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SUPER WOMAN 2(SPW2)maintains organ identity in spikelets by inhibiting the expression of floral homeotic genes OsMADS3,OsMADS58,OsMADS13,and DROOPING LEAF

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Journal of Integrative Agriculture 2024年第1期23卷 59-76页

作者： Hui Zhuang Jinsong Lan Qiuni Yang Xiaoyu zhao Yuhuan Li jingya Zhi Yalin Shen GuanghuaHe Yunfeng Li Rice Research Institute Key Laboratory of Application and Safety Control of Genetically Modified CropsAcademy of Agricultural SciencesSouthwest UniversityChongqing 400715China

Flower organ identity in rice is mainly determined by the A-,B-,C-and E-class genes,with the majority encoding MADS-box transcription factors.However,few studies have investigated how the expression of these floral organ identity genes is regulated during flower development.In this study,we identified a gene named SUPER WOMAN 2(SPW2),which is necessary for spikelet/floret development in rice by participating in the regulation of the expression of pistil identity genes such as OsMADS3,OsMADS13,OsMADS58 and DL.In the spw2 mutant,ectopic stigma/ovary-like tissues were observed in the non-pistil organs,including sterile lemma,lemma,palea,lodicule,and stamen,suggesting that the identities of these organs were severely affected by mutations in SPW2.SPW2 was shown to encode a plant-specific EMF1-like protein that is involved in H3K27me3 modification as an important component of the PRC2 complex.Expression analysis showed that the SPW2 mutation led to the ectopic expression of OsMADS3,OsMADS13,OsMADS58,and DL in non-pistil organs of the spikelet.The ChIP-qPCR results showed significant reductions in the levels of H3K27me3 modification on the chromatin of these genes.Thus,we demonstrated that SPW2 can mediate the process of H3K27me3 modification of pistil-related genes to regulate their expression in non-pistil organs of spikelets in rice.The results of this study expand our understanding of the molecular mechanism by which SPW2 regulates floral organ identity genes through epigenetic regulation.

关键词： rice(Oryza sativa) spikelet organ identity H3K27me3

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A hybrid bacterium with tumor-associated macrophage polarization for enhanced photothermal-immunotherapy

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Acta Pharmaceutica Sinica B 2022年第6期12卷 2683-2694页

作者： jingya zhao Huabei Huang Jinyan zhao Xiang Xiong Sibo Zheng Xiaoqing Wei Shaobing Zhou Key Laboratory of Advanced Technologies of Materials Ministry of EducationSchool of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China

Remodeling the tumor microenvironment through reprogramming tumor-associated macrophages(TAMs) and increasing the immunogenicity of tumors via immunogenic cell death(ICD) have been emerging as promising anticancer immunotherapy strategies.However,the heterogeneous distribution of TAMs in tumor tissues and the heterogeneity of the tumor cells make the immune activation challenging.To overcome these dilemmas,a hybrid bacterium with tumor targeting and penetration,TAM polarization,and photo thermal conversion capabilities is developed for improving antitumor immunotherapy in vivo.The hybrid bacteria(B.b@QDs) are prepared by loading Ag;S quantum dots(QDs) on the Bifidobacterium bifidum(B.b) through electrostatic interactions.The hybrid bacteria with hypoxia targeting ability can effectively accumulate and penetrate the tumor tissues,enabling the B.b to fully contact with the TAMs and mediate their polarization toward M1 phenotype to reverse the immunosuppressive tumor microenvironment.It also enables to overcome the intratumoral heterogeneity and obtain abundant tumor-associated antigens by coupling tumor penetration of the B.b with photo thermal effect of the QDs,resulting in an enhanced immune effect.This strategy that combines B.b-triggered TAM polarization and QD-induced ICD achieved a remarkable inhibition of tumor growth in orthotopic breast cancer.

关键词： Immunotherapy Tumor-associated macrophage Immunogenic cell death Bifidobacterium Quantum dot

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The Controversies and Challenges in the Management of Twin Pregnancy: From the Perspective of International Federation of Gynecology and Obstetrics Guidelines

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Maternal-Fetal Medicine 2022年第4期4卷 255-261页

作者： Jingyu Liu Quanrui Liu jingya zhao Danlun Li Yi Zhou Fetal Medicine Center Department of Obstetrics and GynecologyTheFirst Affliated Hospital of Sun Yat-sen UniversityGuangzhou 510080China

The rate of multiple pregnancy is increasing, mainly because of the widespread use of assisted reproduction techniques and families’ desire for twins. Twin pregnancy accounts for a higher risk of chromosomal abnormalities, structural malformations, and neonatal adverse events than singleton pregnancy. The presence of artery-vein anastomoses, unbalanced placenta sharing, and abnormal cord insertion in monochorionic twins is associated with twin complications such as twin-to-twin transfusion syndrome, selective intrauterine growth restriction, and twin anemia polycythemia sequence. Although many guidelines and studies have established and improved the processes about the antenatal surveillance and management of twin pregnancy, they also raise more controversies and challenges. This review aims to highlight the international consensus on the antenatal care of twin pregnancies and analyze the controversies and predicaments based on the published International Federation of Gynecology and Obstetrics guidelines and research.

关键词： Ultrasonography Pregnancy twin Selective intrauterine growth restriction Intertwin discordance Twin-to-twin transfusion syndrome Twin anemia polycythemia sequence Controversies

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复合磷酸盐螯合剂对超高压处理的骆驼乳品质的影响

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食品与发酵工业 2024年第1期50卷 141-147页

作者：张萌萌赵静雅双全内蒙古农业大学食品科学与工程学院内蒙古呼和浩特010018

骆驼乳有较高的营养价值及保健功效,但超高压灭菌过程中经常出现凝乳现象,因而常加入磷酸盐螯合剂提高骆驼乳的稳定性。该研究以探究超高压处理的骆驼乳品质的影响为目的,以超高压处理后的骆驼乳为研究对象,对不同磷酸盐螯合剂的种类、... 详细信息

骆驼乳有较高的营养价值及保健功效,但超高压灭菌过程中经常出现凝乳现象,因而常加入磷酸盐螯合剂提高骆驼乳的稳定性。该研究以探究超高压处理的骆驼乳品质的影响为目的,以超高压处理后的骆驼乳为研究对象,对不同磷酸盐螯合剂的种类、比例进行感官评定,优化加入磷酸盐螯合剂后超高压处理的工艺,分析加入复合磷酸盐螯合剂的超高压处理骆驼乳的理化指标、流变学特性、感官风味等品质特性产生的影响。结果表明,加入0.07%焦磷酸钠和0.1%(均为质量分数)六偏磷酸钠组成的复合磷酸盐螯合剂的骆驼乳,经550 MPa、22 min超高压处理后,其酸度值降低,pH值升高,流变学特性良好,酸味、苦味和涩味值减弱,且对氮氧化合物、醛酮类及芳香族化合物敏感。综上所述,加入复合磷酸盐螯合剂可改善超高压处理的骆驼乳的品质。该研究为综合利用骆驼乳以及骆驼乳的产业化提供试验基础和理论依据。

关键词：骆驼乳超高压处理磷酸盐螯合剂感官品质

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Progress in NMR-based metabolomics of Catharanthus roseus

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Frontiers of Agricultural Science and Engineering 2015年第3期2卷 195-204页

作者： Qifang PAN jingya zhao Yuliang WANG Kexuan TANG Joint International Research Laboratory of Metabolic&Developmental Sciences Key Laboratory of Urban Agriculture(South)Ministry of AgriculturePlant Biotechnology Research CenterFudan-SJTU-Nottingham Plant Biotechnology R&D CenterSchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China

Metabolomics has been rapidly developed as an important field in plant sciences and natural products chemistry.As the only natural source for a diversity of monoterpenoid indole alkaloids(MIAs),especially the low-abundance antitumor agents vinblastine and vincristine,Catharanthus roseus is highly valued and has been studied extensively as a model for medicinal plants improvement.Due to multistep enzymatic biosynthesis and complex regulation,genetic modification in the MIA pathway has resulted in complicated changes of both secondary and primary metabolism in C.roseus,affecting not only the MIA pathway but also other pathways.Research at the metabolic level is necessary to increase knowledge on the genetic regulation of the whole metabolic network connected to MIA biosynthesis.Nuclear magnetic resonance(NMR)is a very suitable and powerful complementary technique for the identification and quantification of metabolites in the plant matrix.NMR-based metabolomics has been used in studies of C.roseus for pathway elucidation,understanding stress responses,classification among different cultivars,safety and quality controls of transgenic plants,cross talk between pathways,and diversion of carbon fluxes,with the aim of fully unravelling MIA biosynthesis,its regulation and the function of the alkaloids in the plant from a systems biology point of view.

关键词： Catharanthus roseus monoterpenoid indole alkaloids NMR metabolomics

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预混地塞米松的高分子自交联透明质酸水凝胶抑制软骨细胞凋亡缓解早期创伤性骨关节炎的作用机制

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中华医学杂志 2024年第9期104卷 695-703页

作者：张志伟赵静雅冯毅尹崑李鹏翠卫小春山西医科大学第二医院骨科骨与软组织损伤修复山西省重点实验室太原030001 太原市人民医院内分泌科太原030001

目的探究预混有地塞米松(Dex)的高分子自交联透明质酸(cHA)水凝胶(cHA-Dex水凝胶)抑制软骨细胞凋亡缓解早期创伤性骨关节炎(PTOA)的作用机制。方法取2月龄雄性SD大鼠140只分两组,70只进行前交叉韧带切断(ACLT)手术制造PTOA动物模型,以... 详细信息

目的探究预混有地塞米松(Dex)的高分子自交联透明质酸(cHA)水凝胶(cHA-Dex水凝胶)抑制软骨细胞凋亡缓解早期创伤性骨关节炎(PTOA)的作用机制。方法取2月龄雄性SD大鼠140只分两组,70只进行前交叉韧带切断(ACLT)手术制造PTOA动物模型,以假手术组(70只)为对照,在术后不同时间点(1~14 d,每组每个时间点5只大鼠)检测关节灌洗液中白细胞介素1β(IL-1β)、IL-6、肿瘤坏死因子α(TNF-α)、基质金属蛋白酶3(MMP-3)及MMP-13等炎性指标的变化趋势。将cHA-Dex(0.5 mg/ml)水凝胶(实验组,n=70)及预混有Dex的普通低分子透明质酸(HA)水凝胶,即HA-Dex(0.5 mg/ml)水凝胶(对照组,n=70)注入PTOA大鼠关节腔,检测每个时间点(1~14 d,每组每个时间点5只大鼠)大鼠关节液中的Dex的释放量及累计释放量,揭示cHA-Dex水凝胶的缓释机制。取2020年1月至2022年12月在山西医科大学第二医院关节科进行膝关节置换术后的骨关节炎(OA)患者(胫骨平台上Outbridge评分1~2分,n=18)膝关节软骨进行体外组织块培养,将预混有0.1、0.2、0.5 mg/ml三种梯度Dex浓度的cHA-Dex水凝胶处理软骨组织块后,检测关节软骨组织块中IL-1β、IL-6、TNF-α、MMP-3以及MMP-13的mRNA表达水平变化。运用酶解法分离出OA软骨细胞并进行体外原代培养(来自18例OA患者,分6次重复实验,每次3例患者的软骨细胞提取后混合均匀培养),软骨细胞贴壁后分为4组,分别进行下列干预:生理盐水干预组、cHA水凝胶干预组、Dex(0.5 mg/ml)干预组、cHA-Dex(0.5 mg/ml)水凝胶干预组,检测不同干预对软骨细胞增殖及凋亡的影响。结果大鼠PTOA组和假手术组膝关节番红O固绿染色国际骨关节炎协会(OARSI)评分分别为(3.34±0.35)分和(1.17±0.21)分(P=0.010);PTOA大鼠膝关节骨赘Meachim评分相对于假手术组增高[(2.66±0.41)分比(0.22±0.17)分,P=0.010],大鼠PTOA造模成功。与PTOA大鼠关节内炎症因子早期出现峰值变化规律相对应的cHA-Dex水凝胶中Dex也在早期释放,并在后期长期缓释。预混0.1、0.2、0.5 mg/ml Dex的cHA-Dex水凝胶处理OA关节软骨组织块后,组织块中的IL-1β、IL-6、TNF-α、MMP-3及MMP-13的mRNA表达均下降(均P<0.05)。体外培养OA软骨细胞,与Dex(0.5 mg/ml)水凝胶干预组相比,cHA-Dex(0.5 mg/ml)水凝胶干预组凋亡率明显减少(0.60±0.07比6.63±0.98,P=0.010)。相对生理盐水干预组或单纯cHA水凝胶干预组,cHA-Dex(0.5 mg/ml)水凝胶干预组细胞增殖明显,各时间点差异均有统计学意义(均P<0.05)。结论针对PTOA的早期炎症,cHA-Dex水凝胶可能通过缓释Dex,既可抑制软骨炎症,又可逆转Dex导致的软骨细胞凋亡增加、增殖率降低,最终缓解PTOA进展。

关键词：骨关节炎创伤性骨关节炎地塞米松高分子自交联透明质酸水凝胶软骨炎症因子凋亡增殖

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Atrial of arbidol hydrochloride in adults with COVID-19

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Chinese Medical Journal 2022年第13期135卷 1531-1538页

作者： jingya zhao Jinnong Zhang Yang Jin Zhouping Tang Ke Hu Hui Sun Mengmeng Shi Qingyuan Yang Peiyu Gu Hongrong Guo Qi Li Haiying Zhang Chenghong Li Ming Yang Nian Xiong Xuan Dong Juanjuan Xu Fan Lin Tao Wang Chao Yang Bo Huang Jingyi Zhang hi Chen Qiong He Min Zhou Jieming Qu Department of Pulmonary and Critical Care Medicine Rujin HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200025China institute of Respiratory Diseases School of MedicineShanghai Jiao Tong UniversityShanghai 200025China Department of Emergency Union Medical College Affiliated to Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430058China Department of Respiratory and Critical Care Medicine Union Medical Collge Affiliated to Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430058China Department of Neurology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430030China Department of Respiratory and Critical Care Medicine Renmin HospitalWuhan UniversityWuhanHubei 430060China Department of Endocrinology Union Medical Cllege Affiliated to Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430058China Department of Respiratory Medicine Wuhan Bauhinia HospitalWuhanHubei 430062China Department of Respiratory Medicine Guanggu Hospital DistrictThe Third Hospital of WuhanWuhanHubei 430074China Department of Respiratory and Crtical Care Medicin Puren HosptalWuhan University of Science and TechnologyWuhanHubei 430081China Department of Respiratory Medicine The Third People's Hospital of Hubei ProvinceWuhanHubei 430030China Department of Respiatory Medicine The Sixth General Hospital of Hubei ProvinceWuhanHubei 430015China Tuberculosis Department of Chengdu Public Health Clinical Medical Center ChengduSichuan 610066China Department of Neurology Wuhan Red Cross HospitalWuhanHubei 430015China Department of Tuberculosis Jinyintan HospiaWuhanHubei 430048China Department of Cardiology The Third People's Hospital of Hubei ProvinceWuhanHubei 430030China.

Background: To date, there is no effective medicine to treat coronavirus disease 2019 (COVID-19), and the antiviral efficacy of arbidol in the treatment for COVID-19 remained equivocal and controversial. The purpose of this study was to evaluate the efficacy and safety of arbidol tablets in the treatment of COVID-19.Methods: This was a prospective, open-label, controlled and multicenter investigator-initiated trial involving adult patients with confirmed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Patients were stratified 1:2 to either standard-of-care (SOC) or SOC plus arbidol tablets (oral administration of 200 mg per time, three times a day for 14 days). The primary endpoint was negative conversion of SARS-CoV-2 within the first week. The rates and 95% confidential intervals were calculated for each variable.Results: A total of 99 patients with laboratory-confirmed SARS-CoV-2 infection were enrolled;66 were assigned to the SOC plus arbidol tablets group, and 33 to the SOC group. The negative conversion rate of SARS-CoV-2 within the first week in patients receiving arbidol tablets was significantly higher than that of the SOC group (70.3% [45/64]vs. 42.4% [14/33];difference of conversion rate 27.9%;95% confidence interval [CI], 7.7%-48.1%;P=0.008). Compared to those in the SOC group, patients receiving arbidol tablets had a shorter duration of clinical recovery (median 7.0 daysvs. 12.0 days;hazard ratio [HR]: 1.877, 95% CI: 1.151-3.060,P=0.006), symptom of fever (median 3.0 daysvs. 12.0 days;HR: 18.990, 95% CI: 5.350-67.410,P 0.05). The most common adverse event in the arbidol tablets group was the elevation of transaminase (5/200, 2.5%), and no one withdrew from the study due to adverse events or disease progression.Conclusions: SOC plus arbidol tablets significant

关键词： Arbidol Coronavirus disease 2019(COVID-19) Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)

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Antibacterial surfaces:Strategies and applications

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Science China(Technological Sciences) 2022年第5期65卷 1000-1010页

作者： YANG XiaoMeng HOU JianWen TIAN Yuan zhao jingya SUN QiangQiang ZHOU ShaoBing Key Laboratory of Advanced Technologies of Materials Ministry of EducationSchool of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu610031China School of Life Science and Engineering Southwest Jiaotong UniversityChengdu610031China

Antibacterial surfaces are surfaces that can resist bacteria,relying on the nature of the material itself.It is significant for safe food and water,human health,and industrial equipment.Biofilm is the main form of bacterial contamination on the material surface.Preventing the formation of biofilm is an efficient way to develop antibacterial surfaces.The strategy for constructing the antibacterial surface is divided into bacteria repelling and bacteria killing based on the formation of the biofilm.Material surface wettability,adhesion,and steric hindrance determine bacteria repelling performance.Bacteria should be killed by surface chemistry or physical structures when they are attached to a material surface irreversibly.Killing approaches are usually in the light of the cell membrane of bacteria.This review summarizes the fabrication methods and applications of antibacterial surfaces from the view of the treatment of the material surfaces.We also present several crucial points for developing long-term stability,no drug resistance,broad-spectrum,and even programable antibacterial surfaces.

关键词： antibacterial surface bacteria repelling bacteria killing

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乳腺癌患者组织中miR-150-5p表达及临床意义

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河南医学研究 2024年第5期33卷 872-875页

作者：李静亚赵艳杨柳荫河南省洛阳市妇幼保健院病理科河南洛阳471000 河南省洛阳市妇幼保健院外科河南洛阳471000

目的探讨乳腺癌患者组织中微小RNA-150-5p(miR-150-5p)表达及临床意义。方法前瞻性选取河南省洛阳市妇幼保健院2019年12月至2022年5月收治86例乳腺癌患者为对象,收集患者年龄、乳腺癌家族遗传史、肿瘤直径大小、疾病类型等一般资料,并... 详细信息

目的探讨乳腺癌患者组织中微小RNA-150-5p(miR-150-5p)表达及临床意义。方法前瞻性选取河南省洛阳市妇幼保健院2019年12月至2022年5月收治86例乳腺癌患者为对象,收集患者年龄、乳腺癌家族遗传史、肿瘤直径大小、疾病类型等一般资料,并测量所有患者癌组织与癌旁正常组织miR-150-5p水平。结果乳腺癌患者癌组织miR-150-5p水平高于癌旁正常组织(P<0.001)。miR-150-5p高水平组肿瘤直径大小、Ⅲ~Ⅳ期占比高于低水平组(P<0.05)。86例乳腺癌患者中共发生复发转移11例(12.79%),其中miR-150-5p低水平组3例,高水平组8例。logistic回归分析结果显示,miR-150-5p水平与乳腺癌复发转移有关(P<0.05)。结论乳腺癌患者癌组织中miR-150-5p呈异常高表达,可能是患者预后不良的指标之一。miR-150-5p水平与肿瘤直径、分期有关。

关键词：乳腺癌 miR-150-5p 复发转移肿瘤直径

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High-efficiency Agrobacterium-mediated transformation of chrysanthemum via vacuum infiltration of internode

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Ornamental Plant Research 2022年第1期2卷 1-7页

作者： Sujuan Xu Ze Wu jingya zhao Fengjiao Zhang Xinqi Zhang Fadi Chen Nianjun Teng Key Laboratory of Landscaping Agriculture Ministry of Agriculture and Rural Affairs/Key Laboratory of Biology of Ornamental Plants in East ChinaNational Forestry and Grassland AdministrationCollege of HorticultureNanjing Agricultural UniversityNanjing 210095China Nanjing Agricultural University-Nanjing Oriole Island Modern Agricultural Development Co. Ltd.Jiangsu Graduate WorkstationNanjing 210043China

Leaf disc transformation is one of the traditional methods that are now widely used in chrysanthemum with highly economical and ornamental value in world flower production,but it depends on plant genotypes and is time consuming and complicated.In addition,the transformation success rate of this method is low,generally ranging from 0.1%to 6.25%.Therefore,a highly efficient transformation system is needed.In this study,we are the first to establish a high-efficient chrysanthemum Agrobacterium-mediated transformation system via vacuum infiltration.Chrysanthemum stem internode explants were used as research material and CmLEC1 was used as a reporter gene.After approximately 3 months of culture and selection,the positive transgenic plants were obtained.Additionally,the positive probability was about 42%.The transformation efficiency was up to 37.7%,and if the escapes were removed,it was 16%.Furthermore,stable expression of CmLEC1 in transgenic'Yuhualuoying'was confirmed by qRT-PCR analysis.These results suggest that this genetic transformation system via vacuum infiltration of chrysanthemum stem internode is highly efficient and convenient,and much better than traditional leaf disc transformation,and it will play an important role in chrysanthemum transformation and functional genetics research.

关键词： transformation vacuum removed

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