检索结果-南通市图书馆

Characterization of inthomycin biosynthetic gene cluster revealing new insights into carboxamide formation

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2020年第9期18卷 677-683页

作者： WANG yong-Jiang WANG li HE xin XU dong-dong tang Jun MA Ya-Tuan YAN Yi-Jun GU yu-Cheng YANG jing HUANG Sheng-Xiong State Key Laboratory of Phytochemistry and P/ant Resources in West China and CAS Center for Excellence in Molecular Plant SciencesKunming Institute of BotanyChinese Academy of SciencesKunming 650201.China College of Chemistry and Pharmacy Norhwest Agriculture and Forestry UniversityYangling 712100.China Savaid Medical School University of Chinese Academy of ScienrcesBejing 100049.China Syngenta Jelott's Hill International Research Centre BracknellBerkshire RG426EYU.K.

Inthomycins are polyketide antibiotics which contain a terminal carboxamide group and a triene chain. Inthomycin B(1) and its two new analogues 2 and 3 were isolated from the crude extract of Streptomyces pactum L8. Identification of the gene cluster for inthomycin biosynthesis as well as the ^(15)N-labeled glycine incorporation into inthomycins are described. Combined with the gene deletion of the rare P450 domain in the NRPS module, a formation mechanism of carboxamide moiety in inthomycins was proposed via an oxidative release of the assembly chain assisted by the P450 domain.

关键词： Inthomycins Biosynthesis Gene cluster Carboxamide P450

Identification of Animal Rabies in Inner Mongolia and Analysis of the Etiologic Characteristics

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2014年第1期27卷 35-44页

作者： YIN jing Feng WANG Jin ling tang Qing DING yu lin TAO XiaoYan li Hao song Miao GUO ZhenYang SHEN xin xin liANG Guo dong WANG Feng Long College of Veterinary Medicine Inner Mongolia Agricultural University Key Laboratory for Medical Virology Ministry of HealthInstitute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention (IVDCChina CDC) State Key Laboratory for Infectious Disease Prevention and Control Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention (IVDCChina CDC)

Objective To perform pathological observation and etiological identification of specimens collected from dairy cows, beef cattle and dogs which were suspected of rabies in Inner Mongolia in 2012, and analyze their etiological characteristics. Methods Pathological observation was conducted on the brain specimens of three infected animals with Hematoxylin-Eosin staining, followed by confirmation using immunofluorescence and nested RT-PCR methods. Finally, phylogenetic analysis was conducted using the virus N gene sequence amplified from three specimens. Results Eosinophilic and cytoplasmic inclusion bodies were seen in neuronal cells of the CNS, and rabies non-characteristic histopathological changes were also detected in the CNS. The three brain specimens were detected positive. N gene nucleotide sequence of these three isolates showed distinct sequence identity, therefore they fell into different groups in the phylogenetic analysis. N gene in the cow and dog had higher homology with that in Hebei isolate, but that in the beef cattle had higher homology with that in Mongolian lupine isolate and Russian red fox isolate. Conclusion Rabies were observed in the dairy cow, beef cattle and canine in the farm in Inner Mongolia, in 2012, which led to a different etiologic characteristics of the epidemic situation.

关键词： Rabies Cow Histopathology Phylogeny

Catheter ablation for atrial fibrillation is associated with reduced risk of mortality in the elderly:a prospective cohort study and propensity score analysis

在线全文

学校读者我要写书评

暂无评论

Journal of Geriatric Cardiology 2020年第12期17卷 740-749页

作者： xin SU xin DU Shang-xin LU Chao JIANG jing DU Shi-Jun XIA Zhao-Jie dong Zhao-Xu JIA De-yong LONG Cai-Hua SANG Ri-Bo tang Nian liU song-Nan li Rong BAI Jian-Zeng dong Chang-Sheng MA Department of Cardiology Beijing Anzhen HospitalCapital Medical UniversityNational Clinical Research Centre for Cardiovascular DiseasesBeijingChina Heart Health Research Center BeijingChina The George Institute for Global Health Faculty of MedicineUniversity of New South WalesSydneyAustralia Beijing Centre for Disease Prevention and Control BeijingChina

Background It is unclear whether catheter ablation(CA)for atrial fibrillation(AF)affects the long-term prognosis in the *** study aims to evaluate the relationship between CA and long-term outcomes in elderly patients with *** Patients more than 75 years old with non-valvular AF were prospectively enrolled between August 2011 and December 2017 in the Chinese Atrial Fibrillation Registry *** who underwent CA at baseline were propensity score matched(1:1)with those who did not receive *** outcome events included all-cause mortality,cardiovascular mortality,stroke/transient ischemic attack(TIA),and cardiovascular *** Overall,this cohort included 571 ablated patients and 571 non-ablated patients with similar characteristics on 18 *** a mean follow-up of 39.75±19.98 months(minimum six months),24 patients died in the ablation group,compared with 60 deaths in the non-ablation group[hazard ratio(HR)=0.49,95%confidence interval(CI):0.30-0.79,P=0.0024].Besides,6 ablated and 29 non-ablated subjects died of cardiovascular disease(HR=0.25,95%CI:0.11-0.61,P=0.0022).A total of 27 ablated and 40 non-ablated patients suffered stroke/TIA(HR=0.79,95%CI:0.48-1.28,P=0.3431).In addition,140 ablated and 194 non-ablated participants suffered cardiovascular hospitalization(HR=0.84,95%CI:0.67-1.04,P=0.1084).Subgroup analyses according to gender,type of AF,time since onset of AF,and anticoagulants exposure in initiation did not show significant *** In elderly patients with AF,CA may be associated with a lower incidence of all-cause and cardiovascular mortality.

关键词： Atrial fibrillation Catheter ablation Mortality Stroke The elderly

Cover a 3-regular Claw-free Graph by Induced Matchings

在线全文

学校读者我要写书评

暂无评论

Chinese Quarterly Journal of Mathematics 2011年第3期26卷 355-359页

作者： dong li tang jing-yong song xin-yu College of Mathematics and Information Science Xinyang Normal University Xinyang 464000 China

The induced matching cover number of a graph G without isolated vertices, denoted by imc(G),is the minimum integer k such that G has k induced matchings {M1,M2,···,Mk}such that,V(M1)∪V(M2)∪···∪V(Mk)covers V(G... 详细信息

关键词： induced matching induced matching cover 3-regular claw-free

Catheter ablation versus medical therapy for atrial fibrillation with prior stroke history:a prospective propensity score-matched cohort study

在线全文

学校读者我要写书评

暂无评论

Journal of Geriatric Cardiology 2023年第10期20卷 707-715,I0006页

作者： Wen-li DAI Zi-Xu ZHAO Chao JIANG liu HE Ke-xin YAO yu-Feng WANG Ming-Yang GAO Yi-Wei LAI jing-Rui ZHANG Ming-Xiao li song ZUO Xue-yuan GUO Ri-Bo tang song-Nan li Chen-Xi JIANG Nian liU De-yong LONG xin DU Cai-Hua SANG Jian-Zeng dong Chang-Sheng MA Department of Cardiology Beijing Anzhen HospitalCapital Medical UniversityNational Clinical Research Center for Cardiovascular DiseasesBeijingChina Heart Health Research Center BeijingChina Department of Cardiovascular Medicine the First Affiliated Hospital of Zhengzhou UniversityZhengzhouChina

BACKGROUND Patients with atrial fibrillation(AF)and prior stroke history have a high risk of cardiovascular events despite anticoagulation *** is unclear whether catheter ablation(CA)has further benefits in these *** AF patients with a previous history of stroke or systemic embolism(SE)from the prospective Chinese Atrial Fibrillation Registry study between August 2011 and December 2020 were included in the *** were matched in a 1:1 ratio to CA or medical treatment(MT)based on propensity *** primary outcome was a composite of all-cause death or ischemic stroke(IS)/*** During a total of 4.1±2.3 years of follow-up,the primary outcome occurred in 111 patients in the CA group(3.3 per 100 person-years)and in 229 patients in the MT group(5.7 per 100 person-years).The CA group had a lower risk of the primary outcome compared to the MT group[hazard ratio(HR)=0.59,95%CI:0.47–0.74,Plity(HR=0.43,95%CI:0.31–0.61,Plity(HR=0.32,95%CI:0.19–0.54,Plinical trials are warranted to confirm the benefits of CA in these patients.

关键词： prospective fibrillation matched

Efficacy and toxicity of capecitabine combined with intensitymodulated radiotherapy after D1/D2 lymph node dissection in patients with gastric cancer

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Oncology 2021年第10期13卷 1532-1543页

作者： xin Wang Wei-Hu Wang Shu-lian Wang yong-Wen song yue-Ping liu yuan tang Ning li Wen-Yang liu Hui Fang Ye-Xiong li dong-Bing Zhao Yihebali Chi lin Yang jing Jin Department of Radiation Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Department of Radiation Oncology Peking University Cancer Hospital and InstituteBeijing 100001China Department of Radiation Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital&Shenzhen HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeShenzhen 518116Guangdong ProvinceChina Department of Pancreatic and Gastric Surgery National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Department of Medical Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China

BACKGROUND Adjuvant chemoradiotherapy(ACRT)with oral capecitabine and intensitymodulated radiotherapy(IMRT)were well tolerated in a phase I study in patients who had undergone partial or total gastrectomy for locally advanced gastric cancer(GC).This phase II study aimed to further determine the efficacy and toxicity of this combination after radical resection and D1/D2 lymph node dissection(LND)for patients with locally advanced *** To further determine the efficacy and toxicity of this combination after radical resection and D1/D2 LND for patients with locally advanced *** Forty patients(median age,53 years;range,24-71 years)with pathologically confirmed adenocarcinoma who underwent D1/D2 LND were included in this *** patients received ACRT comprising IMRT(total irradiation dose:45 Gy delivered in daily 1.8-Gy fractions on 5 d a week over 5 wk)and capecitabine chemotherapy(dose:800 mg/m²twice daily throughout the duration of radiotherapy).The primary study endpoint was disease-free survival(DFS),and the secondary endpoints were overall survival(OS),toxic effects,and treatment *** The 3-year DFS and OS were 66.2%and 75%,*** median time to recurrence was 19.5 mo(range,6.1-68 mo).Peritoneal implantation(n=10)was the most common recurrence pattern,and the lung was the most common site of extra-abdominal metastases(n=5).Nine patients developed grade 3 or 4 toxicities during *** patients discontinued ACRT,while eleven underwent ACRT without receiving the entire course of *** were no treatmentrelated *** The ACRT protocol described herein showed acceptable safety and efficacy for patients with locally advanced GC who received radical gastrectomy and D1/2 LND.

关键词： Gastric cancer Radiotherapy Chemoradiotherapy Clinical trial Phase II

TAX1BP1 and FIP200 orchestrate non-canonical autophagy of p62 aggregates for mouse neural stem cell maintenance

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Zoological Research 2024年第4期45卷 937-950页

作者： Yi-Fu Zhu Rong-Hua yu Shuai Zhou Pei-Pei tang Rui Zhang yu-xin Wu Ran Xu Jia-Ming Wei Ying-Ying Wang Jia-li Zhang Meng-Ke li Xiao-jing Shi yu-Wei Zhang Guang-Zhi liu Rick FThorne Xu dong Zhang Mian Wu song Chen School of life Sciences Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui 230001China Translational Research Institute of Henan Provincial People’s Hospital and People'’s Hospital of Zhengzhou University Tianjian Laboratory of Advanced Biomedical SciencesAcademy of Medical SciencesZhengzhou UniversityZhengzhouHenan 450053China Institute of Medicinal Biotechnology Jiangsu College of NursingHuai’anJiangsu 223300China Laboratory Animal Center Academy of Medical ScienceZhengzhou UniversityZhengzhouHenan 450052China Henan Key Laboratory of Stem cell Differentiation and Modification Henan Provincial People’s HospitalHenan UniversityZhengzhouHenan 450053China School of Biomedical Sciences and Pharmacy The University of NewcastleNSW 2308Australia

Autophagy plays a pivotal role in diverse biological processes,including the maintenance and differentiation of neural stem cells(NSCs).Interestingly,while complete deletion of Fip200 severely impairs NSC maintenance and differentiation,inhibiting canonical autophagy via deletion of core genes,such as Atg5,Atg16l1,and Atg7,or blockade of canonical interactions between FIP200 and ATG13(designated as FIP200-4A mutant or FIP200 KI)does not produce comparable detrimental *** highlights the likely critical involvement of the non-canonical functions of FIP200,the mechanisms of which have remained ***,utilizing genetic mouse models,we demonstrated that FIP200 mediates non-canonical autophagic degradation of p62/sequestome1,primarily via TAX1BP1 in *** deletion of Tax1bp1 in fip200hGFAP conditional knock-in(cKI)mice led to NSC deficiency,resembling the fip200hGFAP conditional knockout(cKO)mouse ***,reintroducing wild-type TAX1BP1 not only restored the maintenance of NSCs derived from tax1bp1-knockout fip200hGFAP cKI mice but also led to a marked reduction in p62 aggregate ***,a TAX1BP1 mutant incapable of binding to FIP200 or NBR1/p62 failed to achieve this ***,conditional deletion of Tax1bp1 in fip200hGFAP cKO mice exacerbated NSC deficiency and p62 aggregate accumulation compared to fip200hGFAP cKO ***,these findings illustrate the essential role of the FIP200-TAX1BP1 axis in mediating the non-canonical autophagic degradation of p62 aggregates towards NSC maintenance and function,presenting novel therapeutic targets for neurodegenerative diseases.

关键词： Non-canonical autophagy TAX1BP1 FIP200 p62 Aggregates Neural stem cell

Exploring an Integrative Therapy for Treating COVID-19:A Randomized Controlled Trial

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Integrative Medicine 2020年第9期26卷 648-655页

作者： WANG Jia-bo WANG Zhong-xia jing jing ZHAO Peng dong jing-hui ZHOU yong-feng YANG Guang NIU Ming ZHAO Xu JIANG Tian-jun Bl jing-feng XU Zhe ZHANG Ping WU Dan BAI Zhao-fang GUO yu-ming yu Si-miao SUN yong-qiang ZHANG Zi-teng ZHAN Xiao-yan li Peng-yan DING Jin-biao ZHAO Peng-fei song Xue-ai tang Jian-yuan HE dong-chu CHEN Zhu QIN En-qiang WANG Rui-lin XIAO Xiao-he China Military Institute of Chinese Medicine The Fifth Medical Center of Chinese PLA General HospitalBeijing(100039)China Integrative Medical Center The Fifth Medical Center of Chinese PLA General HospitalBeijing(100039)China Treatment and Research Center for Infectious Diseases The Fifth Medical Center of Chinese PLA General HospitalBeijing(100039)China National Clinical Research Center for Infectious Diseases Beijing(100039)China Department of Radiology The Fifth Medical Center of Chinese PLA General HospitalBeijing(100039)China Research Center for Clinical and Translational Medicine The Fifth Medical Center of Chinese PLA General HospitalBeijing(100039)China Sichuan Evidence-Based Medicine Center of Traditional Chinese Medicine Affliated Hospital Chengdu University of Traditional Chinese MedicineChengdu(610075)China College of life Science Beijing University of Chinese MedicineBeijing(100029)China Department of Integrated Treatment General Hospital of Central Theater CommandWuhan(430070)China

Objectives:To develop a new Chinese medicine(CM)-based drug and to evaluate its safety and effect for suppressing acute respiratory distress syndrome(ARDS)in COVID-19 ***:A putative ARDS-suppressing drug Keguan-1 was first developed and then evaluated by a randomized,controlled two-arm *** two arms of the trial consist of a control therapy(alpha interferon inhalation,50(x g twice daily;and lopinavir/ritonavir,400 and 100 mg twice daily,respectively)and a testing therapy(control therapy plus Keguan-119.4 g twice daily)by random number table at 1:1 ratio with 24 cases each *** 2-week treatment,adverse events,time to fever resolution,ARDS development,and lung injury on newly diagnosed COVID-19 patients were ***:An analysis of the data from the first 30 participants showed that the control arm and the testing arm did not exhibit any significant differences in terms of adverse *** on this result,the study was expanded to include a total of 48 participants(24 cases each arm).The results show that compared with the control arm,the testing arm exhibited a significant improvement in time to fever resolution(P=0.035),and a significant reduction in the development of ARDS(P=0.048).Conclusions:Keguan-1-based integrative therapy was safe and superior to the standard therapy in suppressing the development of ARDS in COVID-19 patients.(Trial registration *** 04251871 at ***).

关键词： COVID-19 SARS-CoV-2 acute respiratory distress syndrome Chinese medicine

An online coronavirus analysis platform from the National Genomics Data Center

在线全文

学校读者我要写书评

暂无评论

Zoological Research 2020年第6期41卷 705-708页

作者： Zheng Gong Jun-Wei Zhu Cui-Ping li Shuai Jiang li-Na Ma Bi-Xia tang dong Zou Mei-li Chen yu-Bin Sun Shu-Hui song Zhang Zhang jing-Fa Xiao yong-Biao Xue Yi-Ming Bao Zheng-lin Du Wen-Ming Zhao China National Center for Bioinformation&National Genomics Data Center Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China CAS Key Laboratory of Genome Sciences and Information Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

DEAR EDITOR,Since the first reported severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection in December 2019,coronavirus disease 2019(COVID-19)has become a global pandemic,spreading to more than 200 countries and regions *** continued research progress and virus detection,SARS-CoV-2 genomes and sequencing data have been reported and accumulated at an unprecedented rate.

关键词： acute respiratory continued