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Real-time monitoring flexible hydrogels based on dual physically cross-linked network for promoting wound healing

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中国化学快报:英文版 2023年第10期34卷 229-235页

作者： Le Hu Yuxin Wang Qing Liu Man Liu Faming Yang Chunxiao Wang Panpan Pan Lin Wang Li chen Jingdi Chen Marine College Shandong UniversityWeihai 264209China Shandong Laboratory of Advanced Materials and Green Manufacturing Yantai 265599China College of Biological Science and Technology Fuzhou UniversityFuzhou 350108China

To achieve smart and personalized medicine, the development of hydrogel dressings with sensing properties and biotherapeutic properties that can act as a sensor to monitor of human health in real-time while speeding up wound healing face great challenge. In the present study, a biocompatible dual-network composite hydrogel(DNCGel) sensor was obtained via a simple process. The dual network hydrogel is constructed by the interpenetration of a flexible network formed of poly(vinyl alcohol)(PVA) physical cross-linked by repeated freeze-thawing and a rigid network of iron-chelated xanthan gum(XG) impregnated with Fe^(3+) interpenetration. The pure PVA/XG hydrogels were chelated with ferric ions by immersion to improve the gel strength(compressive modulus and tensile modulus can reach up to 0.62 MPa and0.079 MPa, respectively), conductivity(conductivity values ranging from 9 × 10^(-4) S/cm to 1 × 10^(-3)S/cm)and bacterial inhibition properties(up to 98.56%). Subsequently, the effects of the ratio of PVA and XG and the immersion time of Fe^(3+) on the hydrogels were investigated, and DNGel3 was given the most priority on a comprehensive consideration. It was demonstrated that the DNCGel exhibit good biocompatibility in vitro, effectively facilitate wound healing in vivo(up to 97.8% healing rate) under electrical stimulation, and monitors human movement in real time. This work provides a novel avenue to explore multifunctional intelligent hydrogels that hold great promise in biomedical fields such as smart wound dressings and flexible wearable sensors.

关键词： Conductive hydrogel Dual cross-linked network Antimicrobial activity Real-time monitorin Wound healing

A fast on-demand preparation of injectable self-healing nanocomposite hydrogels for efficient osteoinduction

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Chinese Chemical Letters 2021年第7期32卷 2159-2163页

作者： Panpan Pan Xiao chen Huaran Xing Yonghui Deng Jingdi Chen Fahad AAlharthi Abdulaziz AAlghamdi Jiacan Su Department of Chemistry Department of GastroenterologyZhongshan Hospital of Fudan UniversityState Key Laboratory of Molecular Engineering of PolymersFudan UniversityShanghai 200433China Department of Orthopaedics Trauma Changhai HospitalSecond Military Medical UniversityShanghai 200433China Institute of Biomedical and Pharmaceutical Technology Fuzhou UniversityFuzhou 350002China Marine College Shandong UniversityWeihai 264209China Department of Chemistry College of ScienceKing Saud UniversityP.O.Box 2455Riyadh 11451Saudi Arabia State Key Lab of Transducer Technology Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050China

Injectable hydrogels have been considered as promising materials for bone regeneration,but their osteoinduction and mechanical performance are yet to be improved.In this study,a novel biocompatible injectable and self-healing nano hybrid hydrogel was on-demand prepared via a fast(within 30 s)and easy gelation approach by reversible Schiff base formed between-CH=O of oxidized sodium alginate(OSA)and-NH2 of glycol chitosan(GCS)mixed with calcium phosphate nanoparticles(CaP NPs).Its raw materials can be ready in large quantities by a simple synthesis process.The mechanical strength,degradation and swelling behavior of the hydrogel can be readily controlled by simply controlling the molar ratio of-CH=O and-NH2.This hydrogel exhibits pH responsiveness,good degradability and biocompatibility.The hydrogel used as the matrix for mesenchymal stem cells can significantly induce the proliferation,differentiation and osteoinduction in vitro.These results showed this novel hydrogel is an ideal candidate for applications in bone tissue regeneration and drug delivery.

关键词： Dynamic coordination Nanocomposite hydrogels Fast and easy on-demand preparation Injectable and self-healing abilities Stem cell osteoinduction

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防根面龋牙科材料的研究进展

口腔疾病防治 2024年第1期32卷 64-69页

作者：熊净迪程兴群吴红崑口腔疾病防治全国重点实验室国家口腔医学中心国家口腔疾病临床医学研究中心四川大学华西口腔医院老年口腔科四川成都610041

根面龋是老年人口腔常见病和多发病,发病率和未治疗率高,治疗难度大,可迅速进展引起牙髓根尖周病或残冠根,是老年人失牙的重要原因,已成为严重影响老年人生活质量的常见口腔慢性病之一。因此,根面龋早期干预和防治非常重要。传统防根面... 详细信息

根面龋是老年人口腔常见病和多发病,发病率和未治疗率高,治疗难度大,可迅速进展引起牙髓根尖周病或残冠根,是老年人失牙的重要原因,已成为严重影响老年人生活质量的常见口腔慢性病之一。因此,根面龋早期干预和防治非常重要。传统防根面龋牙科材料如氟化物、氯己定等在临床中广泛应用,但是其具有牙齿着色、再矿化及杀菌效率低下等缺点。近年来,一系列新兴防根面龋牙科材料如无机纳米材料、胶原交联剂等逐步成为研究热点,新兴材料具有促进深部牙体组织再矿化、作用时间延长、黏附力增强等优点。未来防龋材料应根据根面龋的特点及应用人群进行设计,朝着简便化、高效化、低毒性方面发展。本文对目前防根面龋牙科材料的研究及应用进行综述,以期能够为防根面龋牙科材料的研究提供理论基础,对促进根面龋的有效预防,改善老年人的口腔健康状况和生活质量具有重要意义。

关键词：根面龋防龋材料再矿化口腔保健人口老龄化龋病预防抗菌材料氟化物

The conformational dynamics of Cas9 governing DNA cleavage are revealed by single-molecule FRET

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The conformational dynamics of Cas9 governing DNA cleavage a...

Unconventional ferroelectricity in half-filling states of antiparallel stacking of twisted WSe_(2)

中国化学会第五届全国生物物理化学会议（NCBPC5）

作者：杨梦铱 Sijia Peng Ruirui Sun jingdi Lin Nan Wang Chunlai chen School of Life Sciences Tsinghua-Peking Joint Center for Life SciencesBeijing Advanced Innovation Center for Structural BiologyTsinghua University

＜正＞Off-target binding and cleavage by Cas9 pose major challenges in its application.How the conformational dynamics of Cas9 govern its nuclease activity under on-and off-target conditions remains largely unknown.Here,using intra-molecular single-molecule fluorescence resonance energy transfer measurements,we revealed that Cas9 in apo,sgRNA-bound,and dsDNA/sgRNA-bound forms spontaneously transits among three major conformational states,mainly reflecting significant conformational mobility of the catalytic HNH domain.

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National Science Open 2023年第1期2卷 24-34页

作者： Liheng An Zishu Zhou Xuemeng Feng Meizhen Huang Xiangbin Cai Yong chen Pei Zhao Xi Dai jingdi Zhang Wang Yao Junwei Liu Ning Wang Department of Physics and Center for Quantum Materials the Hong Kong University of Science and TechnologyHong KongChina William Mong Institute of Nano Science and Technology the Hong Kong University of Science and TechnologyHong KongChina Department of Physics University of Hong KongHong KongChina

We report on emergence of an abnormal electronic polarization in twisted double bilayer WSe_(2) in antiparallel interface stacking geometry,where local centrosymmetry of atomic registries at the twist interface does not favor the spontaneous electronic polarizations as recently observed in the parallel interface stacking geometry.The unconventional ferroelectric behaviors probed by electronic transport measurement occur at half filling insulating states at 1.5 K and gradually disappear at about 40 K.Single band Hubbard model based on the triangular moirélattice and the interlayer charge transfer controlled by insulating phase transition are proposed to interpret the formation of electronic polarization states near half filling in twisted WSe_(2) devices.Our work highlights the prominent role of many-body electronic interaction in fostering novel quantum states in moiré-structured systems.

关键词： two-dimensional semiconductor twist moiré ferroelectricity electron interaction electronic transport

踝关节保护支具对空降兵着陆时踝关节防护效果评价研究

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中华劳动卫生职业病杂志 2023年第7期41卷 504-508页

作者：黄新宇郁英杰王亦君刘涛陈敬地吴磊武汉科技大学公共卫生学院武汉430065 中国人民解放军空降兵医院骨科武汉430101 "职业危害识别与控制"湖北省重点实验室武汉430065

目的研究踝关节保护支具在模拟跳伞着陆阶段对踝关节的防护效果。方法于2021年8月,用单纯随机抽样法抽取30名男性空降兵为受试对象,先后在穿戴和未穿戴踝关节保护支具的情况下,分别从1.5 m、2.0 m高度的跳台跳下,以半蹲式跳伞着陆姿势... 详细信息

目的研究踝关节保护支具在模拟跳伞着陆阶段对踝关节的防护效果。方法于2021年8月,用单纯随机抽样法抽取30名男性空降兵为受试对象,先后在穿戴和未穿戴踝关节保护支具的情况下,分别从1.5 m、2.0 m高度的跳台跳下,以半蹲式跳伞着陆姿势着陆于沙地。试验分为1.5 m试验组和对照组与2.0 m试验组和对照组。应用角度传感器和表面肌电仪分别对踝关节冠状面倾斜范围和踝关节周围肌肉的最大随意收缩百分比(MVE%)进行测量和分析,评价踝关节保护支具对踝关节的防护效果。结果相同高度下,试验组受试对象踝关节冠状面倾斜范围均较对照组明显减小,差异均有统计学意义(P<0.05)。相同防护状态下,1.5 m组踝关节冠状面倾斜范围较2.0 m组明显减小,差异有统计学意义(P<0.05)。2 m试验组踝关节冠状面倾斜范围明显小于1.5 m对照组,差异有统计学意义(P<0.05)。与1.5 m对照组比较,1.5 m试验组右侧胫骨前肌和双侧外侧腓肠肌的MVE%减小,而双侧腓骨长肌的MVE%增大,差异均有统计学意义(P<0.05)。与2.0 m对照组比较,2.0 m试验组双侧胫骨前肌和右侧外侧腓肠肌的MVE%减小,双侧腓骨长肌的MVE%增大,差异均有统计学意义(P<0.05)。1.5 m试验组较2.0 m试验组双侧胫骨前肌、双侧外侧腓肠肌和右侧腓骨长肌的MVE%减小,差异均有统计学意义(P<0.05)。1.5 m对照组较2.0 m对照组双侧胫骨前肌、右侧外侧腓肠肌和右侧腓骨长肌的MVE%减小,差异均有统计学意义(P<0.05)。结论佩戴踝关节保护支具可以有效地限制踝关节冠状面倾斜范围,提高踝关节稳定性,减少着陆对踝关节周围肌肉的负荷;降低跳台高度能减小着陆时踝关节冠状面倾斜范围和踝关节周围肌肉负荷。

关键词：踝关节空降兵踝关节保护支具关节倾斜范围表面肌电图

A new bone repair scaffold combined with chitosan/ hydroxyapatite and sustained releasing icariin

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Chinese Science Bulletin 2009年第17期54卷 2953-2961页

作者： WU Tao NAN KaiHui chen jingdi JIN Dan JIANG Shan ZHAO PeiRan XU JunChang DU Hao ZHANG XiaoQiang LI JianWei PEI GuoXian Department of Orthopaedics and Traumatology Nanfang Hospital Guangzhou 510515 China The Affiliated Ophthalmology Hospital Wenzhou Medical College Wenzhou 325027 China Key Laboratory of Special Functional Materials Ministry of Education South China University of Technology Guangzhou 510640 China Department of Orthopaedics Xijing Hospital Xi'an 710032 China

Icariin, a plant-derived flavonol glycoside, has been proved as an osteoinductive agent for bone tissue engineering. A new bone repair scaffold was generated by thorough mixing of icariin and chitosan/ hydroxyapatite (icariin-CS/HA) using freeze-drying technigue. Characteristics of morphology, mechanical properties, biocompatibility, drug release behavior and bone repair abilities in vivo were evaluated. The results show that drug loading process of icariin did not affect physical structure of CS/HA composite significantly but decreased mechanical properies of CS/HA composite, which happened with a high dosage; icariin-CS/HA had favorable cell compatibility and promoted osteogenic differentiation of hBMSCs; the controlled release of icariin was satisfactory and the release retained after 90 d in vitro. In addition, icariin-CS/HA scaffolds had favorable osteoconduction and osteoinduction in vivo, and could fill bone defect sites and stimulate newborn bone tissues formation at early stage. On the basis of these data, icariin-CS/HA is believed to be an optical bone repair scaffold for tissue engineering.

关键词：组织工程支架淫羊藿苷羟基磷灰石骨修复壳聚糖缓释复合材料骨组织工程

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TRIM59在结直肠癌中的表达及意义

武汉大学学报（医学版） 2023年第7期44卷 814-818页

作者：伍威陈敬地黄凯林军邹莉萍武汉大学人民医院重症医学科湖北武汉430060 空降兵部队医院骨科湖北武汉430021 武汉大学中南医院消化内科湖北武汉430071 武汉大学人民医院消化内科湖北武汉430060 武汉大学人民医院教学办公室湖北武汉430060

目的:检测TRIM59在结直肠癌及癌旁正常组织中的表达,分析其表达变化与结直肠癌临床病理特征及预后的相关性,探讨其在结直肠癌中的作用。方法:采用实时荧光定量PCR和免疫印迹法检测8份结直肠癌组织及其癌旁组织中TRIM59 mRNA和蛋白的表... 详细信息

目的:检测TRIM59在结直肠癌及癌旁正常组织中的表达,分析其表达变化与结直肠癌临床病理特征及预后的相关性,探讨其在结直肠癌中的作用。方法:采用实时荧光定量PCR和免疫印迹法检测8份结直肠癌组织及其癌旁组织中TRIM59 mRNA和蛋白的表达情况。通过免疫组织化学法检测30例结直肠癌组织及16例癌旁组织中TRIM59蛋白的表达情况,比较两种组织中TRIM59蛋白的表达差异。收集NCBI的基因表达汇编(GEO)中的编码基因表达谱数据集,对结直肠癌肿瘤样本表达谱资料及其对应的生物学信息进行相关性和预后分析。结果:结直肠癌组织中TRIM59 mRNA相对表达量为0.0956±0.0111,高于癌旁组织的0.0239±0.0162,差异有统计学意义(t=9.31,P=0.011)。免疫印迹结果表明TRIM59在癌组织中的表达高于癌旁组织。免疫组化结果表明,TRIM59在结直肠癌组织的阳性表达率为86.7%(26/30),高于癌旁正常组织的25%(4/16),差异有统计学意义(U=67,P<0.001)。在结直肠癌中,高表达TRIM59的样本与Dukes分期相关(H=13.067,P=0.004);TRIM59高表达的结直肠癌患者预后更差(P<0.05)。结论:TRIM59在结直肠癌组织中的表达较癌旁组织高,且与结直肠癌的分期和生存率均有关,TRIM59可能参与了结直肠癌的进展。

关键词：结直肠癌 TRIM59 分期预后

柚皮苷-壳聚糖/羟基磷灰石复合支架修复大鼠颅骨缺损

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中国组织工程研究 2022年第28期26卷 4441-4445页

作者：卢育南张信照林斌斌徐敢陈景帝陈顺有厦门大学附属福州第二医院小儿骨科福建省福州市350007 福州大学生物和医药技术研究院福建省福州市350002

背景:骨碎补的有效成分柚皮苷具有补肝肾强筋骨的传统功效,能增加骨痂厚度,提高骨折愈合质量。目的:探究载中药骨碎补有效成分柚皮苷壳聚糖/羟基磷灰石复合支架的骨传导和骨诱导性能。方法:将一定钙磷比的羟基磷灰石前体液与含柚皮苷的... 详细信息

背景:骨碎补的有效成分柚皮苷具有补肝肾强筋骨的传统功效,能增加骨痂厚度,提高骨折愈合质量。目的:探究载中药骨碎补有效成分柚皮苷壳聚糖/羟基磷灰石复合支架的骨传导和骨诱导性能。方法:将一定钙磷比的羟基磷灰石前体液与含柚皮苷的壳聚糖溶液在碱性条件下原位结晶、冷冻干燥,获得柚皮苷-壳聚糖/羟基磷灰石多孔支架。将15只成年SD大鼠随机分成空白组(n=5)、对照组(n=5)和实验组(n=5),建立直径5 mm颅骨骨缺损模型,空白组未填充生物材料,对照组填充壳聚糖/羟基磷灰石支架,实验组填充柚皮苷-壳聚糖/羟基磷灰石复合支架。术后4周取材,CT扫描观察颅骨修复情况,苏木精-伊红染色观察颅骨修复的形态学,骨形态发生蛋白2和血管内皮生长因子免疫组化染色后观察缺损区域局部成骨活性因子的表达。结果与结论:①CT扫描显示,空白组大鼠颅骨未见明显骨生成,仅在缺损边缘可见少量新生骨;对照组于缺损孔隙可见新生骨形成,新生骨较少;实验组骨缺损修复良好,新生骨组织与缺损孔隙周围颅骨密度相似,大面积新生骨广泛填充了缺损孔隙。②苏木精-伊红染色显示,空白组缺损区填充以稀薄的疏松网状纤维组织,可见大量炎性反应病灶,仅在缺损边缘有少量新骨生成;对照组与实验组缺损区均可见支架材料残留,新生骨小梁及成骨细胞成簇分布于支架孔隙及缺损区边缘,周围伴有大量的毛细血管,其中以实验组表现出更强的新骨爬行替代能力。③免疫组化染色显示,实验组局部成骨活性因子骨形态发生蛋白2和血管内皮生长因子的表达高于对照组、空白组(P<0.05)。④结果表明,柚皮苷-壳聚糖/羟基磷灰石复合支架可为骨缺损修复提供必要的载体,柚皮苷可创造局部成骨微环境、加速新生骨组织的生长,具有良好的骨修复性能。

关键词：柚皮苷壳聚糖羟基磷灰石复合支架骨缺损成骨性能