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The protective role of tacrine and donepezil in the retina of acetylcholinesterase knockout mice

International Journal of Ophthalmology(English edition) 2015年第5期8卷 884-890页

作者： Yun-Min Yi li Cai Yi Shao Man Xu jing-lin Yi Affiliated Eye Hospital of Nanchang University Jiangxi Research Institute of Ophthalmology and Visual Sciences Department of Ophthalmology the First Affiliated Hospital of Nanchang University

AIM: To determine the effect of different concentrations of the acetylcholinesterase (AChE) inhibitors tacrine and donepezil on retinal protection in AChE(+/-) mice (AChE knockout mice) of various ages. METHODS: Cultured ARPE -19 cells were treated with hydrogen peroxide (H2O2) at concentrations of 0, 250, 500, 1000 and 2000 mu mol/L and protein levels were measured using Western blot. Intraperitoneal injections of tacrine and donepezil (0.1 mg/mL, 0.2 mg/mL and 0.4 mg/mL) were respectively given to AChE4- mice aged 2mo and 4mo and wild-type S129 mice for 7d; phosphate buffered saline (PBS) was administered to the control group. The mice were sacrificed after 30d by cardiac perfusion and retinal samples were taken. AChE(+/-) deficient mice were identified by polymerase chain reaction (PCR) analysis using specific genotyping protocols obtained from the Jackson Laboratory website. H&E staining, immunofluorescence and Western blot were performed to observe AChE protein expression changes in the retinal pigment epithelial (RPE) cell layer. RESULTS: Different concentrations of H2O2 induced AChE expression during RPE cell apoptosis. AChE(+/-) mice retina were thinner than those in wild -type mice (Pructure was still intact at 2mo but became thinner with increasing age(P 0.05). CONCLUSION: in viva, tacrine and donepezil can inhibit the expression of AChE; the decrease of AChE expression in the retina is beneficial for the development of the retina.

关键词： retinal pigment epithelium acetylcholinesterase apoptosis AChE(+/-) animal models acetylcholinesterase inhibitors

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Study on temperature characteristics of organic light-emitting diodes based on tris-(8-hydroxylquinoline)-aluminum

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Chinese Science Bulletin 2008年第4期53卷 624-631页

作者： SUO Fan YU JunSheng li WeiZhi LOU Shuangling DENG jing JIANG YaDong State Key Laboratory of Electronic Thin Films and Integrated Devices School of Optoelectronic Information University of ElectronicScience and Technology of China Chengdu 610054 China

Both single-layer and double-layer organic light-emitting devices based on tris-(8-hydroxylquinoline)-aluminum(Alq3) as emitter are fabricated by thermal vacuum *** electroluminescent characteristics of these devices at various temperatures are measured,and the temperature characteristics of device performance are *** effect of temperature on device current conduction regime is analyzed in *** results show that the current-voltage(I-V) characteristics of devices are in good agreement with the theoretical prediction of trapped charge limited current(TCLC).In addition,both the charge carrier mobility and charge carrier concentration in the organic layer increase with the rise of temperature,which results in the monotonous increase of Alq3 device *** current conduction mechanisms of two devices at different temperatures are identical,but the exponent m in current-voltage equation changes randomly with *** device luminance increases slightly and the efficiency decreases monotonously due to the aging of Alq3 luminescent properties caused by high temperature.A tiny blue shift can be observed in the electroluminescent(EL) spectra as the temperature increases,and the reduction of device monochromaticity is caused by the intrinsic characteristics of organic semiconductor energy levels.

关键词：有机发光二极管温度载流子活性三-(8-羟基喹啉)铝

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Impaired autophagy in amyloid-beta pathology:A traditional review of recent Alzheimer's research

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The Journal of Biomedical Research 2023年第1期37卷 30-46页

作者： Minghao Yuan Yangyang Wang Zhenting Huang Feng jing Peifeng Qiao Qian Zou jing li Zhiyou Cai Chongqing Medical University Chongqing 400042China Department of Neurology Chongqing General HospitalUniversity of Chinese Academy of SciencesChongqing 400013China Department of Neurology Chongqing SchoolUniversity of Chinese Academy of SciencesChongqing 400013China Chongqing Key Laboratory of Neurodegenerative Diseases Chongqing 400013China

Alzheimer’s disease(AD) is the most prevalent neurodegenerative disorder. The major pathological changes in AD progression are the generation and accumulation of amyloid-beta(Aβ) peptides as well as the presence of abnormally hyperphosphorylated tau proteins in the brain. Autophagy is a conserved degradation pathway that eliminates abnormal protein aggregates and damaged organelles. Previous studies have suggested that autophagy plays a key role in the production and clearance of Aβ peptides to maintain a steady-state of Aβ peptides ***, a growing body of evidence suggests that autophagy is significantly impaired in the pathogenesis of AD, especially in Aβ metabolism. Therefore, this article reviews the latest studies concerning the mechanisms of autophagy, the metabolism of Aβ peptides, and the defective autophagy in the production and clearance of Aβpeptides. Here, we also summarize the established and new strategies for targeting autophagy in vivo and through clinical AD trials to identify gaps in our knowledge and to generate further questions.

关键词： Alzheimer’s disease autophagy amyloid-beta amyloid precursor protein secretases metabolism

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Radio Channel Measurements and Analysis at 2.4/5GHz in Subway Tunnels

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China Communications 2015年第1期12卷 36-45页

作者： li Jinxing ZHAO Youping ZHANG jing JIANG rui TAO Cheng TAN Zhenhui Institute of Broadband Wireless Mobile Communications School of Electronics and Information EngineeringBeijing Jiaotong University

There is an increasing demand on wireless communications in subway tunnels to provide video surveillance and sensory data for security,maintenance and train control,and to offer various communication or entertainment services(e.g.,Internet,etc.) to passengers as *** wireless channel in tunnels is quite unique due to the confined space and the waveguide ***,modeling the radio channel characteristics in tunnels is critically important for communication systems design or *** paper investigates the key radio channel characteristics of a subway tunnel at 2.4 GHz and 5 GHz,such as the path loss,root mean square(RMS) delay spread,channel stationarity,Doppler shift,and channel *** field measurements show that channel characteristics in tunnels are highly location-dependent and there exist abundant components in Doppler shift *** the straight section of the subway tunnel,the measured path loss exponents are close to1.6,lower than that in free space.

关键词： broadband mobile communication channel measurement channel characteriza-tion subway tunnel

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Apoptosis of human pancreatic carcinoma cell-1 cells induced by Yin Chen Hao Decoction

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World Journal of Gastroenterology 2015年第27期21卷 8352-8357页

作者： Hai-Bo Zhou jing-Ming Chen li-Ming Shao Zhi-Gang Chen Department of Gastroenterology the Second Affiliated Hospital of Zhejiang University School of Medicine

AIM: To evaluate human pancreatic carcinoma cell line(PANC-1) cells apoptosis and Bcl-2 and Bax expression induced by Yin Chen Hao Decoction(YCHD).METHODS: The cell growth inhibitory rate was determined by MTT assay. Apoptosis of PANC-1 cells before and after treatment with YCHD was determined by TUNEL staining. Expression of the apoptosisassociated genes, Bcl-2 and Bax, was detected by immunohistochemical staining and reverse ***: YCHD inhibited the growth of PANC-1 cells. Following treatment with YCHD for 24-96 h, the apoptotic rate of PANC-1 cells increased with time. In addition, the positive rate of Bcl-2 protein expression decreased in a time-dependent manner, whereas the positive rate of Bax protein expression increased in a time-dependent manner. Following treatment of with YCHD for 24-96 h, expression of BAX m RNA increased gradually and BCL-2 m RNA reduced gradually with ***: YCHD induces apoptosis of PANC-1 cells mediated in part via up-regulation of BAX and down-regulation of BCL-2.

关键词： Apoptosis Pancreatic carcinoma Yin ChenHao Decoction

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Determination of gouty arthritis' biomarkers in human urine using reversed-phase high-performance liquid chromatography

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Journal of Pharmaceutical Analysis 2014年第2期4卷 153-158页

作者： Lei-Wen Xiang jing li Jin-Ming lin Hai-Fang li Beijing Key Laboratory of Microanalysis Methods and Instrumentation Department of ChemistryTsinghua University Institute of Food and Fermentation Industries Fuqing Branch of Fujian Normal University

Creatinine, uric acid, hypoxanthine and xanthine are important diagnostic biomarkers in human urine for gouty arthritis or renal disease diacrisis. A simple method for simultaneous determination of these biomarkers in urine based on reversed-phase high-performance liquid chromatography （RP-HPLC） with ultraviolet （UV） detector was proposed. After pretreatment by dilution, centrifugation and filtration, the biomarkers in urine samples were separated by ODS-BP column by elution with methanol/50 mM NaH2PO4 buffer solution at pH 5.26 （5：95）. Good linearity between peak areas and concentrations of standards was obtained for the biomarkers with correlation coefficients in the range of 0.9957-0.9993. The proposed analytical method has satisfactory repeatability （the recovery of data in a range of creatinine, uric acid, hypoxanthine and xanthine was 93.49-97.90%, 95.38-96.45%, 112.46-115.78%and 90.82-97.13%with standard deviation of o5%, respectively） and the limits of detection （LODs, S/N Z 3） for creatinine, uric acid, hypoxanthine, and xanthine were 0.010, 0.025, 0.050 and 0.025 mg/L, respectively. The established method was proved to be simple, accurate, sensitive and reliable for the quantitation of gouty arthritis＇ biomarkers in human urine samples. The ratio of creatinine to uric acid was found to be a possible factor for assessment of gouty arthritis.

关键词： Gouty arthritis Creatinine Uric acid Hypoxanthine Xanthine High-performance liquid chromatography

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Photocatalytic Characterization of TiO_2 Supported on Active Carbon

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Chinese Chemical Letters 2001年第6期12卷 549-550页

作者： Xue Ping li Feng YIN Yuan liN jing Bo ZHANG Xu rui XIAO Laboratory of Photochemistry Center for Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing 100080

The Photocatalytic characterization of TiO2 supported on active carbon was investigated for photocatalytic decomposition of dichloroacetic acid. It was found that TiO2 / AC exhibited a higher photocatalytic activity than pure TiO2. The reason is that active carbon acting as powerful adsorbent supports makes high concentration environments of organic pollutant molecules around TiO2 particles.

关键词： Photocatalysis TiO2 active carbon

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Affective Preferences Mining Approach with Applications in Process Control

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Journal of Shanghai Jiaotong university(Science) 2022年第5期27卷 737-746页

作者： SU Chong LÜjing ZHANG Danyang li Hongguang College of Information Science and Technology Beijing University of Chemical TechnologyBeijing100029China

Traditional industrial process control activities relevant to multi-objective optimization problems,such as proportional integral derivative(PID)parameter tuning and operational optimizations,always demand for process knowledge and human operators’experiences during human-computer ***,the impact of human operators’preferences on human-computer interactions has been rarely highlighted ever *** response to this problem,a novel multilayer cognitive affective computing model based on human personalities and pleasure-arousal-dominance(PAD)emotional space states is established in this ***,affective preferences are employed to update the affective computing model during human-machine ***,we propose affective parameters mining strategies based on genetic algorithms(GAs),which are responsible for gradually grasping human operators’operational preferences in the process control *** routine process control tasks,including PID controller tuning for coupling loops and operational optimization for batch beer fermenter processes,are carried out to illustrate the effectiveness of the contributions,leading to the satisfactory results.

关键词： affective computing preferences mining process control

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PMSM speed control using adaptive sliding mode control based on an extended state observer

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High Technology Letters 2018年第4期24卷 422-433页

作者： liu jing Xia Peipei Deng Yongting li Hongwen Wang Zhiqian Changchun Institute of Optics Fine Mechanics and PhysicsChinese Academy of SciencesChangchun 130033P.R.China University of Chinese Academy of Sciences Beijing 100049P.R.China

In this study,a composite strategy based on sliding-mode control( SMC) is employed in a permanent-magnet synchronous motor vector control system to improve the system robustness performance against parameter variations and load disturbances. To handle the intrinsic chattering of SMC,an adaptive law and an extended state observer( ESO) are utilized in the speed SMC controller design. The adaptive law is used to estimate the internal parameter variations and compensate for the disturbances caused by model uncertainty. In addition,the ESO is introduced to estimate the load disturbance in real time. The estimated value is used as a feed-forward compensator for the speed adaptive sliding-mode controller to further increase the system's ability to resist disturbances. The proposed composite method,which combines adaptive SMC( ASMC) and ESO,is compared with PI control and ASMC. Both the simulation and experimental results demonstrate that the proposed method alleviates the chattering of SMC systems and improves the dynamic response and robustness of the speed control system against disturbances.

关键词： permanent-magnet synchronous motor(PMSM) adaptive sliding-mode control extended state observer speed control

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Comparison of outcomes between overlapping-spot and single-spot photodynamic therapy for circumscribed choroidal hemangioma

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International Journal of Ophthalmology(English edition) 2014年第1期7卷 66-70页

作者： Zhao-An Su Xia-jing Tang li-Xia Zhang Xiao-Hong Su Eye Center the Second Affiliated Hospital of Zhejiang University School of Medicine

AIM: To compare the efficacy and safety of photodynamic therapy(PDT) with overlapping multiple spots and single spot for treating circumscribed choroidal hemangioma. · METHODS: Twenty-two patients(22 eyes) with symptomatic circumscribed choroidal hemangioma received PDT treatment. Fourteen patients received overlapping spots(two to three spots) PDT,whereas eight patients received single-spot PDT. Laser was used at 50J/cm2for 83s in the overlapping-spot group and 50J/cm2for 166s in the single-spot group. Clinical examination,funduscopy,fluorescein angiography,and ultrasonography were performed at baseline and after treatment. ·RESULTS: The mean follow-up time was 28.5 ±8.0 months in the overlapping-spot group and 27.0 ±5.0 months in the single-spot group. Nine patients(64.2%) had their vision improved over two lines on the Snellen chart,and five patients showed stable visual acuity in the overlapping-spot group. The mean thickness of tumor decreased from 2.7 ±0.8mm to 1.2±0.9mm,and the mean greatest tumor linear dimension decreased from 7.4 ± 1.5mm to 4.5 ±3.5mm after treatment. In the single-spot group,two patients(25%) had their vision improved over two lines on the Snellen chart,and six patients had unchanged stable vision. The mean tumor thickness in this group decreased from 2.5±0.7mm to 1.4±1.0mm,and the mean greatest tumor linear dimension decreased from 7.2±1.3mm to 4.7±3.6mm. No significant differences in visual improvement and tumor regression were found between the two groups. ·CONCLUSION:Overlapping-spotPDTunderappropriate treatment parameters and strategies is as effective andsafe as single-spot PDT for treating symptomatic circumscribed choroidal hemangioma. Improved or stabilized visual acuity was achieved as a result of tumor regression.

关键词： photodynamic therapy circumscribed choroidal hemangioma spot pattern

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