检索结果-南通市图书馆

Mimotope peptide modified pompon mum-like magnetic microparticles for precise recognition,capture and biotransformation analysis of rituximab in biological fluids

引用

Acta Pharmaceutica Sinica B 2024年第3期14卷 1317-1328页

作者： Jiawen Yang Aixuan Zhou Minyi Li Qiaoxian He jingwei Zhou Jacques Crommen Wentao Wang Zhengjin jiang Qiqin Wang Institute of Pharmaceutical Analysis College of Pharmacy/State Key Laboratory of Bioactive Molecules and Druggability Assessment/International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education(MOE)of ChinaJinan UniversityGuangzhou 510632China Laboratory for the Analysis of Medicines Department of Pharmaceutical SciencesCIRMUniversity of LiegeLiege B-4000Belgium SCIEX Shanghai 200050China

Due to low immobilized ligand density,limited binding capacity,and severe interference from serum proteins,developing ideal peptide-based biomaterials for precise recognition and in vivo analysis of biopharmaceuticals remains a huge challenge.In this study,mimotope peptide modified pompon mum-like biomimetic magnetic microparticles(MMPs,3.8μm)that mimic the specific functionalities of CD20 on malignant B cells were developed for the first time.Benefit from the numerous ligand binding sites(Ni^(2+))on the pompon mum-like MMPs,these novel materials achieved≥10 times higher peptide ligand densities(>2300 mg/g)and antibody binding capacities(1380 mg/g)compared to previous reported biomaterials.Leveraging the high specificity of the mimotope peptide,rituximab can be precisely recognized and enriched from cell culture media or serum samples.We also established an LC-MS/MS method using the MMPs for tracking rituximab biotransformation in patient serum.Intriguingly,deamidation of Asn55 and Asn33,as well as oxidation of Met81 and Met34 were observed at the key complementarity determining regions of rituximab,which could potentially influence antibody function and require careful monitoring.Overall,these versatile biomimetic MMPs demonstrate superior recognition and enrichment capabilities for target antibodies,offering interesting possibilities for biotransformation analysis of biopharmaceuticals in patient serum.

关键词： Therapeutic monoclonal antibody Mimotope peptide Precise recognition Peptide-based biomaterials Biotransformation Patient serum

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Design Principles and Mechanistic Understandings of Non-Noble-Metal Bifunctional Electrocatalysts for Zinc-Air Batteries

引用

Nano-Micro Letters 2024年第9期16卷 13-48页

作者： Yunnan Gao Ling Liu Yi jiang Dexin Yu Xiaomei Zheng Jiayi Wang jingwei Liu Dan Luo Yongguang Zhang Zhenjia Shi Xin Wang Ya‑Ping Deng Zhongwei Chen Power Battery and Systems Research Center State Key Laboratory of CatalysisDalian Institute of Chemical PhysicsDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023People’s Republic of China Department of Chemical Engineering University of WaterlooWaterlooON N2L 3G1Canada Institute of Carbon Neutrality Zhejiang Wanli UniversityNingbo 315100People’s Republic of China College of Materials and Chemistry China Jiliang UniversityHangzhou 310018People’s Republic of China

Zinc-air batteries(ZABs)are promising energy storage systems because of high theoretical energy density,safety,low cost,and abundance of zinc.However,the slow multi-step reaction of oxygen and heavy reliance on noble-metal catalysts hinder the practical applications of ZABs.Therefore,feasible and advanced non-noble-metal elec-trocatalysts for air cathodes need to be identified to promote the oxygen catalytic reaction.In this review,we initially introduced the advancement of ZABs in the past two decades and provided an overview of key developments in this field.Then,we discussed the work-ing mechanism and the design of bifunctional electrocatalysts from the perspective of morphology design,crystal structure tuning,interface strategy,and atomic engineering.We also included theoretical studies,machine learning,and advanced characterization technologies to provide a comprehensive understanding of the structure-performance relationship of electrocatalysts and the reaction pathways of the oxygen redox reactions.Finally,we discussed the challenges and prospects related to designing advanced non-noble-metal bifunctional electrocatalysts for ZABs.

关键词： Zinc-air batteries Bifunctional electrocatalysts Design principles Mechanistic understandings

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于数字散斑的轮轨垂向载荷识别方法研究

引用

铁道科学与工程学报 2024年第2期21卷 851-859页

作者：陈晶伟姜曼杨岳中南大学交通运输工程学院湖南长沙410075 株洲电力机车有限公司大功率交流传动电力机车系统集成国家重点实验室湖南株洲412000

快捷有效地进行轮轨垂向载荷检测对保障轨道车辆的服役可靠性与运行安全具有重要意义。现有轮轨载荷检测方法尽管能实现轮轨垂向载荷的有效检测,但其测量精度受传感器布设方案、系统标定的限制,仅能实现定点检测。因此,引入数字散斑图... 详细信息

快捷有效地进行轮轨垂向载荷检测对保障轨道车辆的服役可靠性与运行安全具有重要意义。现有轮轨载荷检测方法尽管能实现轮轨垂向载荷的有效检测,但其测量精度受传感器布设方案、系统标定的限制,仅能实现定点检测。因此,引入数字散斑图像相关技术,为轮轨垂向载荷的快速、非定点检测提供新的技术方案。为了实现钢轨轨侧应力场的非接触、快速测量,通过左右相机同步采集垂向载荷作用下的钢轨散斑图像序列,并通过图像相关理论模型进行变形前后子区像素点的匹配和应力场计算。为了利用数字散斑检测获得的钢轨轨侧应力分布进行轮轨垂向载荷的识别,提出一种基于极限学习机(ELM)的轮轨垂向载荷识别方法。该方法采用Workbench有限元软件建立钢轨的数值模型,通过数值模拟结果选取应力变化相对敏感的区域作为轮轨垂向载荷检测的兴趣域,基于应力场数据集和对应的载荷数据集设计ELM网络参数,进而实现轮轨垂向载荷的自动识别。为了验证提出的轮轨垂向载荷识别模型的有效性,搭建基于XT-DIC的轮轨垂向载荷数字散斑检测实验平台。实验结果表明,用Y和Z方向模应力输入的方式构建的ELM模型有着最佳的识别性能,其垂向载荷预测误差仅有5.357%。研究结果为轮轨垂向载荷的非定点快速检测提供了新的可靠途径。

关键词：轮轨载荷数字散斑图像钢轨应力 ELM

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Role of Standards for Communicable Disease Prevention and Control in Protecting People’s Health and Safety During COVID-19

引用

China CDC weekly 2020年第42期2卷 823-826页

作者： Lan Feng jingwei jiang Yuan Ma Jinxing Lu National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijingChina

World Standards Day was established by the International Organization for Standardization(ISO)on October 14 to enhance awareness on the significance of international standardization to respond to the needs of businesses,industries,governments and consumers worldwide.Since its rejoining the ISO in 1978,China held various presentations,symposiums,and commemorative events for World Standards Day in major and medium-sized cities nationwide to publicize the role of standardization in societal development and enhance the awareness of standardization among the people.

关键词： nationwide sized awareness

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asymmetric expression of CA2 and CA13 linked to calcification in the bilateral mandibular condyles cause crossed beaks in chickens

引用

Journal of Integrative Agriculture 2024年第7期23卷 2379-2390页

作者： Lei Shi Yanyan Sun Yunlei Li Hao Bai jingwei Yuan Hui Ma Yuanmei Wang Panlin Wang Aixin Ni Linlin jiang Pingzhuang Ge Shixiong Bian Yunhe Zong Jinmeng Zhao Adamu MIsa Hailai HTesfay Jilan Chen State Key Laboratory of Animal Biotech Breeding/Key Laboratory of Animal(Poultry)Genetics Breeding and Reproduction of Ministry of Agriculture and Rural Affairs/Institute of Animal Sciences Chinese Academy of Agricultural SciencesBeijing 100193China College of Animal Science and Technology Hebei Agricultural UniversityBaoding 071001China Joint International Research Laboratory of Agriculture and Agri-Product Safety Ministry of Education/Institutes of Agricultural Science and Technology DevelopmentYangzhou UniversityYangzhou 225009China

Crossed beak is a complex mode of inheritance with prevalence ranging from 0.2 to 7.4%in at least 12 chicken strains worldwide.To reveal the intrinsic factors causing crossed beaks,genes expression patterns in bilateral mandibular condyle between affected and normal birds were characterized by RNA sequencing analysis in the present studies.Crossed beak was induced by short length of unilateral mandibular ramus,and a total of 110 differentially expressed genes were up-or down-regulated in the affected(short)mandibular condyle side as compared to the normal side.Carbonic anhydrase 2(CA2)and Carbonic anhydrase 13(CA13)were enriched in the carbonate dehydratase activity,and high-expressed in mandibular condyle and osteoblasts(P<0.05).However,both were low-expressed in short mandibular condyle side of affected birds(P<0.05).The carbonate dehydratase inhibitor experiments confirmed that there is positive association between the calcification and carbonic anhydrase isoenzymes.Quantitative analysis with cetylpyridinium chloride showed a decrease in calcification when the cells were transfected with an anti-CA13 shRNA.Our research suggested that CA2 and CA13 are down-calcified in shortside mandibular condyle,and caused mandibular ramus to grow slowly.CA2 and CA13 have the critical role in crossed beaks by regulating calcification of mandibular condyle.

关键词： chicken crossed beak carbonic anhydrase calcification CA2 CA13

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Impeding the combination of astrocytic ASCT2 and NLRP3 by talniflumate alleviates neuroinflammation in experimental models of Parkinson's disease

引用

Acta Pharmaceutica Sinica B 2023年第2期13卷 662-677页

作者： Yang Liu Ting Liu Yuanzhang Zhou Wenjie Li Min Wang Nanshan Song Wenbin Zhang jingwei jiang Shengtao Yuan Jianhua Ding Gang Hu Ming Lu jiangsu Key Laboratory of Neurodegeneration Department of PharmacologyNanjing Medical UniversityNanjing 211166China Department of Pharmacology School of Medicine and Holistic Integrative MedicineNanjing University of Chinese MedicineNanjing 210023China Department of Geriatrics Affiliated Brain Hospital of Nanjing Medical UniversityNanjing 210029China Department of Neurosurgery Affiliated Brain Hospital of Nanjing Medical UniversityNanjing 210029China jiangsu Key Laboratory of Drug Screening China Pharmaceutical UniversityNanjing 211198China

Alanine-serine-cysteine transporter 2(ASCT2)is reported to participate in the progression of tumors and metabolic diseases.It is also considered to play a crucial role in the glutamate-glutamine shuttle of neuroglial network.However,it remains unclear the involvement of ASCT2 in neurological diseases such as Parkinson’s disease(PD).In this study,we demonstrated that high expression of ASCT2 in the plasma samples of PD patients and the midbrain of MPTP mouse models is positively correlated with dyskinesia.We further illustrated that ASCT2 expressed in astrocytes rather than neurons significantly upregulated in response to either MPP+or LPS/ATP challenge.Genetic ablation of astrocytic ASCT2 alleviated the neuroinflammation and rescued dopaminergic(DA)neuron damage in PD models in vitro and in vivo.Notably,the binding of ASCT2 to NLRP3 aggravates astrocytic inflammasometriggered neuroinflammation.Then a panel of 2513 FDA-approved drugs were performed via virtual molecular screening based on the target ASCT2 and we succeed in getting the drug talniflumate.It is validated talniflumate impedes astrocytic inflammation and prevents degeneration of DA neurons in PD models.Collectively,these findings reveal the role of astrocytic ASCT2 in the pathogenesis of PD,broaden the therapeutic strategy and provide a promising candidate drug for PD treatment.

关键词： ASCT2 NLRP3 Astrocytes Inflammation Neurodegeneration Drug screening Talniflumate Parkinson’s disease

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

有限视场下的攻击时间和角度多约束制导律

引用

北京理工大学学报 2024年第1期44卷 18-27页

作者：王江刘经纬崔晓曦范卫鹏北京理工大学宇航学院北京100081 中国兵器工业导航与控制技术研究所北京100089 西北工业集团有限公司陕西西安710043

针对精确打击任务存在导引头视场受限这一过程约束的制导问题,提出了一种考虑视场限制以及攻击角度和时间多种约束的制导律.首先,在以比例导引项实现精确命中的基础上,利用最优控制理论,采用弹目距离加权控制能耗形式的指标函数,设计了... 详细信息

针对精确打击任务存在导引头视场受限这一过程约束的制导问题,提出了一种考虑视场限制以及攻击角度和时间多种约束的制导律.首先,在以比例导引项实现精确命中的基础上,利用最优控制理论,采用弹目距离加权控制能耗形式的指标函数,设计了攻击角度控制项,并推导了相应的剩余飞行时间解析预测表达式.其次,基于最优误差动力学方法,设计了攻击时间相关的控制项,保证了攻击时间误差的有限时间收敛.同时,针对导引头视场有限的问题,分析了各控制项对前置角收敛性的影响,利用特定的限制函数确保前置角在整个制导过程中始终处于有效视场内,并给出了前置角的有界性及收敛性证明.数学仿真结果验证了所提出制导律对多约束制导问题的有效性.

关键词：最优控制攻击角度控制攻击时间控制视场约束最优误差动力学

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Artificial intelligence and big data facilitated targeted drug discovery

引用

Stroke & Vascular Neurology 2019年第4期4卷 206-213页

作者： Benquan Liu Huiqin He Hongyi Luo Tingting Zhang jingwei jiang jiangsu Key Lab of Drug Screening China Pharmaceutical UniversityNanjingChina

Different kinds of biological databases publicly available nowadays provide us a goldmine of multidiscipline big data.The Cancer Genome Atlas is a cancer database including detailed information of many patients with cancer.DrugBank is a database including detailed information of approved,investigational and withdrawn drugs,as well as other nutraceutical and metabolite structures.PubChem is a chemical compound database including all commercially available compounds as well as other synthesisable compounds.Protein Data Bank is a crystal structure database including X-ray,cryo-EM and nuclear magnetic resonance protein three-dimensional structures as well as their ligands.On the other hand,artificial intelligence(AI)is playing an important role in the drug discovery progress.The integration of such big data and AI is making a great difference in the discovery of novel targeted drug.In this review,we focus on the currently available advanced methods for the discovery of highly effective lead compounds with great absorption,distribution,metabolism,excretion and toxicity properties.

关键词： metabolism targeted drugs

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

用于新型符号的频偏补偿和解调的算法与电路

引用

哈尔滨工业大学学报 2024年第5期56卷 121-129页

作者：林敏史靖炜丁福建姜帆陈潇特种光纤与光接入网重点实验室(上海大学) 上海201900

为提高传统脉冲位置调制(pulse position modulation,PPM)符号的频谱效率,提出了一种新型码片内4-PPM符号调制方法,在实现1 Gbit/s通信速率的同时,又大大减少所需频谱资源。可在解调时,该符号调制的误码率性能受到发射端时钟和接收端本... 详细信息

为提高传统脉冲位置调制(pulse position modulation,PPM)符号的频谱效率,提出了一种新型码片内4-PPM符号调制方法,在实现1 Gbit/s通信速率的同时,又大大减少所需频谱资源。可在解调时,该符号调制的误码率性能受到发射端时钟和接收端本地时钟之间的频率偏移的极大影响。针对此问题,又提出了一种在模拟域对该符号进行频偏补偿,并实现符号同步和高速数据解调的算法与电路。该电路系统通过消除接收数据和本地时钟的初始相差、提取两者的频偏信息、周期性改变本地时钟的瞬时相位3步实现频偏补偿,并同时在第3步利用本地时钟对接收数据进行解调。为提高相位插值器(phase interpolator,PI)的线性度,本文将延迟锁定环与PI相结合。在2π的插值范围内,实现插值区间32个,插值步长992个,分辨率2.016 ps,最大差分非线性(differential nonlinearity,DNL)0.183°,最大积分非线性(integral nonlinearity,INL)0.325°。此外,本文提出的相位控制算法有效避免了由电流毛刺所引起的输出相位突变。电路基于UMC 40 nm CMOS RF LP工艺进行设计与仿真。仿真结果表明:本文所提出的算法与电路,在典型工艺角下,将接收数据和本地时钟间的50×10^(-6)频率偏差度降至1.03×10^(-6),频偏补偿准确度达到97.94%,并实现1 Gbit/s的解调速率。该方法对高速PPM数据同步与解调具有良好的工程应用价值。

关键词：脉冲位置调制码片内脉冲位置调制符号同步频偏补偿数据解调相位插值器延迟锁定环

来源：

维普期刊数据库

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Big data and artificial intelligence discover novel drugs targeting proteins without 3D structure and overcome the undruggable targets

引用

Stroke & Vascular Neurology 2020年第4期5卷 381-387页

作者： Huiqin He Benquan Liu Hongyi Luo Tingting Zhang jingwei jiang jiangsu Key Lab of Drug Screening China Pharmaceutical UniversityNanjingChina Institute of Pharmacologic Science China Pharmaceutical UniversityNanjingChina

The discovery of targeted drugs heavily relies on three-dimensional(3D)structures of target proteins.When the 3D structure of a protein target is unknown,it is very difficult to design its corresponding targeted drugs.Although the 3D structures of some proteins(the so-called undruggable targets)are known,their targeted drugs are still absent.As increasing crystal/cryogenicelectron microscopy structures are deposited in Protein Data Bank,it is much more possible to discover the targeted drugs.Moreover,it is also highly probable to turn previous undruggable targets into druggable ones when we identify their hidden allosteric sites.In this review,we focus on the currently available advanced methods for the discovery of novel compounds targeting proteins without 3D structure and how to turn undruggable targets into druggable ones.

关键词： structure overcome drugs

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：