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Granulocyte-colony stimulating factor therapy improves survival in patients with hepatitis B virus-associated acuteon-chronic liver failure

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World Journal of Gastroenterology 2013年第7期19卷 1104-1110页

作者： Xue-Zhang Duan Fang-Fang Liu jing-jing tong Hao-Zhen Yang jing Chen Xiao-Yan Liu Yuan-Li Mao Shao-Jie Xin Jin-Hua Hu Liver Failure Treatment and Research Center Beijing 302 Hospital Center for Clinical Laboratory Beijing 302 Hospital

AIM:To evaluate the safety and efficacy of granulocyte-colony stimulating factor(G-CSF) therapy in patients with hepatitis B virus(HBV)-associated acuteon-chronic liver failure(ACLF).METHODS:Fifty-five patients with HBV-associated ACLF were randomized into two groups:the treatment group and the control ***-seven patients in the treatment group received G-CSF(5 μg/kg per day,six doses) treatment plus standard therapy,and 28 patients in the control group received standard therapy *** peripheral CD34 + cell count was measured consecutively by flow *** white blood cell count,biochemical parameters,and other clinical data of these patients were recorded and *** patients were followed up for a period of 3 mo to evaluate the changes in liver function and survival ***:The peripheral neutrophil and CD34 + cell counts in the G-CSF group increased on day 3 from the onset of therapy,continued to rise on day 7,and remained elevated on day 15 compared to those of the control ***-Turcotte-Pugh score of patients in the treatment group was improved on day 30 from the onset of G-CSF therapy,compared to that in the controls(P = 0.041).Model for End-Stage of Liver Disease score of patients in the treatment group was improved on day 7(P = 0.004) and remained high on day 30 from the onset of G-CSF therapy(P < 0.001) compared to that in *** 3 mo of follow-up observation,the survival rate in the treatment group(48.1%) was significantly higher than that in the control group(21.4%)(P = 0.0181).CONCLUSION:G-CSF therapy promoted CD34 + cell mobilization in patients with HBV-associated ACLF,and improved the liver function and the survival rate of these patients.

关键词： Acute-on-chronic liver failure Granulocytecolony stimulating factor Hepatitis B virus

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Electrolyte induced synergistic construction of cathode electrolyte interphase and capture of reactive free radicals for safer high energy density lithium-ion battery

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Journal of Energy Chemistry 2023年第12期87卷 207-214,I0006页

作者： Mengfei Ding Xuning Feng Yong Peng jing jing tong Bowen Hou Yalan Xing Weifeng Zhang Li Wang Yu Wu Jiabin Lv Chunyan Luo Dejun Xiong Shichao Zhang Minggao Ouyang School of Materials Science and Engineering Beihang UniversityBeijing 100191China State Key Laboratory of Intelligent Green Vehicle and Mobility Tsinghua UniversityBeijing 100084China School of Vehicle and Mobility Tsinghua UniversityBeijing 100084China School of Mechanical and Electronic Control Engineering Beijing Jiaotong UniversityBeijing 100044China Institute of Nuclear and New Energy Technology Tsinghua UniversityBeijing 100084China School of Materials Science and Engineering Beijing Institute of TechnologyBeijing 100081China Farasis Energy(GanZhou)Co.Ltd GanZhou 341000JiangxiChina

As the energy density of battery increases rapidly,lithium-ion batteries(LIBs)are facing serious safety issue with thermal runaway,which largely limits the large-scale applications of high-energy-density *** is generally agreed that the chemical crosstalk between the cathode and anode leads to thermal runaway of ***,a multifunctional high safety electrolyte is designed with synergistic construction of cathode electrolyte interphase and capture of reactive free radicals to limit the intrinsic pathway of thermal *** cathode electrolyte interphase not only resists the gas attack from the anode but suppresses the parasitic side reactions induced by *** the function of free radical capture has the ability of reducing heat release from thermal runaway of *** dual strategy improves the intrinsic safety of battery prominently that the triggering temperature of thermal runaway is increased by 24.4℃and the maximum temperature is reduced by 177.7℃.Simultaneously,the thermal runaway propagation in module can be ***,the electrolyte design balances the trade-off of electrochemical and safety performance of high-energy *** capacity retention of LiNi_(0.8)Co_(0.1)Mn_(0.1)O_(2)|graphite pouch cell has been significantly increased from 53.85%to 97.05%with higher coulombic efficiency of 99.94%at operating voltage extended up to 4.5 V for 200 ***,this work suggests a feasible strategy to mitigate the safety risk of high-energy-density LIBs without sacrificing electrochemical performances.

关键词： Lithium-ion battery Electrolyte Battery safety Thermal runaway

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Characterization of glucose-6-phosphate dehydrogenase deficiency and identification of a novel haplotype 487G>A/IVS5-612(G>C) in the Achang population of southwestern China

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Science China(Life Sciences) 2007年第4期50卷 479-485页

作者： YANG YinFeng, ZHU YueChun, LI DanYi, LI ZhiGang, Lü HuiRu, WU jing, TANG jing & tong ShuFen Department of Biochemistry, Faculty of Basic Medicine, Kunming University of Medical Sciences, Kunming 650031, China These authors contributed equally to this work 1. Department of Biochemistry Faculty of Basic Medicine Kunming University of Medical Sciences Kunming 650031 China

The prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency and its gene mutations were studied in the Achang population from Lianghe County in Southwestern China. We found that 7.31% (19 of 260) males and 4.35% (10 of 230) females had G6PD deficiency. The molecular analysis of G6PD gene exons 2―13 was performed by a PCR-DHPLC-Sequencing or PCR-Sequencing. Sixteen inde-pendent subjects with G6PD Mahidol (487G>A) and the new polymorphism IVS5-612 (G>C), which combined into a novel haplotype, were identified accounting for 84.2% (16/19). And 100% Achang G6PD Mahidol were linked to the IVS5-612 C. The percentage of G6PD Mahidol in the Achang group is close to that in the Myanmar population (91.3% 73/80), which implies that there are some gene flows between Achang and Myanmar populations. Interestingly, G6PD Canton (1376G>T) and G6PD Kaiping (1388G>A), which were the most common G6PD variants from other ethnic groups in China, were not found in this Achang group, suggesting that there are different G6PD mutation profiles in the Achang group and other ethnic groups in China. Our findings appear to be the first documented report on the G6PD genetics of the AChang people, which will provide important clues to the Achang ethnic group origin and will help prevention and treatment of malaria in this area.

关键词： glucose-6-phosphate dehydrogenase deficiency Achang population G6PD Mahidol gene mutation Myanmar population

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Predictive value of the Chinese group on the study of severe hepatitis B-acute-on-chronic liver failure score in the short-term prognosis of patients with hepatitis B virus-related acute-on-chronic liver failure

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Chinese Medical Journal 2019年第13期132卷 1541-1549页

作者： jing-jing tong Wei Zhao Xiu-Ying Mu Xiang Xu Hai-Bin Su Xiao-Yan Liu jing Chen Xing-Ran Zhai Yu Wang Jin-Hua Hu Liver Failure Treatment and Research Center The Fifth Medical Center of Chinese PLA General HospitalBeijing 100039China Department of Clinical Laboratory China-Japan Friendship HospitalBeijing 100029China Peking University 302 Clinical Medical School Beijing 100039China Medical School of Chinese PLA Beijing 100853China

Background:As a large,prospective,multicenter study-based prognostic score for hepatitis B virus-related acute-on-chronic liver failure(HBV-ACLF),the Chinese group on the study of severe hepatitis B-acute-on-chronic liver failure score(COSSH-ACLFs),has been approved by some foreign scholars;however,its predictive value needs to be *** study investigated the predictive value of COSSH-ACLFs for short-term prognosis in Chinese patients with ***:This retrospective cohort study included 751 patients with HBV-ACLF admitted to the Fifth Medical Center of Chinese PLA General Hospital between January 2011 and December *** method was used to assess the correlation of COSSHACLFs with classical *** COX multivariate regression models were used to confirm the relationship between COSSHACLFs and short-term prognosis in patients with HBV-ACLF,and stratified analysis was used to further verify the stability of this *** compared the predictive powers of COSSH-ACLFs and other classical scores using area under the receiver operating characteristic curve(AUROC)and ***:A total of 975 patients with HBV-ACLF were screened,and 751 were analyzed(623 male and 128 female).COSSH-ACLFs was the highest in patients with end-stage ACLF,followed by those with middle-and early-stage ACLF(H=211.8,P<0.001).In the fully adjusted model,COX multivariate regression analysis revealed that COSSH-ACLFs(as a continuous variable)was independently and positively correlated with mortality risk in patients with HBV-ACLF at 28 days(hazard ratio[HR]:1.37[1.22,1.53],P<0.001)and 90 days(HR:1.43[1.29,1.58],P<0.001).The same trend could be observed in the crude model and minimally adjusted *** AUROCs of COSSH-ACLFs for 28-day and 90-day prognoses in patients with HBV-ACLF were 0.807 and 0.792,respectively,indicating a stronger predictive accuracy than those of classic ***:COSSH-ACLFs,with a superior predictive accuracy compared wit

关键词： Hepatitis B virus-related acute-on-chronic liver failure COSSH-ACLF score Predictive value Prognosis

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Quantitative proteomics analysis reveals the pathogenesis of obstructed defecation syndrome caused by abnormal expression of dystrophin

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World Journal of Gastroenterology 2024年第45期30卷 4817-4835页

作者： Wen-Zhe Li Yu Xiong Tian-Kun Wang Yan-Yan Chen Song-Lin Wan Lu-Yao Li Meng Xu jing-jing tong Qun Qian Cong-Qing Jiang Wei-Cheng Liu Department of Colorectal and Anal Surgery(Clinical Center for Pelvic Floor Surgery) Clinical Center of Constipation and Pelvic Floor Disease of WuhanHubei Key Laboratory of Intestinal and Colorectal DiseasesClinical Center of Intestinal and Colorectal Diseases of Hubei ProvinceQuality Control Center of Colorectal and Anal Surgery of Health Commission of Hubei ProvinceZhongnan Hospital of Wuhan UniversityWuhan 430071Hubei ProvinceChina Department of Radiation and Medical Oncology for Esophageal Mediastinal and Lymphatic Tumors Zhongnan Hospital of Wuhan UniversityWuhan 430071Hubei ProvinceChina Center for Evidence-Based and Translational Medicine Zhongnan Hospital of Wuhan UniversityWuhan 430071Hubei ProvinceChina Department of Pathology Zhongnan Hospital of Wuhan UniversityWuhan 430071Hubei ProvinceChina School of Life Sciences Central China Normal UniversityWuhan 430071Hubei ProvinceChina

BACKGROUND Obstructed defecation syndrome(ODS)represents the most prevalent form of chronic constipation,affecting a diverse patient population,leading to numerous complications,and imposing a significant burden on healthcare *** ODS patients have insufficient rectal propulsion,but the exact mechanism underlying the pathogenesis of ODS remains *** To explore the molecular mechanism underlying the pathogenesis of *** A total of 30 pairs of rectal samples were collected from patients with ODS(ODS group)or grade IV prolapsed hemorrhoids without constipation(control group)for quantitative proteomic and bioinformatic ***,50 pairs of paraffin-embedded rectal specimens were selected for immunohistochemistry and immunofluorescence studies to validate the analysis *** intestinal smooth cell contractile function experiments and electrophysiological experiments were conducted to verify the physiological functions of target *** ultrastructure was detected using transmission electron *** In comparison to the control group,the expression level of dystrophin(DMD)in rectal specimens from ODS patients was markedly *** finding was corroborated using immunohistochemistry and immunofluorescence *** diminished expression of DMD compromised the contractile function of intestinal smooth muscle *** the molecular level,nucleoporin protein 153 and L-type voltage-gated calcium channel were found to be overexpressed in intestinal smooth muscle cells exhibiting downregulated DMD *** experiments confirmed an excessive influx of calcium ions into these ***,vacuolar-like structures which may be associated with excessive calcium influx were observed in the cells by transmission electron *** Decreased DMD expression in intestinal smooth muscle may upregulate L-type voltage-gated calcium channel expression,leading to excessive calcium in

关键词： Obstructed defecation syndrome Dystrophin Smooth muscle Contraction Proteomics L-type voltage-gated calcium channel

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新疆南天山榆树沟含石榴夕线石泥质麻粒岩的超高温变质作用及其构造意义

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岩石学报 2025年第1期41卷 215-236页

作者：荆彦清仝来喜刘兆李超大陆动力学国家重点实验室西北大学地质学系西安710069

天山造山带是中亚造山带的重要组成部分,新疆南天山造山带出露有橄榄岩-麻粒岩地体,其北部榆树沟地区麻粒岩超高温变质作用的确定及P-T-t轨迹的建立为南天山造山带增生造山过程的研究提供了重要依据。本文主要以榆树沟地区3个含石榴夕... 详细信息

天山造山带是中亚造山带的重要组成部分,新疆南天山造山带出露有橄榄岩-麻粒岩地体,其北部榆树沟地区麻粒岩超高温变质作用的确定及P-T-t轨迹的建立为南天山造山带增生造山过程的研究提供了重要依据。本文主要以榆树沟地区3个含石榴夕线石泥质麻粒岩为研究对象,在详细岩相学研究的基础上进行了温压计算、相平衡模拟及锆石U-Pb年龄分析。结果显示其普遍保留了4个阶段的矿物组合:(1)峰前阶段(M1)主要是石榴子石核部与核部包裹体矿物Grt1+Bt1+Pl1+Ilm1+Rt1+Qz1±Ksp1;(2)峰期阶段(M2)由石榴子石幔部和基质矿物核部组成,Grt2+Sil2+Ksp2+Pl2+Ilm2+Rt2+Qz2+Liq2,其温压条件为910~973℃和0.90~1.09GPa;(3)高压麻粒岩相阶段(M3)主要是石榴子石内边部和边部包裹体及基质中矿物的边部,Grt3+Bt3+Sil3+Ksp3+Pl3+Ilm3+Rt3+Qz3+Liq3,其温压结果为845~869℃和1.01~1.14GPa;(4)角闪岩相退变质阶段(M4)以石榴子石的外边部及围绕石榴子石的矿物为代表,Grt4+Bt4+Sil4+Ksp4+Pl4+Ilm4+Qz4±Rt4,其温压条件为701~737℃和0.7~0.8GPa。温压结果指示泥质麻粒岩记录了一条逆时针P-T轨迹。锆石U-Pb年龄分析结果指示原岩沉积是在427Ma之后,超高温变质作用发生在397~409Ma,压力峰期变质作用年龄范围为381~395Ma,退变质年龄结果为355~387Ma。超高温变质作用的热源可能是泥盆纪弧岩浆侵入,随后麻粒岩及与之接触的橄榄岩被俯冲板片捕获并经历了高压变质作用,后期受挤压折返发生了退变质降压冷却。

关键词：泥质麻粒岩超高温变质作用 P-T轨迹榆树沟南天山

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伪狂犬病病毒变异株gE蛋白单克隆抗体的制备与鉴定

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中国动物传染病学报 2017年第3期25卷 23-27页

作者：武吉强徐晶晶童武王涛叶超郑浩单同领童光志李国新中国农业科学院上海兽医研究所上海200241 江苏高校动物重要疫病与人兽共患病防控协同创新中心扬州225009

为制备伪狂犬病病毒变异株gE蛋白的单克隆抗体,PCR扩增伪狂犬病病毒gE蛋白主要抗原表位区的基因片段,并将其克隆至原核表达载体p Cold-TF。经1 mmol/L IPTG低温诱导,表达了约90 k Da的gE融合蛋白。融合蛋白用镍离子金属亲和层析柱纯化后... 详细信息

为制备伪狂犬病病毒变异株gE蛋白的单克隆抗体,PCR扩增伪狂犬病病毒gE蛋白主要抗原表位区的基因片段,并将其克隆至原核表达载体p Cold-TF。经1 mmol/L IPTG低温诱导,表达了约90 k Da的gE融合蛋白。融合蛋白用镍离子金属亲和层析柱纯化后,免疫6周龄BALB/c雌性小鼠,经细胞融合和筛选,获得了2株稳定分泌抗gE蛋白单克隆抗体的杂交瘤细胞(5B2和6E6)。间接免疫荧光试验(indirect immunofl uorescence assay,IFA)显示2株单克隆抗体均能与PRV变异株(JS-2012)反应,但与gE缺失的疫苗毒Bartha-K61株无反应。Western blot结果显示5B2和6E6与gE蛋白不反应,提示2株单克隆抗体针对的可能是gE蛋白的构象表位。本研究获得的gE蛋白单克隆抗体为伪狂犬病病毒变异株的鉴定与功能研究以及野毒株和疫苗株的鉴别诊断提供了良好的工具。

关键词：伪狂犬病毒变异株 gE蛋白单克隆抗体

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Association between autoimmune gastritis and gastric polyps:Clinical characteristics and risk factors

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 73-87页

作者： jing-Zheng Jin Xiao Liang Shu-Peng Liu Rui-Lan Wang Qing-Wei Zhang Yu-Feng Shen Xiao-Bo Li Division of Gastroenterology and Hepatology Renji HospitalShanghai Jiao Tong University School of MedicineShanghai 200127China Division of Gastroenterology and Hepatology Shanghai Institute of Digestive DiseasesShanghai 200001China Division of Gastroenterology and Hepatology Renji Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200127China Division of Gastroenterology and Hepatology Sichuan Armed Police Corps HospitalLeshan 610041Sichuan ProvinceChina Division of Gastroenterology and Hepatology NHC Key Laboratory of Digestive DiseasesRenji HospitalShanghai Jiao Tong University School of MedicineShanghai 200127China Department of Gastroenterology Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai Institute of Digestive DiseasesShanghai 200127China

BACKGROUND The relationship between autoimmune gastritis(AIG)and gastric polyps(GPs)is not well *** To explore the clinical characteristics and risk factors of AIG with GPs in *** This double center retrospective study included 530 patients diagnosed with AIG from July 2019 to July *** collected clinical,biochemical,serological,and demographic data were of each *** regression analyses,both multivariate and univariate,were conducted to pinpoint independent risk factors for GPs in patients with AIG *** operating characteristic curves were utilized to establish the optimal cutoff values,sensitivity,and specificity of these risk factors for predicting GPs in patients with *** Patients with GPs had a higher median age than those without GPs[61(52.25-69)years vs 58(47-66)years,P=0.006].The gastrin-17 levels were significantly elevated in patients with GPs compared with those without GPs[91.9(34.2-138.9)pmol/mL vs 60.9(12.6-98.4)pmol/mL,P<0.001].Additionally,the positive rate of parietal cell antibody(PCA)antibody was higher in these patients than in those without GPs(88.6%vs 73.6%,P<0.001).Multivariate and univariate analyses revealed that PCA positivity[odds ratio(OR)=2.003,P=0.017],pepsinogen II(OR=1.053,P=0.015),and enterochromaffin like cells hyperplasia(OR=3.116,P<0.001)were significant risk factors for GPs,while pepsinogen I was identified as a protective *** PCA positivity and enterochromaffin like cells hyperplasia are significant risk factor for the development of GPs in patients with *** gastrin-17 levels may also play a role in this *** findings suggest potential targets for further research and therapeutic intervention in managing GPs in patients with AIG.

关键词： Autoimmune gastritis Gastric polyps Neuroendocrine tumor Risk factors Nomogram

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广西成年居民糖尿病患病率和知晓、治疗及控制情况调查

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中国慢性病预防与控制 2018年第4期26卷 250-253,261页

作者：陆伟江杨虹蒙晓宇罗水英许晶晶黄佟黄颖广西壮族自治区疾病预防控制中心慢性病防制所广西南宁530028

目的了解广西18岁及以上成年居民糖尿病患病、知晓、治疗和控制情况及流行特点,为广西开展糖尿病防治,制定相关卫生政策提供数据支持和科学依据。方法数据来源于2010年广西慢性病及其危险因素监测专项调查资料。对3 599名18岁及以上居... 详细信息

目的了解广西18岁及以上成年居民糖尿病患病、知晓、治疗和控制情况及流行特点,为广西开展糖尿病防治,制定相关卫生政策提供数据支持和科学依据。方法数据来源于2010年广西慢性病及其危险因素监测专项调查资料。对3 599名18岁及以上居民进行问卷调查和实验室检测,分析糖尿病患病率、知晓率、治疗率与控制率。用SPSS 17.0软件对数据进行统计学分析,率的比较采用χ~2检验。结果广西18岁及以上居民糖尿病粗患病率为8.0%,加权调整后患病率为7.1%。城市居民糖尿病患病率(11.5%)高于农村(5.9%),男性(8.4%)高于女性(5.8%),差异均有统计学意义(χ~2分别为28.681、9.588,P<0.01);18~44岁、45~59岁、60岁及以上年龄组糖尿病患病率分别为3.4%、9.4%和17.9%,随年龄的增长呈上升趋势,差异有统计学意义(χ~2=150.290,P<0.01)。糖尿病患者的知晓率、治疗率和控制率分别为21.4%、20.2%和23.1%。城市居民知晓率与治疗率(分别为39.8%、38.2%)均高于农村居民(分别为11.8%、10.8%),差异均有统计学意义(χ~2分别为26.853、26.975,P<0.01)。18~44岁、45~59岁、60岁及以上年龄组知晓率分别为8.2%、22.9%和29.7%,治疗率分别为6.9%、22.9%和27.3%,均随着年龄的增长逐渐升高,差异均有统计学意义(χ~2分别为11.789、11.342,P<0.01)。结论广西糖尿病防治工作应重点应面向中老年人群,加强对45岁以上居民的糖尿病筛查,加强对农村以及低文化层次人群的糖尿病健康教育,加强及完善糖尿病社区综合管理,采取有针对性的干预措施来防控糖尿病的发生和发展。

关键词：糖尿病患病率知晓率治疗率控制率

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The Periodic Solutions of a Nonhomogeneous String with Dirichlet-Neumann Condition

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Chinese Annals of Mathematics,Series B 2014年第4期35卷 619-632页

作者： Changqing tong jing ZHENG Department of Mathematics Hangzhou Dianzi University

This paper deals with the existence of periodic solutions of a nonhomogeneous string with Dirichlet-Neumann condition. The authors consider the case that the period is irrational multiple of space length and prove that for some irrational number, zero is not the accumulation point of the spectrum of the associated linear operator. This result can be used to prove the existence of the periodic solution avoid using Nash-Moser iteration.

关键词： Nonhomogeneous string Periodic solutions Weak solution Continuedfraction Lagrange constant

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