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The Jasmine(Jasminum sambac)Genome Provides Insight into the Biosynthesis of Flower Fragrances and Jasmonates

Genomics, Proteomics & Bioinformatics 2023年第1期21卷 127-149页

作者： Gang Chen Salma Mostafa Zhaogeng lu Ran Du Jiawen Cui Yun Wang Qinggang Liao jinkai lu Xinyu Mao Bang Chang Quan Gan Li Wang Zhichao Jia Xiulian Yang Yingfang Zhu Jianbin Yan Biao Jin College of Horticulture and Plant Protection Yangzhou UniversityYangzhou 225009China College of Bioscience and Biotechnology Yangzhou UniversityYangzhou 225009China Department of Floriculture Faculty of AgricultureAlexandria UniversityAlexandria 21526Egypt Key Laboratory of Plant Functional Genomics of the Ministry of Education Yangzhou UniversityYangzhou 225009China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureKey Laboratory of Synthetic BiologyMinistry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China College of Landscape Architecture Nanjing Forestry UniversityNanjing 210037China Institute of Plant Stress Biology State Key Laboratory of Cotton BiologyDepartment of BiologyHenan UniversityKaifeng 475001China

Jasminum sambac(jasmine flower),a world-renowned plant appreciated for its exceptional flower fragrance,is of cultural and economic importance.However,the genetic basis of its fragrance is largely unknown.Here,we present the first de novo genome assembly of J.sambac with 550.12 Mb(scaffold N50=40.10 Mb)assembled into 13 pseudochromosomes.Terpene synthase(TPS)genes associated with flower fragrance are considerably amplified in the form of gene clusters through tandem duplications in the genome.Gene clusters within the salicylic acid/benzoic acid/theobromine(SABATH)and benzylalcohol O-acetyltransferase/anthocyanin O-hydroxycinnamoyltransferases/anthranilate N-hydroxycinnamoyl/benzoyltransferase/deacetylvindoline 4-O-acetyltransferase(BAHD)superfamilies were identified to be related to the biosynthesis of phenylpropanoid/benzenoid compounds.Several key genes involved in jasmonate biosynthesis were duplicated,causing an increase in copy numbers.In addition,multi-omics analyses identified various aromatic compounds and many genes involved in fragrance biosynthesis pathways.Furthermore,the roles of JsTPS3 in b-ocimene biosynthesis,as well as JsAOC1 and JsAOS in jasmonic acid biosynthesis,were functionally validated.The genome assembled in this study for J.sambac offers a basic genetic resource for studying floral scent and jasmonate biosynthesis,and provides a foundation for functional genomic research and variety improvements in Jasminum.

关键词： Jasmine flower Jasminum sambac Genome Jasmonate Flower fragrance

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Copper coordination-driven self-assembly and encapsulation of PCR reagents

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Chinese Chemical Letters 2024年第3期35卷 189-192页

作者： Chang lu jinkai Zheng Juewen Liu Institute of Food Science and Technology Chinese Academy of Agricultural SciencesBeijing 100193China Department of Chemistry Waterloo Institute for NanotechnologyUniversity of WaterlooWaterlooONN2L 3G1Canada

Polymerase chain reactions(PCR)are a very important tool for use in cloning,nucleic acid sequencing and diagnostic testing.The storage conditions of PCR reagents are limited to freezing and a lot of mixing steps are needed.In this paper,we report using metal ions to form coordination nanomaterials with the intrinsic components of the PCR reagents including dNTP,DNA primers and DNA polymerase as an integrated PCR reaction system.To complete PCR reactions,users need only to dissolve the coordination nanomaterials with a buffer and add template DNA.A few transition metal ions were screened and Cu^(2+)was found to be the most effective metal ion for this purpose.Then the encapsulation efficiency of PCR reagents was measured,which can reach close to 100%for the primers and DNA polymerase,but only 10%for dNTP because dNTP was excess.Further study also exhibited this integrated PCR reaction system can be used for DNA detection with a similar detection limit to the normal PCR,and showed good stability of encapsulated PCR nanomaterial after storage for a week.

关键词： Metal ions Coordination Encapsulation PCR Storage

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The genome of Medicago polymorpha provides insights into its edibility and nutritional value as a vegetable and forage legume

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Horticulture Research 2021年第1期8卷 565-576页

作者： Jiawen Cui Zhaogeng lu Tianyi Wang Gang Chen Salma Mostafa Hailong Ren Sian Liu Chunxiang Fu Li Wang Yingfang Zhu jinkai lu Xiang Chen Zhenwu Wei Biao Jin College of Horticulture and Plant Protection Yangzhou University225009 YangzhouChina College of Animal Science and Technology Yangzhou University225009 YangzhouChina Novogene Bioinformatics Institute 100083 BeijingChina College of Bioscience and Biotechnology Yangzhou University225009 YangzhouChina Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences266101 QingdaoChina State Key Laboratory of Crop Stress Adaptation and Improvement Key Laboratory of Cotton BiologySchool of Life SciencesHenan University475001 KaifengChina

Medicago polymorpha is a nutritious and palatable forage and vegetable plant that also fixes nitrogen.Here,we reveal the chromosome-scale genome sequence of M.polymorpha using an integrated approach including Illumina,PacBio and Hi-C technologies.We combined PacBio full-length RNA-seq,metabolomic analysis,structural anatomy analysis and related physiological indexes to elucidate the important agronomic traits of M.polymorpha for forage and vegetable usage.The assembled M.polymorpha genome consisted of 457.53Mb with a long scaffold N50 of 57.72Mb,and 92.92%(441.83Mb)of the assembly was assigned to seven pseudochromosomes.Comparative genomic analysis revealed that expansion and contraction of the photosynthesis and lignin biosynthetic gene families,respectively,led to enhancement of nutritious compounds and reduced lignin biosynthesis in M.polymorpha.In addition,we found that several positively selected nitrogen metabolism-related genes were responsible for crude protein biosynthesis.Notably,the metabolomic results revealed that a large number of flavonoids,vitamins,alkaloids,and terpenoids were enriched in M.polymorpha.These results imply that the decreased lignin content but relatively high nutrient content of M.polymorpha enhance its edibility and nutritional value as a forage and vegetable.Our genomic data provide a genetic basis that will accelerate functional genomic and breeding research on M.polymorpha as well as other Medicago and legume plants.

关键词： forage breeding value

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Numerical Simulation of Rainfall-induced Xianchi Reservoir Landslide in Yunyang,Chongqing,China

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Acta Geologica Sinica(English Edition) 2024年第2期98卷 505-517页

作者： YAN jinkai MA Yan LIU Lei WANG Zhihui REN Tianxiang Chinese Academy of Geological Sciences Beijing 100037China Urban Construction School Beijing City UniversityBeijing 100083China

A calamitous landslide happened at 22:00 on September 1,2014 in the Yunyang area of Chongqing City,southwest China,enforcing the evacuation of 508 people and damaging 23 buildings.The landslide volume comprised 1.44 million m^(3) of material in the source area and 0.4 million m^(3) of shoveled material.The debris flow runout extended 400 m vertically and 1600 m horizontally.The Xianchi reservoir landslide event has been investigated as follows:(1)samples collected from the main body of landslide were carried out using GCTS ring shear apparatus;(2)the parameters of shear and pore water pressure have been measured;and(3)the post-failure characteristics of landslide have been analyzed using the numerical simulation method.The excess pore-water pressure and erosion in the motion path are considered to be the key reasons for the long-runout motion and the scale-up of landslides,such as that at Xianchi,were caused by the heavy rainfall.The aim of this paper is to acquired numerical parameters and the basic resistance model,which is beneficial to improve simulation accuracy for hazard assessment for similar to potentially dangerous hillslopes in China and elsewhere.

关键词： geohazards landslide post-failure rapid and long runout ring shear test

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Recent progress in bio-inspired macrostructure array materials with special wettability—from surface engineering to functional applications

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International Journal of Extreme Manufacturing 2024年第1期6卷 208-256页

作者： Zhongxu Lian Jianhui Zhou Wanfei Ren Faze Chen jinkai Xu Yanling Tian Huadong Yu School of Mechanical and Aerospace Engineering Jilin UniversityChangchun 130022People’s Republic of China Ministry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing Changchun University of Science and TechnologyChangchun 130022People’s Republic of China College of Mechanical and Electrical Engineering Changchun University of Science and TechnologyChangchun 130022People’s Republic of China School of Mechanical Engineering Tianjin UniversityTianjin 300350People’s Republic of China School of Engineering University of WarwickCoventry CV47ALUnited Kingdom

Bio-inspired macrostructure array(MAA,size:submillimeter to millimeter scale)materials with special wettability(MAAMs-SW)have attracted significant research attention due to their outstanding performance in many applications,including oil repellency,liquid/droplet manipulation,anti-icing,heat transfer,water collection,and oil–water separation.In this review,we focus on recent developments in the theory,design,fabrication,and application of bio-inspired MAAMs-SW.We first review the history of the basic theory of special wettability and discuss representative structures and corresponding functions of some biological surfaces,thus setting the stage for the design and fabrication of bio-inspired MAAMs-SW.We then summarize the fabrication methods of special wetting MAAs in terms of three categories:additive manufacturing,subtractive manufacturing,and formative manufacturing,as well as their diverse functional applications,providing insights into the development of these MAAMs-SW.Finally,the challenges and directions of future research on bio-inspired MAAMs-SW are briefy addressed.Worldwide efforts,progress,and breakthroughs from surface engineering to functional applications elaborated herein will promote the practical application of bio-inspired MAAMs-SW.

关键词： macrostructure arrays superhydrophobic 3D printing laser droplet manipulation

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Constant charge method or constant potential method:Which is better for molecular modeling of electrical double layers?

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Journal of Energy Chemistry 2024年第7期94卷 54-60页

作者： Liang Zeng Xi Tan Xiangyu Ji Shiqi Li jinkai Zhang Jiaxing Peng Sheng Bi Guang Feng State Key Laboratory of Coal Combustion Schoolof Energy and PowerEngineeringHuazhong Universityof Science and Technology(HUST)Wuhan 430074HubeiChina Sorbonne Universite CNRSPhysico-chimie des Electrolytes et Nanosystemes InterfaciauxPHENIXF-75005 ParisFrance

In molecular modeling of electrical double layers(EDLs),the constant charge method(CCM)is prized for its computational efficiency but cannot maintain electrode equipotentiality like the more resourceintensive constant potential method(CPM),potentially leading to inaccuracies.In certain scenarios,CCM can yield results identical to CPM.However,there are no clear guidelines to determine when CCM is sufficient and when CPM is required.Here,we conduct a series of molecular simulations across various electrodes and electrolytes to present a comprehensive comparison between CCM and CPM under different charging modes.Results reveal that CCM approximates CPM effectively in capturing equilibrium EDL and current-driven dynamics in open electrode systems featuring ionic liquids or regular concentration aqueous electrolytes,while CPM is indispensable in scenarios involving organic and highly concentrated aqueous electrolytes,nanoconfinement effects,and voltage-driven dynamics.This work helps to select appropriate methods for modeling EDL systems,prioritizing accuracy while considering computationalefficiency.

关键词： Electrochemical interface Molecular dynamics Electrode polarization modeling Nanoconfinement effect

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南海西沙岛礁非饱和珊瑚砂共振柱-弯曲元试验研究

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工程地质学报 2023年第5期31卷 1552-1562页

作者：郭桢蒲建卢劲锴黄雨同济大学土木工程学院地下建筑与工程系中国上海200092 同济大学岩土及地下工程教育部重点实验室中国上海200092

为了确定珊瑚砂的动剪切模量和阻尼比等分析岛礁场地稳定性的主要动力学参数,本文针对我国南海西沙群岛某岛礁珊瑚砂,利用共振柱-弯曲元系统,研究了围压、相对密实度及饱和度对该珊瑚砂动剪切模量和阻尼比的影响规律。研究发现:(1)在饱... 详细信息

为了确定珊瑚砂的动剪切模量和阻尼比等分析岛礁场地稳定性的主要动力学参数,本文针对我国南海西沙群岛某岛礁珊瑚砂,利用共振柱-弯曲元系统,研究了围压、相对密实度及饱和度对该珊瑚砂动剪切模量和阻尼比的影响规律。研究发现:(1)在饱和状态下,该珊瑚砂样的最大动剪切模量(G_(0))与围压及相对密实度整体呈现正相关关系,并且随着围压的增高G_(0)对相对密实度的敏感性逐渐降低;(2)在非饱和情况下,该珊瑚砂样的动剪切模量比的整体范围略高于饱和状态下的动剪切模量比,而其阻尼比略低于饱和状态下的阻尼比;(3)该珊瑚砂样出现最小阻尼比和最大G_(0)的饱和度约为10%;(4)在同样的围压下,珊瑚砂的特殊结构导致建立在硅质砂相对密实度和孔隙比的G_(0)经验预测公式在预测珊瑚砂G_(0)时分别出现了偏高和偏低的情况,本文根据珊瑚砂G_(0)随围压的变化规律,对珊瑚砂G_(0)经验预测公式进行了修正。研究成果可为南海西沙岛礁珊瑚砂场地稳定性分析中动力学参数取值提供参考。

关键词：珊瑚砂共振柱-弯曲元饱和度动剪切模量阻尼比

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树木萌蘖发生的机理研究进展

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植物生理学报 2023年第5期59卷 803-816页

作者：徐源陆金凯刘思安王莉扬州大学园艺与植物保护学院江苏扬州225009

萌蘖起源于腋芽、潜伏芽及不定芽,在树木应对衰老及外界干扰中发挥重要作用。本文对萌蘖的分类和起源,影响萌蘖发生的内在因子如激素、糖信号、树龄等,以及外部环境因子包括光、树木的损伤程度等进行了综述。此外,本文重点阐述了近年来... 详细信息

萌蘖起源于腋芽、潜伏芽及不定芽,在树木应对衰老及外界干扰中发挥重要作用。本文对萌蘖的分类和起源,影响萌蘖发生的内在因子如激素、糖信号、树龄等,以及外部环境因子包括光、树木的损伤程度等进行了综述。此外,本文重点阐述了近年来取得的在调控树木萌蘖形成的关键基因鉴定和分子调控机制方面的研究进展,并对今后的研究方向进行了展望,以期为树木发育研究提供参考。

关键词：树木萌蘖生理分子机制

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An epidermal electrode based triboelectric walking energy harvester for wearable wireless sensing applications

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Science China(Technological Sciences) 2024年第3期67卷 949-957页

作者： CHANG ShiYuan LIU FuHai CHEN jinkai XIA LianBin ZHOU Hao JIANG JingYang DONG Kang ZHANG ChenHao WU Yun CHEN Jin XUAN Weipeng SUN LingLing JIN Hao DONG ShuRong luO JiKui Ministry of Education Key Laboratory of RF Circuits and Systems Hangzhou Dianzi UniversityHangzhou 310018China Special Equipment College Hangzhou Vocational&Technical CollegeHangzhou 310018China College of Information Science and Electronic Engineering Zhejiang UniversityHangzhou 330027China ZJU-Hangzhou Global Scientific and Technological Innovation Center Zhejiang UniversityHangzhou 311200China

Harvesting bio-kinetic energy using a triboelectric nanogenerator(TENG) is one of the promising routes to solve the sustainable energy supply problem for wearable electronics. However, additional materials, complex fabricating processes or specific mechanical structures are needed for existing TENGs to harvest bio-kinetic energy. Besides, they need to be tightly attached to the human body, which may result in detachment and malfunction under tense human motion. Herein, an intrinsic epidermal electrode-based TENG(E-TENG) is proposed to harvest human walking energy. The wearing shoes and ground are used as tribo-materials, and the human epidermis is used as the back electrode of the E-TENG. Compared with the traditional TENGs,the E-TENG does not need any additional tribo-materials and complex mechanical structures. Under optimal conditions, the voltage output of E-TENG can reach 914 V. E-TENG has been used as a self-powered warning sensor and pedometer sensor for demonstration. Furthermore, E-TENG based self-powered wireless sensor system has been developed using a newly designed micro energy electronic switch(MEES). Ambient ultraviolet intensity, temperature and humidity information can be monitored and then transmitted to mobile phone every 3.5 min, demonstrating great potential for widespread wearable applications.

关键词： triboelectric nanogenerator wearable wireless sensing self-powered

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“X不比/没有/不如Y Z”变体选择的多变量分析

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当代语言学 2024年第1期26卷 84-98页

作者：张进凯兴义民族师范学院文学与传媒学院

本研究基于语料库标注数据,首先探讨制约“X不比/没有/不如YZ”变体选择的显著因素及其影响效应,发现当语域偏向书面语、比较项和被比项为无生、结论项极性体现为积极义、比较点出现时,结构选择上倾向于“X没有YZ”“X不如YZ”,但是当... 详细信息

本研究基于语料库标注数据,首先探讨制约“X不比/没有/不如YZ”变体选择的显著因素及其影响效应,发现当语域偏向书面语、比较项和被比项为无生、结论项极性体现为积极义、比较点出现时,结构选择上倾向于“X没有YZ”“X不如YZ”,但是当比较项和被比项词长不等时,结构选择上倾向于“X不比YZ”。本研究发现“X不比/没有/不如YZ”变体选择是多个因素互相作用的结果,而且不同因素之间常常呈现出复杂的多重交互效应。最后发现对“X不比/没有/不如YZ”变体选择具有显著影响的预测变量,按照其相对重要性由高到低依次是:结论项极性、语域、比较点的位置、被比项的生命度、比较项和被比项的词长、比较项的生命度。

关键词：逻辑回归对数优势比条件推断决策树随机森林多变量分析

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