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Expression of the Tribolium castaneum (Coleoptera: Tenebrionidae) hsp83 gene and its relation to oogenesis during ovarian maturation

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Journal of genetics and Genomics 2010年第8期37卷 513-522页

作者： jingjing xu 1, juan shu 1, qin zhang key laboratory of animal genetics and breeding of the ministry of agriculture, college of animal science and technology, china agricultural university, beijing 100193, china key laboratory of animal genetics and breeding of the ministry of agriculture College of Animal Science and Technology China Agricultural University Beijing 100193 China

Heat shock proteins （HSP） can protect organisms and cells from thermal damage. In this study, we cloned the full length cDNA encoding the HSP83 protein （the homologue of HSP90） of Tribolium castaneum（red flour beetle）. The isolated cDNA contains the full coding sequence, a partial 5＇ untranslated region of 55 bp and the complete 3＇ untranslated region. We found the hsp83 gene is located on chromosome 5 of the T. castaneum genome. The predicted HSP83 protein sequence has a high similarity （on average 86.77%） with that of other insect species. The expression of the hsp83 gene in the whole body and in the ovary could be induced with heat stress （40℃ for 1 h） in newly hatched （within 3 h post emergence） and mature （10 days post emergence） beetles. Under normal conditions, the hsp83 expression in the ovary is about 3-fold higher than in the whole body at both stages. No significant difference in hsp83 expression was observed between the two ovarian developmental stages regardless if the beetles were treated with heat shock or not. The expression of the HSP83 protein in the whole body could also be induced with heat stress in newly hatched and mature beetles. However, in the ovary, HSP83 was only expressed in the follicle cells of mature beetles and not in newly hatched beetles, regardless if the beetles were treated with heat shock or not. Furthermore, the females were not able to produce mature oocytes after knock-down of the hsp83 expression by injecting dsRNA. These results suggest that the HSP83 protein is involved in protection against heat stress and could be involved in oogenesis during ovarian maturation of T. castaneum.

关键词： hsp83 cDNA cloning Tribolium castaneum expression heat shock

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Mapping QTLs on BTA6 affecting milk production traits in a Chinese Holstein population

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science Bulletin 2005年第16期 67-72页

作者： CHEN Huiyong1, zhang qin2, WANG Chunkao1, shu juan1, MEI Gui1, YIN Cengceng1, HU Fang1, xu jingjing1, GONG Weijia1, LI Hejun1 & QIU Xiaotian1 1. key laboratory of animal genetics and breeding of the ministry of agriculture, college of animal science and technology, china Agri-cultural university, beijing 100094, china 2. college of animal science and technology, china agricultural Uni-versity, beijing 100094, china

A Chinese Holstein population with daughter design was analyzed using 14 microsatellites covering a map distance of 55.7 cM on chromosome 6 to fine map QTL for five milk production traits. 26 paternal half-sib families with 2356 daughters were involved. Two different approaches, linear regression approach and variance component ap-proach, were employed, with a one-QTL model and two-QTL model fitted. With a one-QTL model, the linear regression approach revealed a QTL near BMS470 with effects on milk yield, fat yield, protein yield, and fat percentage, and another QTL near BMS2460 for protein percentage. The variance component approach confirmed the results of linear regres-sion approach for the three yield traits, with the exception that the QTL for fat yield was mapped to a different position near BMS1242. The 95% confidence intervals resulted from linear regression, obtained by bootstrapping, were generally large, ranging from 31 to 53 cM, whereas the variance com-ponent approach revealed very small confidence intervals, calculated by LOD drop-off method, for the three yield traits, only 4―5 cM. With a two-QTL model, both approaches pro-vided strong evidence for the existence of two QTLs for the three yield traits. Along with the QTLs identified in one-QTL model analyses, the linear regression approach revealed a second QTL near BP7 with effects on all the three yield traits, whereas the variance component approach located the sec-ond QTL near ILSS035, BMS470, and BP7 for the three traits, respectively.

关键词： Chinese Holstein cattle daughter design milk produc-tion trait linear regression approach variance component approach.

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Mapping QTLs on BTA6 affecting milk production traits in a Chinese Holstein population

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Chinese science Bulletin 2005年第16期50卷 1737-1742页

作者： CHEN Huiyong zhang qin WANG Chunkao shu juan MEI Gui YIN Cengceng HU Fang xu jingjing GONG Weijia LI Hejun QIU Xiaotian key laboratory of animal genetics and breeding of the ministry of agriculture College of Animal Science and Technology China Agricultural University Beijing 100094. China college of animal science and technology. china agricultural university Beijing 100094 China

关键词：中国荷兰乳牛乳制品变异结构食品安全

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