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Identification of transporter proteins for PQQ-secretion pathways by transcriptomics and proteomics analysis in Gluconobacter oxydans WSH-003

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Frontiers of Chemical Science and Engineering 2017年第1期11卷 72-88页

作者： Hui Wan Yu Xia Jianghua Li Zhen Kang jingwen zhou School of Biotechnology and Key Laboratory of Industrial B iotechnology Ministry of Education Jiangnan University Wuxi 214122 China Synergetic Innovation of Center of Food Safety and Nutrition Jiangnan University Wuxi 214122 China

Pyrroloquinoline quinone （PQQ） plays a sig- nificant role as a redox cofactor in combination with dehydrogenases in bacteria. These dehydrogenases play key roles in the oxidation of important substrates for the biotechnology industry, such as vitamin C production. While biosynthesis of PQQ genes has been widely studied, PQQ-transport mechanisms used both two-dimensional remain unclear. Herein, we fluorescence-difference gel electrophoresis tandem mass spectrometry and RNA sequencing to investigate the effects ofpqqB overexpres- sion in an industrial strain of Gluconobacter oxydans WSH-003. We have identified 73 differentially expressed proteins and 99 differentially expressed genes, a majority of which are related to oxidation-reduction and transport processes by gene ontology analysis. We also described several putative candidate effectors that responded to increased PQQ levels resulting from pqqB overexpression. Furthermore, quantitative PCR was used to verify five putative PQQ-transport genes among different PQQ producing strains, and the results showed that ompW, B932 1930 and B932_2186 were upregulated in all conditions. Then the three genes were over-expressed in G. oxydans WSH-003 and PQQ production were detected. The results showed that extracellular PQQ of B932_1930 （a transporter） and B932_2186 （an ABC transporter perrnease） overexpression strains were enhanced by 1.77- fold and 1.67-fold, respectively. The results suggest that the proteins encoded by PqqB, B932_1930 and B932 2186 might enhance the PQQ secretion process.

关键词： 2D-DIGE pqqB pyrroloquinoline quinone,RNA-Seq Vitamin C

Copper Indium Sulfide Enables Li-CO_(2)Batteries with Boosted Reaction Kinetics and Cycling Stability

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Energy & Environmental Materials 2023年第5期6卷 372-380页

作者： Lin Chen jingwen zhou Junxiang Zhang Guicai Qi Bin Wang Jianli Cheng Institute of Chemical Materials China Academy of Engineering PhysicsMianyang 621900China Yangtze Delta Region Institute Institute of Fundamental and Frontiers SciencesUniversity of Electronic Sciences and Technology of ChinaHuzhou 313001China Institute of Fundamental and Frontiers Sciences University of Electronic Sciences and Technology of ChinaChengdu 611731China Department of Chemistry City University of Hong KongKowloon Hong Kong China

High energy density Li-CO_(2)batteries have attracted much attention owing to the"two birds with one stone"feature in fixing greenhouse gas CO_(2)and providing renewable ***,poor reversibility of the discharge product Li_(2)CO_(3)is one of the main problems that limit its application,resulting in poor cycling stability and severe ***,copper indium sulfide(CIS),a semiconducting non-precious metal sulfide,is fabricated as cathode catalysts for high-performance Li-CO_(2)*** with the synergistic effect of bimetallic valence bonding and coordinated electron transfer,Li-CO_(2)batteries using CIS cathodes exhibit high full specific discharge capacity,excellent rate capability and cycle stability,namely it delivers a high specific full discharge capacity of 8878μAh cm^(-2),runs steadily from 10 to 100μA cm^(-2),and performs a stable long-term cycling behavior(>1050 h)under a high energy efficiency of 84%and a low charge voltage of approximately 3.4 V at 20μA cm^(-2)within 100μAh cm^(-2).In addition,a flexible Li-CO_(2)pouch cell is constructed to reveal the potential of employing CIS to fabricate flexible high energy storage devices in practical *** work shows a promising development pathway toward next-generation sustainable energy storage devices.

关键词： copper indium sulfides cycling stability Li-CO_(2)batteries reaction kinetics

Optimization of citrinin production by endophytic Penicillium citrinum isolated from Ziziphus jujuba

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Systems Microbiology and Biomanufacturing 2022年第4期2卷 634-642页

作者： Hengrui zhou Song Gao Weizhu Zeng Zhenshan Deng jingwen zhou Science Center for Future Foods Jiangnan University1800 Lihu RoadWuxi 214122JiangsuChina Key Laboratory of Industrial Biotechnology Ministry of Education and School of BiotechnologyJiangnan University1800 Lihu RoadWuxi 214122JiangsuChina College of Life Sciences Yan’an University580 Shengdi RoadYan’an 716000ShanxiChina Engineering Research Center of Ministry of Education On Food Synthetic Biotechnology Jiangnan University1800 Lihu RoadWuxi 214122JiangsuChina

Citrinin is a common mycotoxin produced by different *** considerable endeavors have been made to increase citrinin output to meet the requirements of research and industrial needs,few methods are effective,owing to potential safety concerns and technical *** this study,a blue fungus that produces a yellow pigment was isolated from Ziziphus *** strain was then identified as Penicillium citrinum and named *** liquid chromatography was used to extract the yellow *** LC-MS and NMR assays showed that it was *** enhance the citrinin pro-duction of this strain,the culture medium was optimized through orthogonal experiments,as well as batch and fed-batch *** final titer and productivity of citrinin reached 9.62 g/L and 0.1 g/(L×h),*** high titer achieved indicates the possibility of large-scale production of high purity citrinin for low-cost supply for both academic and diagnostic analysis standard.

关键词： Mycotoxin Penicillium citrinum Endophytic fungus Process optimization

Metal chalcogenides for potassium storage

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InfoMat 2020年第3期2卷 437-465页

作者： jingwen zhou Ye Liu Shilin Zhang Tengfei zhou Zaiping Guo Institute for Superconducting and Electronic Materials(ISEM) Australian Institute for Innovative Materials(AIIM)School of MechanicalMaterialsMechatronics and Biomedical EngineeringUniversity of WollongongWollongongAustralia Institute of Chemical Materials China Academy of Engineering PhysicsMianyangSichuanPeople's Republic of China

Potassium-based energy storage technologies,especially potassium ion batteries(PIBs),have received great interest over the past decade.A pivotal challenge facing high-performance PIBs is to identify advanced electrode materials that can store the large-radius K+ions,as well as to tailor the various thermodynamic *** chalcogenides are one of the most promising anode materials,having a high theoretical specific capacity,high in-plane electrical conductivity,and relatively small volume change on charge/***,the development of metal chalcogenides for PIBs is still in its infancy because of the limited choice of high-performance electrode ***,numerous efforts have been made to conquer this *** this article,we overview potassium storage mechanisms,the technical hurdles,and the optimization strategies for metal chalcogenides and highlight how the adjustment of the crystalline structure and choice of the electrolyte affect the electrochemical performance of metal-chalcogenide-based electrode *** potential potassium-based energy storage systems to which metal chalcogenides can be applied are also ***,future research directions focusing on metal chalcogenides for potassium storage are proposed.

关键词： energy storage metal chalcogenides modification strategies nanocomposites potassium ion batteries

Microbial chassis design and engineering for production of amino acids used in food industry

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Systems Microbiology and Biomanufacturing 2023年第1期3卷 28-48页

作者： Jianli Wang Wenjian Ma jingwen zhou Xiaoyuan Wang State Key Laboratory of Food Science and Technology Jiangnan University1800 Lihu AvenueWuxi 214000China International Joint Laboratory on Food Safety Jiangnan UniversityWuxi 214122China Science Center for Future Foods Jiangnan UniversityWuxi 214122China Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China

Rational microbial chassis design and engineering for improving production of amino acids have attracted a considerable attention.l-glutamate,l-lysine,l-threonine and l-tryptophan are the main amino acids demanded in the food *** metabolic engineering and synthetic biology engineering generally are believed as the comprehensive engineering approaches to obtain rationally designed strains and construct high-performance platforms for amino *** strate-gies focus on microbial chassis characterization optimization,precise metabolic engineering such as promoter engineer-ing,modular pathway engineering,transporter engineering,and dynamic switch systems application,and global genome streamline engineering to reduce cell *** this review,we summarized the efficient engineering strategies to optimize Corynebacterium glutamicum and Escherichia coli cell factories for improving the production of l-glutamate,l-lysine,l-threonine,and l-tryptophan.

关键词： Microbial chassis Amino acids Food industries Synthetic biology Metabolic engineering Escherichia coli Corynebacterium glutamicum l-Glutamate l-Lysine l-Threonine l-Tryptophan

Toward fine-tuned metabolic networks in industrial microorganisms

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Synthetic and Systems Biotechnology 2020年第2期5卷 81-91页

作者： Ning Li Weizhu Zeng Sha Xu jingwen zhou Key Laboratory of Industrial Biotechnology Ministry of Education and School of BiotechnologyJiangnan University1800 Lihu RoadWuxiJiangsu214122China National Engineering Laboratory for Cereal Fermentation Technology Jiangnan University1800 Lihu RoadWuxiJiangsu214122China Jiangsu Provisional Research Center for Bioactive Product Processing Technology Jiangnan University1800 Lihu RoadWuxiJiangsu214122China

There are numerous microorganisms in nature capable of synthesizing diverse useful compounds;however,these natural microorganisms are generally inefficient in the production of target products on an industrial scale,relative to either chemical synthesis or extraction *** achieve industrial production of useful compounds,these natural microorganisms must undergo a certain degree of mutation or effective fine-tuning *** review describes how to achieve an ideal metabolic fine-tuned process,including static control strategies and dynamic control *** static control strategies mainly focus on various matabolic engineering strategies,including protein engineering,upregulation/downregulation,and combinatrorial control of these metabolic engineering strategies,to enhance the flexibility of their application in fine-tuned metabolic ***,we focus on the dynamic control strategies for fine-tuned metabolic *** design principles derived would guide us to construct microbial cell factories for various useful compounds.

关键词： Fine-tuned regulation Protein engineering Upregulation Downregulation Dynamic regulation

Information transmission through parallel multi-task-based recognition of high-resolution multiplexed orbital angular momentum

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Frontiers of physics 2024年第5期19卷 167-177页

作者： jingwen zhou Yaling Yin Jihong Tang Yong Xia Jianping Yin State key laboratory of precision spectroscopy School of Physics and Electronic ScienceEast China Normal UniversityShanghai 200241China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China NYU-ECNU Institute of Physics at NYU Shanghai Shanghai 200062China

Orbital angular momentums(OAMs)greatly enhance the channel capacity in free-space optical ***,demodulation of superposed OAM to recognize them separately is always difficult,especially upon multiplexing more *** this work,we report a directly recognition of multiplexed fractional OAM modes,without separating them,at a resolution of 0.1 with high accuracy,using a multi-task deep learning(MTDL)model,which has not been reported ***,two-mode,four-mode,and eight-mode superposed OAM beams,experimentally generated with a hologram carrying both phase and amplitude information,are well recognized by the suitable MTDL *** applications in information transmission are presented:the first is for 256-ary OAM shift keying via multiplexed fractional OAMs;the second is for OAM division multiplexed information transmission in an eightfold *** encouraging results will expand the capacity in future free-space optical communication.

关键词： information transmission orbital angular momentum multitask deep learning holographic multiplexing structured light

CRISPR/Cpf1–FOKI-induced gene editing in Gluconobacter oxydans

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Synthetic and Systems Biotechnology 2024年第2期9卷 369-379页

作者： Xuyang Wang Dong Li Zhijie Qin Jian Chen jingwen zhou Engineering Research Center of Ministry of Education on Food Synthetic Biotechnology Jiangnan University1800 Lihu RoadWuxiJiangsu214122China Science Center for Future Foods Jiangnan University1800 Lihu RoadWuxiJiangsu214122China Key Laboratory of Industrial Biotechnology Ministry of Education and School of BiotechnologyJiangnan University1800 Lihu RoadWuxiJiangsu214122China Jiangsu Province Engineering Research Center of Food Synthetic Biotechnology Jiangnan UniversityWuxi214122China

Gluconobacter oxydans is an important Gram-negative industrial microorganism that produces vitamin C and other products due to its efficient membrane-bound dehydrogenase *** incomplete oxidation system has many crucial industrial ***,it also leads to slow growth and low biomass,requiring further metabolic modification for balancing the cell growth and incomplete oxidation *** a non-model strain,*** lacks efficient genome editing tools and cannot perform rapid multi-gene editing and complex metabolic network *** the last 15 years,our laboratory attempted to deploy multiple CRISPR/Cas systems in different *** strains and found none of them as *** this study,Cpf1-based or dCpf1-based CRISPRi was constructed to explore the targeted binding ability of Cpf1,while Cpf1–FokI was deployed to study its nuclease activity.A study on Cpf1 found that the CRISPR/Cpf1 system could locate the target genes in *** but lacked the nuclease cleavage ***,the CRISPR/Cpf1–FokI system based on FokI nuclease was ***-gene knockout with efficiency up to 100%and double-gene iterative editing were achieved in *** this system,AcrVA6,the anti-CRISPR protein of *** was discovered for the first time,and efficient genome editing was realized.

关键词： Cell growth CRISPRi Anti-CRISPR protein Multi-gene editing Vitamin C

Yanggan Jiangmei Formula alleviates hepatic inflammation and lipid accumulation in non-alcoholic steatohepatitis by inhibiting the NF-κB/NLRP3 signaling pathway

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Chinese Journal of Natural Medicines 2024年第3期22卷 224-234页

作者： WU Yuanyuan zhou jingwen ZUO Xinchen KUANG Yufeng SUN Lixia ZHANG Xiaolong The First Clinical Medical College Nanjing University of Chinese MedicineNanjing 210023China The Affiliated Hospital of Nanjing University of Chinese Medicine Jiangsu Province Hospital of Chinese MedicineNanjing 210029China

The role of NF-κB and the NLRP3 inflammasome in the chronic inflammatory microenvironment of non-alcoholic steatohepatitis(NASH)has been posited as *** Yanggan Jiangmei Formula(YGJMF)has shown promise in ameliorating hepatic steatosis in NASH patients,yet its pharmacological mechanisms remain largely *** study was conducted to investigate the efficacy of YGJMF in NASH and to elucidate its pharmacological *** simulate NASH both in vivo and in vitro,high-fat-diet(HFD)rats and HepG2 cells stimulated with free fatty acids(FFAs)were *** severity of liver injury and lipid deposition was assessed using serum indicators,histopathological staining,micro-magnetic resonance imaging(MRI),and the liver-tomuscle signal intensity ratio(SIRL/M).Furthermore,a combination of enzyme-linked immunosorbent assay(ELISA),immunohistochemistry(IHC),immunofluorescence,real-time quantitative polymerase chain reaction(RT-qPCR),and Western blotting analyses was employed to investigate the NF-κB/NLRP3 signaling pathway and associated cytokine *** results from liver pathology,MRI assessments,and biochemical tests in rat models demonstrated YGJMF’s significant effectiveness in reducing liver damage and lipid ***,YGJMF markedly reduced hepatocyte inflammation by downregulating inflammatory cytokines in both liver tissue and ***,YGJMF was found to disrupt NF-κB activation,consequently inhibiting the assembly of the NLRP3 inflammasome in both the in vitro and in vivo *** preliminary findings of this study suggest that YGJMF may alleviate hepatic steatosis and inhibit the NF-κB/NLRP3 signaling pathway,thereby exerting anti-inflammatory effects in NASH.

关键词： Non-alcoholic steatohepatitis Nuclear factor kappa B NLRP3 inflammasome Yanggan Jiangmei Formula