对肉兔生长性能、养分表观消化率及经济效益的影响。试验将160只健康无病的7周龄伊拉兔随机分为4组,每组5个重复,每个重复8只(公母各一半)。4组肉兔均参考《肉兔营养需要量》(NY/T 4049-2021)配制基础日粮。其中,... 详细信息

本试验旨在研究益生菌对肉兔生长性能、养分表观消化率及经济效益的影响。试验将160只健康无病的7周龄伊拉兔随机分为4组,每组5个重复,每个重复8只(公母各一半)。4组肉兔均参考《肉兔营养需要量》(NY/T 4049-2021)配制基础日粮。其中,对照组饲喂清水,其余3组分别在清水中添加0.5、1.0、1.5 mL/kg的益生菌。预饲期1周,正试期4周。试验期间各组肉兔均自由采食、自由饮水。结果:(1)与对照组相比,1.0 mL/kg益生菌组肉兔末重显著提高4.89%(P<0.05),平均日增重显著提高8.66%(P<0.05),料重比显著降低7.98%(P<0.05);与对照组相比,0.5、1.0、1.5 mL/kg益生菌组肉兔腹泻率显著降低17.43%、52.19%、30.48%(P<0.05)。(2)与对照组相比,0.5、1.0、1.5 mL/kg益生菌组肉兔的粗蛋白质、粗脂肪表观消化率均显著提高(P<0.05);与对照组相比,1.0 mL/kg益生菌组肉兔的干物质表观消化率显著提高(P<0.05),且1.0、1.5 mL/kg益生菌组肉兔的粗纤维、中性洗涤纤维表观消化率均显著提高(P<0.05)。(3)1.0 mL/kg益生菌组的体重增加量、增重收入、毛利润均显著高于对照组(P<0.05)。根据计算,各组肉兔养殖毛利润分别为4.69、5.75、6.89、5.80元/只。结论:饮水中添加适当水平的益生菌能提高肉兔生长性能、养分表观消化率及经济效益。在本试验条件下,益生菌的适宜添加水平为1.0 mL/kg。

关键词：益生菌肉兔生长性能养分表观消化率经济效益

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