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Chinese Journal of Chemistry 2014年第4期32卷 293-297页

作者： Hanzhi Zhang Chao Liu Xuepei Zhang jingwu kang Shanghai Institute of Organic Chemistry Chinese Academy of SciencesShanghai 200032China

A simple approach for the preparation of emitter of electrospray ionization mass spectrometry by covalently coating the fused-silica capillary tip with the conductive carbon-nanotube sol-gel composite material(CNTSCM)is described.The CNTSCM was prepared by dispersing single-walled carbon nanotubes in the sol composed of a mixture of 3-glycidoxypropyltrimethoxysilane and 3-aminopropyltrimethoxysilane,and ethanol.The long-term sta-bility of the prepared ESI emitters was demonstrated by at least 180 h of continuous use.Signal intensity obtained by the prepared emitter was mass-flux sensitive when the flow rate was lower than 500 nL/min,while the signal in-tensity performed a concentration dependence when the flow rate was in the range of 500-800 nL/min.The use-fulness of such a prepared emitter was demonstrated by the analysis of various types of samples such as organic small molecular drugs,oligosaccharide,peptide,and protein.

关键词： electrospray emitter mass spectrometry carbon-nanotube sol-gel composite material

Drug Target Identification Using Affinity Core-Shell Magnetic Nanoparticles and Mass Spectrometry

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Chinese Journal of Chemistry 2013年第6期31卷 715-720页

作者： Yunhuan Wei Tongdan Wang Chao Liu Qianqian Zhang Lishun Wang Gongli Tang jingwu kang Shanghai Institute of Organic Chemistry Chinese Academy of Sciences Shanghai 200032 China Key Laboratory of Cell Differentiation andApoptosis of Chinese Ministry of Education Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200025 China National Key Laboratory of Organic Biochemistry Opening Foundations Shanghai 200032 China

亲密关系核心壳当与基于的 nano 高效的液体层析双人脚踏车团 spectrometry (HPLC-MS/MS ) 在联合使用了时，磁性的 nanoparticles (MNP ) 为在房间 lysate 识别药的目标蛋白质作好准备猎枪 proteomic 分析。cyclosporine A (CsA ) 的... 详细信息

亲密关系核心壳当与基于的 nano 高效的液体层析双人脚踏车团 spectrometry (HPLC-MS/MS ) 在联合使用了时，磁性的 nanoparticles (MNP ) 为在房间 lysate 识别药的目标蛋白质作好准备猎枪 proteomic 分析。cyclosporine A (CsA ) 的很多个新潜在的目标能被识别，由于在药目标鉴定的亲密关系 MNP 的高功效。就我们的知识而言，这是第一次揭示 CsA 的如此的一个丰富的目标系列。

关键词：磁性纳米粒子串联质谱法药物靶点亲和力核壳型细胞裂解液液相色谱蛋白质组

Profiling Drug-Protein Interactions by Micro Column Affinity Purification Combined with Label Free Quantification Proteomics

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Chinese Journal of Chemistry 2020年第12期38卷 1681-1685页

作者： Li Gong Yao Xu Guizhen Liu Mengmeng Zheng Xuepei Zhang Ying Hang jingwu kang State Key Laboratory of Bioorganic and Natural Products Chemistry Center for Excellence in Molecular SynthesisShanghai Institute of Organic ChemistryChinese Academy of Sciences345 Lingling RoadShanghai 200032China School of Physical Science and Technology ShanghoiTech UniversityHaike Rood 100Shanghai 200120China School of Life Science and Technology ShanghaiTech UniversityHaike Road 100Shanghai 200120China

of main observation and conclusion We developed a method for comprehensively profiling drug-protein interactions using micro-column affinity purification(AP)combined with label-free quantitative(LFQ)proteomics as well as the statistical and bioinformatics analysis.FK506 was used as the experimental model for proof of concept.The true interacting proteins were distinguished from the background proteins by their fold changes of FLQ intensities combined with p-values.Totally 116 FK506 interacting proteins including 5 known target proteins were identified.The method was validated by using the LFQ intensity of the endogenous known drug targets together with statistical analysis.We demonstrated that the micro-column-based affinity purification in combination with LFQ proteomics provides a highly reproducible and robust approach for profiling drug-protein interactions.

关键词： proof analysis. conclusion

Ultra High Sensitive Determine The Optical Impurities of Sitafloxacin by Capillary Electrophoresis with Cyclodextrin and Ligand-Exchange as Chiral Additives

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Ultra High Sensitive Determine The Optical Impurities of Sit...

第21届全国色谱学术报告会及仪器展览会

作者： Ranran Meng jingwu kang Shanghai Institute of Organic Chemistry Chinese Academy of Sciences

＜正＞In this paper, a robust chiral separation method for the separation of four enantiomers of sitafloxacin was developed. Method was developed with two kinds of chiral selectors including cyclodextrin and Ligand-exchange as chiral additives. The method was optimized with the aid of response surface methodology （RSM） and Derringer’s desirability function. A central composite design was utilized to

关键词： enantioseparation capillary electrophoresis stereoisomeric impurity experimental design

Screening of Jumonji domain-containing protein 3 inhibitors innatural products by capillary electrophoresis

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Screening of Jumonji domain-containing protein 3 inhibitors ...

中国化学会第22届全国色谱学术报告会及仪器展览会

作者： Yi Zhang jingwu kang State Key Laboratory of Bio-organic and Natural Products Chemistry Center for Excellence in Molecular Synthesis Shanghai Institute of OrganicChemistry University of Chinese Academy of Sciences Chinese Academy of Sciences

＜正＞Jumonji domain-containing protein 3（JMJD3/KDM6 B） plays an important role intheepigeneticregulation of gene expression.Its abnormal expressioncan be observed in the progression ofa variety of diseases, such as... 详细信息

关键词： KDM6B inhibitor natural products capillary electrophoresis

Screening of Histone Deacetylase 1 Inhibitors in Natural Products by Capillary Electrophoresis

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Screening of Histone Deacetylase 1 Inhibitors in Natural Pro...

中国化学会第22届全国色谱学术报告会及仪器展览会

作者： Yanmei Zhang Feng Li jingwu kang State Key Laboratory of Bioorganic and Natural Products Chemistry Shanghai Institute of Organic Chemistry Chinese Academy of Sciences Institute for Agri-food Standards and Testing Technology Shanghai Academy of Agricultural Sciences

＜正＞A method for screening of histone deacetylase（HDAC） inhibitors in natural products by using capillary electrophoresis（CE） equipped with laser induced fluorescence（LIF） detection was developed. The method was developed by employing 5-carboxyfluorescein labelled peptide with an acetylated lysine residue as the substrate of HDAC1 and a small chemical library composed of 38 purified natural products. The biochemical assay was performed by the means of CE separation,

关键词： histone deacetylase inhibitor natural products capillary electrophoresis

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毛细管电泳-无鞘流电喷雾质谱用于药物分析

色谱 2023年第2期41卷 160-167页

作者：张含智李凤康经武生命有机化学国家重点实验室中国科学院上海有机化学研究所上海200032 西安市食品安全检测与风险评估重点实验室西安文理学院化学工程学院陕西西安710065 弈柯莱生物科技(上海)股份有限公司上海200241

毛细管电泳-质谱联用技术具有分离效率高、检测灵敏度高、样品消耗量少,可同时提供样品的结构信息等优点,成为复杂样品分离分析的强有力工具。但是,毛细管电泳与质谱联用的接口技术依然未能很好的解决。为了拓展我们发展的金箔包裹的毛... 详细信息

毛细管电泳-质谱联用技术具有分离效率高、检测灵敏度高、样品消耗量少,可同时提供样品的结构信息等优点,成为复杂样品分离分析的强有力工具。但是,毛细管电泳与质谱联用的接口技术依然未能很好的解决。为了拓展我们发展的金箔包裹的毛细管电泳分离柱尖端直接作为喷雾电极和无鞘流质谱接口的应用,本文报道了用无鞘流接口毛细管电泳-电喷雾质谱联用(CE-ESI-MS)分析5种酪氨酸激酶抑制剂(舒尼替尼、甲磺酸伊马替尼、吉非替尼、达沙替尼、埃罗替尼)的研究结果。这种接口集分离与电喷雾离子化于一根毛细管中,制作简单,成本低廉,且可批量制作。实验发现采用非水毛细管电泳分离模式不仅可以对5种酪氨酸激酶抑制剂实现基线分离,而且可以获得稳定的质谱信号。考察了电解质溶液组成对分离效果的影响,得到优化的背景电解质组成,即含2%(v/v)乙酸及5 mmol/L乙酸铵的乙腈-甲醇(80∶20,v/v)混合溶剂。在优化的条件下,5种激酶抑制剂可以得到基线分离,无鞘接口也可以长时间保持稳定的电喷雾,分析物的保留时间日内、日间重复性(RSD值)分别小于0.5%和0.8%,接口批次间的RSD值小于2.6%。与水相分离条件下的CE-MS对比,非水相条件下的5种酪氨酸激酶抑制剂的分离柱效更高,检测灵敏度更高,绝对检出限达到amol级。此外,采用无鞘流CE-MS分析了各类有机酸(千层纸素A、丹酚酸C和迷迭香酸)和脂溶性的大环内酯类抗生素(阿奇霉素、红霉素和环孢素A),均可以获得良好的分离效果和质谱检测结果。

关键词：无鞘流接口非水毛细管电泳-质谱金箔包裹的电喷雾电极酪氨酸激酶抑制剂

Screening of Small-Molecule Inhibitors of Protein-Protein Interaction with Capillary Electrophoresis Frontal Analysis

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Screening of Small-Molecule Inhibitors of Protein-Protein In...

第21届全国色谱学术报告会及仪器展览会

作者： Mei Xu Chao Liu Mi Zhou Qing Li Renxiao Wang jingwu kang State Key Laboratory of Bioorganic and Natural Products Chemistry Shanghai Institute of Organic ChemistryChinese Academy of Sciences ShanghaiTech University

A simple and effective method for identifying inhibitors of protein-protein interactions（PPIs） was developed by using capillary electrophoresis frontal analysis（CE-FA）. Anti-apoptotic B-cell-2（Bcl-2）family member Bcl-XL protein, a 5-carboxyfluorescein labelled peptide truncated from the BH3 domain of Bid（F-Bid） as the ligand, and a known Bcl-XL-Bid interaction inhibitor ABT-263 were employed as experimental model for the proof of concept. In CE-FA, the free ligand is separated from the protein and protein-ligand complex to permit the measurement of the equilibrium concentration of the ligand, hence the dissociation constant of the protein-ligand complex. In the presence of inhibitors, formation of the protein-ligand complex is hindered, thereby the inhibition can be easily identified by the raised plateau height of the ligand and the decayed plateau of the complex. Further, we proposed an equation used to convert the IC50 value into the inhibition constant Ki value, which is more useful than the former for comparison. In addition, the sample pooling strategy was employed to improve the screening throughput more than ten times. A small chemical library composing of synthetic compounds and natural extracts were screened with the method, two natural products, namely demethylzeylasteral and celastrol were identified as new inhibitors to block the Bcl-XL-Bid interaction. Cell-based assay was performed to validate the activity of the identified compounds. The result demonstrated that CE-FA represents a straightforward and robust technique for screening of PPI inhibitors.

关键词： Protein-protein interactions Anti-apoptotic B-cell-2（Bcl-2）￡?Drug screening Capillary electrophoresis frontal analysis

Recent advances in the application of MXenes for neural tissue engineering and regeneration

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Neural Regeneration Research 2024年第2期19卷 258-263页

作者： Menghui Liao Qingyue Cui Yangnan Hu Jiayue Xing Danqi Wu Shasha Zheng Yu Zhao Yafeng Yu jingwu Sun Renjie Chai State Key Laboratory of Digital Medical Engineering Department of Otolaryngology Head and Neck SurgeryZhongda HospitalSchool of Life Sciences and TechnologyAdvanced Institute for Life and HealthJiangsu Province High-Tech Key Laboratory for Bio-Medical ResearchSoutheast UniversityNanjingJiangsu ProvinceChina Co-Innovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Oto-Rhino-Laryngology West China HospitalSichuan UniversityChengduSichuan ProvinceChina First Affiliated Hospital of Soochow University SuzhouJiangsu ProvinceChina Department of Otolaryngology-Head and Neck Surgery The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Department of Otolaryngology Head and Neck Surgery Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan ProvinceChina Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijingChina Beijing Key Laboratory of Neural Regeneration and Repair Capital Medical UniversityBeijingChina

Transition metal carbides and nitrides(MXenes)are crystal nanomaterials with a number of surface functional groups such as fluorine,hydroxyl,and oxygen,which can be used as carriers for proteins and drugs.MXenes have excellent biocompatibility,electrical conductivity,surface hydrophilicity,mechanical properties and easy surface modification.However,at present,the stability of most MXenes needs to be improved,and more synthesis methods need to be explored.MXenes are good substrates for nerve cell regeneration and nerve reconstruction,which have broad application prospects in the repair of nervous system injury.Regarding the application of MXenes in neuroscience,mainly at the cellular level,the long-term in vivo biosafety and effects also need to be further explored.This review focuses on the progress of using MXenes in nerve regeneration over the last few years;discussing preparation of MXenes and their biocompatibility with different cells as well as the regulation by MXenes of nerve cell regeneration in two-dimensional and three-dimensional environments in vitro.MXenes have great potential in regulating the proliferation,differentiation,and maturation of nerve cells and in promoting regeneration and recovery after nerve injury.In addition,this review also presents the main challenges during optimization processes,such as the preparation of stable MXenes and long-term in vivo biosafety,and further discusses future directions in neural tissue engineering.

关键词： hydrogels MXenes nerve regeneration neural cells neural stem cells organoids spiral ganglion neurons

伴刀豆凝集素修饰磁性纳米粒子富集人血清中糖蛋白及质谱鉴定

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色谱 2014年第4期32卷 369-375页

作者：李凤康经武中国科学院上海有机化学研究所上海200032

发展了一种新型的磁性纳米粒子应用于人血清中特异性糖蛋白的亲和富集。制备的磁性纳米粒子具有核/壳/壳结构,即由Fe3O4磁性粒子/硅胶层/有机聚合物外层构成。伴刀豆凝集素A(Con A)以共价键合的形式通过短链聚乙二醇固定在粒子表面,实... 详细信息

发展了一种新型的磁性纳米粒子应用于人血清中特异性糖蛋白的亲和富集。制备的磁性纳米粒子具有核/壳/壳结构,即由Fe3O4磁性粒子/硅胶层/有机聚合物外层构成。伴刀豆凝集素A(Con A)以共价键合的形式通过短链聚乙二醇固定在粒子表面,实现了人血清中特异性糖蛋白的高效富集。富集的蛋白经过胰蛋白酶酶解后,所得的肽段经离线的二维色谱分离,用高分辨质谱共鉴定出80种蛋白。通过NetNGlyc等搜索软件分析确定其中76种为糖蛋白,分析发现在血清中质量浓度仅为0.000 01 g/L的β-2-glycoprotein 1也得到了鉴定,表明我们发展的磁性纳米粒子与凝集素相结合的方式,可以高效地富集复杂体系中与主要蛋白成分含量相差12个数量级的低丰度糖蛋白。

关键词：液相色谱质谱富集糖蛋白凝集素磁性纳米粒子