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Single-Particle Tracking for the Quantification of Membrane Protein Dynamics in Living Plant Cells

Molecular Plant 2018年第11期11卷 1315-1327页

作者： Yaning Cui Meng Yu Xiaomin Yao jingjing xing Jinxing Lin Xiaojuan Li Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing Forestry UniversityBeijing 100083China College of Biological Sciences &Biotechnology Beijing Forestry UniversityBeijing 100083China Institute of Plant Stress Biology State Key Laboratory of Cotton BiologyDepartment of BiologyHenan UniversityJinming StreetKaifeng 475001China

The plasma membrane is a sophisticated,organized,and highly heterogeneous structure that compartmentalizes cellular processes.To decipher the biological processes involving membrane proteins,it is necessary to analyze their spatiotemporal dynamics.However,it is difficult to directly assess the dynamics and interactions of biomolecules in living cells using traditional biochemical methods.Singleparticle tracking (SPT)methods for imaging and tracking single particles conjugated with fluorescent probes offer an ideal approach to acquire valuable and complementary information about dynamic intracellular processes.SPT can be used to quantitatively monitor the diverse motions of individual particles in living cells.SPT also provides super-spatiotemporal resolution that allows early-stage or rapid response information to be obtained for a better understanding of molecular basis of associated signal transduction processes.More importantly,SPT can be used to detect the motion paths of individual biomolecules in vivo and in situ,thus unveiling the dynamic behavior of the biomolecules that support developmental processes in living cells.In this review,we give an overview of SPT methods,from image acquisition to the detection of single particles,as well as tracking and data analysis.We also discuss recent applications of SPT methods in the field ofplant biology to reveal the complex biological functions of membrane proteins.

关键词： single-particle tracking dynamics stoichiometry dwell time colocalization

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Techniques for detecting protein-protein interactions in living cells:principles, limitations, and recent progress

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Science China(Life Sciences) 2019年第5期62卷 619-632页

作者： Yaning Cui Xi Zhang Meng Yu Yingfang Zhu jingjing xing Jinxing Lin Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing Forestry University College of Biological Sciences & Biotechnology Beijing Forestry University Institute of Plant Stress Biology State Key Laboratory of Cotton Biology Department of Biology Henan University

Detecting protein-protein interactions(PPIs) provides fundamental information for understanding biochemical processes such as the transduction of signals from one cellular location to another; however, traditional biochemical techniques cannot provide sufficient spatio-temporal information to elucidate these molecular interactions in living cells. Over the past decade, several new techniques have enabled the identification and characterization of PPIs. In this review, we summarize three main techniques for detecting PPIs in vivo, focusing on their basic principles and applications in biological studies. We place a special emphasis on their advantages and limitations, and, in particular, we introduced some uncommon new techniques, such as single-molecule FRET(smFRET), FRET-fluorescence lifetime imaging microscopy(FRET-FLIM), cytoskeleton-based assay for protein-protein interaction(CAPPI) and single-molecule protein proximity index(smPPI), highlighting recent improvements to the established techniques. We hope that this review will provide a valuable reference to enable researchers to select the most appropriate technique for detecting PPIs.

关键词： protein-protein interactions in vivo techniques RET PCA co-localization

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Quantification of Membrane Protein Dynamics and Interactions in Plant Cells by Fluorescence Correlation Spectroscopy

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Molecular Plant 2016年第9期9卷 1229-1239页

作者： Xiaojuan Li jingjing xing Zongbo Qiu Qihua He Jinxing Lin College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 China Key Laboratory of Plant Resources Institute of Botany Chinese Academy of Sciences Beijing 100093 China University of Chinese Academy of Sciences Beijing 10049 China College of Life Sciences Henan Normal University Xinxiang 453007 China The Health Science Center Peking University Beijing 100191 China These authors contributed equally to this article.

Deciphering the dynamics of protein and lipid molecules on appropriate spatial and temporal scales may shed light on protein function and membrane organization. However, traditional bulk approaches cannot unambiguously quantify the extremely diverse mobility and interactions of proteins in living cells. Fluores- cence correlation spectroscopy （FCS） is a powerful technique to describe events that occur at the singlemolecule level and on the nanosecond to second timescales; therefore, FCS can provide data on the heterogeneous organization of membrane systems. FCS can also be combined with other microscopy techniques, such as super-resolution techniques. More importantly, FCS is minimally invasive, which makes it an ideal approach to detect the heterogeneous distribution and dynamics of key proteins during development. In this review, we give a brief introduction about the development of FCS and summarize the significant contributions of FCS in understanding the organization of plant cell membranes and the dy- namics and interactions of membrane proteins .We also discuss the potential applications of this technique in plant biology.

关键词： fluorescence correlation spectroscopy microdomain membrane protein dynamics interaction

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Arabidopsis Blue Light Receptor Phototropin 1 Undergoes Blue Light-Induced Activation in Membrane Microdomains

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Molecular Plant 2018年第6期11卷 846-859页

作者： Yiqun Xue jingjing xing Yinglang Wan Xueqin Lv Lusheng Fan Yongdeng Zhang Kai Song Li Wang Xiaohua Wang Xin Deng Frantisek Baluska John M. Christie Jinxing Lin Institute of Botany Chinese Academy of Sciences Beijing 100093 China University of Chinese Academy of Sciences Beijing 100049 China Department of Botany and Plant Sciences University of California Riverside CA 92521 USA Department of Cell Biology Yale University School of Medicine New Haven CT 06520 USA Institute of Cellular and Molecular Botany University of Bonn 53115 Bonn Germany Institute of Molecular Cell and Systems Biology University of Glasgow Bower Building Glasgow G12 8QQ UK College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 China

Phototropin （phot）-mediated signaling initiated by blue light （BL） plays a critical role in optimizing photosyn- thetic light capture at the plasma membrane （PM） in plants. However, the mechanisms underlying the regu- lation of phot activity at the PM in response to BL remain largely unclear. In this study, by single-particle tracking and stepwise photobleaching analysis of photl-GFP proteins we demonstrated that in the dark photl proteins remain in an inactive state and mostly exist as monomers. Dimerization and the diffusion rate of photl-GFP increased in a dose-dependent manner in response to BL. In contrast, BL did not affect the lateral diffusion of kinase-inactive photlD806N-GFP but did enhance its dimerization, suggesting that photl dimerization is independent of phosphorylation. Forster resonance energy transfer-fluorescence life- time imaging microscopy analysis revealed that the interaction between photl-GFP and a marker of sterol- rich lipid environments, AtRem1.3-mCherry, was enhanced with increased time of BL treatment. However, this BL-dependent interaction was not obvious in plants co-expressing phot1D806N-GFP and AtRem1.3- mCherry, indicating that BL facilitates the translocation of functional photl-GFP into AtRem1.3-1abeled microdomains to activate phot-mediated signaling. Conversely, sterol depletion attenuated photl-GFP dynamics, dimerization, and phosphorylation. Taken together, these results indicate that membrane micro- domains act as organizing platforms essential for the proper function of activated photl at the PM.

关键词： photl VA-TIRFM spatiotemporal dynamics blue light signaling membrane microdomains

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术前CT影像组学联合CT及病理特征预测局部进展期食管鳞癌术后早期复发

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中国医学影像技术 2024年第6期40卷 863-868页

作者：邢静静刘译阳周悦詹鹏超王睿柴亚如吕培杰高剑波郑州大学第一附属医院放射科河南郑州450052

目的观察术前CT影像组学联合病理及CT特征预测局部进展期食管鳞癌(LAESCC)早期复发(ER)的价值。方法回顾性分析334例LAESCC,按7∶3比例将患者分为训练集(n=234)或验证集(n=100),对其进行随访,记录术后有无ER(即术后12个月内肿瘤复发)。... 详细信息

目的观察术前CT影像组学联合病理及CT特征预测局部进展期食管鳞癌(LAESCC)早期复发(ER)的价值。方法回顾性分析334例LAESCC,按7∶3比例将患者分为训练集(n=234)或验证集(n=100),对其进行随访,记录术后有无ER(即术后12个月内肿瘤复发)。采用单因素及多因素logistic回归比较训练集有、无ER患者临床、CT表现及术前病理资料,筛选ER独立危险因素,构建CT-术前病理模型。基于训练集静脉期CT图像提取及筛选LAESCC影像组学特征并建立影像组学模型,以之联合独立危险因素建立联合模型。绘制受试者工作特征(ROC)曲线,计算曲线下面积(AUC),评估各模型诊断效能。结果334例中,168例ER、166例无ER;训练集有、无ER均为117例、验证集51例ER、49例无ER。CT显示LAESCC长度、cT分期、cN分期及术前病理分化程度均为ER独立危险因素(P均<0.05);CT-术前病理模型在训练集和验证集的AUC分别为0.759和0.783。共选出10个最佳影像组学特征,以之建立的影像组学模型在训练集和验证集的AUC分别为0.770和0.730,联合模型在训练集和验证集的AUC分别为0.838和0.826。联合模型在训练集的AUC高于术前CT-病理模型及影像组学模型(P均<0.01)。结论CT影像组学联合CT及术前病理特征能有效预测LAESCC术后ER。

关键词：食管肿瘤癌鳞状细胞体层摄影 X线计算机影像组学肿瘤复发局部

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Plant multiscale networks:charting plant connectivity by multi-level analysis and imaging techniques

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Science China(Life Sciences) 2021年第9期64卷 1392-1422页

作者： Xi Zhang Yi Man Xiaohong Zhuang Jinbo Shen Yi Zhang Yaning Cui Meng Yu jingjing xing Guangchao Wang Na Lian Zijian Hu Lingyu Ma Weiwei Shen Shunyao Yang Huimin Xu Jiahui Bian Yanping Jing Xiaojuan Li Ruili Li Tonglin Mao Yuling Jiao Sodmergen Haiyun Ren Jinxing Lin Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing Forestry UniversityBeijing 100083China College of Biological Sciences and Biotechnology Beijing Forestry UniversityBeijing 100083China School of Life Sciences Centre for Cell&Developmental Biology and State Key Laboratory of AgrobiotechnologyThe Chinese University of Hong KongHong Kong 999077China State Key Laboratory of Subtropical Silviculture Zhejiang A&F UniversityHangzhou 311300China Key Laboratory of Cell Proliferation and Regulation Biology Ministry of EducationCollege of Life ScienceBeijing Normal UniversityBeijing 100875China Key Laboratory of Plant Stress Biology School of Life SciencesHenan UniversityKaifeng 457004China College of Biological Sciences China Agricultural UniversityBeijing 100193China State Key Laboratory of Plant Physiology and Biochemistry Department of Plant SciencesCollege of Biological SciencesChina Agricultural UniversityBeijing 100193China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyChinese Academy of Sciencesand National Center for Plant Gene ResearchBeijing 100101China Key Laboratory of Ministry of Education for Cell Proliferation and Differentiation College of Life SciencesPeking UniversityBeijing 100871China

In multicellular and even single-celled organisms,individual components are interconnected at multiscale levels to produce enormously complex biological networks that help these systems maintain homeostasis for development and environmental adaptation.Systems biology studies initially adopted network analysis to explore how relationships between individual components give rise to complex biological processes.Network analysis has been applied to dissect the complex connectivity of mammalian brains across different scales in time and space in The Human Brain Project.In plant science,network analysis has similarly been applied to study the connectivity of plant components at the molecular,subcellular,cellular,organic,and organism levels.Analysis of these multiscale networks contributes to our understanding of how genotype determines phenotype.In this review,we summarized the theoretical framework of plant multiscale networks and introduced studies investigating plant networks by various experimental and computational modalities.We next discussed the currently available analytic methodologies and multi-level imaging techniques used to map multiscale networks in plants.Finally,we highlighted some of the technical challenges and key questions remaining to be addressed in this emerging field.

关键词： multiscale network connectivity cytoskeleton membrane contact site organelle interaction multicellularity connectome cytoarchitecture topological analysis multi-level imaging techniques

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PIEZOELECTRIC PROPERTIES OF SINGLE-STRAND DNA MOLECULAR BRUSH BIOLAYERS

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Acta Mechanica Solida Sinica 2007年第3期20卷 206-210页

作者： Zhang Nenghui Shah Jinying xing jingjing Shanghai Institute of Applied Mathematics and Mechanics Shanghai University Shanghai 200072 China Department of Mechanics College of Sciences Shanghai University Shanghai 200444 China

The paper is devoted to investigations on nanomechanical behaviors of biochips in label-free biodetections. The chip consists of Si-layer, Ti-layer, Au-layer and single-strand DNA （ssDNA） molecular brush biolayer immobilized by self-assembly technology of thiol group. Unlike previous viewpoints, such as force-bending, entropy-bending and curvature electricity effect, etc., the piezoelectric effect of the biopolymer brush layer is viewed as the main factor that induces nanomechanical bending of biochips, and a classical macroscopic piezoelectric constitutive relation is used to describe the piezoelectric effect. A new laminated cantilever beam model with a piezoelectric biolayer in continuum mechanics, the linearized Poisson-Boltzmann equation in statistical mechanics and the scaling method in polyelectrolyte brush theory are combined to es- tablish a relationship between the nanomechanical deflection of DNA chips and the factors such as nanoscopic structural features of ssDNA molecules, buffer salt concentration, macroscopic mechanical/piezoelectric parameters of DNA chips etc. Curve fitting of experimental data shows that the sign of the piezoelectric constant of the biolayer may control the deflection direction of DNA chips during the packaging process.

关键词： DNA chip laminated beam polymer brush piezoelectric effect

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孕妇产前抑郁患病状况及其对小于胎龄儿的影响

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中国儿童保健杂志 2023年第8期31卷 895-898页

作者：邢京京北京市大兴区人民医院妇产科北京102600

目的分析孕妇产前抑郁患病情况及其对小于胎龄(SGA)儿发生的影响,为母婴预后良好提供依据。方法纳入2017年6月—2019年6月大兴区人民医院收治的537例孕妇,应用爱丁堡产后抑郁量表(EPDS)评价抑郁症状,根据是否合并抑郁分为抑郁组和对照组... 详细信息

目的分析孕妇产前抑郁患病情况及其对小于胎龄(SGA)儿发生的影响,为母婴预后良好提供依据。方法纳入2017年6月—2019年6月大兴区人民医院收治的537例孕妇,应用爱丁堡产后抑郁量表(EPDS)评价抑郁症状,根据是否合并抑郁分为抑郁组和对照组,比较两组孕妇的一般资料、分娩结局和新生儿体质量、头围、胸围及身长情况,确定抑郁对SGA儿的影响。结果抑郁组孕妇106例,产前患病率为19.74%。抑郁组的EPDS评分显著高于对照组(t=24.234,P<0.001),年龄显著小于对照组(t=2.480,P=0.013)。抑郁组的新生儿出生胎龄显著小于对照组(t=2.398,P=0.017),新生儿体质量、头围、胸围、身长显著低于对照组(t=4.862、4.775、9.301、10.758,P<0.001),新生儿早产比例和SGA比例显著高于对照组(χ^(2)=4.758、6.803,P<0.05)。Logistic回归分析显示抑郁(OR=1.802,95%CI:1.232~4.793,P=0.016)是SGA发生的独立危险因素。接受者操作特征曲线(ROC曲线)分析显示EPDS评分以12为界值预测SGA的曲线下面积为0.757。结论孕妇产前抑郁高发,影响胎儿发育,是SGA发生的独立危险因素。应早期对孕妇进行产前抑郁的筛检,对于有抑郁症状的孕妇,应及时进行心理疏导和干预,促进母婴健康。

关键词：孕妇产前抑郁小于胎龄儿

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Effect of drying methods on perovskite films and solar cells

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Journal of Semiconductors 2024年第1期45卷 1-5页

作者： Ling Liu Chuantian Zuo Guang-xing Liang Hua Dong jingjing Chang Liming Ding Center for Excellence in Nanoscience(CAS) Key Laboratory of Nanosystem and Hierarchical Fabrication(CAS)National Center for Nanoscience and TechnologyBeijing 100190China Key Laboratory of Semiconductor Materials Science Beijing Key Laboratory of Low Dimensional Semiconductor Materials and DeviceIsnstitute of SemiconductorsChinese Academy of SciencesBeijing 100083China College of Physics and Optoelectronic Engineering Shenzhen UniversityShenzhen 518060China School of Electronic and Information Engineering Xi’an Jiaotong UniversityXi’an 710049China Academy of Advanced Interdisciplinary Research Xidian UniversityXi’an 710126China

The high efficiency,solution processibility,and flexibility of perovskite solar cells make them promising candidates for the photovoltaic industry[1−8].The deposition method is one of the most critical factors that affect the performance of perovskite films.Various deposition methods have been developed to make perovskite films,including spin-coating,slotdie coating.

关键词： perovskite films critical

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Thermal Optimization of High-power Vertical Cavity Surface Emitting Laser Arrays

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Faguang Xuebao/Chinese Journal of Luminescence 2024年第6期45卷 970-977页

作者： Xia, Yuqi Mu, Jingfei Zhou, Yinli Zhang, xing Zhang, Jianwei Chen, Chao Yuan, Gaohui Zhang, Zhuo Liu, Tianjiao Bai, Haopeng Xu, Yuehui Sun, jingjing Ning, Yongqiang Wang, Lijun State Key Laboratory of Luminescence and Applications Changchun Institute of Optics Fine Mechanics and Physics Chinese Academy of Sciences Changchun 130033 China University of Chinese Academy of Sciences Beijing 100049 China

n this study，we designed a new parameter，the temperature impact factor，which considers the spacing between vertical-cavity surface-emitting laser（VCSEL）array units，size of the oxidation aperture，number of units，and the input current，and can be used to characterize the degree of thermal crosstalk in the array. Based on this，we designed an optimization layout algorithm for the VCSEL array and established a thermal-electric coupling model to verify the benefits of the optimized layout on temperature characteristics. Compared with the conventional layout，the temperature rise was reduced by 5%. In addition，under the condition of fixed current density and luminous area，reduction of the oxidation aperture and increasing the number of units can effectively improve the temperature characteristics of the VCSEL array. The average temperature of the 10 μm oxidation aperture was 30 K lower than that of the 30 μm oxidation aperture. The research results show that the impact of thermal crosstalk in the VCSEL array is effectively reduced and provide guidance for the design of the VCSEL array. © 2024 Editorial Office of Chinese Optics. All rights reserved.

关键词： Oxidation

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