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Hearing impairment-associated KARS mutations lead to defects in aminoacylation of both cytoplasmic and mitochondrial tRNA^(Lys)

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Science China(Life Sciences) 2020年第8期63卷 1227-1239页

作者： Yong Wang jing-bo zhou Qi-Yu Zeng Siqi Wu Mei-Qin Xue Pengfei Fang En-Duo Wang Xiao-Long zhou State Key Laboratory of Molecular Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China State Key Laboratory of Bioorganic and Natural Products Chemistry Center for Excellence in Molecular SynthesisShanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 200032China

Aminoacyl-tRNA synthetases(aaRSs)are ubiquitously expressed,essential enzymes,synthesizing aminoacyl-tRNAs for protein *** defects of aaRSs frequently cause various human *** KARS encodes both cytosolic and mitochondrial lysyl-tRNA synthetases(LysRSs).Previously,two mutations(c.1129 G>A and c.517 T>C)were identified that led to hearing impairment;however,the underlying biochemical mechanism is *** the present study,we found that the two mutations have no impact on the incorporation of LysRS into the multiple-synthetase complex in the cytosol,but affect the cytosolic LysRS level,its tertiary structure,and cytosolic tRNA aminoacylation in *** for mitochondrial translation,the two mutations have little effect on the steady-state level,mitochondrial targeting,and tRNA binding affinity of mitochondrial ***,they exhibit striking differences in charging mitochondrial tRNALys,with the c.517T>C mutant being completely deficient in vitro and in *** constructed two yeast genetic models,which are powerful tools to test the in vivo aminoacylation activity of KARS mutations at both the cytosolic and mitochondrial ***,our data provided biochemical insights into the potentially molecular pathological mechanism of KARS c.1129G>A and c.517T>C mutations and provided yeast genetic bases to investigate other KARS mutations in the future.

关键词： aminoacyl-tRNA synthetase tRNA aminoacylation protein synthesis

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腺苷酸激酶基因在大肠杆菌中的可溶性高表达

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生物化学与生物物理进展 1997年第6期24卷 525-528页

作者：静恩周波罗杰张洪杰中国科学院生物物理研究所北京100101

报道了鸡肌腺苷酸激酶基因的克隆和在温控启动子PRPL 调控下在大肠杆菌中的可溶性高效表达 .SDS PAGE分析表明 ,鸡肌腺苷酸激酶的含量可占大肠杆菌细胞总蛋白含量的 38% .利用John son等的干冰 /乙醇冰水浴反复冻融法 ,可将此重组蛋白... 详细信息

报道了鸡肌腺苷酸激酶基因的克隆和在温控启动子PRPL 调控下在大肠杆菌中的可溶性高效表达 .SDS PAGE分析表明 ,鸡肌腺苷酸激酶的含量可占大肠杆菌细胞总蛋白含量的 38% .利用John son等的干冰 /乙醇冰水浴反复冻融法 ,可将此重组蛋白进行富集 ,纯度可达 85%以上 .

关键词：腺苷酸激酶大肠杆菌可溶性高表达基因表达

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Application of the improved dung beetle optimizer,muti-head attention and hybrid deep learning algorithms to groundwater depth prediction in the Ningxia area,China

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Atmospheric and Oceanic Science Letters 2025年第1期18卷 18-23页

作者： Jiarui Cai bo Sun Huijun Wang Yi Zheng Siyu zhou Huixin Li Yanyan Huang Peishu Zong Collaborative Innovation Center on forecast and Evaluation of Meteorological Disasters/Key Laboratory of Meteorological Disaster Ministry of Education/Joint International Research Laboratory of Climate and Environment ChangeNanjing University of Information Science and TechnologyNanjingChina Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) ZhuhaiChina China Meteorological Administration Key Laboratory of Transportation MeteorologyNanjingChina Jiangsu Meteorological Observatory NanjingChina

Due to the lack of accurate data and complex parameterization,the prediction of groundwater depth is a chal-lenge for numerical *** learning can effectively solve this issue and has been proven useful in the prediction of groundwater depth in many *** this study,two new models are applied to the prediction of groundwater depth in the Ningxia area,*** two models combine the improved dung beetle optimizer(Dbo)algorithm with two deep learning models:The Multi-head Attention-Convolution Neural Network-Long Short Term Memory networks(MH-CNN-LSTM)and the Multi-head Attention-Convolution Neural Network-Gated Recurrent Unit(MH-CNN-GRU).The models with Dbo show better prediction performance,with larger R(correlation coefficient),RPD(residual prediction deviation),and lower RMSE(root-mean-square error).Com-pared with the models with the original Dbo,the R and RPD of models with the improved Dbo increase by over 1.5%,and the RMSE decreases by over 1.8%,indicating better prediction *** addition,compared with the multiple linear regression model,a traditional statistical model,deep learning models have better prediction performance.

关键词： Groundwater depth Multi-head attention Improved dung beetle optimizer CNN-LSTM CNN-GRU Ningxia

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A Novel Self-Supervised Learning Network for Binocular Disparity Estimation

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Computer Modeling in Engineering & Sciences 2025年第1期142卷 209-229页

作者： Jiawei Tian Yu zhou Xiaobing Chen Salman A.AlQahtani Hongrong Chen bo Yang Siyu Lu Wenfeng Zheng Department of Computer Science and Engineering Major in Bio Artificial IntelligenceHanyang UniversityAnsan-si15577Republic of Korea School of Electrical and Computer Engineering Louisiana State UniversityBaton RougeLA 70803USA Department of Computer Engineering College of Computer and Information SciencesKing Saud UniversityRiyadh11574Saudi Arabia School of Automation University of Electronic Science and Technology of ChinaChengdu610054China

Two-dimensional endoscopic images are susceptible to interferences such as specular reflections and monotonous texture illumination,hindering accurate three-dimensional lesion reconstruction by surgical *** study proposes a novel end-to-end disparity estimation model to address these *** approach combines a Pseudo-Siamese neural network architecture with pyramid dilated convolutions,integrating multi-scale image information to enhance robustness against lighting *** study introduces a Pseudo-Siamese structure-based disparity regression model that simplifies left-right image comparison,improving accuracy and *** model was evaluated using a dataset of stereo endoscopic videos captured by the Da Vinci surgical robot,comprising simulated silicone heart sequences and real heart video *** results demonstrate significant improvement in the network’s resistance to lighting interference without substantially increasing ***,the model exhibited faster convergence during training,contributing to overall performance *** study advances endoscopic image processing accuracy and has potential implications for surgical robot applications in complex environments.

关键词： Parallax estimation parallax regression model self-supervised learning Pseudo-Siamese neural network pyramid dilated convolution binocular disparity estimation

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生物质改性酚醛泡沫的研究进展

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化工新型材料 2016年第10期44卷 6-7,10页

作者：梁兵川胡立红周静薄采颖周永红中国林业科学研究院林产化学工业研究所生物质化学利用国家重点实验室国家林业局林产化学工程重点开放性实验室江苏省生物质能源与材料重点实验室南京210042

近年来酚醛泡沫塑料发展迅速,遇到的问题也越来越多。综述了各种生物质材料在酚醛泡沫中的最新研究进展。重点介绍了腰果酚、单宁、木质素、纤维素和生物油等生物质材料在酚醛泡沫塑料中的应用。并对生物质改性酚醛泡沫塑料的应用前景... 详细信息

近年来酚醛泡沫塑料发展迅速,遇到的问题也越来越多。综述了各种生物质材料在酚醛泡沫中的最新研究进展。重点介绍了腰果酚、单宁、木质素、纤维素和生物油等生物质材料在酚醛泡沫塑料中的应用。并对生物质改性酚醛泡沫塑料的应用前景做了展望。

关键词：生物质酚醛泡沫腰果酚单宁木质素纤维素生物油

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小学生德育探究

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林区教学 2017年第9期 79-80页

作者：高靖博邹瑜西藏民族大学

小学生身心发展处于不成熟阶段,尤其是认知上存在一定的局限性,对外界信息缺乏很好的判别能力,加上学校对小学生的德育不够重视,小学生的德育发展受到极大的影响,因此,需要对小学生的德育进行思考与探索。

关键词：小学生德育素质教育

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Effects of aggregate size distribution and carbon nanotubes on the mechanical properties of cemented gangue backfill samples under true triaxial compression

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 311-324页

作者： Qian Yin Fan Wen Zhigang Tao Hai Pu Tianci Deng Yaoyao Meng Qingbin Meng Hongwen jing bo Meng Jiangyu Wu State Key Laboratory of Intelligent Construction and Healthy Operation and Maintenance of Deep Underground Engineering China University of Mining and TechnologyXuzhou 221116China State Key Laboratory of Mining Response and Disaster Prevention and Control in Deep Coal Mines Anhui University of Science and TechnologyHuainan 232001China State Key Laboratory for Geomechanics&Deep Underground Engineering China University of Mining and Technology(Beijing)Beijing 100083China Key Laboratory of Xinjiang Coal Resources Green Mining Ministry of EducationXinjiang Institute of EngineeringUrumqi 830023China

The mechanical behavior of cemented gangue backfill materials(CGBMs)is closely related to particle size distribution(PSD)of aggregates and properties of cementitious ***,the true triaxial compression tests,CT scanning,SEM,and EDS tests were conducted on cemented gangue backfill samples(CGBSs)with various carbon nanotube concentrations(P_(CNT))that satisfied fractal theory for the PSD of *** mechanical properties,energy dissipations,and failure mechanisms of the CGBSs under true triaxial compression were systematically *** results indicate that appropriate carbon nanotubes(CNTs)effectively enhance the mechanical properties and energy dissipations of CGBSs through micropore filling and microcrack bridging,and the optimal effect appears at P_(CNT)of 0.08wt%.Taking PSD fractal dimension(D)of 2.500 as an example,compared to that of CGBS without CNT,the peak strength(σ_(p)),axial peak strain(ε_(1,p)),elastic strain energy(Ue),and dissipated energy(U_(d))increased by 12.76%,29.60%,19.05%,and90.39%,***,excessive CNTs can reduce the mechanical properties of CGBSs due to CNT agglomeration,manifesting a decrease inρ_(p),ε_(1,p),and the volumetric strain increment(Δε_(v))when P_(CNT)increases from 0.08wt%to 0.12wt%.Moreover,the addition of CNTs improved the integrity of CGBS after macroscopic failure,and crack extension in CGBSs appeared in two modes:detour and pass through the ***σ_(p)and U_(d)firstly increase and then decrease with increasing D,and porosity shows the opposite ***ε_(1,p)andΔε_(v)are negatively correlated with D,and CGBS with D=2.150 has the maximum deformation parameters(ε_(1,p)=0.05079,Δε_(v)=0.01990)due to the frictional slip effect caused by coarse *** increasing D,the failure modes of CGBSs are sequentially manifested as oblique shear failure,"Y-shaped"shear failure,and conjugate shear failure.

关键词： cemented gangue backfill materials particle size distribution true triaxial compression test carbon nanotubes mechanical properties failure modes

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永德县古茶树保护和利用研究

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安徽农业科学 2017年第17期45卷 47-49页

作者：常菁施嘉波周玲云南农业大学龙润普洱茶学院云南昆明650201

通过查阅相关文献资料和实地走访调查,对永德县古茶树资源的现状及存在的问题进行了研究。提出应编制永德县古茶树资源保护与合理利用总体规划,成立古茶树资源保护管理专家组,挖掘古茶树民族文化,提升茶叶产品的附价值等措施,以期对永... 详细信息

通过查阅相关文献资料和实地走访调查,对永德县古茶树资源的现状及存在的问题进行了研究。提出应编制永德县古茶树资源保护与合理利用总体规划,成立古茶树资源保护管理专家组,挖掘古茶树民族文化,提升茶叶产品的附价值等措施,以期对永德县古茶树资源进行科学保护管理与合理利用。

关键词：古茶树资源保护合理利用

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Postnatal development of rat retina:a continuous observation and comparison between the organotypic retinal explant model and in vivo development

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Neural Regeneration Research 2025年第3期20卷 900-912页

作者： Baoqi Hu Rui Wang Hanyue Zhang Xiou Wang Sijia zhou bo Ma Yan Luan Xin Wang Xinlin Chen Zhichao Zhang Qianyan Kang Department of Ophthalmology The First Affiliated Hospital of Xi’an Jiaotong UniversityXi’anShaanxi ProvinceChina Department of Ophthalmology The First Affiliated Hospital of Northwest UniversityXi’anShaanxi ProvinceChina Institute of Neurobiology Xi’an Jiaotong University Health Science CenterXi’anShaanxi ProvinceChina

The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell ***,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development ***,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo *** this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in ***,we used SOX2 and PAX6 immunostaining to identify retinal progenitor *** then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,***-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between ***,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,*** timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant *** study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina *** findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.

关键词： bipolar cells differentiation in vivo microglia Müller glia organotypic retinal explant culture postnatal retina development proliferation retinal progenitor cells

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1,1′-二硝氨基-5,5′-偶氮双四唑钾盐的合成、结构及性能

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火炸药学报 2017年第2期40卷 52-56页

作者：李亚南张生勇舒远杰刘亚静王伯周毕福强翟连杰西安近代化学研究所陕西西安710065 氟氮化工资源高效开发与利用国家重点实验室陕西西安710065

以水合肼和叠氮氰为原料,经环化、缩合、氧化偶联、酸解、硝化、中和等反应,合成了1,1′-二硝氨基-5,5′-偶氮双四唑钾盐(K_2DNAABT);利用红外、核磁(~1 H NMR、^(13) C NMR)、元素分析和单晶X射线衍射表征了目标化合物的结构;采用DSC... 详细信息

以水合肼和叠氮氰为原料,经环化、缩合、氧化偶联、酸解、硝化、中和等反应,合成了1,1′-二硝氨基-5,5′-偶氮双四唑钾盐(K_2DNAABT);利用红外、核磁(~1 H NMR、^(13) C NMR)、元素分析和单晶X射线衍射表征了目标化合物的结构;采用DSC和TG方法研究了K_2DNAABT的热性能;基于晶体密度和计算的生成热,采用EXPLO5程序软件预估了K_2DNAABT的爆轰性能。结果表明,K_2DNAABT热分解峰温为194.27°C,晶体密度为2.11g/cm^3,生成热为617.0kJ/mol,爆速为8 367m/s,爆压为31.5GPa,具有较好的热稳定性,优良的爆轰性能,有望作为叠氮化铅的绿色替代物。

关键词：有机化学 1,1'-二硝氨基-5,5'-偶氮双四唑钾盐 K2DNAABT 水合肼叠氮氰

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