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Germline promoter hypermethylation of tumor suppressor genes in gastric cancer

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World Journal of Gastroenterology 2012年第1期18卷 70-78页

作者： pu-Yuan Wu Zheng Zhang jing-Mei Wang wen-wen Guo Nong Xiao Qiong He Ya-Ping Wang Yi-Mei Fan Department of Medical Genetics Medical SchoolNanjing University Nanjing 210093 Jiangsu Province China Jiangsu Key Laboratory of Molecular MedicineNanjing 210093 Jiangsu Province China Department of Pathology Drum Tower Hos-pital Affiliated to Medical School Nanjing University Nanjing210093 Jiangsu Province China Departmem of Pathology Lujiang Hos-pital Lujiang Chaohu 231500 Anhui Province China

AIM: To explore germline hypermethylation of the tumor suppressor genes MLH1, CDH1 and P16INK4a in suspected cases of hereditary gastric cancer (GC). METHODS: A group of 140 Chinese GC patients in whom the primary cancer had developed before the age of 60 or who had a familial history of cancer were screened for germline hypermethylation of the MLH1, CDH1 and P16INK4a tumor suppressor genes. GenomicDNA was extracted from peripheral blood leukocytes and modified by sodium bisulfite. The treated DNA was then subjected to bisulfi te DNA sequencing for a specif ic region of the MLH1 promoter. The methylation status of CDH1 or P16INK4a was assayed using methylation-specif ic PCR. Clonal bisulf ite allelic sequencing in positive samples was performed to obtain a comprehensive analysis of the CpG island methylation status of these promoter regions. RESULTS: Methylation of the MLH1 gene promoter was detected in the peripheral blood DNA of only 1/140 (0.7%) of the GC patient group. However, this methylation pattern was mosaic rather than the allelic pattern which has previously been reported for MLH1 in hereditary non-polyposis colorectal cancer (HNPCC) patients. We found that 10% of the MLH1 alleles in the peripheral blood DNA of this patient were methylated, consistent with 20% of cells having one methylated allele. No germline promoter methylation of the CDH1 or P16INK4a genes was detected. CONCLUSION: Mosaic germline epimutation of the MLH1 gene is present in suspected hereditary GC patients in China but at a very low level. Germline epimutation of the CDH1 or P16INK4a gene is not a frequent event.

关键词： Gastric cancer Germline promoter methylation MLH1 CDH1 P16INK4a

Synthesis and Crystal Structure of 2-Hydroxybenzoic Acid [1-(3,5-Dibromo-2-hydroxyphenyl)methylidene]hydrazide Methanol

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Chinese Journal of Structural Chemistry 2007年第5期26卷 547-550页

作者： WANG Nong LI jing-Ping pu Yan-Ling School of Chemical and Biological Engineering Lanzhou Jiaotong University Lanzhou 730070 China

A new Schiff base compound, 2-hydroxybenzoic acid [1-（3,5-dibromo-2-hydroxyphenyl） methylidene]hydrazide methanol （C14H10Br2N2O3·CH3OH）, has been synthesized by the condensation of equimolar 3,5-dibromosalicylaldehyde and 2-hydroxybenzoic acid hydrazide in a methanol solution. The compound was characterized by elemental analysis, IR spectra, and singlecrystal X-ray diffraction. The compound consists of a Schiff base moiety 2-hydroxybenzoic acid [1-（3,5-dibromo-2-hydroxyphenyl）methylidene]hydrazide and a lattice methanol molecule. The crystal belongs to the monoclinic system, space group P21/n with a = 7.183（1）, b = 15.673（2）, c = 15.001（2） A, β = 98.345（2）°, Z = 4, V = 1670.9（4） A^3, Dc = 1.773 g/cm^3, Mr = 446.10, λ（MoKα） = 0.71073 , μ = 4.872 mm^-1, F（000） = 880, R = 0.0458 and wR = 0.0963. A total of 3445 unique reflections were collected, of which 2236 with I 〉 2σ（Ⅰ） were observed. As expected, the molecule adopts a trans configuration about the C=N double bond. The two benzene rings are nearly coplanar （mean deviation from the combined plane is 0.061（4） ）, with the dihedral angle of 7.9（3）°. The preliminary biological tests show that the compound has moderate antibacterial activities.

关键词： Schiff base acylhydrazone crystal structure antibacterial activity

Ultrafine Pt nanoparticles supported on double-shelled C/TiO2 hollow spheres material as highly efficient methanol oxidation catalysts

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Journal of Energy Chemistry 2020年第10期29卷 275-282页

作者： Xiaoyu Yue Yuguang pu wen Zhang Ting Zhang Wei Gao Department of Chemical and Materials Engineering the University of AucklandPrivate Bag 92019Auckland 1142New Zealand

Catalyst support is extremely important for future fuel cell *** this work,we developed doubleshelled C/TiO2(DSCT)hollow spheres as an excellent catalyst support via a template-directed *** combination of hollow structure,TiO2 shell and carbon layer results in excellent electron conductivity,electrocatalytic activity,and chemical *** uniformed DSCT hollow spheres are used as catalyst support to synthesize Pt/DSCT hollow spheres *** resulting Pt/DSCT hollow spheres exhibited high catalytic performance with a current density of 462 mA mg^-1 for methanol oxidation reaction,which is 2.52 times higher than that of the commercial Pt/***,the increased tolerance to carbonaceous poisoning with a higher If/Ibratio and a better long-term stability in acid media suggests that the DSCT hollow sphere is a promising C/TiO2-based catalyst support for direct methanol fuel cells applications.

关键词： Catalyst support C/TiO2 hollow sphere Metal-support interactions Methanol oxidation reaction

Conjugated polyelectrolytes for label-free visual detection of heparin

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Science China Chemistry 2011年第4期54卷 567-574页

作者： pu KanYi ZHAN RuoYu LIANG jing LIU Bin Department of Chemical and Biomolecular Engineering National University of SingaporeSingapore 117576Singapore

Conjugated polyelectrolytes (CPEs),with electron-delocalized backbones and charged ionic side chains,are unique sensory materials for the construction of optical *** this feature article,CPE-based visual detection of heparin is *** CPE-based heparin probes,including ratiometric,light-up and colormetric probes,are discussed in terms of molecular design,detection selectivity and quantification *** work summarized here provides fundamental guidelines for CPE-based label-free visual sensing.

关键词： conjugated polyelectrolyte heparin fluorescence resonance energy transfer sensor

Growth behavior and electronic regulation of Pt on various Mo-based supports for hydrogen evolution electrocatalysis

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Science China Chemistry 2025年

作者： Lulu Chen Yichao Huang Jiajun Wang Meihong Liao Zhiyuan Liu Meiqi Liu Hanqing Cai Lin Wang Lidong Gao Dawei Hu Lianming Zhao Ning pu Zhuangjun Fan State Key Laboratory of Chemical Safety School of Materials Science and Engineering China University of Petroleum (East China) School of Mechanical and Electronic Engineering Qingdao Binhai University Qingdao Chuangqi New Energy Catalysis Technology Co.Ltd. Sinopec Research Institute of Petroleum Processing Co.Ltd. Institute of Energy Hefei Comprehensive National Science Center

Insight into the growth behavior and electronic regulation of platinum （Pt） on various transition metal supports is paramount in developing high-performing electrocatalysts for hydrogen evolution reactions （HER）.Herein,we studied the influence of molybdenum-based supports （MoX,X=C,N,P,and S） on the growth behavior and electronic regulation of *** found that the formation energy variations between Pt single atom and clusters on MoX supports play a pivotal role in the growth behavior of ***,the electronic regulation of Pt induced by metal-support interaction may reflect the valence changes of Pt in Pt-MoX/*** Pt-MoC/C catalyst with a moderate valence state of Pt exhibits the best HER activity with an overpotential of 12.0 mV at 10 mA cm-2and a mass activity of 27.1 A mgPt-1,12.3 times as high as that of commercial 20 wt% Pt/*** work provides constructive guidance for the design of high-performance HER catalysts.

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Polyoxometalate-encapsulated metal-organic frameworks with diverse cages for the C-H bond oxidation of alkylbenzenes

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Chinese Journal of Structural Chemistry 2023年第2期42卷 35-42页

作者： Yue Wang Ze-Xuan Liu Xing-pu Zhao Yuan-Yuan Ma Si-Meng Zhang wen-jing Cui jing Du Zhan-Gang Han Hebei Key Laboratory of Organic Functional Molecules Hebei Key Laboratory of Inorganic NanomaterialsNational Demonstration Center for Experimental Chemistry EducationCollege of Chemistry and Materials ScienceHebei Normal UniversityShijiazhuangHebei050024PR China Testing and Analysis Center Hebei Normal UniversityShijiazhuangHebei050024PR China

Exploring new heterogeneous catalysts to achieve efficient C-H bond oxidation is momentous in industrial chemical ***,three Fe-incorporated polyoxometalate-encapsulated metal-organic frameworks(POM@MOFs),[Fe(H_(2)O)_(3)(dtb)][Fe(dtb)_(2)][HBW_(12)O_(40)]-12H_(2)O(1),[Fe(H_(2)O)2(dtb)]_(2)[Fe(dtb)_(2)(Hdtb)][SiW^(Ⅵ)_(9)W^(Ⅴ)_(3)O_(40)]·16H_(2)O(2),[Fe(H_(2)O)_(2)]_(4)(dtb)_(5)[PMo^(Ⅵ)_(11)Mo^(Ⅴ)O_(40)]_(2)·18H_(2)O(3),(dtb=1,4-di[4H-1,2,4-triazol-4-yl]-benzene),were hydrothermally synthesized for catalytic C-H bond *** exhibited three-dimensional(3-D)POM-based metal-organic host-guest frameworks with diverse *** compound 1,cationic metal-organic frameworks constructed by binuclear[Fe_(2)(dtb)_(6)(H_(2)O)_(2)]^(4+)clusters and dtb ligands present a pcu alpha-Po primitive cubic topological net and abundant twisted quadrangular prism-shaped cages,in which the monoprotonated[HBW_(12)O_(40)]^(4-)polyoxoanions are *** compound 2,two-dimensional(2-D)cationic metal-organic layers constructed by trinuclear[Fe_(3)(dtb)_(6)(H_(2)O)_(4)]^(6+)clusters and dtb ligands display a sql/Shubnikov tetragonal plane net topology,in which the rhomboid-shaped metal-organic windows in adjacent parallel-arranged 2-D layers are enclosed to form an open parallelepiped ***[SiW^(Ⅵ)_(9)W^(Ⅴ)_(3)O_(40)]^(7-)poly-oxoanions locate in the parallelepiped cages between 2-D *** compound 3,the 3-D cationic metal-organic frameworks constructed by binuclear[Fe_(2)(dtb)_(3)(H_(2)O)_(4)]^(4+)clusters and dtb ligands possess a bnn hexagonal BN topology,in which the hexagonal prismatic metal-organic cages accommodate four[PMo^(Ⅵ)_(11)Mo^(Ⅴ)O_(40)]^(4-)*** diphenylmethane(DPM)oxidation as C-H bond oxidation model reaction,compounds 1-3 displayed distinct catalytic activities owing to the synergistic effect of polynuclear Fe nodes and *** them,[PMo^(Ⅵ)_(11)Mo^(Ⅴ)O_(40)]^(4-)-contained compound 3 exhibited higher catalytic

关键词： Polyoxometalate Metal-organic framework Heterogeneous catalysis C-H bond oxidation

Nicotine up-regulates SLC7A5 expression depending on TRIM29 in non-small cell lung cancer

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Genes & Diseases 2024年第2期11卷 582-584页

作者： Dahua Liu Haolin Ren Guimin wen pu Xia Biological Anthropology Institute College of Basic Medical ScienceJinzhou Medical UniversityJinzhouLiaoning 121000China Department of Radiology The First Affiliated Hospital of Dalian Medical UniversityDalianLiaoning 116011China Department of Community Nursing College of NursingJinzhou Medical UniversityJinzhouLiaoning 121000China

Non-small cell lung cancer(NSCLC)is a malignant tumor that poses a serious threat to human ***(LUAD)and squamous cell carcinoma(LUSC)are two common types of *** originate from glandular and squamous cells of the bronchial epithelium,*** gene changes during tumorigenesis is conducive to the selection of suitable treatment methods for ***98 is a heterodimeric transmembrane glycoprotein,which is composed of a heavy chain and a light chain.1 cD98 heavy chain(CD98hc),also known as 4F2hc or SLC3A2,is an 85kDa type ll transmembraneglycoprotein,which consists of a cytoplasmic region(NH2 terminal),a single chain transmembrane region,and a huge extracellular region(COOH terminal).1 CD98hc can combine with the CD98 light chain,LAT1(SLC7A5),to form CD98 protein through disulfide bonds.1 SLC7A5 is a 12 times transmembrane helix bundle protein composed of 501-535 amino acid residues1.

关键词： lung cancer NSCLC

Magnesium ion influx reduces neuroinflammation in Aβ precursor protein/Presenilin 1 transgenic mice by suppressing the expression of interleukin-1β

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Cellular & Molecular Immunology 2017年第5期14卷 451-464页

作者： pu Wang Xin Yu Pei-Pei Guan jing-wen Guo Yue Wang Yan Zhang Hang Zhao Zhan-You Wang College of Life and Health Sciences Northeastern University Shenyang P. R. China

Alzheimer＇s disease （AD） has been associated with magnesium ion （Mg2＋） deficits and interleukin-1β（IL-1β） elevations in the serum or brains of AD patients. However, the mechanisms regulating IL-1β expression during Mg2＋ dyshomeostasis in AD remain unknown. We herein studied the mechanism of IL-1β reduction using a recently developed compound, magnesium-L-threonate （MgT）. Using human glioblastoma A172 and mouse brain DIA glial cells as an in vitro model system, we delineated the signaling pathways by which MgT suppressed the expression of IL-1β in glial cells. In detail, we found that MgT incubation stimulated the activity of extracellular signal-regulated protein kinases I and 2 （ERK1/2） and peroxisome proliferator-activated receptor gamma （PPARγ） signaling pathways by phosphorylation, which resulted in IL-1β suppression. Simultaneous inhibition of the phosphorylation of ERK1/2 and PPARγ, induced IL-1β upregulation in MgT-stimulated glial cells. In accordance with our in vitrodata, the intracerebroventricular （i.c.v） injection of MgT into the ventricles of APP/PS1 transgenic mice and treatment of Aβ precursor protein （APP）/PS1 brain slices suppressed the mRNA and protein expression of IL-1β. These in vivo observations were further supported by the oral administration of MgT for 5 months. Importantly, Mg2＋ influx into the ventricles of the mice blocked the effects of IL-1β or amyloid p-protein oligomers in the cerebrospinal fluid. This reduced the stimulation of IL-1β expression in the cerebral cortex of APP/PS1 transgenic mice, which potentially contributed to the inhibition of neuroinflammation.

关键词： Alzheimer's disease amyloid β-protein magnesium-L-threonate PPARγ,

NUMERICAL SIMULATIONS OF WAVE-INDUCED SHIP MOTIONS IN TIME DOMAIN BY A RANKINE PANEL METHOD

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Journal of Hydrodynamics 2010年第3期22卷 373-380页

作者： CHEN jing-pu ZHU De-xiang China Ship Scientific Research Center Shanghai 214082 China Shanghai Institute of Computing Technology Shanghai 200040 China

A time domain prediction of wave-induced ship motions by a Rankine panel method is investigated. Linear boundary conditions on free surface and mean wetted body surface are adopted, while the numerical damping method is used for the radiation conditions. The motions of two ships in regular head waves are computed by the present method. The related numerical results are compared with the experiment data and those from linear strip theory. The comparison shows satisfactory agreements for pitch and heave transfer functions.

关键词： Rankine source panel method time domain ship motion forward speed