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Elite sd1 alleles in japonica rice and their breeding applications in northeast China

The Crop Journal 2022年第1期10卷 224-233页

作者： Hanjing Sha Hualong Liu Guangxin Zhao Zhongmin Han Huilin Chang jingguo wang Hongliang Zheng Jifeng Zhang Yang Yu Yuqiang Liu Detang Zou Shoujun Nie Jun Fang Key Laboratory of Soybean Molecular Design Breeding Northeast Institute of Geography and AgroecologyChinese Academy of SciencesHarbin 150081HeilongjiangChina Key Laboratory of Germplasm Enhancement Physiology and Ecology of Food Crops in Cold RegionMinistry of EducationNortheast Agricultural UniversityHarbin 150030HeilongjiangChina Innovation Center Suihua Branch of Heilongjiang Academy of Agricultural SciencesSuihua 152052HeilongjiangChina University of Chinese Academy of Sciences Beijing 100049China College of Life Science and Engineering Shenyang UniversityShenyang 110044LiaoningChina The Innovative Academy for Seed Design Chinese Academy of SciencesBeijing 100101China

The Green Revolution gene sd1 has been used extensively in modern rice breeding,especially in indica cultivars.However,elite sd1 alleles and related germplasm resources used for japonica rice breeding have not been identified,and extensive efforts are needed for japonica rice breeding to obtain new dwarfing sources.Data from MBKbase-Rice revealed seven sd1 haplotypes in indica and four in japonica rice.Two new sd1 alleles were identified in indica rice.In 295 japonica accessions from northeast Asia,except for the weak functional allele SD1-EQ,sd1-r was the major allele,reducing plant height in comparison with SD1-EQ.Japonica germplasm resources carrying reported sd1 alleles were identified by genotype searching and further verified by literature search,genealogical analysis,and d Caps markers.Pedigrees and geographic distribution showed that sd1-r is an excellent allele widely used in northern China and Tohoku in Japan,and sd1-j is commonly used in east China and Kyushu in Japan.Dongnong-and Xiushui-series cultivars carrying sd1-r and sd1-j,respectively,are essential branches of the backbone parents of Chinese japonica rice,Akihikari and Ce21,with the largest number of descendants and derived generations.In semi-dwarf japonica rice breeding,sd1-d was introgressed into Daohuaxiang 2(DHX2).Dwarf and semi-dwarf lines carrying sd1-d were selected and designated as 1279 and 1280,respectively,after withstanding typhoon-induced strong winds and heavy rains in 2020,and are anticipated to become useful intermediate materials for future genetic research and breeding.This work will facilitate the introduction,parental selection,and marker-assisted breeding,and provide a material basis for the next step in identifying favorable genes that selected together with the sd1 alleles in japonica backbone parents.

关键词： Semi-dwarf breeding Elite allele Pedigree analysis Geographic distribution Regional adaptability

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Cryptobiosis-inspired assembly of “AND” logic gate platform for potential tumor-specific drug delivery

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Acta Pharmaceutica Sinica B 2021年第2期11卷 534-543页

作者： Hu Zhou Gang He Yanbin Sun jingguo wang Haitao Wu Ping Jin Zhengbao Zha Shenzhen Maternity and Child Healthcare Hospital Shandong UniversityShenzhen 518028China School of Food and Biological Engineering School of Instrument Science and Opto-Electronics EngineeringHefei University of TechnologyHefei 230009China

Developing tumor-specific drug delivery systems with minimized off-target cargo leakage remains an enduring challenge.In this study,inspired from the natural cryptobiosis explored by certain organisms and stimuli-responsive polyphenol-metal coordination chemistry,doxorubicin(DOX)-conjugated gelatin nanoparticles with protective shells formed by complex of tannic acid and FeⅢ(DG@TA-FeⅢNPs)were successfully developed as an"AND"logic gate platform for tumor-targeted DOX delivery.Moreover,benefiting from the well-reported photothermal conversion ability of TA-FeⅢcomplex,a synergistic tumor inhibition effect was confirmed by treating 4 T1 tumor-beaing mice with DG@TA-FeⅢNPs and localized near-infrared(NIR)laser irradiation.As a proof of concept study,this work present a simple strategy for developing"AND"logic gate platforms by coating enzyme-degradable drug conjugates with detachable polyphenol-metal shells.

关键词： Cryptobiosis "AND"logic gate Polyphenol-metal chemistry Drug delivery Tumor microenvironment

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Enhanced N2O emission rate in field soil undergoing conventional intensive fertilization is attributed to the shifts of denitrifying guilds

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Pedosphere 2021年第1期31卷 145-156页

作者： Mengmeng JI Hao TIAN Xiaoging WU Ji LI Ying ZHU Guojun WU Ting XU jingguo wang Xiaojun ZHANG Siate Key Laberaory of Micnbrial Metabolisom and School of Life Scriences and Bioechmology.Shanghai Jiav Tong CUmiesiy Shonghal 200240China College of Resoures and Envirommental Sciences China Agricwlural CniversilyBeijing 100193China

The emission of greenhouse gas N2O in agricultural soil is modulated by N fertilization that could be converted to N2O by denitrifiers under anaerobic condition. Nevertheless, the effect of denitrifiers on N2O emission has not been thoroughly elucidated. In this study, we explored the denitrifying gas kinetics,nitrate content, transcribed denitrifying functional genes(narG, nirS, nirK, and nosZ), and the active bacteria during anaerobic incubation of soils with conventional intensive N fertilization(CNS) and reduced N fertilization(RNS), both sampled from a vegetable greenhouse experimental site. The CNS sample showed significantly higher N2O emission rates relative to the RNS sample. However, the difference in N2O emission between the soils was neither because of the cumulative nitrate content nor the quantity of denitrifying gene transcripts. The distinct fertilization regimes shaped the significantly different bacterial communities in these soils. The absolute abundance of bacteria that produce N2O but lack the nosZ gene for N2O reduction(for example, the dominant Kaistobacter) was higher in CNS than in RNS. Meanwhile, the abundance of two operational taxonomy units(OTUs), namely Rhodanobacter,belonging to the most abundant genus in denitrifying guilds, was strongly enriched in CNS and showed significant positive correlation with N2O/(N2O +N2). The predominance of these bacterial OTUs in the CNS denitrifying guild strongly suggested that high N2O emission from the soil with long-term conventional intensive fertilization might be primarily attributed to the reshaping of distinct denitrifying guilds in their bacterial communities.

关键词： denitrification denitrifier community denitrifying bacteria denitrifying functional gene greenhouse gas N fertilization

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Phase Relations in TiAl+Nb System

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Journal of Materials Science & Technology 1994年第5期10卷 359-366页

作者： jingguo wang Guoliang CHEN and Zuqing SUN (State Key Lab. for Advanced Metal Materials, University of Science and Technology Beijing, Beijing, 100083, China)Hengqiang YE (Lab. of Atomic Imaging of Solids, Institute of Metal Research, Academia Sinica, Sh Univ of Science and Technology Beijing China

The microstructures and phases of ternary TiAl+Nb alloys containing 50-60 at.-%Al, 0-21 at.-%Nb have been studied using transmission electron microscopy (TEM) and X-ray powder diffraction. The phases present in the alloys and their distribution were found to be a sensitive function of composition. The phase relations between γ-TiAl and γ1 (a new ordered ternary intermetallic compound based on γ-TiAl) were determined. Essentially single γ phase was determined for alloys with relativety low Nb content (≤10 at.-%Nb). the γ1 phase was determined to exist in the composition range containing higher Nb contents (15-21 at.-%Nb). Between γ and γ1 phases, with intermediate Nb contents there is a transitional phase γ1 (a superstructure of γ-TiAl). As for the influence of Al concentration on the phase relations. the γ1 phase was inclined to form in the alloys with relatively high Al contents. The ordering transformation of γ, to γ1 is a continuous ordering process and the transition may be second order.

关键词： TiAl nm PA Phase Relations in TiAl+Nb System Chen

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阿尔金地区锂铍稀有金属找矿进展及成矿远景分析

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新疆地质 2024年第2期42卷 224-229页

作者：华克强王敬国张朋丁海波新疆维吾尔自治区地质矿产勘查开发局第三地质大队新疆库尔勒841000

锂铍稀有金属是推动现代工业革命和高新技术发展的重要物质基础,其安全的供应关系到我国战略性新兴产业的发展。2014年新疆地矿局第三地质大队在阿尔金地区首次发现吐格曼铍矿,发现评价了瓦石峡南、阿亚克、库木萨依、塔什达坂等4个大-... 详细信息

锂铍稀有金属是推动现代工业革命和高新技术发展的重要物质基础,其安全的供应关系到我国战略性新兴产业的发展。2014年新疆地矿局第三地质大队在阿尔金地区首次发现吐格曼铍矿,发现评价了瓦石峡南、阿亚克、库木萨依、塔什达坂等4个大-超大型锂铍稀有金属矿田,实现了我国锂资源在新区域的重大找矿突破。厘定长300 km,宽10~35 km的阿尔金锂铍稀有金属成矿带,初步估算潜在氧化锂资源量300×10^(4)t,有望成为一个国家级锂铍大型资源基地。在以往锂铍稀有金属矿勘查、研究的基础上,揭示该地区伟晶岩型锂铍稀有金属成矿远景,为下一步找矿和勘查部署提供有力支撑。

关键词：阿尔金地区锂铍稀有金属矿找矿进展远景分析

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OsADR3 increases drought stress tolerance by inducing antioxidant defense mechanisms and regulating OsGPX1 in rice(Oryza sativa L.)

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The Crop Journal 2021年第5期9卷 1003-1017页

作者： Jiaming Li Minghui Zhang Luomiao Yang Xinrui Mao Jinjie Li Lu Li jingguo wang Hualong Liu Hongliang Zheng Zichao Li Hongwei Zhao Xianwei Li Lei Lei Jian Sun Detang Zou Key Laboratory of Germplasm Enhancement Physiology and Ecology of Food Crops in Cold RegionMinistry of EducationNortheast Agricultural UniversityHarbin 150030HeilongjiangChina College of Life Science Northeast Agricultural UniversityHarbin 150030HeilongjiangChina Key Laboratory of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Lab of Crop Genetic Improvement China Agricultural UniversityBeijing 100094China

The C (Cys) 2H (His) 2-type transcription factor is one of the most important transcription factors in plants and plays a regulatory role in the physiological responses of rice to abiotic stresses.A novel rice C2H2-type zinc finger protein,abscisic acid (ABA)-drought-reactive oxygen species (ROS) 3 (OsADR3),was found to confer drought stress tolerance by enhancing antioxidant defense and regulating Os GPX1.Overexpression of OsADR3 in rice increased tolerance to drought stress by increasing ROS scavenging ability and ABA sensitivity.In contrast,CRISPR/Cas9-mediated knockout of osadr3 increased the sensitivity of rice to drought and oxidative stress.An exogenous ROS-scavenging reagent restored the droughtstress tolerance of osadr3-CRISPR plants.Global transcriptome analysis suggested that OsADR3 increased the expression of Os GPX1 under drought stress.Electrophoretic mobility shift,yeast one-hybrid,and dualluciferase reporter assays revealed that OsADR3 modified the expression of Os GPX1 by directly binding to its promoter.Knockdown of Os GPX1 repressed ROS scavenging ability under drought stress in OsADR3-overexpression plants.These findings suggest that OsADR3 plays a positive regulatory role in droughtstress tolerance by inducing antioxidant defense and associated with the ABA signaling pathway in rice.

关键词： Drought stress Rice ROS scavenging Glutathione peroxidase Abscisic acid

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若羌塔什达坂锂矿床矿物分带特征及找矿意义

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新疆地质 2024年第2期42卷 269-274页

作者：张朋王敬国夏昭德黄俊凯罗新涛华克强新疆维吾尔自治区地质矿产勘查开发局第三地质大队新疆库尔勒841000 长安大学地球科学与资源学院陕西西安710054

塔什达坂锂矿床位于阿尔金稀有金属成矿带的东段,锂矿体赋存于花岗质伟晶岩中,已圈定23条含锂花岗伟晶岩脉,含锂矿物主要为锂辉石、锂云母和锂电气石,伟晶岩脉主要为含锂电气石伟晶岩、锂辉石伟晶岩、锂云母伟晶岩及不含矿黑色电气石伟... 详细信息

塔什达坂锂矿床位于阿尔金稀有金属成矿带的东段,锂矿体赋存于花岗质伟晶岩中,已圈定23条含锂花岗伟晶岩脉,含锂矿物主要为锂辉石、锂云母和锂电气石,伟晶岩脉主要为含锂电气石伟晶岩、锂辉石伟晶岩、锂云母伟晶岩及不含矿黑色电气石伟晶岩,矿物分带明显,其中ρ52号脉特征矿物变化最典型。塔什达坂锂矿床一区南部ρ52~ρ59、ρ58~ρ70之间的区域存在隐伏矿体,通过矿床地质特征及矿物分带研究,为区域锂矿的勘查评价提供找矿方向。

关键词：矿床地质花岗伟晶岩矿物分带含锂矿物塔什达坂锂矿

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多连杆刚柔机械手设计与手指减振分析

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西安工程大学学报 2024年第2期38卷 116-123页

作者：管声启郝振虎王静国马腾飞师红宇西安工程大学机电工程学院陕西西安710048 西安工程大学计算机科学学院陕西西安710048

针对陶瓷易碎零件分拣过程中难以稳定抓取的问题,设计了一种能够有效稳定抓取的新型多连杆刚柔机械手。首先,以机械传动为基础,将刚性关节与柔性材料相结合,设计机械手的传动方式与柔顺关节的最优结构,并在三维软件上构建刚柔机械手的... 详细信息

针对陶瓷易碎零件分拣过程中难以稳定抓取的问题,设计了一种能够有效稳定抓取的新型多连杆刚柔机械手。首先,以机械传动为基础,将刚性关节与柔性材料相结合,设计机械手的传动方式与柔顺关节的最优结构,并在三维软件上构建刚柔机械手的整体结构;其次,通过对机械手手指结构进行简化,采用拉格朗日理论和有限元离散方法,构建出具有尖端质量的手指多连杆刚柔耦合动力学模型;最后,求解出机械手手指在运动过程中的动力学方程。在此基础上,通过设计减振方案,对机械手手指结构做出优化和改进。仿真实验结果表明:在外激力为2 N的条件下,改进结构后的刚柔机械手手指其振动的幅度降低8 mm,且恢复稳定的时间缩短了4 s以上,在一定程度上提高了分拣和抓取过程中的抗冲击力,保证了对陶瓷易碎零件抓取过程的稳定性。

关键词：零件分拣结构设计有限元分析刚柔机械手减振实验

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阿尔金锂矿带的发现厘定及战略意义

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新疆地质 2024年第2期42卷 284-288页

作者：王敬国丁海波张朋杨智全华克强何海军新疆维吾尔自治区地质矿产勘查开发局第三地质大队新疆库尔勒841000

伟晶岩型锂矿是世界锂矿床中仅次于盐湖卤水型锂矿的一种重要硬岩型锂矿,具品位高、易开采、提锂技术成熟的特点,在我国锂资源勘查开发中占有非常重要的地位。自2014年阿尔金山地区首次发现吐格曼铍矿点以来,新厘定长230 km,宽10~35 km... 详细信息

伟晶岩型锂矿是世界锂矿床中仅次于盐湖卤水型锂矿的一种重要硬岩型锂矿,具品位高、易开采、提锂技术成熟的特点,在我国锂资源勘查开发中占有非常重要的地位。自2014年阿尔金山地区首次发现吐格曼铍矿点以来,新厘定长230 km,宽10~35 km的锂矿带,发现我国首例古生代大型伟晶岩锂矿床,确定了伟晶岩+热液复合型锂铍稀有金属矿床的新类型,实现了我国锂资源在新区域、新层系的重大找矿突破。2023年稀长沟矿区钻探验证取得单孔累计见矿视厚96 m的再次重大突破,有力支撑了自治区建设“若羌新城”重要决策,助力南疆构建以新能源产业链为支撑的现代能源经济体系,勘查开发战略意义重大。

关键词：阿尔金锂矿带发现厘定伟晶岩型战略意义

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Three-channel CMOS transimpedance amplifier for LiDAR sensor receiver

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Journal of Systems Engineering and Electronics 2024年第1期35卷 74-80页

作者： LIU Ruqing ZHU jingguo JIANG Yan LI Feng JIANG Chenghao MENG Zhe Photoelectric R&D Center Academy of Opto-ElectronicsChinese Academy of SciencesBeijing 100094China

For time-of-flight(TOF)light detection and ranging(LiDAR),a three-channel high-performance transimpedance amplifier(TIA)with high immunity to input load capacitance is presented.A regulated cascade(RGC)as the input stage is at the core of the complementary metal oxide semiconductor(CMOS)circuit chip,giving it more immunity to input photodiode detectors.A simple smart output interface acting as a feedback structure,which is rarely found in other designs,reduces the chip size and power consumption simultaneously.The circuit is designed using a 0.5μm CMOS process technology to achieve low cost.The device delivers a 33.87 dB?transimpedance gain at 350 MHz.With a higher input load capacitance,it shows a-3 dB bandwidth of 461 MHz,indicating a better detector tolerance at the front end of the system.Under a 3.3 V supply voltage,the device consumes 5.2 mW,and the total chip area with three channels is 402.8×597.0μm2(including the test pads).

关键词： transimpedance amplifier(TIA) three-channel regulated cascade(RGC) light detection and ranging(LiDAR)

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