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Estimation of cancer cell migration in biomimetic random/oriented collagen fiber microenvironments

Chinese Physics B 2024年第5期33卷 749-756页

作者：姚静如李国强姚喜耀周连杰叶志凯刘艳平郑栋天唐婷宋克纳陈果刘雳宇 Chongqing Key Laboratory of Soft Condensed Matter Physics and Smart Materials College of PhysicsChongqing UniversityChongqing 401331China Chongqing Key Laboratory for Resource Utilization of Heavy Metal Wastewater Aquatic Ecosystems in the Three Gorges Reservoir Region of Chongqing Observation and Research StationChongqing University of Arts and SciencesYongchuan 402160China Wenzhou Institute University of Chinese Academy of SciencesWenzhou 325000China Department of Biomedical Engineering Chongqing University of Posts and TelecommunicationsChongqing 400065China College of Medical Technology and Engineering Henan University of Science and TechnologyHenan 471023China

Increasing data indicate that cancer cell migration is regulated by extracellular matrixes and their surrounding biochemical microenvironment,playing a crucial role in pathological processes such as tumor invasion and ***,conventional two-dimensional cell culture and animal models have limitations in studying the influence of tumor microenvironment on cancer cell ***,the further development of microfluidic technology has provided solutions for the study of such *** utilize microfluidic chip to build a random collagen fiber microenvironment(RFM)model and an oriented collagen fiber microenvironment(OFM)model that resemble early stage and late stage breast cancer microenvironments,*** combining cell culture,biochemical concentration gradient construction,and microscopic imaging techniques,we investigate the impact of different collagen fiber biochemical microenvironments on the migration of breast cancer MDA-MB-231-RFP *** results show that MDA-MB-231-RFP cells migrate further in the OFM model compared to the RFM model,with significant differences ***,we establish concentration gradients of the anticancer drug paclitaxel in both the RFM and OFM models and find that paclitaxel significantly inhibits the migration of MDA-MB-231-RFP cells in the RFM model,with stronger inhibition on the high concentration side compared to the low concentration ***,the inhibitory effect of paclitaxel on the migration of MDA-MB-231-RFP cells in the OFM model is *** findings suggest that the oriented collagen fiber microenvironment resembling the late-stage tumor microenvironment is more favorable for cancer cell migration and that the effectiveness of anticancer drugs is *** RFM and OFM models constructed in this study not only provide a platform for studying the mechanism of cancer development,but also serve as a tool for the initial measurement of drug screening.

关键词： microfluidic chip random collagen fiber microenvironment(RFM) oriented collagen fiber microenvironment(OFM) cancer cell migration

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Biophysical model for high-throughput tumor and epithelial cell co-culture in complex biochemical microenvironments

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Chinese Physics B 2022年第2期31卷 154-163页

作者： Guoqiang Li Yanping Liu jingru yao Kena Song Gao Wang Lianjie Zhou Guo Chen Liyu Liu Chongqing Key Laboratory of Soft Condensed Matter Physics and Smart Materials College of PhysicsChongqing UniversityChongqing 401331China College of Medical Technology and Engineering Henan University of Science and TechnologyHenan 471023China

The in vivo tumor microenvironment is a complex niche that includes heterogeneous physical structures,unique biochemical gradients and multiple cell *** high-fidelity in vitro reconstruction is of fundamental importance to improve current understandings of cell behavior,efficacy predictions and drug *** this study,we have developed a high-throughput biochip with hundreds of composite extracellular matrix(ECM)microchambers to co-culture invasive breast cancer cells(MDA-MB-231-RFP)and normal breast epithelial cells(MCF-10 A-GFP).The composite ECM is composed of type I collagen and Matrigel which provides a heterogeneous microenvironment that is similar to that of in vivo cell ***,the growth factors and drug gradients that involve human epidermal growth factor(EGF),discoidin domain receptor 1(DDR1)inhibitor 7 rh and matrix metalloproteinase inhibitor batimastat allow for the mimicking of the complex in vivo biochemical microenvironment to investigate their effect on the spatial-temporal dynamics of cell *** results demonstrate that the MDA-MB-231-RFP cells and MCF-10 A-GFP cells exhibit different spatial proliferation behaviors under the combination of growth factors and *** on the experimental data,we have also developed a cellular automata(CA)model that incorporated drug diffusion to describe the experimental phenomenon,as well as employed Shannon entropy(SE)to explore the effect of the drug diffusion coefficient on the spatial-temporal dynamics of cell *** results indicate that the uniform cell growth is related to the drug diffusion coefficient,which reveals that the pore size of the ECM plays a key role in the formation of complex biochemical ***,our integrated,biomimetic and high-throughput co-culture platforms,as well as the computational model can be used as an effective tool for investigating cancer pathogenesis and drug development.

关键词： microfluidic high-throughput biochemical gradient cancer

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Nonlinear dynamics of cell migration in anisotropic microenvironment

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Chinese Physics B 2021年第9期30卷 166-177页

作者： Yanping Liu Da He Yang Jiao Guoqiang Li Yu Zheng Qihui Fan Gao Wang jingru yao Guo Chen Silong Lou Liyu Liu Chongqing Key Laboratory of Soft Condensed Matter Physics and Smart Materials College of PhysicsChongqing UniversityChongqing 401331China Spine Surgery Beijing Jishuitan HospitalBeijing 100035China Department of Physics Arizona State UniversityTempeArizona 85287USA Materials Science and Engineering Arizona State UniversityTempeArizona 85287USA Beijing National Laboratory for Condensed Matter Physics and CAS Key Laboratory of Soft Matter Physics Institute of PhysicsChinese Academy of Sciences(CAS)Beijing 100190China Department of Neurosurgery Chongqing University Cancer HospitalChongqing 400030China

Cell migration in anisotropic microenvironment plays an important role in the development of normal tissues and organs as well as neoplasm progression,e.g.,osteogenic differentiation of embryonic stem cells was facilitated on stiffer substrates,indicating that the mechanical signals greatly affect both early and terminal differentiation of embryonic stem ***,the effect of anisotropy on cell migration dynamics,in particular,in terms of acceleration profiles which is important for recognizing dynamics modes of cell migration and analyzing the regulation mechanisms of microenvironment in mechanical signal transmission,has not been systematically *** this work,we firstly rigorously investigate and quantify the differences between persistent random walk and anisotropic persistent random walk models based on the analysis of cell migration trajectories and velocity auto-covariance function,both qualitatively and ***,we introduce the concepts of positive and negative anisotropy based on the motility parameters to study the effect of anisotropy on acceleration profiles,especially the nonlinear decrease and non-monotonic *** particularly elaborate and discuss the mechanisms,and physical insights of non-monotonic behaviors in the case of positive anisotropy,focusing on the force exerted on migrating ***,we analyze two types of in vitro cell migration experiments and verify the universality of nonlinear decrease and the consistence of non-monotonic behaviors with numerical *** conclude that the anisotropy of microenvironment is the cause of the non-monotonic and nonlinear dynamics,and the anisotropic persistent random walk can be as a suitable tool to analyze in vitro cell migration with different combinations of motility *** analysis provides new insights into the dynamics of cell migration in complex microenvironment,which also has implications in tissue engineering and cancer research.

关键词： cell migration nonlinear behavior motility parameter acceleration profile anisotropic microenvironment

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浅析小水电工程的投资控制

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中国水能及电气化 2007年第11期 21-22,26页

作者：姚静如福建省光泽县水利局福建光泽354100

小水电工程投资是一项综合性、专业性、政策性的活动,涉及面广,贯穿于工程建设的各个阶段。如何搞好水电施工项目成本管理,直接涉及到企业能否在市场竞争中立于不败之地,不断稳步发展的重要问题。有鉴于此,为实现最大的投资效益,本文作... 详细信息

小水电工程投资是一项综合性、专业性、政策性的活动,涉及面广,贯穿于工程建设的各个阶段。如何搞好水电施工项目成本管理,直接涉及到企业能否在市场竞争中立于不败之地,不断稳步发展的重要问题。有鉴于此,为实现最大的投资效益,本文作者以多年从事小水电工程的经验,浅析几点看法。

关键词：小水电投资控制

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实施小水电代燃料生态工程实现人与自然和谐发展

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中国水能及电气化 2007年第3期 34-35,37页

作者：姚静如福建省光泽县水利局福建光泽354100

光泽县是全国首批100个初级农村电气化达标县,县内有生态公益林保护区40206hm2。发展小水电代燃料生态工程是真正把光泽县建成现代化小康县的富民工程和德政之举。

关键词：小水电代燃料自然和谐

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Daily consumption of Siraitia grosvenorii alleviated lipid accumulation and colon barrier injury via modulation of gut microbiota in high-fat diet C57BL/6 mice

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Food Science and Human Wellness 2024年

作者： jingru Song Yulu Wei Xiaohua Jiang Hongwei Liu Xiaojie Yan Dianpeng Li Fenglai Lu Guangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization， Guangxi Institute of Botany， Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences State Key Laboratory of Mycology， Institute of Microbiology， Chinese Academy of Sciences， No.1 Beichenxi Road Engineering Research Center of Innovative Traditional Chinese， Zhuang and yao Materia Medica， Ministry of Education， Guangxi University of Chinese Medicine

Siraitia grosvenorii (SG)， a traditional edible medicine in China， has shown potential application in health foods due to multiple bioactivities. However， few comparative studies have investigated the effect of daily intake of SG products by traditional drinking way on alleviating metabolic disorders caused by long-term high fat and high sugar diet (HF-HSD). This study investigated the effects of two commercial SG products (SG juice containing comprehensive compositions and mogrosides extract containing high content mogroside V) supplement on fat content， lipid accumulation， colon barrier and gut microbiota in a 38-week HF-HSD mice trial. Results showed that the SG intervention could decrease the weight gain and body fat content， alleviated lipid accumulation， low-grade inflammation and colon barrier injury. SG juice intake was found to increase the antioxidant enzyme activities of serum glutathione peroxidase (GSH-Px) and liver superoxide dismutase (SOD). 16S rDNA analysis found that SG could restore the gut microbiota with downregulation of harmful bacteria increased by HF-HSD and enhancement of the abundances of beneficial genus. In particular， SG juice significantly enriched taxonomic family Prevotellaceae and genus Alloprevotella. These results indicated that SG might be a functional additive to prevent disease risk caused by excess fat and sugar diet， and active ingredients resulting to the discrepancy of performance especially on gut microbiota are worthy of in-depth investigation.

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在DDMAA存在下钨的极谱催化波及其机理探讨

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福州大学学报 1983年第3期 118-123页

作者：安镜如姚晴洪捷林家骥福州大学化学化工系

本文研究了硫酸－二苯羟乙酰－氯酸钾－甜菜碱体系中钨的极谱催化波的适宜条件．峰电位为－１．１Ｖ（对银电极）．检出根限为３．８×１０－９Ｍ．并应用于测定海水中痕迹量的钨．测定了钨与二苯羟乙酸络合物的组成，结果表明Ｗ：Ｈ... 详细信息

本文研究了硫酸－二苯羟乙酰－氯酸钾－甜菜碱体系中钨的极谱催化波的适宜条件．峰电位为－１．１Ｖ（对银电极）．检出根限为３．８×１０－９Ｍ．并应用于测定海水中痕迹量的钨．测定了钨与二苯羟乙酸络合物的组成，结果表明Ｗ：ＨＡ＝１：１．络合物的稳定常数β＝２．２１×１０４．

关键词：极谱催化波苯羟乙酸 DDMAA

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社区缺血性脑卒中患者二级预防服药依从性认知干预方案的构建及应用研究

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中华护理教育 2024年第9期21卷 1099-1106页

作者：张冰妍李进姚颖何静茹杨惠敏河南科技大学护理学院河南省洛阳市471000

目的构建社区缺血性脑卒中患者二级预防服药依从性认知干预方案,并评价其应用效果。方法基于健康行为互动模式框架,通过文献检索、访谈和专家咨询形成社区缺血性脑卒中患者二级预防服药依从性认知干预方案。于2023年4月—7月,采用方便... 详细信息

目的构建社区缺血性脑卒中患者二级预防服药依从性认知干预方案,并评价其应用效果。方法基于健康行为互动模式框架,通过文献检索、访谈和专家咨询形成社区缺血性脑卒中患者二级预防服药依从性认知干预方案。于2023年4月—7月,采用方便抽样法在洛阳市2所社区选取研究对象,分为对照组(n=34)和试验组(n=35)。对照组采用常规健康教育,试验组采用社区缺血性脑卒中患者二级预防服药依从性认知干预方案,比较两组干预后服药依从性情况、合理用药自我效能、服药信念、健康状况指标。结果试验组服药依从率为94%,高于对照组的服药依从率71%(P<0.05)。试验组干预后的合理用药自我效能量表、服药必要性信念维度得分、血压和低密度脂蛋白胆固醇均优于对照组,差异有统计学意义(P<0.05)。结论社区二级预防服药依从性认知干预方案有利于提高缺血性脑卒中患者的服药依从性认知水平,有利于促进、改善健康结局。

关键词：社区护理缺血性脑卒中二级预防服药依从性认知

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红鳘鱼胶胶原蛋白对D-半乳糖致衰老小鼠皮肤的抗衰老作用研究

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日用化学工业（中英文） 2024年第5期54卷 550-557页

作者：钟碧銮廖静如黄泽敏姚若然杜冰张宇广东官栈营养健康科技有限公司与官栈鱼胶营养安全研究中心广东广州510220 华南农业大学食品学院广东广州510642

以红鳘鱼胶为原料,酸酶法提取胶原蛋白,通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、氨基酸组成分析等方法进行理化性质分析,并以D-半乳糖建立衰老小鼠模型,探究其对小鼠皮肤含水量、羟脯氨酸含量、皮肤病理形态和抗氧化活性的... 详细信息

以红鳘鱼胶为原料,酸酶法提取胶原蛋白,通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、氨基酸组成分析等方法进行理化性质分析,并以D-半乳糖建立衰老小鼠模型,探究其对小鼠皮肤含水量、羟脯氨酸含量、皮肤病理形态和抗氧化活性的影响。结果表明,胶原蛋白由2条α链与1条β链组成,为典型的Ⅰ型胶原蛋白,以甘氨酸、丙氨酸、谷氨酸、脯氨酸为特征氨基酸,分别占总氨基酸含量13.890%,8.601%,7.650%和7.124%,且不含色氨酸;动物实验结果表明,经D-半乳糖造模后,MN组小鼠皮肤粗糙、松弛,皱纹数量增多,相较于CN组皮肤水分含量降低了13.52%,羟脯氨酸含量降低了26.20%,抗氧化能力降低,小鼠皮肤真皮层变薄,胶原纤维、弹性纤维含量降低,部分明显断开;红鳘鱼胶胶原蛋白干预后,能够显著改善由于D-半乳糖造成的小鼠背部皮肤水分流失,增强小鼠皮肤组织氧化应激抵抗力,胶原蛋白组皮肤结构基本恢复,皮肤胶原纤维和弹力纤维含量升高,Ⅰ/Ⅲ型胶原蛋白比例升高了9.79%。综上分析,通过红鳘鱼胶制备的胶原蛋白可延缓D-半乳糖引起的皮肤衰老,可为胶原蛋白在化妆品领域的高值化利用提供依据。

关键词：胶原蛋白电泳氨基酸组成 D-半乳糖抗衰老

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表面活性剂临界胶束浓度对洁面产品性能的影响及优化策略

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中国洗涤用品工业 2024年第4期 27-34页

作者：秦尧钱景茹彭安丽李云张廷志水羊化妆品制造有限公司创新研发中心湖南长沙410000

目前大部分市售洁面产品的核心成分多为表面活性剂,而表面活性剂在其临界胶束浓度附近出现一系列性质的变化会对洁面产品的性能产生影响,其中最明显的就是刺激性和残留。通过简要概述表面活性剂临界胶束浓度与洁面性能的联系,从产品配... 详细信息

目前大部分市售洁面产品的核心成分多为表面活性剂,而表面活性剂在其临界胶束浓度附近出现一系列性质的变化会对洁面产品的性能产生影响,其中最明显的就是刺激性和残留。通过简要概述表面活性剂临界胶束浓度与洁面性能的联系,从产品配方角度出发,对在产品制备中降低表面活性剂临界胶束浓度的常用方法进行了归纳总结,并介绍了常用临界胶束浓度测试方法的机制及其优劣势,为打造优异性能洁面提供可行性的建议,并对其未来发展方向进行展望。

关键词：洁面临界胶束浓度表面活性剂刺激性残留

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