检索结果-南通市图书馆

肺痹方干预肺纤维化小鼠肺泡上皮细胞线粒体途径凋亡的机制

在线全文

学校读者我要写书评

暂无评论

中国组织工程研究 2025年第11期29卷 2334-2339页

作者：程雪荆焕熙张运克方泓河南中医药大学河南省郑州市450046 上海中医药大学附属龙华医院上海市200032

背景:研究表明,线粒体介导的肺泡上皮细胞凋亡在肺纤维化发病中起着重要的作用,而肺痹方可以减轻肺纤维化,抑制肺纤维化小鼠细胞外机制转化。目的:探讨肺痹方对博来霉素致肺纤维化小鼠肺泡上皮细胞线粒体途径凋亡的机制。方法:40只C57B... 详细信息

背景:研究表明,线粒体介导的肺泡上皮细胞凋亡在肺纤维化发病中起着重要的作用,而肺痹方可以减轻肺纤维化,抑制肺纤维化小鼠细胞外机制转化。目的:探讨肺痹方对博来霉素致肺纤维化小鼠肺泡上皮细胞线粒体途径凋亡的机制。方法:40只C57BL/6雄性小鼠随机分为空白对照组、模型组、吡非尼酮组、肺痹方组,每组10只。除空白对照组外,其他3组腹腔注射博来霉素[7.5 mg/(kg·d)]建立肺纤维化模型,连续注射10 d。造模后第1天各药物组小鼠灌胃给药[51.43/(kg·d)]吡非尼酮或[12.86 mg/(kg·d)肺痹方],连续给药28 d。用药结束后取材,采用苏木精-伊红染色和Masson染色观察小鼠肺组织的形态学变化,ELISA法检测血清中白细胞介素1、白细胞介素6、白细胞介素17、白细胞介素37水平,Western-blot法检测肺组织中Bax、Bcl-2、Beclin-1和Caspase3表达。结果与结论:①肺组织的形态学观察显示,模型组肺泡间隔及肺泡腔有大量炎症细胞浸润,出现大片融合成团的纤维灶;吡非尼酮组肺泡间隔增厚,炎症细胞少量浸润,出现肺纤维灶;肺痹方组肺泡结构增宽,少量的炎症细胞浸润,肺泡结构几乎无明显受损,少量肺纤维灶。②与空白对照组相比,模型组小鼠血清白细胞介素1、白细胞介素6、白细胞介素17和白细胞介素37质量浓度明显升高(P<0.01),两用药组明显低于模型组(P<0.01),肺痹方组低于非尼酮组。③与空白对照组相比,模型组小鼠肺组织Bax和Caspase3蛋白表达显著升高,两用药组均低于模型组;与空白对照组相比,模型组Bcl-2和Beclin-1蛋白表达显著降低,两用药组均高于模型组。④结论:肺痹方可以减轻肺纤维化,其机制可能与下调白细胞介素1、白细胞介素6、白细胞介素17和白细胞介素37水平,以及调节线粒体凋亡Bax、Bcl-2、Beclin-1和Caspase3相关蛋白从而减少肺泡上皮细胞凋亡有关。

关键词：肺痹方肺纤维化线粒体博来霉素 C57BL/6小鼠

Xanthones from the bark of Garcinia Xanthochymus

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2007年第7期18卷 849-851页

作者： fang fang zhong Yu Chen Zhi Nan Mei Guang zhong Yang Laboratory for Natural Product Chemistry College of Life Sciences South Central University for Nationalities Wuhan 430074 China College of Chemistry and Material Sciences South Central University for Nationalities Wuhan 430074 China

Two novel xanthones, 1,6-dihydroxy-4,5-dimethoxyxanthone （1） and 1,5,6-trihydroxy-7,8-di（3-methyl-2-butenyl）-6＇,6＇-dimethylpyrano（2＇,3＇：3,4）xanthone （2） were isolated from the bark of Garcinia xanthochymus by normal phase and reverse phase silica gel column chromatography. Their structures were elucidated by spectroscopic methods, especially 2D-NMR techniques.

关键词： Garcinia Garcinia xanthochymus Xanthones

在线全文

学校读者我要写书评

暂无评论

植物激素对棉花蕾铃脱落的调控研究进展

作物学报 2025年第1期51卷 1-29页

作者：谢章书谢学方屠小菊刘爱玉董合忠周仲华湖南农业大学农学院/作物生理与分子生物学教育部重点实验室湖南长沙410128 中国热带农业科学院橡胶研究所海南海口571101 山东省农业科学院经济作物研究所山东济南250000

棉花蕾铃脱落是一个普遍存在的现象,有对逆境环境胁迫的主动适应性脱落,也有因品种遗传特性、环境条件、栽培措施以及生物和非生物胁迫被动的受损脱落。蕾铃脱落直接影响着棉花的产量,国内外现有公开报道多集中于20世纪50、60年代关于... 详细信息

棉花蕾铃脱落是一个普遍存在的现象,有对逆境环境胁迫的主动适应性脱落,也有因品种遗传特性、环境条件、栽培措施以及生物和非生物胁迫被动的受损脱落。蕾铃脱落直接影响着棉花的产量,国内外现有公开报道多集中于20世纪50、60年代关于乙烯和脱落酸对棉花蕾铃脱落影响的初步发现。结合其他植物的研究结果来看,蕾铃脱落似乎与生长素、赤霉素和细胞分裂素等促生长类激素的下降以及乙烯和脱落酸等抑制生长类激素的升高密切相关,激素在植物体内不仅介导新陈代谢过程,还协调着与信号通路相关的调控因子,在脱落过程中起着关键作用。本文从植物激素调控入手,综述了近年来包括脱落区形成和作用的分子调控机制以及各激素在棉花蕾铃脱落以及其他植物(生殖)器官脱落过程中的响应和调控机制等。发现目前以棉花蕾铃脱落为对象的研究非常匮乏,且多集中于少数激素对棉花生殖生长影响的有限研究,缺乏对导致蕾铃脱落的深层次机制的探究和解析。因此,未来研究方向应着重于探究棉花蕾铃脱落的遗传基础、发掘新的抗脱落基因资源和培育抗脱落棉花品种,同时加强在以棉花为模型植物下,蕾铃脱落与植物激素调节的关系研究,为提高棉花产量提供理论依据和技术支持。

关键词：棉花植物激素蕾铃脱落研究现状研究方向

Mechanical properties and durability of alkali-activated steel slag-blastfurnace slag cement

同方期刊数据库

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Journal of Iron and Steel Research International 2023年第7期30卷 1342-1355页

作者： jing-xiong zhong Li-ying Cao Mei Li Shu-ping Wang fang Liu Xue-wei Lv Xiao-qin Peng College of Materials Science and Engineering Chongqing UniversityChongqing 400045China Shanghai Baosteel New Building Materials Technology Co. Ltd.Shanghai 200940China Zhoushan Putuo District Housing and Urban Rural Development Bureau Zhoushan 316199ZhejiangChina

Alkali-activated cement(AAC)is either clinker-less or free,and it is also environmentally friendly due to its low carbon footprint and wide range *** wastes,like steel slag and blastfurnace slag,usually have latent hydraulic reactivity,and can be used as precursors of *** clinkerless and clinker-free AAC were prepared from the mixture of steel slag and blastfurnace slag by using water glass as an activator,and four different recipes which satisfied the strength requirement of 42.5R Portland cement were *** recipe of AAC exhibited better resistance to sulfate attack and frost attack than Portland *** showed huge drying shrinkage,but it was equivalent to that of Portland cement as steel slag content increased to 40%.The AAC also had quite low risk of alkali-aggregate *** analysis showed that the major products were calcium silicate hydrate(C–S–H),calcium aluminosilicate hydrate(C–A–S–H)and zeolite-like *** was also detected in the binder when gypsum was contained in the precursors.

关键词： Alkali-activated cement Steel slag Blastfurnace slag Durability Mechanical property

Exact Confidence Limits for the Parameter of an Exponential Distribution in the Accelerated Life Tests under Type-ⅠCensoring

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Mathematicae Applicatae Sinica 2021年第2期37卷 393-408页

作者： De-qiang ZHENG Xiang-zhong fang School of Mathematical Sciences Peking UniversityBejing 100871China

Life data frequently arise in many reliability studies,such as accelerated life tests *** paper considers the part of life data where failure and censoring observations may *** develop statistical methods and theory for the analysis of these data,a new approach was proposed to obtain the exact lower and upper confidence limits for the mean life of the exponential distribution with Type-I censoring *** is assumed that the acceleration factor is a random variable,and that the distribution of the acceleration factor is known from some empirical information or the meta analysis.A method for constructing the lower and upper confidence limits for the parameter based on an ordering relation among the sample space was *** studies and analyses of two examples suggest that the proposed method performed well.

关键词： mean life confidence limit acceleration factor sample space ordering relation

Electroacupuncture Attenuates Ischemic Brain Injury and Cellular Apoptosis via Mitochondrial Translocation of Cofilin

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Integrative Medicine 2021年第9期27卷 705-712页

作者： CHEN Bin LIN Wan-qing LI Zuan-fang zhong Xiao-yong WANG jing YOU Xiao-fang ZHAO Hong-jia QI Da-shi Department of Rehabilitation Medicine and National Clinical Research Base of Traditional Chinese Medicine the Affiliated People's Hospital of Fujian University of Traditional Chinese MedicineFuzhou(350004)China Academy of Integrative Medicine Fujian University of Traditional Chinese MedicineFuzhou(350108)China Shanghai Key Lab of Birth Defect Children's Hospital of Fudan UniversityShanghai(200032)China

Objective:To investigate the potential mechanisms of electroacupuncture(EA)to prevent ischemic ***:The method of middle cerebral artery occlusion(MCAO)was employed to establish a rat model of ischemic ***-eight Sprague-Dawley rats were divided into the sham group,MCAO+EA control(EC)group,and MCAO+EA(EA)group according to a random number table(n=26 per group).EA was applied to the acupoints of Baihui(DU 20)and Shenting(DU 24)5 min and 6 h,respectively after the onset of MCAO for 30 *** in the sham and EC groups received only light isoflurane anesthesia for 30 min after *** neuroprotective effects of EA were evaluated by rota-rod test,neurological deficit scores and infarct ***,Nissl staining and immunostaining were performed to examine brain damage,rod formation,cellular apoptosis,and neuronal loss induced by *** activities of caspase-3,and expression levels of cofilin and p-cofilin in mitochondria and cytoplasm after ischemic injury were determined by Western ***:Compared with the EC group,EA significantly improved neuromotor function and cognitive ability after ischemic stroke(P<0.05 or P<0.01).Therapeutic use of EA also resulted in a significant decrease of cofilin rod formation and microtubule-associated protein-2(MAP2)degradation in the cortical penumbra area compared with the EC rats(P<0.01).Furthermore,Western blot analysis showed that EA stimulation significantly inhibited mitochondrial translocation of cofilin and caspase-3 cleavage(P<0.05 or P<0.01).Additionally,brain damage(infarct volume and neuropathy),cellular apoptosis and neuronal loss induced by ischemia were remarkably suppressed by EA in the cortical penumbra of rats(P<0.05 or P<0.01).Conclusion:EA treatment after ischemic stroke may attenuate ischemic brain injury and cellular apoptosis through the regulation of mitochondrial translocation of cofilin,a novel mechanism of EA therapy.

关键词： electroacupuncture cofilin apoptosis ischemic stroke mitochondrial translocation Chinese medicine

Anti-anaphylactic potential of benzoylpaeoniflorin through inhibiting HDC and MAPKs from Paeonia lactiflora

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2021年第11期19卷 825-835页

作者： zhong Wan-Chao LI En-Can HAO Rui-Rui ZHANG jing-fang JIN Hong-Tao LIN Sheng State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China New Drug Safety Evaluation Center Institute of Materia MedicaChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China Beijing Union-Genius Pharmaceutical Technology Co. Ltd.Beijing 100176China Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing Dongzhimen HospitalBeijing University of Chinese MedicineBeijing 100700China

Guided by cell-based anti-anaphylactic assay,eighteen cage-like monoterpenoid glycosides(1−18)were obtained from the bioactive fraction of *** *** these,compounds 1,5,6,11,12,15,and 17 significantly reduced the release rate ofβ-HEX and HIS without or with less ***,the most potent inhibitor benzoylpaeoniflorin(5)was selected as the prioritized compound for the study of action of mechanism,and its anti-anaphylactic activity was medicated by dual-inhibiting HDC and MAPK signal ***,molecular docking simulation explained that benzoylpaeoniflorin(5)blocked the conversion of L-histidine to HIS by occupying the HDC active ***,in vivo on PCA using BALB/c mice,benzoylpaeoniflorin(5)suppressed the IgE-mediated PCA reaction in antigen-challenged *** findings indicated that cage-like monoterpenoid glycosides,especially benzoylpaeoniflorin(5),mainly contribute to the anti-anaphylactic activity of *** by dual-inhibiting HDC and MAPK signal ***,benzoylpaeoniflorin(5)may be considered as a novel drug candidate for the treatment of anaphylactic diseases.

关键词： Paeonia lactiflora Anti-anaphylactic action RBL-2H3 HDC MAPKs

Microstructure and phase transformation of Ni_(56)Fe_(x)Ga_(44-x)(15≤x≤20)alloys

在线全文

学校读者我要写书评

暂无评论

Rare Metals 2024年第11期43卷 6048-6053页

作者： Cui-fang Dong jing-Min Wang Laboratory of Aerospace Materials and Performance(Ministry of Education) School of Materials Science and EngineeringBeihang UniversityBeijing 100191China

Ni_(56)Fe_(x)Ga_(44-x)(15≤x≤20)alloys were studied with the phase structure and magnetic *** martensitic transformation was observed over the whole composition *** martensitic transformation starting temperature(M_(s))increases from 256 K for x=15 to 350 K for x=18 and then decreases from 350 K for x=18 to333 K for x=20 by increasing substitution of Fe for *** austenitic Curie temperature((?))is observed for15≤x≤16 and 18≤x≤20,*** the martensitic Curie temperature(T^(A)_(X))is observed for x=*** 18

关键词： Ni-Fe-Ga Dual-phase Martensitic transformation Magnetic transition

Theoretical Study on the Catalytic Activity and Sulfur Resistibility of Amorphous Alloy Ni-B-P

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Structural Chemistry 2007年第3期26卷 273-280页

作者： fang Zhi-Gang GUO jing-Xue School of Chemical Engineering University of Science and Technology Liaoning Anshan 114051 China

In the present paper, one hundred cluster models NinBP （n = 1-6） have been designed and studied by density functional theory （DFT） to get an insight into the local structure, catalytic properties and sulfur resistibility of amorphous alloy Ni-B-P. The configurations in triplet state are found more stable than those in the singlet state. It is found： that as the content of Ni in the clusters increases, the value of Fermi level in clusters fluctuated, which shows that the content of Ni can influence the Fermi level to a certain extent. Based on the Fermi level and DOS, we consider the activity of catalyst in hydrogenation reaction is the best in cluster Ni3BP. On the basis of the charge of clusters NinBP （n = 1 -6）, we conclude the amorphous alloy Ni-B-P with high Ni content has better sulfur resistibility and the best hydrogenation activity, strong sulfur resistibility appears in clusters Ni3BP, and the amorphous alloy Ni60B20P20 with similar proportion is expected to prepare in the future.

关键词： amorphous alloy Ni-B-P DFT catalytic activity sulfur resistibility