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Development of a soft X-ray microprobe for single cell radiobiology

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Nuclear Science and Techniques 2009年第2期20卷 82-86页

作者： CHEN Liang YAN jingwen JIANG Shiping yu Yang National Synchrotron Radiation Laboratory University of Science ＆ Technology of China Hefei 230029 China

An X-ray microprobe for radiobiological studies was developed which deliver precise doses of radiation to the selected individual *** facility used synchrotron radiation as soft X-ray source.A zone plate combining with a pinhole produced a fine probe from bending magnet for single cell irradiating with defined *** diameter of microprobe at the target position was about 2 μm by scanning a knife-edge with an AXUV photo *** fluxes of soft X-rays at 516.7 eV(2.4 nm) were about 5.4×104 photons/s.100mA measured with the photo *** absorbed dose rate for typical yeast cells was about 11.34 Gy/s with the storage current of 100 mA.A preliminary experiment for yeast cells irradiation has shown that the microprobe had a definite biological effect for radiobiological *** soft X-ray microprobe at "water window" region has provided a useful tool for single cell irradiating damage and a capability of individually irradiating a certain numbers of cells each time.

关键词： X射线微探针放射生物学单细胞软X射线源辐射生物学效应光电二极管照射剂量同步辐射

Rational design for fungal laccase production in the model host Aspergillus nidulans

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Science China(Life Sciences) 2019年第1期62卷 84-94页

作者： Wei Li jingwen yu Zixin Li Wen-Bing Yin State Key Laboratory of Mycology Institute of Microbiology Chinese Academy of Sciences Savaid Medical School University of Chinese Academy of Sciences

Laccases, multicopper oxidoreductases, are mainly produced in white-rot fungi and are considered as ideal green catalysts in industrial and biotechnological applications. However, the development of laccases is limited due to the slow growth of natural laccase producing strains and the low expression levels of laccases. In this study, we designed three regulation strategies for laccase gene expression in the model fungus Aspergillus nidulans. By introducing various promoters in front of the laccase gene pslcc from the white-rot fungus Pycnoporus sanguineus, we found that the laccase gene with the original promoter had effective expression in A. nidulans. Using the previously identified transcription factor RsmA regulatory mechanism, the aflR promoter was inserted into the pslcc expression vectors, and the laccase production was 15-fold higher in the strain overexpressing of RsmA compared to the control strain. To improve the laccase yield, the dipeptidyl-peptidase DppV, aspartic protease PepA and mannosyltransferase Mnn9 were successfully deleted in the A. nidulans host. The laccase activities were increased approximately 8-fold and 13-fold in the double deletions strains of Δmnn9ΔpepA and ΔdppVΔpepA over the control strains, *** together, these results not only demonstrate an efficient system for heterologous protein production in the model fungus *** but also provide a general approach to applying regulatory methods to control gene expression.

关键词： laccase heterologous expression promoter Aspergillus nidulans

Long-term trend of water vapor over the Tibetan Plateau in boreal summer under global warming

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Science China Earth Sciences 2022年第4期65卷 662-674页

作者： jingwen yu Qingquan LI Yihui DING Jie ZHANG Qingyuan WU Xinyong SHEN Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters/Key Laboratory of Meteorological Disaster Ministry of Education/Joint International Research Laboratory on Climate and Environment ChangeNanjing University of Information Science&TechnologyNanjing 210044China Laboratory for Climate Studies National Climate CenterChina Meteorological AdministrationBeijing 100081China Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) Zhuhai 519082China

In this study,the long-term trend of water vapor over the Tibetan Plateau(TP)in boreal summer is investigated by using observation and reanalysis data from 1979 to *** historical experiment simulations of 19 models that participated in the Coupled Model Intercomparison Project phase 6(CMIP6)are evaluated,and the future variation tendency under four emission scenarios is *** results indicate that the water vapor content and the net water vapor budget over the TP show notable increasing trends,which are mainly manifested by a significant increase in the net water vapor import and a significant decrease in the water vapor export on the eastern boundary of the *** is mainly due to an anomalous anticyclone from Lake Baikal to the Mongolian *** CMIP6 multi-model ensemble can well simulate the variation characteristics of the TP net water vapor *** projection results indicate that by the end of the twenty-first century,the water vapor content,the net water vapor import and precipitation over the TP will *** a high-emissions scenario and compared with the current period(1991–2014),these three variables will increase by 47.99%,59.77%and 18.59%in the long term(2081–2100),*** significant enhancement of meridional water vapor transport over the northern TP may be the main reason for the increase in humidity over the TP.

关键词： Tibetan Plateau Boreal summer Water vapor transport Long-term trend Projection

Pain or Gain?Chinese Experience of Capital Account Liberalization

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China & World Economy 2019年第5期27卷 79-107页

作者： Hongfeng Peng jingwen yu School of Finance Shandong University of Finance and EconomicsChina School of Economics and Management Wuhan UniversityChina

The neoclassical growth model predicts that capital account liberalization could potentially enhance economic performance;however, there is no consistent empirical evidence to support this positive association. Using a novel dataset of Chinese capital account openness, this paper demonstrates a positive relationship between capital account liberalization and aggregate economic performance. The difference-indifferences method is used to capture the causal effect of capital account liberalization on economic performance by taking advantage of variations in both external financial dependence and the progress of capital account openness. We investigate three channels that could strengthen this positive relationship using a firm-level dataset. We find that capital account liberalization could:(i) alleviate the degree of resource misallocation, and this effect is more significant in industries relying heavily on external finance and in regions with more favorable business environments;(ii) enhance firms' total factor productivity;and (iii) promote innovation. Our findings suggest that a strategy of gradual openness will leave some leeway not only for improvement in domestic markets but also to mitigate exposure to unfavorable global shocks.

关键词： capital account liberalization difference-in-differences innovation productivity

What Role Does the Political Environment Play in Economic Development？ --A Case Study of Fujian Province

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Frontiers of Economics in China-Selected Publications from Chinese Universities 2012年第4期7卷 544-559页

作者： jingwen yu Chunchao Wang School of Economics Fudan University Shanghai 200433 China Department of Economics Jinan University Guangzhou 510632 China

Uncertainty induced by the political environment affects investment risk, and thus affects investment decisions, which have a close relationship with economic development. This paper investigates the economic cost of political instability using the case study of the tense relationship across-Strait in China. We use a synthetic control method to better model the counterfactual analysis of this case study. The intense situation of the relations across-Strait has great influence on the economic development of Fujian province. Fujian province is the closest province in proximity to Taiwan and also possesses the greatest preferential policies for Taiwan Residents direct investment. The empirical results of this study reveal that during 2001-2008 Fujian province＇s average annual loss in GDP per capita was 682.54 yuan. In other words, GDP per capita in Fujian has declined about 12.1 percent annually during this period compared with GDP per capita as calculated by the synthetic control method.

关键词： political environment economic development synthetic controlmethod cross-straits relations

Waste activated sludge treatment based on temperature staged and biologically phased anaerobic digestion system

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Journal of Environmental Sciences 2013年第10期25卷 2056-2064页

作者： jingwen yu1 Mingxia Zheng Tao Tao Jiane Zuo Kaijun Wang School of Environmental Science and Engineering Huazhong University of Science and Technology State Key Joint Laboratory of Environment Simulation and Pollution Control School of EnvironmentTsinghua University

The concept of temperature staged and biological phased （TSBP） was proposed to enhance the performance of waste-activated sludge anaerobic digestion. Semi-continuous experiments were used to investigate the effect of temperature （35 to 70℃） as well as the hydraulic retention time （HRT）（2, 4 and 6 days） on the acidogenic phase. The results showed that the solubilization degree of waste- activated sludge increased from 14.7% to 30.1% with temperature increasing from 35 to 70℃, while the acidification degree was highest at 45℃ （17.6%）, and this was quite different from the temperature impact on hydrolysis. Compared with HRT of 2 and 6 days, 4 days was chosen as the appropriate HRT because of its relatively high solubilization degree （24.6%） and acidification degree （20.1%） at 45℃. The TSBP system combined the acidogenic reactor （45℃, 4 days） with the methanogenic reactor （35℃, 16 days） and the results showed 84.8% and 11.4% higher methane yield and volatile solid reduction, respectively, compared with that of the single-stage anaerobic digestion system with HRT of 20 days at 35℃. Moreover, different microbial morphologies were observed in the acidogenic- and methanogenic-phase reactors, which resulted from the temperature control and HRT adjustment. All the above results indicated that 45℃ was the optimum temperature to inhibit the activity of methanogenic bacteria in the acidogenic phase, and temperature staging and phase separation was thus accomplished. The advantages of the TSBP process were also confirmed by a full-scale waste-activated sludge anaerobic digestion project which was an energy self-sufficient system.

关键词： temperature-staged and biologically-phased acidogenic and methanogenic phases temperature hydraulic retention time

The phototherapy toward corneal neovascularization elimination:An efficient,selective and safe strategy

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Chinese Chemical Letters 2021年第1期32卷 99-101页

作者： jingwen yu Chengchao Chu Yiming Wu Gang Liu Wei Li Eye Institute of Xiamen University Fujian Provincial Key Laboratory of Ophthalmology and Visual ScienceSchool of MedicineXiamen UniversityXiamen 361102China State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics&Center for Molecular Imaging and Translational Medicine School of Public HealthXiamen UniversityXiamen 361102China

Corneal neovascularization(CNV) can induce severe visual impairment and even *** treatments have limited efficacy and some undesirable side ***(FT) is a modern medical technique in CNV treatment and worth further *** has various advantages and nanoparticle-mediated drug delive ry also contributes to CNV *** combined with functional nanoparticles featuring photoacoustic imaging contrast properties can accomplish accurate and safe inhibition of CNV,thus is promising in clinical application.

关键词： Corneal neovascularization Phototherapy Nanotechnology R-nano ICG Photoacoustic imaging

Functional Characterization of a Type 4 Metallothionein Gene(CsMT4) in Cucumber

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Horticultural Plant Journal 2019年第3期5卷 120-128页

作者： Ling Duan jingwen yu Li Xu Ping Tian Xin Hu Xiaomei Song yu Pan Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education College of Horticulture and Landscape Architecture Southwest University

The excess amounts of heavy metals are toxic to various physiological processes in *** metallothioneins(MTs)are low molecular weight,cysteine-rich metal-binding proteins that play important roles in the detoxification of heavy metal *** this study,we characterized Cs MT4,a plant type 4 MT gene identified in a complementary DNA(cDNA)library prepared from young cucumber(Cucumis sativus)*** MT4encodes a 90 amino acid protein with a predicted molecular mass of 9.028 ***4 contains 17 cysteine residues in three highly conserved cysteine-rich *** contrast to the structures of other MTs,the highly conserved amino acid pattern CxCxxxCxCxxCxC is present in the middle of ***,CsMT4 was markedly induced in various tissues by various concentrations of Cd^(2+)and Zn^(2+)(>1.0 mmol·L^(-1)).Similar to AtMT4b function,heterologous expression of Cs MT4 in *** could also improved its tolerance to Cd^(2+)and led to increased uptake of Cd^(2+),and the rate of Cd^(2+)uptake was the highest in cells expressing a phytochelatin-like *** findings demonstrate that CsMT4 might be obviously induced by the metal stress in cucumber,and improves tolerance to Cd ions but not Zn ions when heterologously expressed in ***,and suggest that the composition and arrangement of N-terminal Cys-residues in MT4 are associated with their binding capacity and preference for different metal ions.

关键词： Cucumber CsMT4 Metallothionein Metal-binding properties Heterologous expression

TAK1 is activated by TGF-β signaling and controls axonal growth during brain development

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Journal of Molecular Cell Biology 2014年第4期8卷 349-351页

作者： jingwen yu Feng Zhang Shuo Wang Yongqing Zhang Ming Fan Zhiheng Xu Department of Neuroscience Capital Medical University Beijing 100069 China State Key Laboratory of Molecular Devetopmental Biotogy Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Institute of Basic Medical Sciences Beijing 100850 China Parkinson's Disease Center Beijing institute for Brain Disorders Beijing 100053 China

Mammalian central nervous system neurons show asymmetry during early brain development that defines the elaborate function of neural circuitry （Kriegstein and Noctor, 2004）. Many intracellular signaling pathways, which are important for the transition to the polarized state and the development of axons and dendrites, have been identified （Barnes and Polleux, 2009）. How these pathways are initiated during neuronal development in vivo remained elusive until Yi et al.

关键词：轴突生长发育过程大脑发育 TGF-β 激活控制信令中枢神经系统