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Differences of core genes in liver fibrosis and hepatocellular carcinoma:Evidence from integrated bioinformatics and immunohistochemical analysis

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World Journal of Gastrointestinal Oncology 2022年第7期14卷 1265-1280页

作者： Yue Li Shou-Li Yuan jing-ya yin Kun yang Xin-Gang Zhou Wen Xie Qi Wang Department of Pathology Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China Beijing Key Laboratory of Emerging Infectious Diseases Beijing 100015China Key Laboratory of Animal Ecology and Conservation Biology Institute of ZoologyBeijing 100101China Center of Liver Diseases Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China

BACKGROUND Liver fibrosis and hepatocellular carcinoma(HCC)are common adverse consequences of chronic liver *** interaction of various risk factors may cause them to *** of specific biomarkers is of great significance for understanding the occurrence,development mechanisms,and determining the novel tools for diagnosis and treatment of both liver fibrosis and *** To identify liver fibrosis-related core genes,we analyzed the differential expression pattern of core genes in liver fibrosis and *** Gene expression profiles of three datasets,GSE14323,GSE36411,and GSE89377,obtained from the Gene Expression Omnibus(GEO)database,were analyzed,and differentially expressed genes(DEGs)between patients with liver cirrhosis and healthy controls were identified by screening via R software packages and online tool for Venn *** WebGestalt online tool was used to identify DEGs enriched in biological processes,molecular functions,cellular components,and Kyoto Encyclopedia of Genes and Genomes *** protein–protein interactions of DEGs were visualized using Cytoscape with ***,the expression pattern of core genes was analyzed using Western blot and immunohistochemistry in a carbon tetrachloride(CCl_(4))-induced liver cirrhosis mouse model and in patient liver ***,Kaplan-Meier curves were constructed using the Kaplan-Meier plotter online *** Forty-five DEGs(43 upregulated and 2 downregulated genes)associated with liver cirrhosis were identified from three GEO *** hub genes were identified,which were upregulated in liver *** blot and immunohistochemical analyses of the three core genes,decorin(DCN),dermatopontin(DPT),and SRY-box transcription factor 9(SOX9),revealed that they were highly expressed in the CCl4-induced liver cirrhosis mouse *** expression levels of DCN and SOX 9 were positively correlated with the degree of fibrosis,and SOX 9 level in HCC patients was significant

关键词： Liver cirrhosis Hepatocellular carcinoma Bioinformatical analysis Decorin Dermatopontin SRY-box transcription factor 9

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FibroScan-aspartate transaminase:A superior non-invasive model for diagnosing high-risk metabolic dysfunction-associated steatohepatitis

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World Journal of Gastroenterology 2024年第18期30卷 2440-2453页

作者： jing-ya yin Tian-Yuan yang Bing-Qing yang Chen-Xue Hou Jun-Nan Li Yue Li Qi Wang Center of Liver Diseases Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China Department of Pathology Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China Beijing institute of infectious disease Beijing 100015China Beijing Key Laboratory of Emerging Infectious Diseases Institute of Infectious DiseasesBeijing Ditan HospitalCapital Medical UniversityBeijing 100015China

BACKGROUND Non-alcoholic fatty liver disease(NAFLD)with hepatic histological NAFLD activity score≥4 and fibrosis stage F≥2 is regarded as“at risk”non-alcoholic steatohepatitis(NASH).Based on an international consensus,NAFLD and NASH were renamed as metabolic dysfunction-associated steatotic liver disease(MASLD)and metabolic dysfunction-associated steatohepatitis(MASH),respectively;hence,we introduced the term“high-risk MASH”.Diagnostic values of seven non-invasive models,including FibroScan-aspartate transaminase(FAST),fibrosis-4(FIB-4),aspartate transaminase to platelet ratio index(APRI),*** high-risk MASH have rarely been studied and compared in *** To assess the clinical value of seven non-invasive models as alternatives to liver biopsy for diagnosing high-risk *** A retrospective analysis was conducted on 309 patients diagnosed with NAFLD via liver biopsy at Beijing Ditan Hospital,between January 2012 and December *** screening for MASLD and the exclusion criteria,279 patients wereincluded and categorized into high-risk and non-high-risk MASH *** threshold values of each model,sensitivity,specificity,positive predictive value(PPV),and negative predictive values(NPV),were *** operating characteristic curves were constructed to evaluate their diagnostic efficacy based on the area under the curve(AUROC).RESULTS MASLD diagnostic criteria were met by 99.4%patients with *** MASLD population was analyzed in two cohorts:Overall population(279 patients)and the subgroup(117 patients)who underwent liver transient elastography(FibroScan).In the overall population,FIB-4 showed better diagnostic efficacy and higher PPV,with sensitivity,specificity,PPV,NPV,and AUROC of 26.9%,95.2%,73.5%,72.2%,and ***,Forns index,and aspartate transaminase to alanine transaminase ratio(ARR)showed moderate diagnostic efficacy,whereas S index and gamma-glutamyl transpeptidase to platelet ratio(GPR)were relatively *** the sub

关键词： Metabolic dysfunction-associated steatotic liver disease High-risk metabolic dysfunction-associated steatohepatitis Non-invasive models Liver biopsy Diagnostic value

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Single-center retrospective study of the diagnostic value of doubleballoon enteroscopy in Meckel’s diverticulum with bleeding

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World Journal of Gastrointestinal Surgery 2024年第4期16卷 1043-1054页

作者： Tian He Chao yang jing Wang Ji-Sheng Zhong Ai-Hua Li ya-jing yin Li-Ling Luo Chun-Mei Rao Nian-Fen Mao Qiang Guo Zan Zuo Wen Zhang Ping Wan Yunnan Digestive Endoscopy Clinical Medical Center Department of GastroenterologyThe First People’s Hospital of Yunnan ProvinceThe Affiliated Hospital of Kunming University of Science and TechnologyKunming 650032Yunnan ProvinceChina Department of Medicine Kunming University of Science and TechnologyKunming 650093Yunnan ProvinceChina School of Medicine Yunnan University of Traditional Chinese MedicineKunming 650011Yunnan ProvinceChina Department of Gastroenterology Qujing Hospital of Traditional Chinese MedicineQujing 655000Yunnan ProvinceChina Department of Gastroenterology Yunnan University of Traditional Chinese MedicineKunming 650011Yunnan ProvinceChina

BACKGROUND The study aimed to analyze the characteristic clinical manifestations of patients with intestinal disease Meckel’s diverticulum(MD)complicated by digestive tract ***,we aimed to evaluate the value of double-balloon enteroscopy(DBE)in MD diagnosis and the prognosis after laparoscopic diverticula *** To evaluate the value of DBE in the diagnosis and the prognosis after laparoscopic diverticula resection for MD with *** The study retrospectively analyzed relevant data from 84 MD patients treated between January 2015 and March 2022 and recorded their clinical manifestations,auxiliary examination,and follow-up after laparoscopic resection of ***(1)Among 84 MD patients complicated with hemorrhage,77 were male,and 7 were female with an average age of 31.31±10.75 *** incidence was higher in men than in women of different ages;(2)Among the 84 MD patients,65(78.40%)had defecated dark red stools,and 50(58.80%)had no accompanying symptoms during bleeding,indicating that most MD bleeding appeared a dark red stool without accompanying symptoms;(3)The shock index of 71 patients(85.20%)was30%;(4)The DBE-positive rate was 100%(54/54),99mTcpertechnetate-positive scanning rate was 78%(35/45)compared with capsule endoscopy(36%)and small intestine computed tomography(19%).These results suggest that DBE and 99mTc-pertechnetate scans had significant advantages in diagnosing MD and bleeding,especially DBE was a highly precise examination method in MD diagnosis;(5)A total of 54 MD patients with hemorrhage underwent DBE examination before *** endoscopy revealed many mucosal manifestations including normal appearance,inflammatory changes,ulcerative changes,diverticulum inversion,and nodular hyperplasia,with ulcerative changes being the most common(53.70%).This suggests that diverticular mucosal u

关键词： Meckel’s diverticulum with bleeding Double-balloon colonoscopy 99mTc-pertechnetate scanning Capsule endoscopy Ectopic gastric mucosa

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Dynamic Regulation of Hydrogen Bonding Networks and Solvation Structures for Synergistic Solar‑Thermal Desalination of Seawater and Catalytic Degradation of Organic Pollutants

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Nano-Micro Letters 2025年第2期17卷 548-565页

作者： Ming‑Yuan Yu jing Wu Guang yin Fan‑Zhen Jiao Zhong‑Zhen Yu Jin Qu Beijing Key Laboratory of Advanced Functional Polymer Composites Beijing University of Chemical TechnologyBeijing 100029People’s Republic of China State Key Laboratory of Organic‑Inorganic Composites College of Materials Science and EngineeringBeijing University of Chemical TechnologyBeijing 100029People’s Republic of China

Although solar steam generation strategy is efficient in desalinating seawater,it is still challenging to achieve continuous solar-thermal desalination of seawater and catalytic degradation of organic ***,dynamic regulations of hydrogen bonding networks and solvation structures are realized by designing an asymmetric bilayer membrane consisting of a bacterial cellulose/carbon nanotube/Co_(2)(OH)_(2)CO_(3)nanorod top layer and a bacterial cellulose/Co_(2)(OH)_(2)CO_(3)nanorod(BCH)bottom ***,the hydrogen bonding networks inside the membrane can be tuned by the rich surface–OH groups of the bacterial cellulose and Co_(2)(OH)_(2)CO_(3)as well as the ions and radicals in situ generated during the catalysis ***,both SO_(4)^(2−)and HSO_(5)−can regulate the solvation structure of Na^(+)and be adsorbed more preferentially on the evaporation surface than Cl^(−),thus hindering the de-solvation of the solvated Na^(+)and subsequent nucleation/growth of ***,the heat generated by the solar-thermal energy conversion can accelerate the reaction kinetics and enhance the catalytic degradation *** work provides a flow-bed water purification system with an asymmetric solar-thermal and catalytic membrane for synergistic solar thermal desalination of seawater/brine and catalytic degradation of organic pollutants.

关键词： Solar steam generation Seawater desalination Catalytic degradation Bacterial cellulose Cobalt hydroxycarbonate nanorods

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CNKSR2 interactome analysis indicates its association with the centrosome/microtubule system

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Neural Regeneration Research 2025年第8期20卷 2420-2432页

作者： Lin yin yalan Xu Jie Mu Yu Leng Lei Ma Yu Zheng Ruizhi Li yin Wang Peifeng Li Hai Zhu Dong Wang jing Li Institute for Translational Medicine The Affiliated Hospital of Qingdao UniversityMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina School of Basic Medicine Qingdao UniversityQingdaoShandong ProvinceChina College of Life Sciences and School of PharmacyMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina Department of Urology Qingdao Municipal Hospital Affiliated to Qingdao UniversityQingdaoShandong ProvinceChina

The protein connector enhancer of kinase suppressor of Ras 2(CNKSR2),present in both the postsynaptic density and cytoplasm of neurons,is a scaffolding protein with several protein-binding *** of the CNKSR2 gene have been implicated in neurodevelopmental disorders,particularly intellectual disability,although the precise mechanism involved has not yet been fully *** has demonstrated that CNKSR2 plays a role in facilitating the localization of postsynaptic density protein complexes to the membrane,thereby influencing synaptic signaling and the morphogenesis of dendritic ***,the function of CNKSR2 in the cytoplasm remains to be *** this study,we used immunoprecipitation and high-resolution liquid chromatography-mass spectrometry to identify the interactors of *** a combination of bioinformatic analysis and cytological experiments,we found that the CNKSR2 interactors were significantly enriched in the proteome of the *** also showed that CNKSR2 interacted with the microtubule protein DYNC1H1 and with the centrosome marker *** colocalization analysis confirmed the centrosomal localization of *** we downregulated CNKSR2 expression in mouse neuroblastoma cells(Neuro 2A),we observed significant changes in the expression of numerous centrosomal *** manipulation also affected centrosome-related functions,including cell size and shape,cell proliferation,and ***,we found that CNKSR2 interactors were highly enriched in de novo variants associated with intellectual disability and autism spectrum *** findings establish a connection between CNKSR2 and the centrosome,and offer new insights into the underlying mechanisms of neurodevelopmental disorders.

关键词： autism spectrum disorder centrosome CNKSR2 intellectual disability interactome mass spectrometry microtubule neurodevelopmental disease protein complexes protein-protein interactions

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含噪动态光散射测量数据反演中正则化算法与Chahine算法的比较

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光子学报 2016年第11期45卷 153-159页

作者：修文正申晋肖莹莹徐敏王雅静尹丽菊山东理工大学电气与电子工程学院山东淄博255049 山东理工大学光电技术研究所山东淄博255049

采用两种常用的粒度反演方法——正则化和Chahine算法,对90nm与250nm单峰分布、50nm与200nm双峰分布、100nm与300nm双峰分布的模拟动态光散射数据,以及105nm、300nm标准颗粒的实测动态光散射数据进行了反演分析.结果表明:噪声水平的高... 详细信息

采用两种常用的粒度反演方法——正则化和Chahine算法,对90nm与250nm单峰分布、50nm与200nm双峰分布、100nm与300nm双峰分布的模拟动态光散射数据,以及105nm、300nm标准颗粒的实测动态光散射数据进行了反演分析.结果表明:噪声水平的高低是影响粒度分布反演准确性的关键因素之一,反演结果的准确性随噪声水平的增加而降低,噪声水平超过某一阈值后,将无法得到有意义的反演结果;不同反演方法具有不同的抗噪能力,在低噪声水平下反演结果无显著差别,随着噪声水平的增加,反演结果表现出很大差异;正则化方法通过正则参数的选择可以有效抑制噪声影响,表现出强于Chahine算法的抗噪能力;与Chahine算法相比,正则化方法不需要假定初始分布,因此,在噪声较大的实验或生产过程中进行颗粒分布测量时,宜采用正则化方法.

关键词：散射测量粒度分布颗粒测量反问题正则化算法 Chahine算法

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Effects of aggregate size distribution and carbon nanotubes on the mechanical properties of cemented gangue backfill samples under true triaxial compression

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 311-324页

作者： Qian yin Fan Wen Zhigang Tao Hai Pu Tianci Deng yaoyao Meng Qingbin Meng Hongwen jing Bo Meng Jiangyu Wu State Key Laboratory of Intelligent Construction and Healthy Operation and Maintenance of Deep Underground Engineering China University of Mining and TechnologyXuzhou 221116China State Key Laboratory of Mining Response and Disaster Prevention and Control in Deep Coal Mines Anhui University of Science and TechnologyHuainan 232001China State Key Laboratory for Geomechanics&Deep Underground Engineering China University of Mining and Technology(Beijing)Beijing 100083China Key Laboratory of Xinjiang Coal Resources Green Mining Ministry of EducationXinjiang Institute of EngineeringUrumqi 830023China

The mechanical behavior of cemented gangue backfill materials(CGBMs)is closely related to particle size distribution(PSD)of aggregates and properties of cementitious ***,the true triaxial compression tests,CT scanning,SEM,and EDS tests were conducted on cemented gangue backfill samples(CGBSs)with various carbon nanotube concentrations(P_(CNT))that satisfied fractal theory for the PSD of *** mechanical properties,energy dissipations,and failure mechanisms of the CGBSs under true triaxial compression were systematically *** results indicate that appropriate carbon nanotubes(CNTs)effectively enhance the mechanical properties and energy dissipations of CGBSs through micropore filling and microcrack bridging,and the optimal effect appears at P_(CNT)of 0.08wt%.Taking PSD fractal dimension(D)of 2.500 as an example,compared to that of CGBS without CNT,the peak strength(σ_(p)),axial peak strain(ε_(1,p)),elastic strain energy(Ue),and dissipated energy(U_(d))increased by 12.76%,29.60%,19.05%,and90.39%,***,excessive CNTs can reduce the mechanical properties of CGBSs due to CNT agglomeration,manifesting a decrease inρ_(p),ε_(1,p),and the volumetric strain increment(Δε_(v))when P_(CNT)increases from 0.08wt%to 0.12wt%.Moreover,the addition of CNTs improved the integrity of CGBS after macroscopic failure,and crack extension in CGBSs appeared in two modes:detour and pass through the ***σ_(p)and U_(d)firstly increase and then decrease with increasing D,and porosity shows the opposite ***ε_(1,p)andΔε_(v)are negatively correlated with D,and CGBS with D=2.150 has the maximum deformation parameters(ε_(1,p)=0.05079,Δε_(v)=0.01990)due to the frictional slip effect caused by coarse *** increasing D,the failure modes of CGBSs are sequentially manifested as oblique shear failure,"Y-shaped"shear failure,and conjugate shear failure.

关键词： cemented gangue backfill materials particle size distribution true triaxial compression test carbon nanotubes mechanical properties failure modes

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[Recent progress in mass spectrometry imaging using nanospray desorption electrospray ionization]

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Se pu = Chinese journal of chromatography 2025年第1期43卷 43-49页

作者： jing-Bo Wang Xiao-Lan Li Rui-Xia Fan Ping Lü Rui-Chuan yin West China School of Pharmacy Sichuan University Chengdu 610041 China.

Ambient mass spectrometry imaging (MSI) enables hundreds of analytes in tissue sections to be directly mapped at atmospheric pressure with minimal sample preparation. This field is currently experiencing rapid growth, with numerous reported ambient ionization techniques resulting in a "hundred flowers bloom" situation. Nanospray desorption electrospray ionization (nano-DESI), developed by the Laskin group in 2010, is a widely used liquid-extraction-based ambient ionization technique that was first used for mass spectrometry imaging of tissue in 2012. The nano-DESI probe comprises a primary capillary and a nanospray capillary, with the latter efficiently transferring analyte-containing droplets via a tiny liquid bridge formed between the probe and sample surface, thereby enabling nanoelectrospray ionization (nano-ESI) in front of the inlet of a mass spectrometer. The advantages of nano-DESI MSI include minimal sample preparation, high spatial resolution, and high sensitivity. These features are well-suited for imaging various sample types, including frozen tissue sections, microbial communities, and environmental samples. A PubMed-database search using the "nano-DESI" keyword revealed 72 related articles in the 2010-2024 period, with 34 of them published between 2021 and 2024, which indicates that nano-DESI has rapidly developed as an ambient ionization technique over recent years. Herein, we briefly introduce key nano-DESI-MSI research progress reported in the past three years with the aim of better understanding and facilitating the use of this technology. We first discuss advances in ion-source development. Since no commercial nano-DESI source exists, designing and constructing ion sources remain technical challenges that limit its development. Nano-DESI has been successfully coupled with various types of mass spectrometer, including LTQ Orbitrap, quadrupole-Orbitrap (Q Exactive), 6560 IM QTOF, timsTOF Pro2, triple quadrupole, and FTICR. These couplings have signi

关键词： ambient mass spectrometry imaging nanospray desorption electrospray ionization spatial proteomics structural characterization of lipid

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老年局限期食管小细胞癌临床病理特征及预后分析

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中华肿瘤防治杂志 2016年第18期23卷 1254-1259页

作者：王玉栋焦文鹏王军王祎曹峰武亚晶郭银刘青程云杰河北医科大学第四医院肿瘤内科河北石家庄050011 河北医科大学第四医院放疗科河北石家庄050011

目的老年食管癌发病率近年来呈上升的趋势,但关于其临床病理特征及预后因素的研究,尤其是老年食管小细胞癌(primary esophageal small cell of carcinomas,PESC)的研究极为少见。本研究通过探讨老年原发性局限期PESC的临床病理特征及预... 详细信息

目的老年食管癌发病率近年来呈上升的趋势,但关于其临床病理特征及预后因素的研究,尤其是老年食管小细胞癌(primary esophageal small cell of carcinomas,PESC)的研究极为少见。本研究通过探讨老年原发性局限期PESC的临床病理特征及预后影响因素,以期为临床提供参考。方法回顾性分析河北医科大学第四医院2004-01-01-2012-12-31收治的34例经手术切除的原发性老年局限期PESC患者的临床资料,其中单纯手术组19例,术后辅助治疗组15例(手术+化疗9例,手术+放化疗6例)。分析其临床病理特征和淋巴结转移情况,Kaplan-Meier法统计不同治疗方式的局控、远转和生存情况,Cox回归进行多因素分析。结果 17例患者发生淋巴结转移,淋巴结转移率为50.0%(17/34)。41枚发生淋巴结转移,淋巴结转移度为13.1%(41/314)。胸上段PESC上纵隔、中纵隔、下纵隔和腹腔淋巴结转移度分别为28.6%、0、0和0;胸中段PESC分别为16.7%、10.9%、9.4%和16.4%;胸下段PESC分别为0、0、17.4%和24.1%。T3-4期患者淋巴结转移度显著高于T1-2期(20.6%vs 4.5%,χ2=8.050,P=0.005);病变长度≥5cm组淋巴结转移度显著高于<5cm组(17.0%vs 8.0%,χ2=5.611,P=0.018);多因素分析未发现影响淋巴结转移的独立危险因素。单纯手术组患者1、3、5年局控率与生存率分别为82.1%、54.7%、54.7%和42.1%、15.8%、5.3%,术后辅助治疗组分别为86.2%、65.6%、65.6%和93.3%,46.7%,31.1%,两组比较局控率差异无统计学意义(χ2=0.001,P=0.972),术后辅助治疗组生存率优于单纯手术组,χ2=4.105,P=0.043。单纯手术组患者1、3、5年远转率分别为54.3%、71.4%和85.7%,显著高于术后辅助治疗组的13.3%、30.0%和30.0%,χ2=6.632,P=0.010。多因素分析显示,化疗与否是老年局限期PESC生存的独立影响因素,P=0.017。结论老年PESC淋巴结转移程度与转移模式并未随年龄的增长而发生明显变化,淋巴结转移模式与食管鳞癌较为相似,但远处转移率显著高于食管鳞癌。单纯手术治疗预后极差,术后辅助放化疗可延长其生存。系统性化疗在老年患者体质能耐受的情况下仍是治疗基石之选择。

关键词：食管肿瘤小细胞癌肿瘤转移淋巴结治疗模式预后

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Multiple Tin Compounds Modified Carbon Fibers to Construct Heterogeneous Interfaces for Corrosion Prevention and Electromagnetic Wave Absorption

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Nano-Micro Letters 2025年第1期17卷 507-527页

作者： Zhiqiang Guo Di Lan Zirui Jia Zhenguo Gao Xuetao Shi Mukun He Hua Guo Guanglei Wu Pengfei yin College of Science Sichuan Agricultural UniversityYa’an 625014People’s Republic of China School of Materials Science and Engineering Hubei University of Automotive TechnologyShiyan 442002People’s Republic of China Institute of Materials for Energy and Environment State Key Laboratory of Bio‑Fibers and Eco‑TextilesCollege of Materials Science and EngineeringQingdao UniversityQingdao 266071People’s Republic of China Shaanxi Key Laboratory of Macromolecular Science and Technology School of Chemistry and Chemical EngineeringNorthwestern Polytechnical UniversityXi’anShaanxi 710072People’s Republic of China

Currently,the demand for electromagnetic wave(EMW)absorbing materials with specific functions and capable of withstanding harsh environments is becoming increasingly ***-component interface engineering is considered an effective means to achieve high-efficiency EMW ***,interface modulation engineering has not been fully discussed and has great potential in the field of EMW *** this study,multi-component tin compound fiber composites based on carbon fiber(CF)substrate were prepared by electrospinning,hydrothermal synthesis,and high-temperature thermal *** utilizing the different properties of different substances,rich heterogeneous interfaces are *** effectively promotes charge transfer and enhances interfacial polarization and conduction *** prepared SnS/SnS_(2)/SnO_(2)/CF composites with abundant heterogeneous interfaces have and exhibit excellent EMW absorption properties at a loading of 50 wt%in epoxy *** minimum reflection loss(RL)is−46.74 dB and the maximum effective absorption bandwidth is 5.28 ***,SnS/SnS_(2)/SnO_(2)/CF epoxy composite coatings exhibited long-term corrosion resistance on Q235 steel ***,this study provides an effective strategy for the design of high-efficiency EMW absorbing materials in complex and harsh environments.

关键词： Electrostatic spinning Component regulation Heterogeneous interfaces Electromagnetic wave absorption Corrosion protection

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