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Autoantibodies against MMP-7 as a novel diagnostic biomarker in esophageal squamous cell carcinoma

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World Journal of Gastroenterology 2011年第10期17卷 1373-1378页

作者： jing-hua Zhou Bin Zhang Kemp H Kernstine Li Zhong Department of CellBiology College of Life Sciences Hebei University Baoding071400 Hebei Province China Department of Thoracic Surgery City of Hope Medical Center Duarte CA 91766 United States Department of Basic Medical Sciences College of Osteopathic Medicine Western University of Health SciencesPomona CA 91766 United States

AIM： To evaluate the diagnostic values of serum autoan tibodies against matrix metalloproteinase-7 （MMP-7） in patients with esophageal squamous cell carcinoma （ESCC）. METHODS： The MMP-7 cDNA was cloned from ESCC tissues, and MMP-7 was expressed and purified from a prokaryotic system. MMP-7 autoanUbodies were then measured in sera from 50 patients with primary ESCC and 58 risk-matched controls, using a reverse capture enzyme-linked immunosorbent assay （ELISA） in which autoantibodies to MMP-7 bound to the purified MMP-7 proteins. In addition, MMP-7 autoantibody levels in sera from 38 gastric cancer patients and from control serum samples were also tested. RESULTS： The optimum conditions for recombinant MMP-7 protein expression were determined as 0.04 mmol/L Isopropyl-13-D-Thiogalactopyranoside （IPTG）induction at 37℃ for four hours. The levels of serum autoantibodies against MMP-7 were significantly higher in patients with ESCC than in the matched-control samples （OD450 = 1.69 ±0.08 vs OD450 = 1.55 ± 0.10, P 〈 0.001）. The area under the receiver operating character- istic （ROC） curve was 0.87. The sensitivity and specificity for detection of ESCC were 78.0% and 81.0%, respectively, when the OD450 value was greater than 1.65. Although the levels of autoantibodies against MMP-7 were also significantly higher in patients with gastric cancer compared to control samples （OD450 = 1.62± 0.06 vs OD450 = 1.55±0.10, P 〈 0.001）, the diagnostic accuracy was less significant than in ESCC patients. The area of ROC curve was 0.75, whereas the sensitivity and specificity were 60.5% and 71.7%, respectively, when the cut-off value of OD450 was set at 1.60. CONCLUSION： Serum autoantibody levels of MMP-7 may be a good diagnostic biomarker for esophageal squamous cell carcinoma,

关键词： Matrix metalloproteinase-7 Serum autoantibody Esophageal squamous cell carcinoma Gastriccancer Biomarker

In Situ Transmission Electron Microscopy Observations of the Growth Process of Fe3O4–Ag Nanoparticles

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Acta Metallurgica Sinica(English Letters) 2020年第9期33卷 1283-1288页

作者： jing-hua Liu Jin-Jun Liu Qiang Zheng Bao-Ru Bian Juan Du School of Materials Science and Chemical Engineering Ningbo UniversityNingbo 315211China CAS Key Laboratory of Magnetic Materials and Devices Ningbo Institute of Material Technology and EngineeringChinese Academy of SciencesNingbo 315201China Institute of Materials Shanghai UniversityShanghai 200444China

The properties of nanocrystals are highly dependent on their morphology, composition and structure. To obtain full control over their properties, the behavior of nanocrystals under external stimuli, such as heat treatment, needs to be understood. Herein, to in situ observe their microstructure and morphology changes, Fe3O4–Ag heterodimers were selected as a model system. Their structural changes after heat treatment were investigated by in situ transmission electron microscopy. A combination of real-time imaging with elemental analysis enabled observation of the transformation of Fe3O4–Ag heterodimers having a loose interface configuration to those with a Janus structure at the atomic scale after heating from room temperature to 600 °C. After incubation at 600 °C for 32 min, two kinds of Janus structures could be seen, including a clear linear interface in the Fe3O4–Ag heterodimers and a semi-crescent-shaped interface between the Ag and Fe3O4 nanoparticles(NPs). These dynamic observations provide unique insights into NP growth mechanisms, which are essential for understanding and controlling the structure and morphology of nanoparticles.

关键词： In situ TEM Fe3O4–Ag heterodimer Nanoparticle Janus structure Interface

Effect of lymph node micrometastases on prognosis of gastric carcinoma

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World Journal of Gastroenterology 2007年第30期13卷 4122-4125页

作者： Ze-Yu Wu jing-hua Li Wen-hua Zhan Yu-Long He Jin Wan Department of General Surgery Guangdong Provincial People's Hospital Guangzhou 510080 Guangdong Province China Zhongshan Medical College Sun Yat-Sen University Guangzhou 510089 Guangdong Province China Department of Gastrointestinal and Pancreatic Surgery the First Affiliated Hospital Sun Yat-Sen University Guangzhou 510080 Guangdong Province China

AIM： To evaluate the relationship between lymph node micrometastases and prognosis of patients with gastric carcinoma and to evaluate the significance of the new assessment of nodal status in determining the pN categories in the 5th edition of the UICC TNM classification.METHODS： A total of 850 lymph nodes from 30 patients with gastric carcinoma who underwent gastrectomy with lymphadenectomy were assessed by reverse transcription polymerase chain reaction assay in addition to histologic examination. Cytokeratin-20 gene marker was used in this assay.RESULTS： Routine examination by HE staining confirmed metastasis in 233 lymph nodes from 20 patients. All these 233 lymph nodes were oltokeratin-20 positive. Moreover, lymph node micrometastases were detected in an additional 67 lymph nodes in 12 of these 20 patients. Lymph node micrometastases were also detected in t0 lymph nodes from 2 of 10 patients who had no obvious metastases identified by HE staining. Totally, lymph node micrometastases were identified by the reverse transcription polymerase chain reaction assay in 77 （12.5%） lymph nodes from 14 （46.7%） patients with gastric carcinoma. Of 27 patients who underwent curative resection, 7 （25.9%） were up-staged （from Ⅰ B stage to Ⅱ stage in 1 patient, from IB stage to ⅢA stage in 1 patient, from Ⅱ stage to ⅢA stage in 1 patient, from ⅢA stage to ⅢB stage in 1 patient, from ⅢA stage to Ⅳ stage in 1 patient, from ⅢB stage to Ⅳ stage in 2 patients）. In a median follow-up of 32 （range 8-36） too, Kaplan-Meier survival analysis showed significant improvements in median survival （22.86 ± 3.17 mo, 95% CI： 16.64-29.08 mo vs 18.00 ± 7.4 mo, 95% CI： 3.33-32.67 mo） of patients with negative lymph node micrometastases over patients with positive lymph node micrometastases （log-rank, P 〈 0.05）.CONCLUSION： Lymph node micrometastases have a significant impact on the current staging system of gastric carcinoma, and are significant risk factors for prognosis of patients with gast

关键词： Gastric carcinoma Lymph node micrometastases Prognosis Stage

AquaSee: Predict Load and Cooling System Faults of Supercomputers Using Chilled Water Data

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Journal of Computer Science & Technology 2020年第1期35卷 221-230页

作者： Yu-Qi Li Li-Quan Xiao jing-hua Feng Bin Xu Jian Zhang National Supercomputer Center in Tianjin Tianjin 300450China College of Computer National University of Defense TechnologyChangsha 410073China

An analysis of real-world operational data of Tianhe-1A(TH-1A)supercomputer system shows that chilled water data not only can reflect the status of a chiller system but also are related to supercomputer *** study proposes AquaSee,a method that can predict the load and cooling system faults of supercomputers by using chilled water pressure and temperature *** method is validated on the basis of real-world operational data of the TH-1A supercomputer system at the National Supercomputer Center in *** with various compositions are used to construct the prediction model,which is also established using different prediction sequence *** results show that the method that uses a combination of pressure and temperature data performs more effectively than that only consisting of either pressure or temperature *** best inference sequence length is two ***,an anomaly monitoring system is set up by using chilled water data to help engineers detect chiller system anomalies.

关键词： supercomputer chilled water data sensor network load prediction

Lymph node micrometastasis and its correlation with MMP-2 expression in gastric carcinoma

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World Journal of Gastroenterology 2006年第18期12卷 2941-2944页

作者： Ze-Yu Wu jing-hua Li Wen-hua Zhan Yu-Long He Department of Gastrointestinal and Pancreatic Surgery the First Affiliated Hospital Sun Yat-sen University Guangzhou 510080 Guangdong Province China Zhong Shan Medical College Sun Yat-sen University Guangzhou 510089 Guangdong Province China Department of General Surgery Guangdong Provincial People's Hospital Guangzhou 510080 Guangdong Province China

AIM： To examine matrix metalloproteinase-2 （MMP-2） expression in gastric cancer tissues and to evaluate its relationship with lymph node micrometastasis. MATERIALS： The authors studied 850 lymph nodes resected from 30 patients with gastric carcinoma who underwent gastrectomy with lymphadenetomy using reverse transcription polymerase chain reaction （RT-PCR） assay in addition to H-E staining. MMP-2 expression of the tumor tissues was detected by immunohistochemical technique （EliVision^TM plus）. RESULTS： MMP-2 expression was positive in 21 （70%） cases and negative in g （30%） cases. No significant correlations were found between MMP-2 expression and other variables such as age, gender, tumor location, tumor diameter, Lauren classification and lymphatic invasion. In contrast, MMP-2 expression correlated significantly with depth of tumor infiltration （P = 0.022）, lymph node metastasis （P = 0.030） and tumor differentiation （P = 0.043）. Lymph node micrometastases were detected in 77 （12.5%） lymph nodes of 14 （46.7%） gastric carcinoma patients. MMP-2 expression was positive in 12 （85.7%） of the 14 patients with lymph node micrometastasis, and in g （56.3%） of the 16 patients without lymph node micrometastasis （P = 0.118）. CONCLUSIONS： Our results demonstrate that MMP-2 expression has significant correlation with tumor invasion, tumor differentiation and lymph node metastases. MMP-2 expression may be an important biological characteristics and significant prognostic parameter of gastric carcinoma. We also conclude that MMP-2 may participate in the development of lymph node micrometastasis of gastric carcinoma. Further investigations are needed to draw a conclusion.

关键词： Gastric carcinoma Lymph node micrometastasis MMP-2 RT-PCR Immunohistochemistry

Role of infrapatellar fat pad in pathological process of knee osteoarthritis: Future applications in treatment

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World Journal of Clinical Cases 2019年第16期7卷 2134-2142页

作者： Li-Feng Jiang jing-hua Fang Li-Dong Wu Department of Orthopedics Surgery The Second Affiliated Hospital Zhejiang University School of Medicine

It has been found that obese people have a higher proportion in suffering from osteoarthritis (OA),not only in the weight-bearing joints like knee and hip joints,even in non-weight-bearing joints such as hand *** of the reasons is because the large amount of adipose tissue secretes some factors,which can promote the occurrence of *** an important structure of the knee joint,the infrapatellar fat pad (IPFP) is actually a piece of adipose *** aim of this review is to offer a comprehensive view of the anatomy and physiological characteristics of IPFP and its relationship with the pathological process of OA,indicating the important function of IPFP in *** the same time,with the development of adipose derived stem cells in the treatment of OA,owing to its special advantages,the IPFP is becoming a kind of important,minimally invasive fat stem cell source,providing a new approach for the treatment of *** hope that this review will offer an overview of all published data regarding the IPFP and will indicate novel directions for future research.

关键词： Infrapatellar fat pad Osteoarthritis Human mesenchymal stem cells

Treatment of severely calcified coronary artery disease by intravascular lithotripsy primary outcomes and 180-day followup from the Chinese SOLSTICE Trial

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Journal of Geriatric Cardiology 2023年第1期20卷 32-39页

作者： Feng TIAN Shan-Shan ZHOU jing-hua LIU Hui CHEN Zhi-Jun SUN Lian CHEN Qi WANG jing jing Yun-Dai CHEN Department of Cardiology Chinese PLA General HospitalBeijingChina Department of Cardiology Capital Medical University Affiliated Anzhen HospitalBeijingChina Department of Cardiology Capital Medical University Affiliated Beijing Friendship HospitalBeijingChina

OBJECTIVE To assess the safety and effectiveness of intravascular lithotripsy(IVL)treatment for de novo coronary lesion involving severely calcified vessels in a Chinese *** The Clinical Trial of the ShOckwave Coronary IVL System Used to Treat CalcIfied Coronary ArtEries(SOLSTICE)was a prospective,single-arm,multicentre *** to the inclusion criteria,patients with severely calcified lesions were enrolled in the *** was used to perform calcium modification prior to stent *** primary safety endpoint was freedom from major adverse cardiac events(MACEs)at 30 *** primary effectiveness endpoint was procedural success,defined as successful stent delivery with residual stenosis<50% by core lab assessment without in-hospital *** morphological changes of calcium modification were assessed by optical coherence tomography(OCT)before and after IVL *** Patients(n=20)were enrolled at three sites in *** calcification by core lab assessment was present in all lesions,with a mean calcium angle and thickness of 300±51°and 0.99±0.12 mm(by OCT),*** 30-day MACE rate was 5%.Both primary safety and effectiveness endpoints were achieved in 95% of *** final in-stent diameter stenosis was 13.1%±5.7% with no patient had a residual stenosis<50%after *** serious angiographic complications(severe dissection grade D or worse,perforation,abrupt closure,slow flow/no-reflow)observed at any time during the *** imaging demonstrated visible multiplane calcium fracture in 80% of lesions with a mean stent expansion of 95.62%±13.33% at the site of maximum calcification and minimum stent area(MSA)of 5.34±1.64 mm^(2).CONCLUSIONS The initial coronary IVL experience for Chinese operators resulted in high procedural success and low angiographic complications consistent with prior IVL studies,reflecting the relative ease of use of IVL technology.

关键词： coronary intravascular closure

Zn/Fe bimetallic modified Spartina alterniflora-derived biochar heterostructure with superior catalytic performance for the degradation of malachite green

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Biochar 2023年第1期5卷 506-518页

作者： hua jing Lili Ji Zilong Li Zhen Wang Ran Li Kaixuan Ju Ocean College Zhejiang UniversityZhoushanZhejiang 310058China National Marine Facilities Aquaculture Engineering Technology Research Center Zhejiang Ocean UniversityZhoushanZhejiang 316022China Zhejiang Lichen New Material Technology Co. LtdShaoxing 313100China

In this study,the synchronous magnetized carbonization method was utilized for preparing photocatalysis ZnO-Fe@SC heterostructure,which exhibited degradation efficiency 99.14%(60 min)for malachite green(200 mg/L)and could still maintain good performance after 5 *** prepared ZnO-Fe@SC was analyzed using UV-Vis DRS,PL,SEM,TEM,BET,FTIR,XPS and VSM,and LC-MS for degradation *** results indicate that photocatalyst has favorable magnetic properties,chemical stability and low charge carriers(e^(−)/h^(+))recombination *** modification of bimetals enables the composite photocatalyst to enhance the intensity of photogenerated electron ***,quenching experiment revealed that the photo-generated holes(h^(+))and superoxide radicals(·O_(2)^(−))were the dominant active species during the photocatalytic process,which degraded malachite green into small molecules by demethylation,deamination,ring-opening reactions as deducted from LC-MS ***-Fe@SC was prepared using a green,safe,low cost and operable synthetic method,which has a broad market potential in the field of environmental remediation.

关键词： Bimetallic heterostructure Nano-ZnO Green synthesis Photocatalytic degradation

Euler Characteristic and Topological Phase Transition of NUT-Kerr-Newman Black Hole

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Communications in Theoretical Physics 2008年第4期49卷 941-944页

作者： YUE jing-hua YANG Guo-Hong TIAN Li-Jun ZHU Shu Department of Physics Shanghai University Shanghai 200444 China

From the Gauss-Bonnet-Chern theorem, the Euler characteristic of NUT-Kerr-Newman black hole is calculated to be some discrete numbers from 0 to 2. We find that the Bekenstein-Hawking entropy is the largest entropy in topology by taking into account of the relationship between the entropy and the Euler characteristic. The NUT-Kerr- Newman black hole evolves from the torus-like topological structure to the spherical structure with the changes of mass, angular momentum, electric and NUT charges. In this process, the Euler characteristic and the entropy are changed discontinuously, which give the topological aspect of the first-order phase transition of NUT-Kerr-Newman black hole. The corresponding latent heat of the topological phase transition is also obtained. The estimated latent heat of the black hole evolving from the star just lies in the range of the energy of gamma ray bursts.

关键词： euler characteristic entropy NUT-Kerr-Newman black hole killing vector field