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小麦低磷响应基因的筛选与表达分析

中国农业大学学报 2016年第10期21卷 1-10页

作者：尚文静贾利华史磊林德立刘娜郑文明河南农业大学生命科学学院/小麦玉米作物学国家重点实验室/河南粮食作物协同创新中心郑州450002

利用Affymetrix wheat microarray分析了郑麦9023在低磷和正常磷水培条件下,苗期根部基因在转录水平的差异。结果表明,1)筛选得到1 889个差异表达基因,其中1 298个上调表达,591个下调表达。2)基因功能分析显示,这1 889个基因参与了信号... 详细信息

利用Affymetrix wheat microarray分析了郑麦9023在低磷和正常磷水培条件下,苗期根部基因在转录水平的差异。结果表明,1)筛选得到1 889个差异表达基因,其中1 298个上调表达,591个下调表达。2)基因功能分析显示,这1 889个基因参与了信号转导、蛋白存储、逆境胁迫、能量代谢、病程相关和其他与植物生长发育有关的过程。3)利用qRT-PCR对其中30个基因的表达情况进行了验证,显示与基因芯片结果基本相同的表达趋势。4)在低磷及磷恢复条件下,对其中5个候选基因(TaSPX3,TaIPS1.3,TaGST_like,TaPDF19,TaG6PDH1_like)在不同小麦品种中的表达进行分析,显示这5个基因受到低磷胁迫的强烈诱导,恢复供磷后表达显著回调。但是,这些候选基因在不同品种间横向表达趋势有差别,反应了不同基因型小麦对低磷胁迫的响应机制存在差异。预测这5个候选基因的功能涉及信号转导、氨基酸代谢、糖代谢、抗逆响应等重要的代谢或调控途径,在小麦应对低磷逆境机制中发挥重要作用。

关键词：小麦 Affymetrix wheat microarray 低磷胁迫差异表达基因 qRT-PCR

AZF gene expression analysis in peripheral leukocytes and testicular cells from idiopathic infertility

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AZF gene expression analysis in peripheral leukocytes and te...

Small extracellular vesicles derived from human induced pluripotent stem cell-differentiated neural progenitor cells mitigate retinal ganglion cell degeneration in a mouse model of optic nerve injury

第十五届全国泌尿外科学术会议

作者： SONG Ning-hong, WU Hong-fei , ZHANG Wei, ZHUO Zuo-min, DING Guan-xiong, ZHANG jing, hua li-xin Department of Urology, First Afficiated Hospital, Nanjing Medical University, Nanjing210029,China

The aim of this study was to assess the frequency of microdeletions in Chinese men with idiopathic infertility and to investigate whether there was a difference on AZF gene expression between peripheral leukocytes and testicular cells. In this study, we screened 62 idiopathic infertile patients, in whom karyotype, sperm count and hormonal parameters were evaluated. Genomic DNA was extracted from the peripheral leukocytes. Molecular analysis was performed by two multiplex polymerase chain reactions (PCR) using a set of eight sequence tagged sites (STS) from 3 different regions of the Y chromosome. Total cellular RNA was extracted from the testicular tissue using a Trizol-method. Reverse Transcription (RT) reactions were performed to synthesize cDNA. Amplification of DFFRY, RBM and DAZ genes is performed to analyze their expression in testicular cells. In this cohort, we found 12 submicroscopic deletions (12/62, 19.4%). Nine patients (9/33, 27.2%) were detected in the azoospermic group and three (3/29, 10.3%) in the severe oligozoospermic group. RT-PCR analysis from testicular cells gave normal amplifications for SRY and DFFRY mRNA in 62 idiopathic patients;two patients were negative for RBM expression;No RBM and DAZ were detected for a case;12 patients had no expression in the AZFc region involving the DAZ gene. Of 12 cases, three patients with normal PCR analysis of DAZ gene on genomic DNA showed no RT-PCR amplification for DAZ mRNA. In conclusion, microdeletions of the Y chromosome are an important cause for idiopathic oligo- or azoospermia. Multiplex PCR is a useful technique for detecting the microdeletions. In addition, although subjects presented a normal PCR analysis of Yq on genomic DNA extracted from peripheral leukocytes, by RT- PCR, no AZF expression is possible in testicular material. Therefore, the combination of RT-PCR evaluation of specific spermatids expressed genes with PCR blood evaluation might raise the odds for successful TESE (Testicular sperm a

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Neural Regeneration Research 2025年第2期20卷 587-597页

作者： Tong li Hui-Min Xing Hai-Dong Qian Qiao Gao Sheng-Lan Xu hua Ma Zai-Long Chi State Key Laboratory of Ophthalmology Optometry and Visual ScienceEye HospitalWenzhou Medical UniversityWenzhouZhejiang ProvinceChina National Clinical Research Center for Ocular Diseases Eye HospitalWenzhou Medical UniversityWenzhouZhejiang ProvinceChina

Several studies have found that transplantation of neural progenitor cells(NPCs)promotes the survival of injured ***,a poor integration rate and high risk of tumorigenicity after cell transplantation limits their clinical *** extracellular vesicles(sEVs)contain bioactive molecules for neuronal protection and *** studies have shown that stem/progenitor cell-derived sEVs can promote neuronal survival and recovery of neurological function in neurodegenerative eye diseases and other eye *** this study,we intravitreally transplanted sEVs derived from human induced pluripotent stem cells(hiPSCs)and hiPSCs-differentiated NPCs(hiPSC-NPC)in a mouse model of optic nerve *** results show that these intravitreally injected sEVs were ingested by retinal cells,especially those localized in the ganglion cell *** with hiPSC-NPC-derived sEVs mitigated optic nerve crush-induced retinal ganglion cell degeneration,and regulated the retinal microenvironment by inhibiting excessive activation of *** analysis further revealed that hiPSC-NPC derived sEVs transported neuroprotective and anti-inflammatory miRNA cargos to target cells,which had protective effects on RGCs after optic nerve *** findings suggest that sEVs derived from hiPSC-NPC are a promising cell-free therapeutic strategy for optic neuropathy.

关键词： exosome miRNA neural progenitor cell neurodegeneration neuroinflammation neuroprotection optic nerve crush optic neuropathy retinal ganglion cell small extracellular vesicles

Low‑Temperature Oxidation Induced Phase Evolution with Gradient Magnetic Heterointerfaces for Superior Electromagnetic Wave Absorption

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Nano-Micro Letters 2025年第1期17卷 191-204页

作者： Zizhuang He lingzi Shi Ran Sun lianfei Ding Mukun He Jiaming li hua Guo Tiande Gao Panbo liu School of Chemistry and Chemical Engineering Northwestern Polytechnical UniversityXi’an 710129People’s Republic of China School of Marine Science and Technology Northwestern Polytechnical UniversityXi’an 710072People’s Republic of China

Gradient magnetic heterointerfaces have injected infinite vitality in optimizing impedance matching,adjusting dielectric/magnetic resonance and promoting electromagnetic(EM)wave absorption,but still exist a significant challenging in regulating local phase ***,accordion-shaped Co/Co_(3)O_(4)@N-doped carbon nanosheets(Co/Co_(3)O_(4)@NC)with gradient magnetic heterointerfaces have been fabricated via the cooperative high-temperature carbonization and lowtemperature oxidation *** results indicate that the surface epitaxial growth of crystal Co_(3)O_(4) domains on local Co nanoparticles realizes the adjustment of magnetic-heteroatomic components,which are beneficial for optimizing impedance matching and interfacial ***,gradient magnetic heterointerfaces simultaneously realize magnetic coupling,and long-range magnetic ***,the synthesized Co/Co_(3)O_(4)@NC absorbents display the strong electromagnetic wave attenuation capability of−53.5 dB at a thickness of 3.0 mm with an effective absorption bandwidth of 5.36 GHz,both are superior to those of single magnetic domains embedded in carbon *** design concept provides us an inspiration in optimizing interfacial polarization,regulating magnetic coupling and promoting electromagnetic wave absorption.

关键词： Magnetic heterointerfaces Phase evolution Interfacial polarization Magnetic coupling Electromagnetic wave absorption

Single-cell omics: experimental workflow, data analyses and applications

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Science China life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan li Dongqing Sun Shaliu Fu Lei Gu Xin Shao Qinqin Wang Xin Dong Bin Duan Feiyang Xing Jun Wu Minmin Xiao Fangqing Zhao jing-Dong J.Han Qi liu Xiaohui Fan Chen li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of life Science Chinese Academy of Sciences Peking-Tsinghua Center for life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

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浅析大学生创业能力的培养途径

无锡商业职业技术学院学报 2010年第3期10卷 46-48页

作者：张静敬丽华包忠明常州纺织服装职业技术学院江苏常州213000

21世纪是一个创新的世纪,社会迫切需要具有创新创业能力的人才。高等院校担负着培养高素质人才的重任,必须积极探寻大学生创业能力的培养途径。文章从四个方面提出了大学生创业能力的培养途径:构建创业教育课程体系、充分运用高校各种... 详细信息

21世纪是一个创新的世纪,社会迫切需要具有创新创业能力的人才。高等院校担负着培养高素质人才的重任,必须积极探寻大学生创业能力的培养途径。文章从四个方面提出了大学生创业能力的培养途径:构建创业教育课程体系、充分运用高校各种资源帮助学生形成合理的创业知识结构、积极组织创业实践活动、广泛宣传大学生创业政策。

关键词：大学生创业能力培养途径

Deciphering Water Oxidation Catalysts: The Dominant Role of Surface Chemistry over Reconstruction Degree in Activity Promotion

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NANO-MICRO LETTERS 2025年第1期17卷 1-15页

作者： An, li li, Jianyi Sun, Yuanmiao Zhu, Jiamin Seow, Justin Zhu Yeow Zhang, Hong Zhang, Nan Xi, Pinxian Xu, Zhichuan J. Yan, Chun-hua Lanzhou Univ Frontiers Sci Ctr Rare Isotopes Key Lab Nonferrous Met Chem & Resources Utilizat G State Key Lab Appl Organ ChemColl Chem & Chem Eng Lanzhou 730000 Peoples R China Nanyang Technol Univ Sch Mat Sci & Engn Singapore 639798 Singapore Chinese Acad Sci Fac Mat Sci & Energy Engn Shenzhen Inst Adv Technol Inst Technol Carbon Neutral Shenzhen 518055 Peoples R China Yunnan Univ Natl Ctr Int Res Photoelect & Energy Mat Electron Microscopy Ctr Sch Mat & EnergyKey Lab Electromagnet Mat & Devic Kunming 650091 Peoples R China Baotou Res Inst Rare Earths State Key Lab Baiyunobo Rare Earth Resource Res & Baotou 014030 Peoples R China Peking Univ Beijing Natl Lab Mol Sci State Key Lab Rare Earth Mat Chem & Applicat PKU HKU Joint Lab Rare Earth Mat & Bioinorgan Chem Beijing 100871 Peoples R China

Demonstrate that the key factor to determine the activity of reconstructed surfaces is the surface chemistry, instead of reconstruction degree using the popular perovskite LaNi1-xFexO3 oxides as model *** content can influence both the surface reconstruction degree, the activation degree, and the activity of reconstructed *** oxygen evolution reaction activity of reconstructed catalysts is primarily governed by the chemistry of the reconstructed surface species. Water splitting hinges crucially on the availability of electrocatalysts for the oxygen evolution reaction. The surface reconstruction has been widely observed in perovskite catalysts, and the reconstruction degree has been often correlated with the activity enhancement. Here, a systematic study on the roles of Fe substitution in activation of perovskite LaNiO3 is reported. The substituting Fe content influences both current change tendency and surface reconstruction degree. LaNi0.9Fe0.1O3 is found exhibiting a volcano-peak intrinsic activity in both pristine and reconstructed among all substituted perovskites in the LaNi1-xFexO3 (x = 0.00, 0.10, 0.25, 0.50, 0.75, 1.00) series. The reconstructed LaNi0.9Fe0.1O3 shows a higher intrinsic activity than most reported NiFe-based catalysts. Besides, density functional theory calculations reveal that Fe substitution can lower the O 2p level, which thus stabilize lattice oxygen in LaNi0.9Fe0.1O3 and ensure its long-term stability. Furthermore, it is vital interesting that activity of the reconstructed catalysts relied more on the surface chemistry rather than the reconstruction degree. The effect of Fe on the degree of surface reconstruction of the perovskite is decoupled from that on its activity enhancement after surface reconstruction. This finding showcases the importance to customize the surface chemistry of reconstructed catalysts for water oxidation.

关键词： Oxygen evolution reaction Perovskite oxides Doping Activation and reconstruction

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282种中成药日均价格的分层调查与分析

中国医院用药评价与分析 2009年第11期9卷 878-880页

作者：景丽华赵志刚首都医科大学附属北京天坛医院药剂科北京市100050

目的：对我院282种中成药日均价格进行统计分析，为医院从经济角度管理药品，为临床医师合理、经济地选择药品提供参考依据，使患者的利益最大化。方法：采用医院计算机网络系统的数据信息，计算出282种中成药的日均价格，进行分层比较... 详细信息

目的：对我院282种中成药日均价格进行统计分析，为医院从经济角度管理药品，为临床医师合理、经济地选择药品提供参考依据，使患者的利益最大化。方法：采用医院计算机网络系统的数据信息，计算出282种中成药的日均价格，进行分层比较分析。结果：日均价格在5元以下的品种占44.24％，日均价格在5-10元的品种占37.05％，日均价格在10-20元的品种占12.59％，日均价格在20-30元的品种占3.24％，日均价格在40-50元、50-60元、60-70元范围的品种各1种，日均价格在150元以上的品种有1种。结论：中成药日均价格在10元以下的品种占81.29％，日均价格最高的中成药在150元以上。为肿瘤治疗药物，其次为骨伤科药物；日均价格最低的中成药只有0.43元，为妇科用药。中成药日均价格的高低与所治疗的疾病的种类直接相关，同时药物剂型也是影响日均价格的重要因素之一。

关键词：中成药日均价格价格层次

基于GIS-ESDA的西北地区区域经济空间差异研究

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山西建筑 2014年第13期40卷 251-253页

作者：张旺锋景丽华兰州大学资源环境学院甘肃兰州730000

以西北地区为研究对象,基于探索性空间数据分析方法(ESDA),分析了区域经济发展的空间差异性,并对西北地区经济发展策略做出思考,提出从制定差异化发展对策、培育以省会城市为核心的城市群、引导基础设施优先建设三方面促进西北地区的经... 详细信息

以西北地区为研究对象,基于探索性空间数据分析方法(ESDA),分析了区域经济发展的空间差异性,并对西北地区经济发展策略做出思考,提出从制定差异化发展对策、培育以省会城市为核心的城市群、引导基础设施优先建设三方面促进西北地区的经济发展。

关键词：探索性空间数据分析空间集聚差异性分析