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Journal of wuhan University of Technology(Materials Science) 2024年第3期39卷 728-737页

作者：何子明申爱琴 WANG Xiaobin wu jinhua WANG Lusheng School of Civil and Architectural Engineering Liaoning University of TechnologyJinzhou 121001China School of Highway Chang’an UniversityXi’an 710064China Department of Road and Bridge Engineering Liaoning Provincial College of Communica-tionsShenyang 110122China

The modification methods of pozzolan slurry combined with sodium silicate and silicon-based additive were respectively adopted to treat recycled coarse brick-mixed aggregate(RCBA)in this study.The compressive strength and chloride permeability resistance of recycled aggregate concrete(RAC)before and after modification treatment were tested,and the microstructure of RAC was analyzed by mercury intrusion porosimetry(MIP)and scanning electron microscopy(SEM).The results show that the physical properties of RCBA strengthened by modification treatment are improved,and the compressive strength and chloride permeability resistance of treated RAC are also significantly improved.The modification treatment optimizes the pore size distribution of RAC,which increases the number of gel pores and transition pores,and decreases the number of capillary pores and macro pores.The surface fractal dimension shows a significant correlation with chloride diffusion coefficient,indicating that the variation of chloride permeability of treated RAC is consistent with the microstructure evolution.

关键词： recycled aggregate concrete modification treatment compressive strength chloride permeability resistance microstructure

Self-optimized single-nanowire photoluminescence thermometry

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Light(Science & Applications) 2023年第2期12卷 259-271页

作者： Zhang Liang jinhua wu Ying Cui Hao Sun Cun-Zheng Ning Department of Electronic Engineering Tsinghua University100084 BeijingChina College of Integrated Circuits and Optoelectronic Chips Shenzhen Technology University518118 ShenzhenGuangdongChina

Nanomaterials-based photoluminescence thermometry(PLT)is a new contact-free photonic approach for temperature sensing,important for applications ranging from quantum technology to biomedical imaging and diagnostics.Even though numerous new materials have been explored,great challenges and deficiencies remain that hamper many applications.In contrast to most of the existing approaches that use large ensembles of rare-earth-doped nanomaterials with large volumes and unavoidable inhomogeneity,we demonstrate the ultimate size reduction and simplicity of PLT by using only a single erbium-chloride-silicate(ECS)nanowire.Importantly,we propose and demonstrate a novel strategy that contains a self-optimization or "smart"procedure to automatically identify the best PL intensity ratio for temperature sensing.The automated procedure is used to self-optimize key sensing metrics,such as sensitivity,precision,or resolution to achieve an all-around superior PLT including several record-setting metrics including the first sensitivity exceeding 100%K^(-1)(~138%K^(-1)),the highest resolution of 0.01 K,and the largest range of sensible temperatures 4-500 K operating completely within 1500-1800 nm(an important biological window).The high-quality ECS nanowire enables the use of well-resolved Stark-sublevels to construct a series of PL intensity ratios for optimization in infrared,allowing the completely Boltzmann-based sensing at cryogenic temperature for the frst time.Our single-nanowire PLT and the proposed optimization strategy overcome many existing challenges and could fundamentally impact PL nano-thermometry and related applications such as single-cell thermometry.

关键词： optimization exceeding simplicity

Crystal structure of Lamellipodin implicates diverse functions in actin polymerization and Ras signaling

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Protein & Cell 2013年第3期4卷 211-219页

作者： Yu-Chung Chang Hao Zhang Mark LBrennan jinhua wu Department of Developmental Therapeutics Fox Chase Cancer Center333 Cottman AvenuePhiladelphiaPA 19111USA

The adapter protein Lamellipodin(Lpd)plays an important role in cell migration.In particular,Lpd mediates lamellipodia formation by regulating actin dynamics via interacting with Ena/VASP proteins.Its RA-PH tandem domain confi guration suggests that like its paralog RIAM,Lpd may also mediate particular Ras GTPase signaling.We determined the crystal structures of the Lpd RA-PH domains alone and with an N-terminal coiled-coil region(cc-RA-PH).These structures reveal that apart from the anticipated coiled-coil interaction,Lpd may also oligomerize through a second intermolecular contact site.We then validated both oligomerization interfaces in solution by mutagenesis.A fluorescence-polarization study demon-strated that Lpd binds phosphoinositol with low affinity.Based on our crystallographic and biochemical data,we propose that Lpd and RIAM serve diverse functions:Lpd plays a predominant role in regulating actin polymerization,and its function in mediating Ras GTPase signaling is largely suppressed compared to RIAM.

关键词： Lamellipodin crystal structure RIAM coiledcoil oligomerization

基于理论承载性能与应力扩散计算的高强预应力锚索适配垫板设计方法

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铁道科学与工程学报 2024年第5期21卷 1942-1953页

作者：郭新新高新强吴锦花成都理工大学环境与土木工程学院四川成都610059 石家庄铁道大学河北省交通工程结构力学行为演变与控制重点实验室河北石家庄050043

垫板是高强预应力锚索系统的核心组成构件,当其与锚索间适配性不足时,会出现结构可靠性低以及围岩支护效果差等问题。基于垫板的承载力学特性及其应力扩散作用,提出适配高强预应力锚索的垫板设计方法。为此,首先分析垫板的应力扩散作用... 详细信息

垫板是高强预应力锚索系统的核心组成构件,当其与锚索间适配性不足时,会出现结构可靠性低以及围岩支护效果差等问题。基于垫板的承载力学特性及其应力扩散作用,提出适配高强预应力锚索的垫板设计方法。为此,首先分析垫板的应力扩散作用与锚索的支护效应间的关联性。其次,基于不同形式垫板的结构受力特征,提出碗形垫板和平板形垫板的理论承载力计算公式,并由此计算得到满足高强预应力锚索承载性能需求的垫板型式及其结构参数。接着,通过建立“垫板(碗形、平板形)-围岩”三维数值仿真模型,分析不同型式及结构参数垫板的围岩应力扩散效果,并由此优选得到适配高强预应力锚索的垫板型式及其结构参数。最后,通过现场应用案例,分析适配垫板下高强预应力锚索系统支护效果。研究结果表明:提出的垫板设计方法实现了对高强预应力锚索系统中垫板的有效选用,适配加载300 kN的高强预应力锚索系统垫板为25 cm×25 cm×16 mm的碗形垫板,其拱高35 mm、孔口半径32 mm、碗底半径75 mm;同时,适配垫板下高强预应力锚索系统的围岩变形控制效果提升了11.9%~15.2%。研究结果有助于提升高强预应力锚索系统的可靠性与支护效果,并为预应力锚固系统中垫板的设计提供了一种可行且有效的方法。

关键词：高强预应力锚索承载性能应力扩散碗形垫板平板垫板

Insights into the electrochemical degradation of sulfamethoxazole and its metabolite by Ti/SnO_(2)-Sb/Er-PbO_(2) anode

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Chinese Chemical Letters 2020年第10期31卷 2673-2677页

作者： Yanping Wang Chengzhi Zhou jinhua wu Junfeng Niu School of Environment and Energy South China University of TechnologyGuangzhou 510006China Research Center for Eco-Environmental Engineering Dongguan University of TechnologyDongguan 523808China

Electrochemical degradation of sulfamethoxazole(SMX)and its metabolite acetyl-sulfamethoxazole(AcSMX)by Ti/SnO_(2)-Sb/Er-PbO_(2) were investigated.Results indicated that the electrochemical degradation of SMX and Ac-SMX followed pseudo-first-order kinetics.The rate constants of SMX and Ac-SMX were 0.268 and 0.072 min-1 at optimal curre nt density of 10 and 14 mA/cm^(2),respectively.Transformation products of SMX and Ac-SMX were identified and the possible degradation pathways,including the cleavage of S-N bond,opening ring of isoxazole and nitration of amino group,were proposed.Total organic carbon removal of SMX was nearly 63.2%after 3 h electrochemical degradation.22.4%nitrogen of SMX was trans formed to NO_(3)-,and 98.8%sulfur of SMX was released as SO_(4)2-.According to quantitative structureactivity relationship model,toxicities of SMX and Ac-SMX to aquatic organisms significantly decreased after electrochemical degradation.Electric energy consumption for 90%SMX and Ac-SMX degradation was determined to be 0.58-8.97 and 6.88-44.19 Wh/L at different experimental conditions,respectively.Compared with parent compound SMX,the metabolite Ac-SMX is more refractory and toxic,which emphasizes the importance of taking its metabolites into account when investigating the disposal of pharmaceuticals from wastewater.

关键词： Electrochemical degradation Sulfamethoxazole and metabolite Degradation mechanism Quantitative structure-activity relationship

Des=gn of efficient simplified genomic DNA and bisulfite sequencing in large plant populations

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Frontiers of Electrical and Electronic Engineering in China 2016年第3期4卷 226-239页

作者： jinhua wu Zewei Luo Ning Jiang Institute of Biostatistics Fudan University Shanghai 200438 China

The next generation sequencing enables generation of high resolution and high throughput data for structure sequence of any genome at a fast declining cost. This opens opportunity for population based genetic and genomic analyses. In many applications, whole genome sequencing or re-sequencing is unnecessary or prohibited by budget limits. The Reduced Representation Genome Sequencing （RRGS）, which sequences only a small proportion of the genome of interest, has been proposed to deal with the situations. Several forms of RRGS are proposed and implemented in the literature. When applied to plant or crop species, the current RRGS protocols shared a key drawback that a significantly high proportion （up to 60%） of sequence reads to be generated may be of non-genomic origin but attributed to chloroplast DNA or rRNA genes, leaving an exceptional low efficiency of the sequencing experiment. We recommended and discussed here the design of optimized simplified genomic DNA and bisulfite sequencing strategies, which may greatly improves efficiency of the sequencing experiments by bringing down the presentation of the undesirable sequencing reads to less than 10% in the whole sequence reads. The optimized RAD- seq and RRBS-seq methods are potentially useful for sequence variant screening and genotyping in large plant/crop populations.

关键词： plant/crop genomes next generation sequencing genotyping restriction-enzyme sites associated DNA（RAD） DNA methylation reduced representation bisulfite sequencing

Hybrid microbial electrolytic/UV system for highly efficient organic pollutants removal

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Journal of Environmental Sciences 2019年第9期31卷 39-45页

作者： Yingying You Shaobin Huang Xuesong Zhao Han Li Fangqin Cheng jinhua wu Yongqing Zhang Shaofeng Zhou School of Environment and Energy South China University of Technology Guangzhou 510006 China School cf Environmental Science and Engineering Guangdong University of Technology Guangzhou 510006 China Department of Environmental Engineering Technical University cf Denmark DK-2800 Lyngby Denma Institute of Resources and Environmental Engineering Shanxi University Taiyuan 030006 China Guangzhou Taihe Water Ecology Technology Co. Ltd. Guangzhou 510220 China

This study for the first time proposed an efficient microbial electrolyte/UV system for Methyl Orange decomposition. With an external applied voltage of 0.2 V and cathode aeration of20 mL/min, H2O2 could be in-situ generated from two-electron reduction of oxygen in cathode, reaching to 8.1 mg/L in 2 hr and continued to increase. The pollutant removal efficiency of approximate 94.7% was achieved at initial neutral pH, with the activation of ·OH in the presence of UV illumination. Although the nature of its guiding principles remain on the vista of practical exploration, this proof-of-concept study provides an alternative operation pattern of solar–microbial hybrid technology for future wastewater treatment from a basic but multidisciplinary view.

关键词： Microbial electrolytic cell Hydrogen peroxide production Photo-Fenton reaction Organic pollutants removal Advanced oxidation process

个人数字记忆世界的“身后事”:社交媒体用户网络数字遗产立嘱意愿影响因素

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图书馆论坛 2024年第1期44卷 116-123页

作者：吴金华石燕青是沁南京农业大学信息管理学院南京中医药大学卫生经济管理学院

网络时代承载越来越多的个人数字足迹、痕迹和印记,引发人们对自身网络数字遗产是“记忆”还是“被遗忘”的思考。文章以社交媒体用户网络数字遗产问题为研究考量,基于数字原住民视角,运用扎根理论的质性研究方法对访谈资料进行三级编... 详细信息

网络时代承载越来越多的个人数字足迹、痕迹和印记,引发人们对自身网络数字遗产是“记忆”还是“被遗忘”的思考。文章以社交媒体用户网络数字遗产问题为研究考量,基于数字原住民视角,运用扎根理论的质性研究方法对访谈资料进行三级编码分析,理清主范畴间的作用机制,构建社交媒体用户网络数字遗产立嘱意愿的UIPEI模型分析框架。研究发现:用户维、数据信息维和社交媒体平台维直接影响社交媒体用户的网络数字遗产立嘱意愿,接受主体维和外在环境维间接影响社交媒体用户的网络数字遗产立嘱意愿;可从用户认知、平台服务和政策法规等强化网络数字遗产保护和个人数字记忆传承。

关键词：数字遗产网络数字遗产社交媒体用户数字原住民个人数字记忆

“共同富裕”:递嬗脉络、逻辑架构与话语指向——基于党的文献的文本考梳

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云南行政学院学报 2024年第2期26卷 12-23页

作者：吴金花南京理工大学马克思主义学院

实现共同富裕,是中国共产党领导人民进行中国式现代化建设的坚定奋斗目标。以党的文献为中心,对“共同富裕”的用法进行文本考梳,可以探查“共同富裕”话语体系的递嬗脉络、逻辑结构和话语指向。从时间坐标轴来看,在党的文献中,“共同... 详细信息

实现共同富裕,是中国共产党领导人民进行中国式现代化建设的坚定奋斗目标。以党的文献为中心,对“共同富裕”的用法进行文本考梳,可以探查“共同富裕”话语体系的递嬗脉络、逻辑结构和话语指向。从时间坐标轴来看,在党的文献中,“共同富裕”的最初使用是在1953年,主要用于“农业生产”领域,改革开放后确立了“先富带动后富”的目标,新时代以来“共同富裕”逐步扩展到了各个领域。从空间坐标轴来看,中共对“共同富裕”的使用,既有顶层设计层面的概念,又有关键领域和微观实践方面的概念。从指向上看,“共同富裕”在叙事意涵、叙事机理、叙事结构等方面特征鲜明。

关键词：中国共产党党的文献共同富裕

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化工项目介质毒性分类方法及使用场合探讨

化工与医药工程 2024年第1期45卷 3-7页

作者：吴金华神华工程技术有限公司北京100011

当前化工行业介质毒性判定规范较多且各成体系。为合理划分化工项目介质毒性,文章介绍了化工项目介质毒性三种分类方式,并对三种分类方式涉及的规范以及规范之间的关系进行分析,明确不同毒性分类方法的使用场合,供化工项目设计工作参考... 详细信息

当前化工行业介质毒性判定规范较多且各成体系。为合理划分化工项目介质毒性,文章介绍了化工项目介质毒性三种分类方式,并对三种分类方式涉及的规范以及规范之间的关系进行分析,明确不同毒性分类方法的使用场合,供化工项目设计工作参考使用。

关键词：毒性分类危害程度急性毒性化工项目