检索结果-南通市图书馆

Genome-wide identification of RNA editing in seven porcine tissues by matched DNA and RNA high-throughput sequencing

在线全文

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2019年第2期10卷 339-352页

作者： yuebo Zhang Longchao Zhang jingwei yue Xia Wei Ligang Wang Xin Liu Hongmei Gao Xinhua Hou Fuping Zhao Hua Yan Lixian Wang Key Laboratory of Animal(Poultry)Genetics Breeding and Reproduction Ministry of Agriculture Institute of Animal Science Chinese Academy of Agricultural SciencesBeijing 100193China

Background: RNA editing is a co/posttranscriptional modification mechanism that increases the diversity of transcripts, with potential functional consequences. The advent of next-generation sequencing technologies has enabled the identification of RNA edits at unprecedented throughput and resolution. However, our knowledge of RNA editing in swine is still ***: Here, we utilized RES-Scanner to identify RNA editing sites in the brain, subcutaneous fat, heart, liver,muscle, lung and ovary in three 180-day-old Large White gilts based on matched strand-specific RNA sequencing and whole-genome resequencing datasets. In total, we identified 74863 editing sites, and 92.1% of these sites caused adenosine-to-guanosine(A-to-G) conversion. Most A-to-G sites were located in noncoding regions and generally had low editing levels. In total, 151 A-to-G sites were detected in coding regions(CDS), including 94 sites that could lead to nonsynonymous amino acid changes. We provide further evidence supporting a previous observation that pig transcriptomes are highly editable at PRE-1 elements. The number of A-to-G editing sites ranged from 4155(muscle) to 25001(brain) across the seven tissues. The expression levels of the ADAR enzymes could explain some but not all of this variation across tissues. The functional analysis of the genes with tissuespecific editing sites in each tissue revealed that RNA editing might play important roles in tissue ***, more pathways showed significant enrichment in the fat and liver than in other tissues, while no pathway was enriched in the ***: This study identified a total of 74863 nonredundant RNA editing sites in seven tissues and revealed the potential importance of RNA editing in tissue function. Our findings largely extend the porcine editome and enhance our understanding of RNA editing in swine.

关键词： ADAR A-to-G High-throughput sequencing RNA editing Swine

The landscape of chromatin accessibility in skeletal muscle during embryonic development in pigs

在线全文

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2021年第4期12卷 1281-1295页

作者： jingwei yue Xinhua Hou Xin Liu Ligang Wang Hongmei Gao Fuping Zhao Lijun Shi Liangyu Shi Hua Yan Tianyu Deng Jianfei Gong Lixian Wang Longchao Zhang Key Laboratory of Animal(Poultry)Genetics Breeding and Reproduction Ministry of AgricultureInstitute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193China

Background:The development of skeletal muscle in pigs during the embryonic stage is precisely regulated by transcriptional mechanisms,which depend on chromatin ***,how chromatin accessibility plays a regulatory role during embryonic skeletal muscle development in pigs has not been *** gain insight into the landscape of chromatin accessibility and the associated genome-wide transcriptome during embryonic muscle development,we performed ATAC-seq and RNA-seq analyses of skeletal muscle from pig embryos at 45,70 and 100 days post coitus(dpc).Results:In total,21,638,35,447 and 60,181 unique regions(or peaks)were found across the embryos at 45 dpc(LW45),70 dpc(LW70)and 100 dpc(LW100),*** than 91%of the peaks were annotated within−1 kb to 100 bp of transcription start sites(TSSs).First,widespread increases in specific accessible chromatin regions(ACRs)from embryos at 45 to 100 dpc suggested that the regulatory mechanisms became increasingly complicated during embryonic ***,the findings from integrated ATAC-seq and RNA-seq analyses showed that not only the numbers but also the intensities of ACRs could control the expression of associated ***,the motif screening of stage-specific ACRs revealed some transcription factors that regulate muscle developmentrelated genes,such as MyoG,Mef2c,and *** potential transcriptional repressors,including E2F6,OTX2 and CTCF,were identified among the genes that exhibited different regulation trends between the ATAC-seq and RNA-seq ***:This work indicates that chromatin accessibility plays an important regulatory role in the embryonic muscle development of pigs and regulates the temporal and spatial expression patterns of key genes in muscle development by influencing the binding of transcription *** results contribute to a better understanding of the regulatory dynamics of genes involved in pig embryonic skeletal muscle development.

关键词： Chromatin accessibility Embryo Pig Skeletal muscle Transcriptome

“One stone, two birds”: Salt template enabling porosity engineering and single metal atom coordinating toward high-performance zinc-ion capacitors

在线全文

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2025年第1期100卷 637-645页

作者： Chunliu Zhu Huanyu Liang Ping Li Chenglong Qiu Jingyi Wu jingwei Chen Weiqian Tian yue Zhu Zhi Li Huanlei Wang School of Materials Science and Engineering Ocean University of China Department of Chemical and Materials Engineering University of Alberta

Zinc-ion hybrid capacitors （ZIHCs） have received increasing attention as energy storage devices owing to their low cost,high safety,and environmental ***,their progress has been hampered by low energy and power density,as well as unsatisfactory long-cycle stability,mainly due to the lack of suitable electrode *** this context,we have developed manganese single atoms implanted in nitrogen-doped porous carbon nanosheets （MnSAs/NCNs） using a metal salt template method as cathodes for *** metal salt serves a dual purpose in the synthesis process:It facilitates the uniform dispersion of Mn atoms within the carbon matrix and acts as an activating agent to create the porous *** applied in ZIHCs,the MnSAs/NCNs electrode demonstrates exceptional performance,including a high capacity of 203 m Ah g-1,an energy density of 138 Wh kg-1at 68 W kg-1,and excellent cycle stability with 91%retention over 10,000 *** calculations indicate that the introduced Mn atoms modulate the local charge distribution of carbon materials,thereby improving the electrochemical *** work demonstrates the significant potential of carbon materials with metal atoms in zinc-ion hybrid capacitors,not only in enhancing electrochemical performance but also in providing new insights and methods for developing high-performance energy storage devices.

关键词： Zinc ion hybrid capacitors Cathodes Carbon materials Heteroatoms Single atoms

Genome-wide search for candidate genes determining vertebrae number in pigs

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Agricultural Science and Engineering 2017年第3期4卷 327-334页

作者： Longchao ZHANG jingwei yue Xin LIU Jing LIANG Kebin ZHAO Hua YAN Na LI Lei PU yuebo ZHANG Huibi SHI Ligang WANG Lixian WANG Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture of China Institute of Animal ScienceChinese Academy of Agricultural Sciences

Longer porcine carcasses may be expected to have more vertebrae. Therefore, vertebrae number in pigs is an economically important trait. To examine the genetic basis of this trait, we genotyped 578 F-2 Large White x Minzhu pigs using the Porcine SNP60K BeadChip. A genome-wide association study (GWAS) identified 36 significant single nucleotide polymorphisms (SNPs) on the chromosomes SSC1 (294.28-300.32 Mb) and SSC7 (102.22-109.39 Mb). A 6.04-Mb region that contained all 13 significant SNPs on SSC1 also contained the gene NR6A1, previously reported to influence the number of vertebrae in pigs. However, the reported putative casual mutation of NR6A1 c. 748C > T showed no genome-wide significant association with the trait, suggesting it was not a causal mutation in our population. The remaining 23 significant SNPs on SSC7 were concentrated in a 7.17-Mb region, which was within a quantitative trait locus interval for number of vertebrae. TMED10 was the closest gene to the most significant SNP and might be a candidate. Haplotype sharing and block analysis refined the QTL to an interval of about 3 Mb containing 29 candidate genes. Of these 29 genes, the previously reported possible casual mutation of VRTN g. 19034A > C was not found to be a causal mutation in our population. Exploration of these genes via additional genetic and functional studies in mammals revealed that TGF beta 3 could be a good candidate on SSC7. A mutation of TGF beta 3 c. 1749G > A was detected by GWAS and could be proposed as a candidate causal mutation, or as closely linked to a causal mutation, for the number of vertebrae in pigs.

关键词： genome-wide association study number of vertebrae pig SSC7 TGF beta 3

可持续发展理念下饲料企业内部控制的优化策略

在线全文

学校读者我要写书评

暂无评论

中国饲料 2023年第10期 86-89页

作者：胡甜岳经纬贵州广播电视大学安顺市分校贵州安顺561000

饲料企业作为国民经济的重要组成部分,不仅能为畜牧养殖业提供充足的能量和营养支持,而且会直接影响农业产业经济的可持续发展。鉴于饲料企业的重要性,农业农村部于2013年颁布《饲料质量安全管理规范》,要求饲料企业从原料采购、生产加... 详细信息

饲料企业作为国民经济的重要组成部分,不仅能为畜牧养殖业提供充足的能量和营养支持,而且会直接影响农业产业经济的可持续发展。鉴于饲料企业的重要性,农业农村部于2013年颁布《饲料质量安全管理规范》,要求饲料企业从原料采购、生产加工、产品质量控制、产品贮存、物流运输等环节加强管理,切实保障饲料产品的质量和安全。面对这一要求,要想规范饲料企业的生产管理行为,必须通过内部控制的方式加强企业管理,从而实现饲料企业的可持续发展。

关键词：饲料企业内部控制问题对策可持续发展

在线全文

学校读者我要写书评

暂无评论

建设理实一体化的综合布线实训中心

辽宁省交通高等专科学校学报 2008年第4期10卷 109-110页

作者：岳经伟辽宁省交通高等专科学校辽宁沈阳110122

综合布线实训中心是对学生进行专业岗位技能培训的重要场所,是高职高专教育的基本硬件,直接影响教学质量与教学水平。建设理实一体化的综合布线实训中心可以给学生一个仿真的实训环境,在这里可以边学边练边操作,很快掌握综合布线的各项... 详细信息

综合布线实训中心是对学生进行专业岗位技能培训的重要场所,是高职高专教育的基本硬件,直接影响教学质量与教学水平。建设理实一体化的综合布线实训中心可以给学生一个仿真的实训环境,在这里可以边学边练边操作,很快掌握综合布线的各项实用技能,实现课堂教学与未来工作岗位间的"短距离对接"。

关键词：综合布线理实一体化实训

基于加权基因共表达网络分析阿尔茨海默病相关的核心基因

在线全文

学校读者我要写书评

暂无评论

南方医科大学学报 2021年第12期41卷 1752-1762页

作者：薛继国刘静耿淼岳敬伟贺浩宸范皎中国人民解放军军事科学院军事医学研究院辐射医学研究所北京100850 中国人民解放军总医院第二医学中心老年医学研究所国家老年疾病临床医学研究中心北京100853

目的采用加权基因共表达网络分析(WGCNA)探索阿尔茨海默病(AD)相关的差异基因模块及其枢纽基因,并对差异基因模块进行生物功能注释。方法从GEO数据库下载转录组测序数据,根据基因的相关性,当关联系数阈值设定为0.85时,参数β=8,以此构... 详细信息

目的采用加权基因共表达网络分析(WGCNA)探索阿尔茨海默病(AD)相关的差异基因模块及其枢纽基因,并对差异基因模块进行生物功能注释。方法从GEO数据库下载转录组测序数据,根据基因的相关性,当关联系数阈值设定为0.85时,参数β=8,以此构建基因共表达网络;采用Pearson相关性检验计算模块基因与临床表型相关性,筛选出与AD显著相关的基因模块,根据模块内的连接性筛选枢纽基因;利用GO功能富集分析和KEGG通路分析对模块进行功能注释。进一步建立β-淀粉样蛋白(Aβ1-42)诱导SH-SY5Y细胞损伤模型,在模型组和对照组中检测枢纽基因的表达水平。结果根据基因表达的相关性,共构建了10个基因共表达模块,其中brown和turquoise模块与AD组显著相关(brown:r=0.66,P<0.001;turquoise:r=-0.68,P<0.001);结果显示48个基因在共表达网络中处于核心地位;通过生物注释功能发现,两模块中的基因主要富集在DNA损伤修复通路和代谢相关通路等生物学过程中。基因的差异表达分析显示,DNASE1、TEKT2、MTSS1L等基因在AD组中高表达,ACP2、LANCL2、GMPR2等基因在AD组中低表达;体外实验进一步验证了在Aβ1-42诱导的SH-SY5Y细胞损伤过程中DNASE1、TEKT2、MTSS1L表达上调(P<0.01),ACP2、LANCL2、GMPR2表达下调(P<0.01)。结论brown和turquoise模块与AD高度相关,并从模块中筛选出MTSS1L、GMPR2、ACP2、ACTG1、LANCL2等枢纽基因,可能通过调节DNA损伤和修复参与AD发病机制。

关键词：阿尔茨海默病加权基因共表达网络分析枢纽基因 DNA损伤修复

Magnetron sputtered nickel oxide with suppressed interfacial defect states for efficient inverted perovskite solar cells

在线全文

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2025年第1期100卷 348-355页

作者： Guoqiang Ma Qin Tan Zhaoning Li jingwei Xiu Jiafeng Wang Tianle Cheng Dong He Qiang Sun Xuhang Ma Francesco Lamberti Zhubing He Department of Materials Science and Engineering Institute of Innovative Materials (I2M) Shenzhen Key Laboratory of Full Spectral Solar Electricity Generation (FSSEG)Southern University of Science and Technology (SUSTech) Core Research Facilities Southern University of Science and Technology Department of Chemical Sciences Department of Industrial Engineering University of Padova

Widely used spin-coated nickle oxide （NiOx） based perovskite solar cells often suffer from severe interfacial reactions between the NiOxand adjacent perovskite layers due to surface defect states,which inherently impair device performance in a long-term view,even with surface molecule *** this study,we developed high-quality magnetron-sputtered NiOxthin films through detailed process optimization,and compared systematically sputtered and spin-coated NiOxthin film surfaces from materials to *** sputtered NiOxfilms exhibit improved crystallinity,smoother surfaces,and significantly reduced Ni3+or Ni vacancies compared to their spin-coated ***,the interface between the perovskite and sputtered NiOxfilm shows a substantially reduced density of defect *** solar cells （PSCs） fabricated with our optimally sputtered NiOxfilms achieved a high power conversion efficiency （PCE） of up to 19.93%and demonstrated enhanced stability,maintaining 86.2% efficiency during 500 h of maximum power point tracking under one standard sun ***,with the surface modification using (4-(2,7-dibromo-9,9-dimethylacridin-10（9H）-yl)butyl)p hosphonic acid （DMAcPA）,the device PCE was further promoted to 23.07%,which is the highest value reported for sputtered NiOxbased PSCs so far.

关键词： Perovskite

脊椎动物胚胎期脊椎的形成及信号通路调控机制

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

畜牧兽医学报 2021年第6期52卷 1461-1470页

作者：刘倩岳静伟牛乃琪王立贤张龙超中国农业科学院北京畜牧兽医研究所北京100193

脊椎是哺乳动物整个躯体的支柱,具有较高的遗传力,且可对胴体长产生重要影响。不同物种之间的脊椎数量互不相同,甚至同一物种如猪、羊等物种内也存在变异。脊椎数主要受胚胎体节发育时期体节数量的影响,为了进一步探究脊椎形成和数量变... 详细信息

脊椎是哺乳动物整个躯体的支柱,具有较高的遗传力,且可对胴体长产生重要影响。不同物种之间的脊椎数量互不相同,甚至同一物种如猪、羊等物种内也存在变异。脊椎数主要受胚胎体节发育时期体节数量的影响,为了进一步探究脊椎形成和数量变异的调控机制,本文综述了脊椎的胚胎发育过程、重要中间结构体节的形成和调控因素,旨在阐明脊椎形成和发育的调控机制,进而为经济动物脊椎数的选育改良提供理论依据和研究思路。

关键词：脊椎胚胎发育体节信号通路