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Posterior chamber phakic intraocular lens for the correction of high myopic anisometropic amblyopia in adults

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International Journal of Ophthalmology(English edition) 2018年第11期11卷 1870-1874页

作者： jing Zhang jing zhuang Ke-Ming Yu Shenzhen Eye Hospital Shenzhen Key Laboratory of Ophthalmology Affiliated Shenzhen Eye Hospital of Jinan University State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center Sun Yat-sen University

This study aims to evaluate the clinical results of posterior chamber implantable collamer lens（ICL） implantation in adults with high myopic anisometropic amblyopia. Thirteen patients aged 27.04±7.24 y（range 19.2 to 42.5） were studied. ICL implantation was performed in 13 amblyopic eyes. The mean myopic anisometropia was significantly dropped from-10.70±3.02 D preoperatively to ＋0.09±1.67 D at 1 mo postoperatively. The logMAR CDVA at 3 d, 1, 3 and mean 9 mo postoperatively improved by a mean of 1.69, 2.50, 3.01 and 3.00 lines and gained more than 2 lines accounted for 23.08%（3 eyes）, 41.67%（5 eyes）, 63.63%（7 eyes） and 55.56%（5 eyes）, respectively. The contrast sensitivity of amblyopic eyes was significantly increased after surgery. Four patients partially recovered near stereopsis（400＂ to 100＂）. There was no severe complications were observed. ICL implantation alone can improve vision, contrast sensitivity, and partial restoration of binocular vision in adult patients with high myopic anisometropia.

关键词： phakic intraocular lens myopic anisometropic amblyopia refractive surgery

Glycogen synthase kinase-3β inhibitor SB216763 promotes DNA repair in ischemic retinal neurons

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Neural Regeneration Research 2021年第2期16卷 394-400页

作者： Zhang, jing Zhi-Peng, Lai Chen, Pei Yang, Ying zhuang, jing Ke-Ming, Yu State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou Guangdong Province

Glycogen synthase kinase-3β(GSK-3β) has been shown to attenuate DNA damage in nerve cells, thereby enhancing neuronal survival under pathological conditions;however, the underlying mechanism remains unclear. An in vitro serum-starvation retinal neuron model and in vivo ischemia/reperfusion retina injury rat model were established and treated with SB216763, a GSK-3β inhibitor. SB21673 decreased the formation of γ-H2 A histone family member X foci and enhanced the viability of ischemic retinal neurons. In addition, SB216763 upregulated expression of phosphorylated-CREB1, a ligase IV transcription factor, and significantly increased the transcriptional activity of ligase IV in ischemic retinal neurons. These results were confirmed in rat retinas following ischemia/reperfusion injury. Furthermore, we found that unlike lithium chlorine(a well-known direct inhibitor of GSK-3β), SB216763 inhibited GSK-3β activity by suppressing its phosphorylation. Taken together, our results suggest that GSK-3β inhibition enhances repair of DNA double-strand breaks by upregulating ligase IV expression in ischemic retinal neurons. This study was approved by the Institutional Animal Care and Use Committee of Zhongshan Ophthalmic Center on February 18, 2018.

关键词： factor GSK-3β in vitro injury ligase IV neuroprotection optic nerve pathways protein repair

A Few Specialized Issues That Should Be Focused on Anti-HIV Drug Evaluation In Vitro

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Virologica Sinica 2010年第4期25卷 301-306页

作者： Dao-min zhuang jing-yun LI State Key Laboratory of Pathogen and Biosecurity Institute of Microbiology and Epidemiology Academy of Military Medical Science Beijing 100071 China

Since the introduction of antiretroviral therapy (ART), the lifespan and quality of life of patients infected with HIV have been significantly improved. But treatment efficacy was compromised eventually by the development of resistance to antiretroviral drugs, and more new anti-HIV drugs with lower toxicity and higher activity were needed. Based on the experience and lessons learned from the treatment in the developed countries, US FDA suggested that more pharmacodynamical researches should be considered ahead of the clinical trials. To facilitate the anti-HIV drug research and development, we reviewed a few specialized issues that should be focused on drug evaluations in vitro, including: 1) Mechanism of action studies, demonstrating the candidate drug's efficacy to specifically inhibit viral replication or a virus-specific function and confirm the drug target. 2) Drug resistance studies, selecting the drug-resistant variants in vitro and determining the activities inhibiting HIV isolates resistant to approved antiretroviral drugs of the same class. 3) Antiviral activity in vitro in the presence of serum proteins, ascertaining whether an investigational product is significantly bound by serum proteins. 4) Combination activity analysis, evaluating in vitro antiviral activity of an investigational product in two-drug combinations with other drugs approved.

关键词： Human immunodeficiency virus Drug evaluation Drug resistance

Comparison of anterior section parameters using anterior segment optical coherence tomography and ultrasound biomicroscopy in myopic patients after ICL implantation

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International Journal of Ophthalmology(English edition) 2016年第1期9卷 58-62页

作者： jing Zhang Hui-Hui Luo jing zhuang Ke-Ming Yu State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center Sun Yat-sen University

AIM：To compare the agreement of anterior chamber depth（ACD）and central vault measurements obtained by anterior segment optical coherence tomography（AS-OCT）and ultrasound biomicroscopy（UBM）of post surgical high myopic eyes with posterior chamber phakic intraocular lens（Visian ICL;STAAR Surgical）implantation.·METHODS：Fifty-two phakic eyes of 28 high myopic patients who underwent implantable Collamer lens（ICL）surgery for the correction of high myopia were *** postoperative ACD,the distance between the corneal endothelium and the anterior surface of ICL（cornea-ICL）and the central vault were measured with the AS-OCT system and the UBM *** correlation coefficient（ICC）and the Bland-Altman plot were used to evaluate the repeatability and agreement of two devices.·R ESULTS：The mean ACD,cornea-ICL and central vault in the 52 phakic eyes after ICL surgery was 3.19±0.28 mm,2.47±0.28 mm,0.50±0.19 mm by AS-OCT and3.13±0.25 mm,2.49±0.25 mm,0.44±0.19 mm by UBM,*** comparison of ACD and central vault measurements showed significant differences between AS-OCT and UBM（〈0.05）.However,no statistically significant difference was found between these imaging techniques in cornea-ICL（〉0.05）.The Pearson correlation coefficient（）between AS-OCT and UBM measurements for ACD,cornea-ICL and vault was0.88,0.80 and 0.89,respectively（〈0.001）.The ICC was0.89-0.94 for the measurements of AS-OCT and ***-Altman analysis showed the 95%limits of agreement of ACD,cornea-ICL,central vault measurements between these two devices were-0.20 to 0.32 mm,-0.36to 0.32 mm and-0.12 to 0.24 mm,respectively.58·CONCLUSION：Central ACD and vault measurements using AS-OCT demonstrated a slight significantly higher value than using UBM in phakic eyes after ICL *** two devices should not be used interchangeably for measurements of central ACD and vault in patients after phakic intraocular lens implantation.

关键词： anterior chamber depth vault phakicintraocular lens anterior segment imaging optical coherencetomography ultrasound biomicroscopy

Rapid odor perception in rat olfactory bulb by microelectrode array

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第12期13卷 1015-1023页

作者： Jun ZHOU Qi DONG Liu-jing zhuang Rong LI Ping WANG Biosensor National Special Lab Key Lab for Biomedical Engineering of Ministry of EducationDepartment of Biomedical EngineeringZhejiang UniversityHangzhou 310027China

Responses of 302 mitral/tufted (M/T) cells in the olfactory bulb were recorded from 42 anesthetized freely breathing rats using a 16-channel microwire electrode *** vapors of four pure chemicals,anisole,carvone,citral and isoamyl acetate were *** aligning spike trains to the initial phase of the inhalation after odor onset,the responses of M/T cells showed transient temporal features including excitatory and inhibitory *** odor-evoked patterns indicated that mammals recognize odors within a short respiration cycle after odor *** to the small amount of information received from a single cell,we pooled results from all responsive M/T cells to study the ensemble *** firing rates of the cell ensembles were computed over 100 ms bins and population vectors were *** high dimension vectors were condensed into three dimensions for visualization using principal component *** trajectories of both excitatory and inhibitory cell ensembles displayed strong dynamics during odor *** distances among cluster centers were enlarged compared to those of the resting ***,we presumed that pictures of odor information sent to higher brain regions were depicted and odor discrimination was completed within the first breathing cycle.

关键词： Rapid odor perception Mitral/tufted cell Multielectrode array Odor trajectory

Advances in single-cell sequencing technology in microbiome research

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Genes & Diseases 2024年第4期11卷 201-219页

作者： Yinhang Wu jing zhuang Yifei Song Xinyi Gao Jian Chu Shuwen Han Huzhou Central Hospital Affiliated Central Hospital Huzhou UniversityHuzhouZhejiang 313000China Key Laboratory of Multiomics Research and Clinical Transformation of Digestive Cancer of Huzhou HuzhouZhejiang 313000China The Fifth Affiliated Clinical Medical College of Zhejiang Chinese Medical University HangzhouZhejiang 313000China Zhejiang Provincial People's Hospital and Affiliated People's Hospital Hangzhou Medical CollegeHangzhouZhejiang 310000China

With the rapid development of histological techniques and the widespread applica-tion of single-cell sequencing in eukaryotes,researchers desire to explore individual *** and functional expression,which deepens our understanding of *** this review,the history of the development of microbial detection technologies was revealed and the difficulties in the application of single-cell sequencing in microorganisms were dissected as ***,the characteristics of the currently emerging microbial single-cell sequencing(Microbe-seq)technology were summarized,and the prospects of the application of Microbe-seq in microorganisms were distilled based on the current development *** its mature development,the Microbe-seq technology was still in the optimization stage.A retrospective study was conducted,aiming to promote the widespread application of single-cell sequencing in microorganisms and facilitate further improvement in the technol-ogy.

关键词： Bacterialantibiotic resistance Host immunity Host-phage interaction Microbial single-cell sequencing Microorganisms Single cell Single-cell sequencing technology

Integrative transcriptome,proteome,and microRNA analysis reveals the effects of nitrogen sufficiency and deficiency conditions on theanine metabolism in the tea plant(Camellia sinensis)

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Horticulture Research 2020年第1期7卷 1865-1877页

作者： Zhi-Wei Liu Hui Li Jie-Xia Liu Yu Wang jing zhuang Tea Science Research Institute Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East ChinaCollege of HorticultureNanjing Agricultural University210095 NanjingChina State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University210095 NanjingChina

Nitrogen(N)is associated with amino acid metabolism in higher *** is an important amino acid in tea *** explore the relationship between theanine metabolism and N conditions,we examined the differentially expressed genes(DEGs),proteins(DEPs),and microRNAs(DEMs)involved in theanine metabolism in tea plant shoots and roots under N sufficiency and deficiency ***,proteome,and microRNA analyses were performed on tea plant shoots and roots under N sufficiency and deficiency *** contents of theanine,expression levels of genes involved in theanine metabolism,contents of proteinogenic amino acids,and activity of enzymes were *** DEP–DEG correlation pairs and negative DEM–DEG interactions related to theanine metabolism were identified based on correlation *** expression profiles of DEGs and negative DEM–DEG pairs related to theanine biosynthesis were consistent with the sequencing *** results suggest that the molecular and physiological mechanism of theanine accumulation is significantly affected by N sufficiency and deficiency *** DEGs,DEPs,and DEMs and the activity of the enzymes involved in theanine biosynthesis might play vital roles in theanine accumulation under N sufficiency and deficiency conditions in the shoots and roots of tea plants.

关键词： metabolism sinensis ani

An Efficient Trap Passivator for Perovskite Solar Cells:Poly(propylene glycol)bis(2‑aminopropyl ether)

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Nano-Micro Letters 2020年第12期12卷 251-263页

作者： Ningli Chen Xiaohui Yi jing zhuang Yuanzhi Wei Yanyan Zhang Fuyi Wang Shaokui Cao Cheng Li Jizheng Wang CAS Key Laboratory of Organic Solids Beijing National Laboratory for Molecular SciencesInstitute of Chemistry Chinese Academy of SciencesBeijing 100190People’s Republic of China University of Chinese Academy of Sciences Beijing 100190People’s Republic of China Department of Physics Xiamen UniversityXiamen 361005People’s Republic of China CAS Key Laboratory of Analytical Chemistry for Living Biosystems Beijing National Laboratory for Molecular SciencesInstitute of Chemistry Chinese Academy of SciencesBeijing 100190People’s Republic of China School of Materials Science and Engineering Zhengzhou UniversityZhengzhou 450000People’s Republic of China

Perovskite solar cells(PSCs)are regarded as promising candidates for future renewable energy ***-density defects in the perovskite films,however,lead to unsatisfactory device ***,poly(propylene glycol)bis(2-aminopropyl ether)(PEA)additive is utilized to passivate the trap states in *** PEA molecules chemically interact with lead ions in perovskite,considerably passivate surface and bulk defects,which is in favor of charge transfer and ***,the PEA additive can efficiently block moisture and oxygen to prolong the device *** a result,PEA-treated MAPbI3(MA:CH3NH3)solar cells show increased power conversion efficiency(PCE)(from 17.18 to 18.87%)and good longterm *** PEA is introduced to(FAPbI3)1-x(MAPbBr3)x(FA:HC(NH2)2)solar cells,the PCE is enhanced from 19.66 to 21.60%.For both perovskites,their severe device hysteresis is efficiently relieved by PEA.

关键词： Defects Grain boundaries Passivation Stability Perovskite solar cells

Immobilization of chiral Rh catalyst on glass and application to asymmetric transfer hydrogenation of aryl ketones in aqueous media

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Chinese Chemical Letters 2014年第4期25卷 613-616页

作者： Tan-Yu Cheng jing-Lan zhuang Hui Yao Huai-Sheng Zhang Guo-Hua Liu Key Laboratory of Resource Chemistry of Ministry of Education College of Life and Environmental ScienceShanghai Normal University

A chiral catalyst, Cp＊RhTsDPEN （Cp＊ = pentamethyl cyclopentadiene, TsDPEN = substitutive phenylsulfonyl-l,2-diphenylethylenediamine）, was synthesized and immobilized at the surface of glass. The immobilized catalyst exhibited good catalytic efficiency for asymmetric transfer hydrogenation of aromatic ketones in water with HCOONa as hydrogen source.

关键词： Heterogeneous Rh catalyst Asymmetric transfer hydrogenation Aromatic ketones Glass suhstrate