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Revealing the specific regulations of nitric oxide on the postharvest ripening and senescence of bitter melon fruit

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aBIOTECH 2024年第1期5卷 29-45页

作者： Hongwei Wang Ling Li Lili Ma Alisdair R.Fernie Anzhen fu Chunmei Bai Zhaoze Sang Susu Guo Fan Zhang Qing Wang Yanyan Zheng jinhua Zuo Key Laboratory of Vegetable Postharvest Processing Ministry of AgricultureBeijing Key Laboratory of Fruits and Vegetable Storage and ProcessingKey Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China)of Ministry of AgricultureKey Laboratory of Urban Agriculture(North)of Ministry of AgricultureBeijing Vegetable Research CenterInstitute of Agro-Products Processing and Food NutritionBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China College of Food Science and Biotechnology Tianjin Agricultural UniversityTianjin 300392China Max Planck Institute of Molecular Plant Physiology 14476 Potsdam GolmGermany Institute of Food Science and Technology Chinese Academy of Agricultural SciencesBeijingChina

Bitter melon fruit is susceptible to yellowing,softening,and rotting under room-temperature storage conditions,resulting in reduced commercial value.Nitric oxide(NO)is an important signaling molecule and plays a crucial role in regulating the fruit postharvest quality.In this study,we investigated the effects of NO treatment on changes in sensory and firmness of bitter melon fruit during postharvest storage.Moreover,transcriptomic,metabolomic,and proteomic analyses were performed to elucidate the regulatory mechanisms through which No treatment delays the ripening and senescence of bitter melon fruit.Our results show that differentially expressed genes(DEGs)were involved in fruit texture(CSLE,β-Gal,and PME),plant hormone signal transduction(ACS,JAR4,and AUX28),and fruit flavor and aroma(SUS2,LOX,and GDH2).In addition,proteins differentially abundant were associated with fruit texture(PLY,PME,and PGA)and plant hormone signal transduction(PBL15,JAR1,and PYL9).Moreover,No significantly increased the abundance of key enzymes involved in the phenylpropanoid biosynthetic pathway,thus enhancing the disease resistance and alleviating softening of bitter melon fruit.Finally,differential metabolites mainly included phenolic acids,terpenoids,and flavonoids.These results provide a theoretical basis for further studies on the physiological changes associated with postharvest ripening and senescence of bitter melon fruit.

关键词： Bitter melon NO treatment Postharvest Ripening and senescence

Skin Toxicity of Wufang Babu Ointment to Zebrafish

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Medicinal Plant 2024年第2期15卷 40-42,47页

作者： Xiaoxia LI Jiabao MA Jing fu jinhua LIU Xiongbin GUI The First Affiliated Hospital of Guangxi University of Chinese Medicine Nanning 530023China

[Objectives]Using wild-type AB strain of zebrafish as experimental animal,this study investigated the damaging effect of Wufang Babu Ointment on skin cells,in order to evaluate the skin toxicity of Wufang Babu Ointment.[Methods]Wild-type AB strain of zebrafish with an age of 2 d were taken and fed in different concentrations of Wufang Babu Ointment solution for 24 h.The number of deaths in each group of zebrafish was recorded,and the mortality rate was calculated.Using Origin 8.0 software,the maximum non lethal concentration(MNLC)was simulated.Zebrafish raised in different concentrations of Wufang Babu Ointment solution for 24 h were placed under an anatomical microscope for taking photos,to analyze and calculate the incidence of skin damage in zebrafish.Based on the statistical analysis results of this indicator,the skin toxicity of Wufang Babu Ointment was evaluated.[Results]The MNLC of Wufang Babu Ointment on zebrafish was 671μg/mL;Wufang Babu Ointment can induce skin damage at the concentrations of 224μg/mL(1/3 MNLC)and 671μg/mL(MNLC).[Conclusions]Wufang Babu Ointment had certain skin toxicity to zebrafish.

关键词： Wufang Babu Ointment Zebrafish Skin toxicity

Gas accumulation conditions and key technologies for exploration & development of Sulige gasfield

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Petroleum Research 2018年第2期3卷 91-109页

作者： jinhua fu Liyong Fan Xinshe Liu Daojun Huang PetroChina Changqing Oilfield Company Shaanxi710018China Research Institute of Petroleum Exploration and Development PetroChina Changqing Oilfield CompanyShaanxi710018China Exploration Department PetroChina Changqing Oilfield CompanyShaanxi710018China National Engineering Laboratory of Low Permeability Oil and Gas Field Exploration and Development Shaanxi710018China

Up to now,the Sulige area in Ordos Basin has the favorable exploration area of 55×10^(3) km^(2),the total reserve of natural gas of nearly 6×10^(12) m^(3) and the proven reserve(including basic proven reserve)of 4.77×10^(12) m^(3),where the annual production of natural gas reaches 23×10^(9) m^(3),and the Sulige gasfield is the largest onshore natural gas field in China.The pay zone of the Sulige gasfield mainly is Member 8 of Shihezi Formation and Member 1 of Shanxi Formation of Permian which belong to the typical tight sandstone gas reservoir.The coal measure strata in Carboniferous Benxi Formation,Permian Taiyuan Formation and Shanxi Formation provide abundant gas sources for the Gulige gas reservoirs.An open-flow sedimentary model of lacustrine delta is developed,the gentle bottom,sand supply from multisource,strong hydrodynamic force and multi-period superposition control the distribution of largearea reservoir sand body.Lithology of the reservoir is the sandstone of the fluvial-delta facies,the physical property is poor and the heterogeneity is strong,the average porosity ranges from 4%to 12%and the average permeability varies from 0.01 to 1 mD.The gas reservoir is characterized by wide hydrocarbon generation,pervasive hydrocarbon charging,short-range migration and massive accumulation.The pressure coefficient of the gas reservoir ranges from 0.62 to 0.90,indicating the low-pressure gas reservoir,and the single-well yield is low.full digital seismic technique in the desert area,nonlongitudinal seismic technique in the loess plateau,accurate logging evaluation technique,tight sand reservoir stimulation technology and horizontal well development technology are key technologies for exploration and development of Sulige gasfield.

关键词： Sulige gasfield Upper paleozoic Tight gas Accumulation condition Key technology

Determination of Benzoylaconitine Compounds in the Decoctions of Aconiti Lateralis Radix Praeparata(Heishun Pieces),Trichosanthis Fructus and Their Combination

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Medicinal Plant 2024年第3期15卷 1-3,22页

作者： Luyao XIE Xiaoxia LI Jing fu jinhua LIU Yaobin HUANG Xuri WEI Tingting MO Shaomian PAN Lin HUANG Jiabao MA Graduate School of Guangxi University of Chinese Medicine Nanning 530200China The First Affiliated Hospital of Guangxi University of Chinese Medicine Nanning 530023China

[Objectives]This study was conducted to determine the contents of benzoylmesaconine,benzoylaconitine and benzoylhypacoitine in the decoctions of Heishun pieces,Trichosanthis Fructus and their combination.[Methods]Heishun pieces,Trichosanthis Fructus and their combination were extracted for different time periods,and then grouped.HPLC was performed using an Agilent ZORBAX SB-C 18 chromatographic column(4.6 mm×250 mm,5μm)and acetonitrile-0.02 mol/L sodium dihydrogen phosphate as the mobile phase at a flow rate of 1 mL/min and a column temperature of 30℃,and the sample volume was 20μL.The detection wavelength was 230 nm.[Results]The total amounts of benzoylmesaconine,benzoylaconitine and benzoylhypacoitine in the single decoction group of Heishun pieces were all significantly different from those in the combined decoction group at corresponding time.[Conclusions]The total content of the benzoylaconitine type increased significantly after the combined decoction of Heishun pieces and Fructus Trichosanthis,which proves the scientificity of"eighteen incompatible medicaments,19 counteraction"in traditional Chinese medicine to some extent.

关键词： Aconiti Lateralis Radix Praeparata Trichosanthis Fructus Benzoylaconitine compound Content determination

Reservoir formation conditions and key technologies for exploration and development in Qingcheng large oilfield

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Petroleum Research 2020年第3期5卷 181-201页

作者： Suotang fu jinhua fu Xiaobing Niu Shixiang Li Zhiyu Wu Xinping Zhou Jiangyan Liu National Engineering Laboratory for Exploration and Development of Low-Permeability Oil&Gas Fields Xi’an City710018China PetroChina Changqing Oilfield Company Xi’an City710018China No.11 Oil Production Plant PetroChina Changqing Oilfield CompanyXifeng745000China China University of Petroleum(East China) Qingdao266580China Exploration and Development Research Institute PetroChina Changqing Oilfield CompanyXi’an710018China

The Qingcheng oilfield,the largest shale oil field in China,was discovered in 2019 in source rock of Chang 7 Member of Mesozoic Yanchang Formation,Changqing Oilfield in Ordos Basin,with newly increased proven geological reserves of 358 million tons,predicted geological reserves of 693 million tons,and a total of 1.051 billion tons of shale oil resources.This achieves a historic breakthrough in exploration of shale oil in Chang 7 Member.In recent years,focusing on key issues,such as whether there develop sweet spots in source rock,whether commercial oil production capacity can be formed,and whether scale-effective development can be achieved,studies about geological conditions for shale oil accumulation have been continuously carried out,key supporting technologies have been developed,and a series of theoretical innovations and technological breakthroughs have been achieved in oil exploration in source rocks.The results indicate that shale oil accumulation in Chang 7 Member is controlled by following factors.Firstly,lacustrine black shale,dark mudstone and other high-quality source rocks lay a material foundation for shale oil formation.Secondly,sandy deposits intercalated within black shale and dark mudstones are exploration sweet spots,and sandbody combination types are controlled by morphology of bottom of the lacustrine basin.Thirdly,reservoir properties are good due to development of micro-nano pore throats.Finally,reservoirs with high oil saturation and high gas-oil ratio are formed due to high-intensity oil charging in source rock.Effective matching of multiple factors is a key for shale oil accumulation in Chang 7 Member in Ordos Basin.Through innovation of key supporting technologies,exploration and development technologies for shale oil are developed,and substantial breakthrough on exploration and development of shale oil is realized.The first application of logging-seismic combination technology in loess plateau improves significantly quality of 3D seismic data,which effectively guides prediction of san

关键词： Chang 7 member of Yanchang formation Shale oil Geological conditions for oil accumulation Supporting technologies for exploration and development Qingcheng oilfield Ordos basin

Shuganjieyu capsule increases neurotrophic factor expression in a rat model of depression

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Neural Regeneration Research 2014年第5期9卷 489-497页

作者： jinhua fu Yingjin Zhang Renrong Wu Yingjun Zheng Xianghui Zhang Mei Yang Jingping Zhao Yong Liu Mental Health Institute of the Second Xiangya Hospital Key Laboratory of Psychiatry and Mental Health of Hunan ProvinceCentral South University Department of Psychiatry Brain Hospital of Hunan Province Laboratory of Ethnopharmacology Institute of Integrated Traditional Medicine and Western Medicine the Second Xiangya Hospital Central South University Guangzhou Brain Hospital Affiliated Hospital of Guangzhou Medical College

Shuganjieyu capsule has been approved for clinical treatment by the State Food and Drug Ad-ministration of China since 2008. In the clinic, Shuganjieyu capsule is often used to treat mild to moderate depression. In the rat model of depression established in this study, Shuganjieyu capsule was administered intragastrically daily before stress. Behavioral results conifrmed that depressive symptoms lessened after treatment with high-dose （150 mg/kg） Shuganjieyu capsule. Immunohistochemistry results showed that high-dose Shuganjieyu capsule signiifcantly increased phosphorylation levels of phosphorylation cyclic adenosine monophosphate response element binding protein and brain-derived neurotrophic factor expression in the medial prefrontal cortex and hippocampal CA3 area. Overall, our results suggest that in rats, Shuganjieyu capsule effec-tively reverses depressive-like behaviors by increasing expression levels of neurotrophic factors in the brain.

关键词： nerve regeneration Shuganjieyu capsule depression neurotrophic factor brain-derived neurotrophic factor phosphorylation cyclic adenosine monophosphate response element binding pro- tein chronic unpredictable mild stress NSFC grant neural regeneration

Graphitic carbon nitride nanosheets mitigate cadmium toxicity in Glycine max L.by promoting cadmium retention in root and improving photosynthetic performance

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Journal of Environmental Sciences 2024年第5期139卷 543-555页

作者： Kai Xu Yunyu Guo Chenghua Xing Ronglong fu Bin Zou Rongchuan Liu Luyi Cai Jianfang Yan Xi-Lin Wu Miaozhen Cai College of Geography and Environmental Sciences Zhejiang Normal UniversityJinhua 321004China College of Agriculture Jinhua PolytechnicJinhua 321007China

Cadmium(Cd)pollution poses a serious threat to plant growth and yield.Nanomaterials have shown great application potential for alleviation of Cd toxicity to plants.In this study,we applied graphitic carbon nitride nanosheets(g-C_(3)N_(4)NSs)for alleviation of Cd-toxicity to soybean(Glycine max L.).The g-C_(3)N_(4)NSs supplementation significantly improved plant growth and reduced oxidative damage in the Cd-toxicated soybean seedlings through hydroponic culture.Particularly,the g-C_(3)N_(4)NSs dynamically regulated the root cell wall(RCW)components by increasing pectin content and modifying its demethylation via enhancing pectin methylesterase(PME)activity,therefore greatly enhanced stronger RCW-Cd retention(up to 82.8%)and reduced Cd migration to the shoot.Additionally,the g-C_(3)N_(4)NSs reversed the Cd-induced chlorosis,increased photosynthetic efficiency because of enhancement in F_v/F_mration,Y(Ⅱ)and sugars content.These results provide new insights into the alleviation of Cd toxicity to plants by g-C_(3)N_(4)NSs,and shed light on the application of low-cost and environmental-friendly carbon-based NMs for alleviating heavy metal toxicity to plants.

关键词： Cadmium Carbon nitride nanosheets Cell wall Pectin Pectin methylesterase Soybean

Immunological disturbance effect of exogenous histamine towards key immune cells

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Food Science and Human Wellness 2024年第4期13卷 1856-1863页

作者： Yanan Liu Huan Li Chong Wang Shanjun Chen Renjie Lian Weiqiang Wang Linglin fu Yanbo Wang Food Safety Key Laboratory of Zhejiang Province School of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China Jinghai Group Co. Ltd.Rongcheng 264307China jinhua Jinnian Ham Co. Ltd.Jinhua 321041China School of Food and Health Beijing Technology and Business UniversityBeijing 100048China

Histamine in food has attracted widespread attention due to the potential toxicity and associated health risk.However,its influences on immunological components,especially the function of key immune cells,are still poorly known.In this work,we explored the effects of exogenous histamine on the function of key immune cells such as intestinal epithelial cells,dendritic cells,and T cells.The results showed that histamine could affect the expression of allergy-related genes in CMT93 cells at a high dose of histamine.Moreover,it’s found that histamine could cause an imbalance in the levels of relevant immune factors secreted by dendritic cells and T cells,especially those related to allergy.At the same time,the proportion of MHC class IIpositive dendritic cells and the proportion of T helper 2(Th2)cells in CD4^(+)T cells increased after histamine stimulation.We concluded that the presence of a certain level of histamine in food may affect the expression of allergy-related cytokines,disrupt the balance of the immune homeostasis,and potentially lead to adverse immune reactions.This work demonstrated the importance of including the estimation of histamine’s immune safety in aquatic products rather than merely considering the potential risk of food poisoning.

关键词： Histamine Immunological disturbance Food allergy Immune factor Edible safety

Comparison of immune responses and intestinal flora in epicutaneously sensitized BALB/c or C57BL/6 mouse models of food allergy

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Food Science and Human Wellness 2024年第2期13卷 668-680页

作者： Gang Yu Yuhao Jiang Shuifeng Zhang Pengpeng Liu Shunyu Wang Huadong Sheng Yanbo Wang Qiaozhi Zhang Linglin fu Food Safety Key Laboratory of Zhejiang Province School of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China Zhejiang Fangyuan Test Group Co. Ltd.Hangzhou 310018China Key Laboratory of Biosafety Detection for Zhejiang Market Regulation Hangzhou 310018China Zhejiang Liziyuan Food Co. LtdJinhua 321031China

Cutaneous exposure to food allergens through a disrupted skin barrier is recognized as an important cause of food allergy,and the cutaneous sensitized mouse model has been established to investigate relevant allergic disorders.However,the role of different genetic backgrounds of mice on immune responses to food allergens upon epicutaneous sensitization is largely unknown.In this study,two strains of mice,i.e.,the BALB/c and C57BL/6 mice,were epicutaneously sensitized with ovalbumin on atopic dermatitis(AD)-like skin lesions,followed by intragastric challenge to induce IgE-mediated food allergy.Allergic outcomes were measured as clinical signs,specific antibodies and cytokines,and immune cell subpopulations,as well as changes in intestinal barrier function and gut microbiota.Results showed that both strains of mice exhibited typical food-allergic symptoms with a Th2-skewed response.The C57BL/6 mice,rather than the BALB/c mice,were fitter for establishing an epicutaneously sensitized model of food allergy since a stronger Th2-biased response and severer disruptions in the intestinal barrier and gut homeostasis were observed.This study provides knowledge for selecting an appropriate mouse model to study food-allergic responses associated with AD-like skin lesions and highlights the role of genetic variations in the immune mechanism underlying pathogenesis of food allergy.

关键词： Food allergy Mouse models Epicutaneous sensitization Th2 response Gut microbiota