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关于负压隔离病房可以使用循环风的讨论——国标《医院洁净护理与隔离单元技术标准》编制组系列探讨问题之二

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暖通空调 2019年第8期49卷 45-48页

作者：许钟麟曹国庆张益昭潘红红沈晋明刘燕敏谢景欣中国建筑科学研究院有限公司同济大学江苏省疾病预防控制中心

认为用全新风不是负压隔离病房的必要条件,一些国外标准也指明有高效过滤器时,可以使用回风。排风采用国产C类高效过滤器且保证边框无泄漏时,滤菌效率达99.999997%。病人一个喷嚏最多产生30万个/min菌粒,对于12h^-1换气的病房,1h打5个喷... 详细信息

认为用全新风不是负压隔离病房的必要条件,一些国外标准也指明有高效过滤器时,可以使用回风。排风采用国产C类高效过滤器且保证边框无泄漏时,滤菌效率达99.999997%。病人一个喷嚏最多产生30万个/min菌粒,对于12h^-1换气的病房,1h打5个喷嚏,采用循环风时平均有0.34×10^-4个/L微粒送入室内,和病人所在氛围中菌浓相比可视为0,故可使用循环风。从国外几个标准看,只要通过高效过滤器,允许使用室内自循环风。

关键词：负压隔离病房高效过滤器循环风全新风全排风

Hierarchical ferric-cobalt-nickel ternary oxide nanowire arrays supported on graphene fibers as high-performance electrodes for flexible asymmetric supercapacitors

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Nano Research 2018年第4期11卷 1775-1786页

作者： jingxin Zhao Chaowei Li Qichong Zhang Jun Zhang Xiaona Wang Juan Sun Juanjuan Wang Jixun xie Ziyin Lin Zhuo Li Weibang Lu Conghua Lu Yagang Yao School of Materials Science and Engineering Tianjin University Tianjin 300072 China Division of Advanced Nanomaterials Key Laboratory of Nanodevices and Applications Suzhou Institute of Nano-tech and Nano-bionics Chinese Academy of Sciences University of Chinese Academy of Sciences Suzhou 215123 China School of Materials Science and Engineering Georgia Institute of Technology 771 Ferst Drive Atlanta 30332 USA

Fiber-based supercapacitors （FSCs） are new members of the energy storage family. They present excellent flexibility and have promising applications in lightweight, flexible, and wearable devices. One of the existing challenges of FSCs is enhancing their energy density while retaining the flexibility. We developed a facile and cost-effective method to fabricate a highly capacitive positive electrode based on hierarchical ferric-cobalt-nickel ternary oxide nanowire arrays/graphene fibers and a negative electrode based on polyaniline-derived carbon nanorods/graphene fibers. The elegant microstructures and excellent electrochemical performances of both electrodes enabled us to construct a high- performance flexible asymmetric graphene fiber-based supercapacitor device with an operating voltage of 1.4 V, a specific capacitance up to 61.58 ***-2, and an energy density reaching 16.76 μW·h·cm-2. Moreover, the optimal device presents an outstanding cycling stability with 87.5% initial capacitance retention after 8,000 cycles, and an excellent flexibility with a capacitance retention of 90.9% after 4,000 cycles of repetitive bending.

关键词： fiber-based supercapacitors ferric-cobalt-nickel ternary oxide nanowire arrays polyaniline-derived carbon nanorods flexibility

制药工艺人工投料工序高危药物粉尘控制与净化装置研制

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暖通空调 2019年第6期49卷 17-21页

作者：冯灵云樊晶光张岩松师晓栋王建锋谢景欣夏信群赵振波国家卫生健康委员会职业安全卫生研究中心江苏省疾病预防控制中心 210009 浙江泰林生物技术股份有限公司

现阶段,制药工艺中的人工称量、投料和在线清洗工序存在开放式作业,致使作业人员暴露于存在药物粉尘的作业环境中。针对上述工序进行工艺改进,采用严格密闭、负压控制、通风净化、自动控制等措施,研发了一种适用于控制高危药物粉尘扩散... 详细信息

现阶段,制药工艺中的人工称量、投料和在线清洗工序存在开放式作业,致使作业人员暴露于存在药物粉尘的作业环境中。针对上述工序进行工艺改进,采用严格密闭、负压控制、通风净化、自动控制等措施,研发了一种适用于控制高危药物粉尘扩散,并净化送入和排出的空气,以及收集药物粉尘、具备药品生产与粉尘控制功能的一体化集成装置,既能满足生产工艺要求,又能控制药物粉尘危害、保障工人健康。

关键词：人工称量人工投料药物粉尘通风净化集成装置

A new generation M^(pro)inhibitor with potent activity against SARS-CoV-2 Omicron variants

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Signal Transduction and Targeted Therapy 2023年第4期8卷 1970-1982页

作者： Chong Huang Huiping Shuai jingxin Qiao Yuxin Hou Rui Zeng Anjie Xia Lingwan xie Zhen Fang Yueyue Li Chaemin Yoon Qiao Huang Bingjie Hu Jing You Baoxue Quan Xiu Zhao Nihong Guo Shiyu Zhang Ronggang Ma Jiahao Zhang Yifei Wang Ruicheng Yang Shanshan Zhang Jinshan Nan Haixing Xu Falu Wang Jian Lei Hin Chu Shengyong Yang State Key Laboratory of Biotherapy and Cancer Center and National Clinical Research Center for Geriatrics West China HospitalSichuan UniversityChengduSichuan 610041China State Key Laboratory of Emerging Infectious Diseases Li Ka Shing Faculty of MedicineThe University of Hong KongPokfulamHong Kong Special Administrative RegionChina Department of Microbiology Li Ka Shing Faculty of MedicineThe University of Hong KongPokfulamHong Kong Special Administrative RegionChina Key Laboratory of Drug Targeting and Drug Delivery Systems Ministry of EducationWest China School of PharmacySichuan UniversityChengduSichuan 610041China

Emerging SARS-CoV-2 variants, particularly the Omicron variant and its sublineages, continually threaten the global public *** molecule antivirals are an effective treatment strategy to fight against the virus. However, the first-generation antivirals eithershow limited clinical efficacy and/or have some defects in pharmacokinetic (PK) properties. Moreover, with increased use of thesedrugs across the globe, they face great pressure of drug resistance. We herein present the discovery and characterization of a newgeneration antiviral drug candidate (SY110), which is a potent and selective inhibitor of SARS-CoV-2 main protease (Mpro). Thiscompound displayed potent in vitro antiviral activity against not only the predominant SARS-CoV-2 Omicron sublineage BA.5, butalso other highly pathogenic human coronaviruses including SARS-CoV-1 and MERS-CoV. In the Omicron-infected K18-hACE2mouse model, oral treatment with SY110 significantly lowered the viral burdens in lung and alleviated the virus-induced ***, SY110 possesses favorable PK properties with high oral drug exposure and oral bioavailability, and also an outstandingsafety profile. Furthermore, SY110 exhibited sensitivity to several drug-resistance Mpro mutations. Collectively, this investigationprovides a promising new drug candidate against Omicron and other variants of SARS-CoV-2.

关键词： treatment predominant potent

光催化对洁净厂房循环风单元中甲醛净化参数的优化研究

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暖通空调 2019年第10期49卷 92-96页

作者：徐凯丽伏相瑾夏宇阳韩昶玉钟丽诗范钰莹谢景欣王建锋沈卫民凌晨东南大学江苏省疾病预防控制中心 210009 苏州市金燕净化设备工程有限公司

为了探讨洁净厂房循环风单元回风净化装置中光催化剂种类、用量、光照强度等参数对降解甲醛效率的影响,以确定光催化净化网参数配置的方案。采用酚试剂分光光度法测定甲醛浓度,在60 m^3的模拟洁净厂房内,通过正交试验筛选回风净化装置... 详细信息

为了探讨洁净厂房循环风单元回风净化装置中光催化剂种类、用量、光照强度等参数对降解甲醛效率的影响,以确定光催化净化网参数配置的方案。采用酚试剂分光光度法测定甲醛浓度,在60 m^3的模拟洁净厂房内,通过正交试验筛选回风净化装置中光催化净化网对甲醛15 min降解效率的最优参数组合。试验结果表明,光催化剂用量对甲醛降解效率有显著性差异。直接分析发现净化参数对甲醛降解效率的影响大小顺序为光催化剂用量>光照强度>光催化剂种类。综合分析得出光催化净化网最佳参数配置为纳米TiO2、光催化剂用量三遍(30 g/m^2)、紫外灯20 W。光催化净化网的最佳参数配置能够提高光催化净化网对洁净厂房中甲醛的降解效率。

关键词：光催化纳米TiO2 纳米WO3 光照强度降解效率

Shortened snRNA promoters for efficient CRISPR/Cas-based multiplex genome editing in monocot plants

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Science China(Life Sciences) 2020年第6期63卷 933-935页

作者： Yu Hao Wubei Zong Dongchang Zeng Jingluan Han Shuifu Chen Jianian Tang Zhe Zhao Xiaojuan Li Kun Ma Xianrong xie Qinlong Zhu Yuanling Chen Xiucai Zhao jingxin Guo Yao-Guang Liu State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangzhou 510642.China College of Life Sciences South China Agricultural UniversityGuangzhou 510642.China Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510642.China

Dear Editor,CRISPR(clustered regularly interspaced short palindromic repeats)/Cas genome editing is a powerful tool for introducing specific mutations in organisms including *** system is composed of a nuclease such as Cas9 or Cas12a and an engineered single-guide RNA(sgRNA)incorporating a target sequence(Li et al.,2019).A Cas9/sgRNA complex recognizes its target site in the genome,resulting in a mutation at that site.

关键词： RNA CRISPR Shortened snRNA promoters for efficient CRISPR/Cas-based multiplex genome editing in monocot plants

Rice OsUBR7 modulates plant height by regulating histone H2B monoubiquitination and cell proliferation

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Plant Communications 2022年第6期3卷 251-266页

作者： Yangyi Zheng Sensen Zhang Yanqiu Luo Fuquan Li Jiantao Tan Bin Wang Zhe Zhao Huifang Lin Tingting Zhang Jianhong Liu Xupeng Liu jingxin Guo Xianrong xie Letian Chen Yao-Guang Liu Zhizhan Chu State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources College of Life SciencesSouth China Agricultural UniversityGuangzhou 510642China Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510642China Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms South China Agricultural UniversityGuangzhou 510642China

Plant height is an important agronomic trait for lodging resistance and ***,we report a new plantheight-related gene,OsUBR7 in rice(Oryza sativa L.);knockout of OsUBR7 caused fewer cells in internodes,resulting in a semi-dwarf ***7 encodes a putative E3 ligase containing a plant homeodomain finger and a ubiquitin protein ligase E3 component N-recognin 7(UBR7)***7 interacts with histones and monoubiquitinates H2B(H2Bub1)at lysine148 in coordination with the E2 conjugase ***7 mediates H2Bub1 at a number of chromatin loci for the normal expression of target genes,including cell-cycle-related and pleiotropic genes,consistent with the observation that cell-cycle progression was suppressed in the osubr7 mutant owing to reductions in H2Bub1 and expression levels at these *** genetic divergence of OsUBR7 alleles among japonica and indica cultivars affects their transcriptional activity,and these alleles may have undergone selection during rice ***,our results reveal a novel mechanism that mediates H2Bub1 in plants,and UBR7 orthologs could be utilized as an untapped epigenetic resource for crop improvement.

关键词： histone H2B monoubiquitination E3 ligase UBR7 plant height rice

小头畸形-癫痫-发育迟缓综合征1家系遗传学分析并文献复习

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中华围产医学杂志 2022年第10期25卷 788-792页

作者：徐琪琪谢建生吴维青李博红刘洋杨京鑫南方医科大学第一临床医学院(深圳市妇幼保健院)医学遗传中心深圳518084

目的探讨小头畸形-癫痫-发育迟缓(microcephaly-seizures-development delay,MCSZ)综合征的临床表现和遗传学病因。方法对2018年6月在本院就诊的1个MCSZ家系中患儿及其父母行全基因外显子组测序和生物信息学分析,将所得的致病性变异用Sa... 详细信息

目的探讨小头畸形-癫痫-发育迟缓(microcephaly-seizures-development delay,MCSZ)综合征的临床表现和遗传学病因。方法对2018年6月在本院就诊的1个MCSZ家系中患儿及其父母行全基因外显子组测序和生物信息学分析,将所得的致病性变异用Sanger测序法验证。明确患儿的分子诊断后,对该家系2020年再妊娠胎儿行产前基因诊断。并通过文献复习,总结和分析国内外已报道的MCSZ综合征病例的临床特征及遗传学特点。结果(1)本病例:患儿女性,8月龄,因“头围小,反复抽搐”收入院,临床表现为小头畸形、多发性癫痫、精神发育迟缓。听觉诱发电位示左侧耳神经通路中度损害,全外显子组测序发现患儿存在PNKP基因复合杂合变异。其中致病性c.199-10_203delinsTCTGAGGGGT变异遗传自父亲,可能致病性c.1505C>T(p.P502>L)变异遗传自母亲,两变异均为文献未报道的变异。结合临床表现考虑遗传学病因为PNKP基因复合杂合变异。该家系再孕胎儿未遗传来自父母的PNKP基因变异,夫妇选择继续妊娠。随访至2021年12月,再孕胎儿为女性,现13月龄,枕额围测值、语言、运动发育正常。(2)文献复习:共检索到相关文献12篇、MCSZ综合征患者38例,合并本例共39例。39例患儿的临床表型包括:小头畸形(91.7%,33/36)、癫痫(88.2%,30/34)、发育迟缓(96.4%,27/28)、多动症(25.6%,10/39)、胃食管反流(10.3%,4/39)和听力丧失(7.7%,3/39)。其中癫痫首发年龄集中在婴幼儿期(96.3%,26/27),头颅MRI检查异常比例为91.2%(31/34)。结论对于产前发现胎儿头围偏小,且进行性缩小表现,出生后伴有小头畸形、癫痫、发育迟缓、多动症、胃食管反流和听力丧失等临床特征的患者,应当考虑MCSZ综合征可能。此类患儿临床预后差异大,基因检测有助于早期诊断和遗传咨询。

关键词：小头畸形癫痫发育障碍听力损失磷酸转移酶类(醇族体) DNA修复酶类全外显子组测序产前诊断

A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants

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Molecular Plant 2015年第8期8卷 1274-1284页

作者： Xingliang Ma Qunyu Zhang Qinlong Zhu Wei Liu Yan Chen Rong Qiu Bin Wang Zhongfang Yang Heying Li Yuru Lin Yongyao xie Rongxin Shen Shuifu Chen Zhi Wang Yuanling Chen jingxin Guo Letian Chen Xiucai Zhao Zhicheng Dong Yao-Guang Liu State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangzhou 510642 China Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions Guangzhou 510642 China Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms Guangzhou 510642 China College of Life Sciences South China Agricultural University Guangzhou 510642 China Key Laboratory of South China Agriculture Plant Molecular Analysis and Genetic Improvement South China Botanical Garden Chinese Academy of SciencesGuangzhou 510650 China

CRISPR/Cas9 genome targeting systems have been applied to a variety of species. However, most CRISPR/Cas9 systems reported for plants can only modify one or a few target sites. Here, we report a robust CRISPR/Cas9 vector system, utilizing a plant codon optimized Cas9 gene, for convenient and high- efficiency multiplex genome editing in monocot and dicot plants. We designed PCR-based procedures to rapidly generate multiple sgRNA expression cassettes, which can be assembled into the binary CRISPR/ Cas9 vectors in one round of cloning by Golden Gate ligation or Gibson Assembly. With this system, we edi- ted 46 target sites in rice with an average 85.4% rate of mutation, mostly in biallelic and homozygous status. We reasoned that about 16% of the homozygous mutations in rice were generated through the non-homol- ogous end-joining mechanism followed by homologous recombination-based repair. We also obtained uni- form biallelic, heterozygous, homozygous, and chimeric mutations in Arabidopsis T1 plants. The targeted mutations in both rice and Arabidopsis were heritable. We provide examples of loss-of-function gene mu- tations in To rice and T1Arabidopsis plants by simultaneous targeting of multiple （up to eight） members of a gene family, multiple genes in a biosynthetic pathway, or multiple sites in a single gene. This system has provided a versatile toolbox for studying functions of multiple genes and gene families in plants for basic research and genetic improvement.

关键词： sequence-specific nucleases genome editing CRISPR/Cas9 rice Arabidopsis