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Science China(Life Sciences) 2022年第12期65卷 2455-2471页

作者： Meihua Xie Ni Ma Na Bai Le yang Xuewei yang Ke-Qin Zhang jinkui yang State Key Laboratory for Conservation and Uilization of Bio-Resources Key Laboratory for Microbial Resources of the Ministry of EducationSchool of Life SciencesYumman UniversityKunming 650091China School of Resource Emvironment and ChemistryChuciong Normal UniversityChnuciong 675000China Yunnan Center for Disease Control and Prevention Kunming 650022China

Predatory fungi possess intricate signal transduction systems that regulate their development and support successful infection of the ***,we characterized three components of the cell wall integrity-controlling pathway,namely protein kinase C(Ao PKC),SLT2-MAPK(Ao SLT2),and SWI6(Ao SWI6),in a representative nematode-trapping fungus Arthrobotrys oligospora,using gene disruption and multi-omics *** phenotypic traits(such as mycelia development,conidiation,stress response,and trap morphogenesis) and metabolic profiles of ΔAopkc and ΔAoswi6 mutants were similar but differed from those of the ΔAoslt2 *** analysis indicated that the genes differentially expressed in the absence of Aoswi6 were involved in DNA replication,repair,and recombination during trap ***,the yeast two-hybrid assay showed that Ao PKC interacted with Ao SWI6,suggesting that in ***,PKC can directly regulate SWI6,bypassing the SLT2signaling ***,our findings deepen our understanding of the regulatory mechanism of asexual development and lifestyle switching in nematode-trapping fungi.

关键词： Arthrobotrys oligospora conidiation stress response trap formation differential expression analysis metabolic profile pathogenicity

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AoATG5 plays pleiotropic roles in vegetative growth,cell nucleus development,conidiation,and virulence in the nematode-trapping fungus Arthrobotrys oligospora

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Science China(Life Sciences) 2022年第2期65卷 412-425页

作者： Duanxu Zhou Yingmei Zhu Na Bai Le yang Meihua Xie Jiangliu yang Meichen Zhu Ke-Qin Zhang jinkui yang State Key Laboratory for Conservation and Utilization of Bio-Resources and Key Laboratory for Microbial Resources of the Ministry of Education Yunnan UniversityKunming 650091China School of Life Sciences Yunnan UniversityKunming 650091China

Autophagy is an evolutionarily conserved process in eukaryotes,which is regulated by autophagy-related genes(ATGs).Arthrobotrys oligospora is a representative species of nematode-trapping(NT)fungi that can produce special traps for nematode *** elucidate the biological roles of autophagy in NT fungi,we characterized an orthologous Atg protein,AoAtg5,in *** found that AoATG5 deletion causes a significant reduction in vegetative growth and conidiation,and that the transcript levels of several sporulation-related genes were significantly downregulated during sporulation *** addition,the cell nuclei were significantly reduced in theΔAoATG5 mutant,and the transcripts of several genes involved in DNA bio-synthesis,repair,and ligation were significantly ***ΔAoATG5 mutants,the autophagic process was significantly impaired,and trap formation and nematocidal activity were significantly *** transcriptome analysis results showed that AoAtg5 is involved in the regulation of multiple cellular processes,such as autophagy,nitrogen metabolism,DNA biosynthesis and repair,and vesicular *** summary,our results suggest that AoAtg5 is essential for autophagy and significantly contributes to vegetative growth,cell nucleus development,sporulation,trap formation,and pathogenicity in ***,thus providing a basis for future studies focusing on related mechanisms of autophagy in NT fungi.

关键词： Arthrobotrys oligospora autophagy-related gene AoATG5 phenotypic traits transcriptome analysis sporulation cell nucleus development

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Urinary albumin excretion rate： a risk factor for retinal hard exudates in macular region in type 2 diabetic patients

中国菌物学会2023年学术年会

作者： Zhu meichen yang jinkui State Key Laboratory for Conservation and Utilization of Bio-Resources and Key Laboratory for Microbial Resources of the Ministry of Education Yunnan University School of Life Sciences Yunnan University

The family of Ste20-related kinases is conserved from yeast to mammals and includes the p21activated kinases(PAKs) and germinal center kinases(GCKs).It is commonly thought to be involved in signal transduction pathways as mitogen-activated protein kinase kinase kinase kinase(MAP4K).Here,we characterized two PAK and three GCK orthologous proteins in a typical nematode-trapping fungus,Arthrobotrys oligospora,which can produce adhesive networks to capture,kill,and consume nematodes when sensing external nematode *** analysis showed that the conservation between PAK proteins was higher than that between GCK ***,the phenotypic analysis revealed that both ?Aopak1 and ?Aogck1 were involved in sporulation and pathogenicity,especially in the ?Aogck1 mutant,where the conidiophores were severely deformed and the mycelial growth,nuclei,autophagy,and hyphal fusion were *** results showed that phosphorylation levels of heat shock 70 kDa protein(Hsp70)and 40S ribosomal protein(Rip40) were significantly different in the ?Aogck1 mutant compared to wild-type(WT) strain,and Yeast 2-hybrid assay showed that they physically interacted with *** addition,the phenotypic traits of ?Aohsp70 mutant was similar to that of ?Aogck1 ***,GO and KEGG enrichment of the transcriptomes between ?Aohsp70 and ?Aogck1 mutant suggested that their differentially expressed genes(DEGs) share many common *** results indicated that Aogck1 participated in sporulation and pathogenicity of *** by directly regulating Hsp70 and *** is different from the previous argument that Ste20 functions as ***,our study provides novel insights into the regulatory mechanism of Ste20-related kinases,which will help us to explore the function of signaling pathway in conidiation and trap formation of nematode-trapping fungi.

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Chinese Medical Journal 2014年第12期127卷 2293-2298页

作者： Wang Shaocheng Lin Siyong Cao Xi Zheng Yuezhong Wang Jinyang Lu Na yang jinkui Department of Endocrinology Beijing Tongren HospitalCapital Medical UniversityBeijng Key Laboratory of Diabetes Research and CareBeijing 100730China Clinical College of Ophthalmology Tianjin Medical UniversityTianjin Eye HospitalTianjin 300070China

Background The various risk factors for retinal hard exudates are still poorly understood in type 2 diabetic *** aim of this study was to determine the association between urinary albumin excretion rate （UAER） and hard exudates in macular region in north Chinese *** A total of 272 patients （272 eyes） were enrolled for this study,including 154 subjects from group 1 （mild hard exudates）,91 subjects from group 2 （moderate hard exudates） and 27 subjects from group 3 （severe hard exudates） confirmed using colour fundus photography,optical coherence tomography （OCT） as well as slit-lamp biomicroscopy with 78 diopter （D） *** participant underwent a comprehensive assessment that included biochemical,clinical characteristics test and detailed ophthalmic ***-way analysis of variance （ANOVA） test and chi-square test were performed to analyze the fasting blood glucose （FBG）,glycated hemoglobin （HbA1c）,total cholesterol （TC）,low density lipoprotein （LDL）,high density lipoprotein （HDL）,triglycerides （TG）,full blood counts,urinary albumin excretion rate （UAER）,blood creatinine （CREA）,duration of diabetes,body mass index （BMI）,systolic blood pressures （SBP） and diastolic blood pressures （DBP） between *** logistic regression analysis was further performed in order to eliminating the possible confounding *** Three groups were matched in terms of age and *** factors which showed significant difference between groups include FBG （P ＜0.001）,HbA1c （P ＜0.001）,LDL （P ＜0.001）,UAER （P ＜0.001）,duration of diabetes （P=0.001）,TC （P=0.005）,SBP （P=0.026）,CREA （P=0.004） and haemoglobin （Hb）（P=0.012）.There was no significant difference between groups for the TG,HDL,DBP,platelet,total white blood cells and *** ordinal Logistic regression analyses,of all the variables,HbA1c,LDL and UAER which were independent risk factor for hard exudates showed a significantly odds ratio of 1.25,3.07,and 1.39,*** were also si

关键词： exudates macular edema albuminuia risk factors diabetes mellitus

Sex differences in association between overweight/obesity and cardiovascular disease among adults with pre-diabetes

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中国循环杂志 2016年第Z1期31卷 -页

作者： Zunsong Hu Jie Cao Zhong Dong Jianxin Li Linong Ji jinkui yang Gang Li Jichun Chen Jianfeng Huang Dongfeng Gu Department of Epidemiology FuwaiHospital Chinese Academy of MedicalSciences and Peking Union MedicalColege Beijing China Institute of Chronic Diseases Controland Prevention Beijing Center for Diseases Controland Prevention Beijing China Peking University People’s Hospital Beijing China Beijing Tongren Hospital CapitalMedicalUniversity Beijing China

Relationship between lipid accumulation product and hypertension, diabetes in Beijing residents

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中国循环杂志 2016年第Z1期31卷 -页

作者： Yuanyuan Shen Jie Cao Zhong Dong Jianxin Li Linong Ji jinkui yang Gang Li Jichun Chen Shufeng Chen Aijuan Ma Jianfeng Huang Dongfeng Gu Department of Epidemiology FuwaiHospital Chinese Academy of MedicalSciences and Peking Union MedicalColege Beijing China Institute of Chronic Diseases Controland Prevention Beijing Center for Diseases Controland Prevention Beijing China Peking University People’s Hospital Beijing China Beijing Tongren Hospital CapitalMedicalUniversity Beijing China

胰岛素分泌的双开关:KCNH6的促胰岛素分泌作用

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中国科学基金 2019年第4期33卷 327-333页

作者：杨金奎首都医科大学附属同仁医院内分泌科北京市糖尿病研究所

以往认为,使用60年的经典降糖老药磺脲类药物作用于体内的胰岛素"开关",即胰岛β细胞去极化钾通道KATP,从而促进胰岛素分泌。我们的研究发现,体内胰岛素分泌还受胰岛β细胞复极化钾通道KCNH6的调节。本项目从一个大型四代糖尿病家系入手... 详细信息

以往认为,使用60年的经典降糖老药磺脲类药物作用于体内的胰岛素"开关",即胰岛β细胞去极化钾通道KATP,从而促进胰岛素分泌。我们的研究发现,体内胰岛素分泌还受胰岛β细胞复极化钾通道KCNH6的调节。本项目从一个大型四代糖尿病家系入手,发现该家系同时存在新生儿低血糖与成年糖尿病。该家系KCNH6钾通道基因突变与新生儿低血糖与成年糖尿病共分离。有趣的是,KCNH6基因敲除(KO)或点突变人源化敲入(KI)小鼠的糖尿病表型特征与该家系糖尿病人一致。经过近10年的研究,揭示了KCNH6对胰岛素分泌至关重要,提出了胰岛素分泌"双开关"控制理论。研究成果发表在2018年末的Cell子刊Cell Reports上。

关键词：胰岛素分泌钾离子通道糖尿病 KCNH6

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从糖尿病眼病探索肾病：思路与研究

中华糖尿病杂志 2017年第8期9卷 476-478页

作者：杨金奎首都医科大学附属北京同仁医院内分泌科 100730

眼睛与肾脏在结构、发育和遗传方面有着惊人的相似性,这表明肾脏疾病和眼部疾病可能是紧密联系在一起的.越来越多的研究发现,慢性肾脏病与糖尿病视网膜病变、年龄相关性黄斑变性、青光眼和白内障相关.此外,视网膜微血管参数已被证明可... 详细信息

眼睛与肾脏在结构、发育和遗传方面有着惊人的相似性,这表明肾脏疾病和眼部疾病可能是紧密联系在一起的.越来越多的研究发现,慢性肾脏病与糖尿病视网膜病变、年龄相关性黄斑变性、青光眼和白内障相关.此外,视网膜微血管参数已被证明可预测慢性肾脏疾病.本文从糖尿病眼病探索肾病诊断的科研思路着手,结合我们团队的工作,向各位同仁做一介绍.

关键词：糖尿病眼病肾病糖尿病视网膜病变年龄相关性黄斑变性慢性肾脏疾病慢性肾脏病微血管参数眼部疾病

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均匀化制度对导线用耐热铝合金电导率的影响

轻金属 2002年第9期 58-61页

作者：孙海安杨金魁李晖东北轻合金有限责任公司黑龙江哈尔滨150060

研究了导线用耐热铝合金 ,对其铸锭进行适当的均匀化处理 ,在保证高强度指标和耐热性的同时 ,可进一步提高电导率。研究表明 :含锆的导线用耐热铝合金经过 50 0℃ 16h的均匀化处理 ,可使制品的强度和耐热性满足标准要求 ,且电导率提高 1... 详细信息

研究了导线用耐热铝合金 ,对其铸锭进行适当的均匀化处理 ,在保证高强度指标和耐热性的同时 ,可进一步提高电导率。研究表明 :含锆的导线用耐热铝合金经过 50 0℃ 16h的均匀化处理 ,可使制品的强度和耐热性满足标准要求 ,且电导率提高 1 4 %IACS。电导率提高与锆从α(Al)中充分析出有关 ,锆以稍大于 1× 10 -8m尺寸的ZrAl3 质点在晶粒内部均匀析出 ,可使材料具有较高的电导率。

关键词：导线耐热铝合金均匀化电导率耐热性

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泥水平衡顶管施工技术和质量控制

山西建筑 2018年第30期44卷 95-96页

作者：杨晋魁太原市市政公用工程质量监督站山西太原030012

泥水平衡顶管施工是顶管施工的一种常用方式。介绍了泥水平衡顶管施工原理和施工技术,从泥水控制和旋转过大方面提出综合防治措施,该研究可以对泥水平衡顶管施工具有一定的参考意义。

关键词：泥水平衡顶管施工技术防治措施