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Phenolic Composition Analysis and Gene Expression in Developing Seeds of Yellow-and Black-seeded Brassica napus

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Journal of Integrative Plant Biology 2013年第6期55卷 537-551页

作者： jinjin jiang Yanlin Shao Aimin Li Chunliang Lu Yongtai Zhang Youping Wang jiangsu Provincial Key Laboratory of Crop Genetics and Physiology Yangzhou University jiangsu Institute of Agricultural Science in the Lixiahe District Test Center of Yangzhou University

Breeders have focused on yellow-seeded Brassica napus （rapeseed） for its better quality com- pared with the black-seeded variety. Moreover, flavonoids have been associated with this kind of rapeseed. In this study, we applied lipid chromatography-electrospray ionization mass spec- trometry （LC-ESI-MSn ） to compare flavonoids in developing seeds of natural black-seeded B. na- pus and yellow-seeded introgression lines selected from progenies of B. napus–Sinapis alba so- matic hybrids. Aside from the most abundant phenolic compounds （sinapine and sinapic acid） and 1, 2-disinapoylglucose, 16 different flavonoids were identified and quantified, including （-）- epicatechin, five monocharged oligomers of （-）-epicatechin （[DP 2]- , [DP 3]- , [DP 4] [DP 2]- B2 and [DP 2]- B5）, quercetin, kaempferol, isorhamnetin-dihexoside, kaempferol-sinapoyl-trihexoside, isorhamnetin- sinapoyl-trihexoside, isorhamnetin-hexoside-sulfate, and isorhamnetin-3-O-glucoside. Most of the flavonoids accumulated with seed development, whereas some rapidly decreased during maturation. The content of these flavonoids was lower in the yellow-seeded materials than in the black seeds. In addition, variations of insoluble procyanidin oligomers and soluble phenolic acids were observed among both rapeseed varieties. Transcriptome changes of genes participating in the flavonoid pathway were discovered by quantitative reverse transcription polymerase chain reaction analysis. Consistent with flavonoid changes identified by high performance liquid chromatography analysis, the expression of most genes in the flavonoid biosynthetic pathway was also downregulated.

关键词： Brassica napus flavonoids gene expression seed development yellow seed.

NAC转录因子调控植物生长发育和胁迫应答的研究进展

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植物生理学报 2024年第2期60卷 271-283页

作者：陆海芹李娜蒋凯旋王幼平蒋金金扬州大学生物科学与技术学院江苏扬州225009

由于植物具有固着生长的特性,其在生长发育过程中往往面临多种来自环境的不利影响。因此,植物必须通过不同的调节机制以维持生长发育和应对胁迫的危害。NAC(NAM/ATAF/CUC)是植物中重要的转录因子家族之一,由高度保守的N端和相对多变的C... 详细信息

由于植物具有固着生长的特性,其在生长发育过程中往往面临多种来自环境的不利影响。因此,植物必须通过不同的调节机制以维持生长发育和应对胁迫的危害。NAC(NAM/ATAF/CUC)是植物中重要的转录因子家族之一,由高度保守的N端和相对多变的C端组成,可以影响植物花器官发育、开花时间、叶片衰老、果实成熟、细胞次生壁形成和根系发育等过程,并参与植物对多种生物胁迫和非生物胁迫的响应过程。本文概述了植物中NAC家族成员在生长发育和胁迫应答过程中发挥的功能,为深入解析NAC转录因子的分子调控机制及其应用提供理论依据。

关键词： NAC转录因子生长发育胁迫应答分子调控

Influence of graphene oxide on electrochemical performance of Si anode material for lithium-ion batteries

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Journal of Energy Chemistry 2016年第5期25卷 817-824页

作者： Wenjing Liu jinjin jiang Hao Wang Chunxiao Deng Feng Wang Gongchang Peng Chengdu Institute of Organic Chemistry Chinese Academy of Sciences Graduate University of Chinese Academy of Sciences

We have developed a Si/graphene oxide electrode synthesized via ultrasonication-stirring method under alkaline condition. Scanning electron microscopy(SEM), transmission electron microscope(TEM), EDS dot-mapping and high-resolution transmission electron microscopy(HRTEM) results show that Si particles are evenly dispersed on the graphene oxide sheets. The electrochemical performance was investigated by galvanostatic charge/discharge tests at room temperature. The results revealed that Si/graphene oxide electrode exhibited a high reversible capacity of 2825 mAh/g with a coulombic efficiency of 94.6%at 100 mA/g after 15 cycles and a capacity retention of 70.8% after 105 cycles at 4000 mA/g. These performance parameters show a great potential in the high-performance batteries application for portable electronics, electric vehicles and renewable energy storage.

关键词： Si/graphene oxide Electrochemical performance High-performance batteries Capacity retention

Three dimensional nano-LiMn_(0.6)Fe_(0.4)PO_4@C/CNT as cathode materials for high-rate lithium-ion batteries

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Journal of Energy Chemistry 2018年第2期27卷 544-551页

作者： Huan Zhang Zhikai Wei jinjin jiang Lei Wang Qi Wan Tao Chen Meizhen Qu Mianzhong Chen Chengdu Institute of Organic Chemistry Chinese Academy of Sciences

Three dimensional（3D） nano-LiMn0.6Fe0.4PO4@C/CNT composite was successfully synthesized by an oleylamine-assisted solvothermal method. The prepared composite showed excellent electrochemical performance, especially superior high rate capability. It could deliver a specific discharge capacity of103.1 mAh/g, even at 80 C. The superior high rate performance of the as-prepared LiMn0.6Fe0.4PO4@C/CNT electrode is attributed to its unique 3D conducting network：（1） the prepared LiMn0.6Fe0.4PO4@C active particles were in nano-scale with a size of 30-50 nm;（2） LiMn0.6Fe0.4PO4 nanoparticles were uniformly coated by amorphous carbon with a thickness of 3 nm;（3） the graphitized conductive CNTs were dispersed homogenously among the LiMn0.6Fe0.4PO4@C active particles. The synergistic effect of the nanoscale amorphous carbon coated LiMn0.6Fe0.4PO4@C active particles and the graphitized CNTs reduces the diffusion path of the lithium ions and benefits the transference ability of electron.

关键词： LiMn0.6 Fe0.4 PO4 Carbon nanotubes Nanoparticles Oleylamine-assisted

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两种生活型木本植物叶功能性状变异特征研究

沈阳农业大学学报 2024年第3期55卷 298-305页

作者：周会萍姜金矜黄艳丽庄静静新乡学院土木工程与建筑学院河南新乡453003

以乔木和灌木两种生活型木本植物为研究对象,研究其植物叶性状及其变异特征,揭示两种生活型木本植物的适应机理。以新乡市16种木本植物:刺槐(Robinia pseudoacacia)、五角枫(Acer elegantulum)、加拿大杨(Populus×ca⁃nadensis)、毛白杨... 详细信息

以乔木和灌木两种生活型木本植物为研究对象,研究其植物叶性状及其变异特征,揭示两种生活型木本植物的适应机理。以新乡市16种木本植物:刺槐(Robinia pseudoacacia)、五角枫(Acer elegantulum)、加拿大杨(Populus×ca⁃nadensis)、毛白杨(Populus tomentosa)、法桐(Platanus orientalis)、白蜡(Fraxinus chinensis)、玉兰(Magnolia denudata)、银杏(Ginkgo biloba)、栾树(Koelreuteria paniculata)、日本晚樱(Cerasus serrulata)、紫荆(Cercis chinensis)、紫叶李(Prunus cerasifera)、垂丝海棠(Malus Halliana)、西府海棠(Malus micromalus)、榆叶梅(Amygdalus triloba)和丁香(Syzygium aromaticum)为试材,对其叶面积(leaf area,LA)、叶厚度(leaf thickness,LT)、叶干物质含量(leaf dry matter content,LDMC)、比叶面积(specific leaf area,SLA)、叶鲜重(fresh leaf weight,FW)、叶干重(dry leaf weight,DW)、叶绿素含量(chlorophyll,Chl)和叶含水率(leaf moisture content,LWC)这8种叶功能性状进行测定和分析。结果表明:8种叶性状存在不同程度的变异。乔木树种的叶鲜重(90.13%)、叶干重(86.36%)和叶面积(95.13%)呈强变异,叶干物质含量(18.79%)和叶含水率(12.81%)属于弱变异;灌木树种的比叶面积(54.03%)、叶鲜重(60.17%)和叶面积(62.17%)属于强变异;叶含水率(14.60%)属于弱变异。叶功能性状间关系密切,乔木树种的叶面积与叶鲜重和叶干重呈极显著正相关关系(p<0.01),与比叶面积呈显著正相关关系(p<0.05);灌木树种的叶面积与叶鲜重、叶干重、比叶面积和叶含水率都呈极显著正相关关系(p<0.01),与叶干物质含量呈极显著负相关关系(p<0.01)。主成分分析显示,8种叶功能性状指标中,叶鲜重、叶干重、叶绿素含量贡献率最大,可作为木本植物适应生境的重要指标。总体来说,乔木树种的大多叶功能性状的稳定性和性状间的相关性与灌木植物相比较弱,乔木树种为适应生态环境,在生存机制上做出的调整程度大于灌木树种。

关键词：生活型木本植物叶功能性状变异

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珠海市南水镇金龙边坡变形破坏特征及稳定性评价

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城市地质 2024年第1期19卷 20-28页

作者：赖波江金进江山江宁赵风顺广东省珠海工程勘察院广东珠海519000

边坡稳定性分析评价是边坡调查评价过程中一个不可或缺的环节。以珠海市金龙边坡为研究对象,分析了边坡的发育特征、影响因素、形成机制。在查明边坡岩土体出露及结构特征基础上,根据典型断面C、ϕ值反演分析及敏感性分析结果,确定金龙... 详细信息

边坡稳定性分析评价是边坡调查评价过程中一个不可或缺的环节。以珠海市金龙边坡为研究对象,分析了边坡的发育特征、影响因素、形成机制。在查明边坡岩土体出露及结构特征基础上,根据典型断面C、ϕ值反演分析及敏感性分析结果,确定金龙边坡岩土体物理力学参数,采用极限平衡法对金龙边坡进行稳定性分析评价。评价结果表明:金龙边坡在天然工况下处于欠稳定状态,在饱和及饱和+地震工况下处于不稳定状态。

关键词：金龙边坡岩质顺层边坡发育特征变形破坏稳定性评价珠海市

Ecological niches and blood sources of sand fly in an endemic focus of visceral leishmaniasis in Jiuzhaigou,Sichuan,China

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Infectious Diseases of Poverty 2016年第1期5卷 288-293页

作者： Huiying Chen Kaili Li Hua Shi Yong Zhang Yu Ha Yan Wang jinjin jiang Yubin Wang Zhenzhou Yang Jiannong Xu Yajun Ma Department of Tropical Infectious Diseases Faculty of Tropical Medicine and Public HealthSecond Military Medical UniversityShanghai 200433China Biology Department Molecular Biology ProgramNew Mexico State UniversityLas CrucesNM 88003USA Center for Disease Control and Prevention of PLA Beijing 100071China Jiuzhaigou Center of Disease Control and Prevention JiuzhaigouSichuan 623400China Center of Disease Control and Prevention General Equipment Department of PLABeijing 100101China

Background:Sand fly Phlebotomus chinensis is a principle vector for the visceral leishmaniasis(VL)in China with a wide geographic distribution.Jiuzhaigou,Sichuan is a mountain type endemic area of VL in China.Long term effective control efforts in the region have successfully reduced VL transmission.To assess the current status of the sand flies and their ecological aspects in the region,a survey was conducted in the summer of 2014 and 2015.Methods:Sand fly specimens were collected by light traps in a village and blood sources were identified by PCR and sequencing of the mitochondrial cytochrome b gene.Results:In a rock cave,65.2%–79.8%of collected sand flies were male.On a rabbit farm,92.9%–98.8%of specimens were female.In pig pens,61.1%of specimens were female.Some females had visible blood residues.The feeding rate was 49.4%from the pig pens,12.3%from the cave,and only 1.7%from the rabbit farm.Pig,rabbit,chicken,dog,and human blood were detected in the fed specimens.Swine blood,present in all tested samples,was a preferred blood source,while chicken and dog blood were present in a third of the samples.Conclusions:In Jiuzhaigou County,Sichuan Province of China,the considerable sandfly density and the peridomestic feeding behavior all increases the risk of VL transmission,and insecticide spraying in animal sheds could be exploited to reduce sand fly populations in human surroundings.

关键词： Sand fly Phlebotomus chinensis Ecological niche Blood sources China

具有抗氧化活性青稞酒糟蛋白酶解液对H_(2)O_(2)诱导HepG2细胞的保护作用

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现代食品科技 2024年第3期40卷 28-38页

作者：陈丽花陈少华荣玉芝冯声宝张叶挺钟研威江津津上海应用技术大学香料香精化妆品学部上海201418 青海互助天佑德青稞酒股份有限公司青海海东810500 上海崇明致富酿造有限公司上海202158 广州城市职业学院食品科学与美食养生学院广东广州510405

为实现青稞酒糟的高值化利用,该研究在提取青稞酒糟蛋白基础上,以DPPH自由基清除率和还原力为指标优化了青稞酒糟蛋白酶解液的制备工艺,并对该酶解液的体外抗氧化活性和细胞保护作用进行了探究。结果表明:青稞水酒糟和青稞白酒糟均含有... 详细信息

为实现青稞酒糟的高值化利用,该研究在提取青稞酒糟蛋白基础上,以DPPH自由基清除率和还原力为指标优化了青稞酒糟蛋白酶解液的制备工艺,并对该酶解液的体外抗氧化活性和细胞保护作用进行了探究。结果表明:青稞水酒糟和青稞白酒糟均含有丰富的蛋白质,含量分别为31.46 g/100 g和18.47 g/100 g;超声纤维素酶法辅助碱提酸沉法对青稞水酒糟的蛋白提取率高达76.10%(m/m),白酒糟的为29.88%;碱性蛋白酶最适合用于青稞水酒糟蛋白的酶解,其最佳酶解条件为:料液比1:60(m/V),超声时间15 min,酶添加量3.7%(m/m)及酶解时间3.5 h,此条件下制得的青稞水酒糟蛋白酶解液的DPPH自由基清除率高达95.02%;不同浓度的青稞水酒糟蛋白酶解液对HepG2细胞活力没有负面影响;随着青稞水酒糟蛋白酶解液浓度的升高,对经H_(2)O_(2)诱导的细胞内ROS逐渐降低,酶解液浓度为5.0 mg/mL(m/V)时可使受损细胞内的ROS比损伤组低约50%。该研究成果可为青稞水酒糟蛋白酶解液的制备及其在保健食品、化妆品中的高值化利用提供参考。

关键词：青稞酒糟蛋白酶解液抗氧化活性细胞活性

Morphology and Mechanical Characteristics of Monolayer and Multilayer Ir Coating by Double Glow Plasma

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Journal of Wuhan University of Technology(Materials Science) 2017年第1期32卷 190-196页

作者：吴王平 jiang jinjin CHEN Zhaofeng jiang Peng WANG Zhizhi School of Mechanical Engineering Changzhou University College of Material Science and Technology Nanjing University of Aeronautics and Astronautics

Multilayer iridium coating was manufactured on tungsten carbide substrates by a double glow plasma process.As comparison,monolayer was also produced.The microstructure and morphology were observed using scanning electron microscopy.Grain orientation and phase were determined using X-ray diffraction.The residual stress of the coating was studied by glancing incidence X-ray diffraction.The adhesive force of the coating was measured by a scratch tester.The results showed that both monolayer and multilayer had a polycrystalline phase with a strong（110） reflection.The coating had an excellent adhesion with no evidence of delamination.The adhesive force of the monolayer and multilayer was about 50 and 43 N,respectively.The interfacial reaction between the substrate and the layer occurred and a new WIr phase was found due to the high-temperature deposition process.The residual stress in the monolayer and multilayer was-1.6 and-1.1 GPa,respectively.

关键词： morphology residual stress adhesive force monolayer multilayer iridium