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Food Science and Human Wellness 2024年

作者： Yaxi Zhou Diandian Wang Hao Duan Shiqi Zhou jinhong guo Yu guo Jian Zhao Wenjie Yan Beijing Key Laboratory of Bioactive Substances and Functional Food， College of Biochemical Engineering， Beijing Union University

The anti-inflammatory properties of silkworm pupa peptide (SPP) have been recognized for their potential benefits in colitis treatment. However， the underlying mechanism of SPP in colitis animal models remains unclear. The objective of our work was to investigate the improving effect and the mechanism of action of SPP on a mouse model of dextrose sodium sulfate (DSS)-induced colitis. The findings suggested that SPP effectively eased ulcerative colitis (UC) symptoms in mice by decreasing disease activity index， ameliorating histopathological injury， and decreasing serum and colonic markers of inflammation. In addition， our research findings demonstrated that SPP restored intestinal barrier function by increasing the production of tight junction proteins such as zonula occludens-1 (ZO-1)， claudin-1， claudin-3， occludin， and adherens junction protein E-cadherin1. Further， SPP supplementation enhanced the concentration of short-chain fatty acids and positively altered the makeup of the gut bacteria in the mice’s gut. These findings underscore SPP’s ability to slow the progression of colitis and point to its possible use as a functional component in dietary supplements for the prevention of early-stage colitis.

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基于金包覆磁性纳米探针的多模态LFIA检测胃癌标志物

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西南大学学报（自然科学版） 2024年第6期46卷 29-39页

作者：程杰袁浩岚严家圣郭九川郭劲宏上海交通大学感知科学与工程学院上海200240 成都理工大学计算机与网络安全学院(示范性软件学院) 成都610059 电子科技大学信息与通信工程学院成都611731

多模态侧流免疫层析(Lateral Flow Immunoassay,LFIA)技术能够提供不同灵敏度水平的多种检测模式,极大地提高了即时检测的实用性和灵活性,已迅速发展成为一个极具潜力的研究方向.研究中合成了一种金包裹的超顺磁性纳米晶簇(SMNC@Au)多... 详细信息

多模态侧流免疫层析(Lateral Flow Immunoassay,LFIA)技术能够提供不同灵敏度水平的多种检测模式,极大地提高了即时检测的实用性和灵活性,已迅速发展成为一个极具潜力的研究方向.研究中合成了一种金包裹的超顺磁性纳米晶簇(SMNC@Au)多功能纳米探针,开发了用于同时检测胃癌标志物胃蛋白酶原(Pepsinogen,PG)Ⅰ和Ⅱ的多模态LFIA传感平台.首先通过水热法合成超顺磁性纳米晶簇,然后通过种子生长法在其上涂覆一层金壳,最后用4-巯基苯甲酸修饰颗粒表面形成最终的SMNC@Au探针.该多功能探针可以提供比色、磁性和表面增强拉曼散射3种输出信号,且能通过磁力实现分析物的分离和富集,简化样本的处理.基于SMNC@Au纳米探针LFIA支持定性比色读数以及磁信号和拉曼信号的两种定量读数,可适应不同的检测场景和要求.检测结果表明,PGⅠ和PGⅡ的视觉检测限分别为10 ng/mL和1 ng/mL;磁性模式的检测限分别为0.5 ng/mL和0.1 ng/mL;拉曼模式的检测限分别为0.1 ng/mL和0.05 ng/mL.两种定量模式的检测范围为1~500 ng/mL(PGⅠ)和0.1~100 ng/mL(PGⅡ),完全符合临床诊断标准.该测定表明,所开发的多模态LFIA在不同环境下灵敏检测分析物均有较大潜力.

关键词：多模态探针多功能纳米材料侧流免疫层析胃蛋白酶原即时检测

Preparation and application of microfluidic SERS substrate:Challenges and future perspectives

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Journal of Materials Science & Technology 2020年第2期37卷 96-103页

作者： Jiuchuan guo Fanyu Zeng jinhong guo Xing Ma School of Information and Communication Engineering University of Electronic Science and Technology of ChinaChengdu 610054China State Key Laboratory of Advanced Welding and Joining Harbin Institute of Technology(Shenzhen)Shenzhen 518055China Flexible Printed Electronic Technology Center School of Materials Science and EngineeringHarbin Institute of Technology(Shenzhen)Shenzhen 518055China

Surface-enhanced Raman spectroscopy(SERS),as a highly sensitive molecular analysis technique,can realize fast and non-destructive detection of the information of molecular bonds to identify the component of analytes by"fingerprint"*** preparation of SERS substrates plays an extremely important role in the development of SERS technology and the application of SERS *** integrating SERS enhancement substrates into microfluidic chips,researchers have developed the microfluidic SERS chips which expand the function of microfluidic chips and provide an efficient platform for on-site biochemical analysis equipped with the powerful sensing capability of SERS *** this paper,we will first briefly give a review of the current microfluidic SERS-active substrates preparation technology and present the perspective on the application prospects of microfluidic SERS-active *** then the challenges in the preparation of microfluidic SERS-active substrates will be pointed out,as well as realistic issues of using this technology for biochemical application.

关键词： SERS substrate Microfluidics Biochemical sensing On-site analysis

Nanomaterial Labels in Lateral Flow Immunoassays for Point-of-Care-Testing

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Journal of Materials Science & Technology 2021年第1期60卷 90-104页

作者： Jiuchuan guo Shuqin Chen jinhong guo Xing Ma School of Information and Communication Engineering University of Electronic Science and Technology of ChinaChengdu 610054China Flexible Printed Electronic Technology Center&School of Materials Science and Engineering Harbin Institute of Technology(Shenzhen)Shenzhen 518055China Shenzhen Bay Laboratory No.9 Duxue RoadShenzhen 518055China

Lateral flow immunoassays(LFIAs) have been developed rapidly in recent years and used in a wide range of application at point-of-care-testing(POCT),where small biomolecules can be conveniently examined on a test *** with other biochemical detection methods such as ELISA(enzyme linked immunosorbent assay) or mass spectrometry method,LFIAs have the advantages of low cost,easy operation and short ***,it suffers from low sensitivity since conventional LFIA can only realize qualitative detection based on colorimetric *** the increasing demand for more accurate and sensitive determination,novel nanomaterials have been used as labels in LFIAs due to their unique advantages in physical and chemical *** gold,fluorescent nano particles,SERSactive nanomaterials,magnetic nanoparticles and carbon nanomaterials are utilized in LFIAs to produce different kinds of signals for quantitative or semi-quantitative *** review paper first gives a description of the LFIA principles,and then focuses on the state-of-the-art nanomaterial labelling technology in *** last,the conclusion and outlook are given to inspire exploration of more advanced nanomaterials for the development of future LFIAs.

关键词： lateral flow immunoassays(LFIA) point-of-care-testing(POCT) nanomaterial labelling technology quantitative detection

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乳清蛋白的功能及其在保健食品中的应用

食品科学 2024年第19期45卷 304-316页

作者：王点点周亚西王鲜春段昊郭晋红郑越闫文杰北京联合大学生物化学工程学院生物活性物质与功能食品北京市重点实验室北京100023 鄂尔多斯市东胜区市场监督管理局内蒙古鄂尔多斯017000

乳清蛋白富含多种生物活性成分,并且已被证明具有增强免疫、抗细菌病毒、抗癌、抗糖尿病、缓解肌肉损失和保护皮肤等多种功能特性。但其作为一种能够促进人体健康的优质膳食蛋白质,在保健食品的应用上却表现出产品种类单一和创新性不足... 详细信息

乳清蛋白富含多种生物活性成分,并且已被证明具有增强免疫、抗细菌病毒、抗癌、抗糖尿病、缓解肌肉损失和保护皮肤等多种功能特性。但其作为一种能够促进人体健康的优质膳食蛋白质,在保健食品的应用上却表现出产品种类单一和创新性不足的特点。因此,本文对乳清蛋白的成分、消化、吸收、代谢、功能及其在我国保健产品上的应用进行较为系统和全面的综述。以期进一步深化大众对乳清蛋白营养价值的认识,拓宽乳清蛋白作为保健产品的适用范围,促进其在功能性食品和临床配方上的应用,从而推动国民健康产业的高质量发展。

关键词：乳清蛋白消化保健产品研究进展

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雌激素受体Esr1诱导红耳龟早期卵巢分化

生物工程学报 2024年第7期40卷 2308-2321页

作者：郭金洪杨洪哲时翔胡颖王圣云汪思凡孙伟葛楚天浙江万里学院动物性别与发育重点研究所浙江宁波315100 浙江万里学院生物与环境学院浙江宁波315100 中国海洋大学海洋生命学院山东青岛266000

为探究3种雌激素受体(estrogen receptor) Esr1、Esr2和Gper1在红耳龟早期胚胎性腺分化中的作用,本研究在分析受体基因表达特征的基础上,通过向性腺分化启动前的产雄温度(male-producing temperature,MPT)龟胚分别注射Esr1、Esr2和Gper... 详细信息

为探究3种雌激素受体(estrogen receptor) Esr1、Esr2和Gper1在红耳龟早期胚胎性腺分化中的作用,本研究在分析受体基因表达特征的基础上,通过向性腺分化启动前的产雄温度(male-producing temperature,MPT)龟胚分别注射Esr1、Esr2和Gper1激动剂PPT、WAY 200070和G-1,从性腺形态结构、生殖细胞分布模式、性别分化关键基因和蛋白表达分布方面对处理后的胚胎性腺进行了性逆转分析。表达分析结果显示,esr1在性别分化关键时期性腺中的表达量显著高于esr2和gper1 (表达极低),且呈现产雌温度(female-producing temperature,FPT)性腺高表达。功能验证实验显示,PPT处理后的MPT性腺形态结构明显雌性化,生殖细胞呈现雌性分布模式;雄性分化关键基因dmrt1、amh和sox9 m RNA表达明显下降,雌性分化关键基因foxl2和cyp19a1 m RNA表达则显著上升;Amh和Sox9蛋白表达的荧光信号几乎消失,Foxl2和Arom蛋白被激活出现大量表达,表明性腺由雄性逆转为雌性(性逆转率:70.27%)。而WAY 200070和G-1处理后的MPT性腺仍分化为睾丸,雌雄基因和蛋白的表达及分布与雄性性腺类似。结果表明在红耳龟中,单独激活Esr1能够充分启动早期性腺的雌性分化过程,提示雌激素可能通过其受体1 (Esr1)诱导早期卵巢分化。本研究为进一步解析雌激素在龟性别决定和分化中的调控机制提供了参考。

关键词：雌激素受体性腺分化雌激素性逆转红耳龟

Liquid Metal-Based Epidermal Flexible Sensor for Wireless Breath Monitoring and Diagnosis Enabled by Highly Sensitive SnS2 Nanosheets

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Research 2021年第1期2021卷 1319-1331页

作者： Yifan Huang Fan Yang Sanhu Liu Rongguo Wang jinhong guo Xing Ma National Key Laboratory of Science and Technology on Advanced Composites in Special Environments Harbin Institute of TechnologyHarbin 150086China Sauvage Laboratory for Smart Materials School of Materials Science and EngineeringHarbin Institute of Technology(Shenzhen)Shenzhen 518055China Shenzhen STRONG Advanced Materials Research Institute Co. Ltd.China School of Communication and Information Engineering University of Electronic Science and Technology of ChinaChengdu 611731China Shenzhen Key Laboratory of Flexible Printed Electronics Technology Harbin Institute of Technology(Shenzhen)Shenzhen 518055China Shenzhen Bay Laboratory No.9 Duxue RoadShenzhen 518055China

Real-time wireless respiratory monitoring and biomarker analysis provide an attractive vision for noninvasive telemedicine such as the timely prevention of respiratory arrest or for early diagnoses of chronic ***,wearable respiratory sensors are in high demand as they meet the requirement of portability in digital healthcare ***,high-performance sensing material plays a crucial role for the precise sensing of specific markers in exhaled air,which represents a complex and rather humid ***,we present a liquid metal-based flexible electrode coupled with SnS_(2)nanomaterials as a wearable gas-sensing device,with added Bluetooth capabilities for remote respiratory monitoring and *** flexible epidermal device exhibits superior skin compatibility and high responsiveness(1092%/ppm),ultralow detection limits(1.32 ppb),and a good selectivity of NO gas at ppb-level *** advantage of the fast recovery kinetics of SnS_(2)responding to H_(2)O molecules,it is possible to accurately distinguish between different respiratory patterns based on the amount of water vapor in the exhaled ***,based on the different redox types of H_(2)O and NO molecules,the electric signal is reversed once the exhaled NO concentration exceeds a certain threshold that may indicate the onset of conditions like asthma,thus providing an early warning system for potential lung ***,by integrating the wearable device into a wireless cloud-based multichannel interface,we provide a proof-of-concept that our device could be used for the simultaneous remote monitoring of several patients with respiratory diseases,a crucial field in future digital healthcare management.

关键词： Highly reversed integrating

Artificial intelligence reinforced upconversion nanoparticle-based lateral flow assay via transfer learning

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Fundamental Research 2023年第4期3卷 544-556页

作者： Wei Wang Kuo Chen Xing Ma jinhong guo School of Information and Communication Engineering University of Electronic Science and Technology of ChinaChengdu 611731China School of Software Engineering Chongqing University of Posts and TelecommunicationsChongqing 400065China School of Materials Science and Engineering Harbin Institute of Technology(Shenzhen)Shenzhen 518055China The M.O.E.Key Laboratory of Laboratory Medical Diagnostics The College of Laboratory MedicineChongqing Medical UniversityChongqing 400016China

The combination of upconverting nanoparticles(UCNPs)and immunochromatography has become a widely used and promising new detection technique for point-of-care testing(POCT).However,their low luminescence efficiency,non-specific adsorption,and image noise have always limited their progress toward practical ***,artificial intelligence(AI)has demonstrated powerful representational learning and generalization capabilities in computer *** report for the first time a combination of AI and upconversion nanoparticle-based lateral flow assays(UCNP-LFAs)for the quantitative detection of commercial internet of things(IoT)*** universal UCNPs quantitative detection strategy combines high accuracy,sensitivity,and applicability in the field detection *** using transfer learning to train AI models in a small self-built database,we not only significantly improved the accuracy and robustness of quantitative detection,but also efficiently solved the actual problems of data scarcity and low computing power of POCT ***,the trained AI model was deployed in IoT devices,whereby the detection process does not require detailed data preprocessing to achieve real-time inference of quantitative *** validated the quantitative detection of two detectors using eight transfer learning models on a small *** AI quickly provided ultra-high accuracy prediction results(some models could reach 100%accuracy)even when strong noise was ***,the high flexibility of this strategy promises to be a general quantitative detection method for optical *** believe that this strategy and device have a scientific significance in revolutionizing the existing POCT technology landscape and providing excellent commercial value in the in vitro diagnostics(IVD)industry.

关键词： Upconverting nanoparticles Lateral flow assays Transfer learning Internet of medical things Portable fluorescent sensor

Effects of encoding and retrieval on the mechanism of item+context binding

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Chinese Science Bulletin 2011年第17期56卷 1787-1798页

作者： XIAO Xin DING jinhong guo ChunYan Beijing Key Laboratory of Learning and Cognition and Department of Psychology Capital Normal University Beijing 100089 China

To investigate the neural mechanism of semantic representation and color context binding,we used electroencephalograph time frequency and coherence analyses to reveal local and long-range functional coupling in the encoding and retrieval phases of episodic *** undergraduates participated in the experiment and middle-frequency double-character Chinese words were used as stimuli in two types of study-test tasks (context recall and context recognition tests).Significant differences between item+context and item in the encoding phase were observed at the electrodes in the frontal region at 600-800 ms by time frequency *** differences were observed at 800 ms by independent component analysis:the frontal component and the coherent component of the triangle phase locking structure among the prefrontal,right parietal and left parietal-occipital *** the retrieval phase,differences between item+context and item were found on the electrodes at the central parietal and parietal-occipital regions at 400 ms by time frequency analysis,on the parietal-occipital component at 800 ms by independent component analysis and on the coherence component of the anterior right hemisphere and parietal-occipital regions at 1400 *** conclusion,the different effects of encoding and retrieval processing on 'binding' are reflected by the differing extents that brain regions engaged in cognitive *** the retrieval phase in particular,activities of the parietal-occipital region were specifically associated with 'binding',and coherence between frontal and temporal-parietal regions is a common brain activity in episodic memory.

关键词：神经机制约束力编码时间频率分析提取独立成分分析组成部分色彩范围

Performance enhancement of paper-based SERS chips by shell-isolated nanoparticle-enhanced Raman spectroscopy

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Journal of Materials Science & Technology 2019年第10期35卷 2207-2212页

作者： Mingze Sun Binghan Li Xiaojia Liu Jiayin Chen Taotao Mu Lianqing Zhu jinhong guo Xing Ma State Key Laboratory of Advanced Welding and Joining Harbin Institute of Technology(Shenzhen)Shenzhen518055China Flexible Printed Electronics Technology Center Harbin Institute of Technology(Shenzhen)Shenzhen518055China Beijing Engineering Research Center of Optoelectronic Information and Instruments Beijing Information Science and Technology UniversityBeijing100016China School of Information and Communication Engineering University of Electronic Science and Technology of ChinaChengdu671731China

Paper-based flexible surface-enhanced Raman scattering(SERS) chips have been demonstrated to have great potential for future practical applications in point-of-care testing(POCT) due to the potentials of massive fabrication, low cost, efficient sample collection and short signal acquisition time. In this work,common filter paper and Ag@Si O2 core-shell nanoparticles(NP) have been utilized to fabricate SERS chips based on shell-isolated nanoparticle-enhanced Raman spectroscopy(SHINERS). The SERS performance of the chips for POCT applications was systematically investigated. We used crystal violet as the model molecule to study the influence of the size of the Ag core and the thickness of the Si O2 coating layer on the SERS activity and then the morphology optimized Ag@Si O2 core-shell NPs was employed to detect thiram. By utilizing the smartphone as a miniaturized Raman spectral analyzer, high SERS sensitivity of thiram with a detection limit of 10^-9 M was obtained. The study on the stability of the SERS chips shows that a Si O2 shell of 3 nm can effectively protect the as-prepared SERS chips against oxidation in ambient atmosphere without seriously weakening the SERS sensitivity. Our results indicated that the SERS chips by SHINERS had great potential of practical application, such as pesticide residues detection in POCT.

关键词： SERS chip Point-of-Care test(POCT) Shell-core nanostructure Stability