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Inter-chromosomal insertions into wild-type chromosomes induced by SCRaMbLE

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Frontiers of Chemical Science and Engineering 2024年第9期18卷 125-135页

作者： Sijie Zhou Junyanrui Li Xichen cui Ying Wang Ying-Jin Yuan Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education) School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China Frontiers Research Institute for Synthetic Biology Tianjin UniversityTianjin 300072China

Genomic rearrangements play a crucial role in shaping biological phenotypic diversity and driving species *** chromosome rearrangement and modification by LoxP-mediated evolution(SCRaMbLE)has been applied to explore large-scale genomic rearrangements,yet it has been observed that these rearrangements occur exclusively in genomic regions containing loxPsym ***,we found that SCRaMbLE of synthetic yeast harboring synthetic chromosome V and X can generate a variety of synthetic segment insertions into wild-type chromosomes,ranging from 1 to 300 ***,it was revealed that the novel insertions impacted the transcriptional level of neighboring regions and affected the production of exemplar pathway of ***,our results improve the understanding of the ability of SCRaMbLE to generate complex structural variations in nonsynthetic regions and provide a potential model to explore genomic transposable events.

关键词： genomic rearrangements synthetic yeast genome SCRaMbLE inter-chromosomal insertions synthetic biology

Biomineralization of soil with crude soybean urease using different calcium salts

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第5期16卷 1788-1798页

作者： Yajie Weng Junjie Zheng Hanjiang Lai Mingjuan cui Xingzhi Ding School of Civil and Hydraulic Engineering Huazhong University of Science and TechnologyWuhan430074China Zijin School of Geology and Mining Fuzhou UniversityFuzhou350108China College of Civil Engineering Fuzhou UniversityFuzhou350116China

Calcium salt is an important contributing factor for calcium-based *** study the effect of calcium salt on soil biomineralization using crude soybean urease,the calcium salts,including the calcium chloride (CaCl_(2)),calcium acetate ((CH_(3)COO)_(2)Ca) and calcium nitrate (Ca(NO_(3))_(2)),were used to prepare the biotreatment solution to carry out the biomineralization tests in this *** series of biomineralization tests in solution and sand column,respectively,were *** electron microscopy (SEM) and X-ray diffraction (XRD) were performed to determine the microscopic characteristics of the precipitated calcium carbonate (CaCO_(3)) *** experimental results indicate that the biomineralization effect is the best for the CaCl2 case,followed by (CH_(3)COO)_(2)Ca,and worst for Ca(NO_(3))_(2) under the test conditions of this study (i.e.1 mol/L of calcium salt-urea).The mechanism for the effect of the calcium salt on the biomineralization of crude soybean urease mainly involves: (1) inhibition of urease activity,and (2) influence on the crystal size and morphology of CaCO_(3).Besides Ca^(2+) ,the anions in solution can inhibit the activity of crude soybean urease,and NO_(3)− has a stronger inhibitory effect on the urease activity compared with both CH_(3)COO^(−) and Cl^(−) .The co-inhibition of Ca^(2+) and NO_(3)− on the activity of urease is the key reason for the worst biomineralization of the Ca(NO_(3))_(2) case in this *** difference in biomineralization between the CaCl_(2) and (CH_(3)COO)_(2) Ca cases is strongly correlated with the crystal morphology of the precipitated CaCO_(3).

关键词： Biomineralization Crude soybean urease Calcium salt Influence mechanism

Estimation of free core nutation parameters and availability of computing options

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Geodesy and Geodynamics 2024年第1期15卷 61-74页

作者： Weiwei Yang Xiaoming cui Jianqiao Xu Qingchao Liu Ming Qin State Key Laboratory of Geodesy and Earth's Dynamics Innovation Academy for Precision Measurement Science and TechnologyChinese Academy of SciencesWuhan 430077China University of Chinese Academy of Sciences Beijing 100049China Hubei Luojia Laboratory Wuhan 430079China

The Earth’s Free Core Nutation(FCN) causes Earth tides and forced nutation with frequencies close to the FCN that exhibit resonance ***-precision superconducting gravimeter(SG) and very long baseline interferometry(VLBI) provide good observation techniques for detecting the FCN ***,some choices in data processing and solution procedures increase the uncertainty of the FCN *** this study,we analyzed the differences and the effectiveness of weight function and ocean tide corrections in the FCN parameter detection using synthetic data,SG data from thirty-one stations,and the 10 celestial pole offset(CPO) *** results show that significant discrepancies are caused by different computing options for a single SG *** stacking method,which results in a variation of0.24-5 sidereal days(SDs) in the FCN period(T) and 10^(3)-10^(4) in the quality factor(Q) due to the selection of the weighting function and the ocean tide model(OTM),can effectively suppress this *** statistical analysis results of synthetic data shows that although different weight choices,while adjusting the proportion of diurnal tidal waves involved,do not significantly improve the accuracy of fitted FCN parameters from gravity *** study evaluated a series of OTMs using the loading correction *** fitting of FCN parameters can be improved by selecting the mean of appropriate OTMs based on the evaluation *** the estimation of the FCN parameters based on the forced nutation,it was found that the weight function P_(1) is more suitable than others,and different CPO series(after 2009) resulted in a difference of 0.4 SDs in the T and of 103 in the *** estimated the FCN parameters for SG(T=430.4±1.5 SDs and Q=1.52×10^(4)±2.5×10^(3)) and for VLBI(T=429.8±0.7 SDs,Q=1.88×10^(4)±2.1×10^(3)).

关键词： Free core nutation Superconducting gravimeter Very long baseline interferometry Weight function Ocean tide model

Cross-linkedα-amylase aggregates on Fe3O4 magnetic nanoparticles modified with polydopamine/polyethyleneimine for efficient hydrolysis of starch

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Particuology 2024年第7期90卷 98-105页

作者： Jiandong cui Xiuming Tang Qingqing Ma Yuyan Chang Qunli Zhang Shiru Jia State Key Laboratory of Food Nutrition and Safety Laboratory of Industrial Fermentation MicrobiologyMinistry of EducationTianjin University of Science and TechnologyTianjin 300457China Key Laboratory of Biomass Chemical Engineering of Ministry of Education Zhejiang UniversityHangzhou 310027China

In this workα-amylase was immobilized on magnetic Fe3O4 nanoparticles with polyethylenimine(PEI)/polydopamine(PDA)coating or 3-aminopropyl triethoxysilane(APTES)for the first time via adsorption–precipitation–*** with the freeα-amylase,the resultant magnetic cross-linkedα-amylase aggregates(PEI/PDA-M-CLEAs and N-M-CLEAs)exhibited excellent thermal and storage stability as well as pH *** storage at 25°C for 60 days,freeα-amylase only retained 60%of its initial activity,while PEI/PDA-M-CLEAs and N-M-CLEAs retained 80%and 78%of their initial activities,***,N-M-CLEAs and PEI/PDA-M-CLEAs showed good *** 6 repeated uses,PEI/PDA-M-CLEAs and N-M-CLEAs still maintained 65%and 62%of their initial activities,***,PEI/PDA-M-CLEAs and N-M-CLEAs exhibited higher starch hydrolysis efficiency than freeα-*** maximum dextrose equivalent(DE)values of starch hydrolysis by PEI/PDA-M-CLEAs and N-M-CLEAs reached 29.24%and 28.79%within 90 min,***,the maximum DE values of starch hydrolysis by the freeα-amylase was only 27.89%even in 150 *** magnetic cross-linkedα-amylase aggregates could be introduced as effective biocatalyst for industrial applications in production of maltose syrups.

关键词： α-amylase Magnetic nanoparticles Functional modification Magnetic cross-linked enzyme aggregates Hydrolysis of starch

Evolutionary Optimization Methods for High-Dimensional Expensive Problems:A Survey

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IEEE/CAA Journal of Automatica Sinica 2024年第5期11卷 1092-1105页

作者： MengChu Zhou Meiji cui Dian Xu Shuwei Zhu Ziyan Zhao Abdullah Abusorrah Department of Electrical and Computer Engineering New Jersey Institute of TechnologyNewarkNJ 07102 USA School of Information and Electronic Engineering Zhejiang Gongshang UniversityHangzhou 310018China School of Intelligent Manufacturing Nanjing University of Science and TechnologyNanjing 210094China Institute of Systems Engineering Macao University of Science and TechnologyMacao 999078China School of Artificial Intelligence and Computer Jiangnan UniversityWuxi 214122China School of Information Science and Engineering Northeastern UniversityShenyang 110819China Center of Research Excellence in Renewable Energy and Power Systems Department of Electrical and Computer EngineeringFaculty of EngineeringKing Abdulaziz UniversityJeddah 21589Saudi Arabia K.A.CARE Energy Research and Innovation Center King Abdulaziz UniversityJeddah 21589Saudi Arabia

Evolutionary computation is a rapidly evolving field and the related algorithms have been successfully used to solve various real-world optimization *** past decade has also witnessed their fast progress to solve a class of challenging optimization problems called high-dimensional expensive problems(HEPs).The evaluation of their objective fitness requires expensive resource due to their use of time-consuming physical experiments or computer ***,it is hard to traverse the huge search space within reasonable resource as problem dimension *** evolutionary algorithms(EAs)tend to fail to solve HEPs competently because they need to conduct many such expensive evaluations before achieving satisfactory *** reduce such evaluations,many novel surrogate-assisted algorithms emerge to cope with HEPs in recent *** there lacks a thorough review of the state of the art in this specific and important *** paper provides a comprehensive survey of these evolutionary algorithms for *** start with a brief introduction to the research status and the basic concepts of ***,we present surrogate-assisted evolutionary algorithms for HEPs from four main *** also give comparative results of some representative algorithms and application ***,we indicate open challenges and several promising directions to advance the progress in evolutionary optimization algorithms for HEPs.

关键词： computer optimization evolutionary

Interplay between microglia and environmental risk factors in Alzheimer's disease

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Neural Regeneration Research 2024年第8期19卷 1718-1727页

作者： Miaoping Zhang Chunmei Liang Xiongjin Chen Yujie Cai Lili cui Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases Affiliated Hospital of Guangdong Medical UniversityZhanjiangGuangdong ProvinceChina

Alzheimer s disease,among the most common neurodegenerative disorders,is chara cterized by progressive cognitive *** present,the Alzheimer’s disease main risk remains genetic ris ks,but major environmental fa ctors are increasingly shown to impact Alzheimer’s disease development and ***,the most important brain immune cells,play a central role in Alzheimer’s disease pathogenesis and are considered environmental and lifestyle"sensors."Factors like environmental pollution and modern lifestyles(e.g.,chronic stress,poor dietary habits,sleep,and circadian rhythm disorde rs)can cause neuroinflammato ry responses that lead to cognitive impairment via microglial functioning and phenotypic ***,the specific mechanisms underlying interactions among these facto rs and microglia in Alzheimer’s disease are ***,we:discuss the biological effects of air pollution,chronic stress,gut micro biota,sleep patterns,physical exercise,cigarette smoking,and caffeine consumption on microglia;consider how unhealthy lifestyle factors influence individual susceptibility to Alzheimer’s disease;and present the neuroprotective effects of a healthy *** intervening and controlling these environmental risk fa ctors at an early Alzheimer’s disease stage,understanding the role of microglia in Alzheimer’s disease development,and to rgeting strategies to to rget microglia,co uld be essential to future Alzheimer’s disease treatments.

关键词： Alzheimer’s disease chronic stress environmental factor gut microbiota microglia particulate matter with diameter<2.5μm

Co-Sharing Waveform Design for Millimeter-Wave Radar Communication Systems

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China Communications 2024年第6期21卷 305-318页

作者： cui Gaofeng He Mengmin Xu Lexi Wang Changheng Wang Weidong School of Electronic Engineering Beijing University of Posts and TelecommunicationsBeijing 100876China Key Laboratory of Universal Wireless Communications Ministry of EducationBeijing University of Posts and TelecommunicationsBeijing 100876China China United Network Communications Corporation Beijing 100048China

Millimeter-wave(mmWave)radar communication has emerged as an important technique for future wireless ***,the interference between the radar signal and communication data is the main issue that should be considered for the joint radar communication *** this paper,a co-sharing waveform(CSW)is proposed to achieve communication and radar sensing *** eliminate the co-interference between the communication and sensing signal,signal splitting and processing methods for communication data demodulation and radar signal processing are given *** results show that the bit error rate(BER)of CSW is close to that of the pure communication ***,the proposed CSW can achieve better performance than the existing waveforms in terms of range and velocity estimation.

关键词： co-sharing waveform millimeter-wave radar communication radar sensing range and velocity estimation

Geometric quality evaluation of three-dimensional printable concrete using computational fluid dynamics

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Frontiers of Structural and Civil Engineering 2024年第7期18卷 963-976页

作者： Weijiu cui Haijun SUN Jiangang ZHOU Sheng WANG Xinyu SHI Yaxin TAO Department of Civil Engineering Qingdao University of TechnologyQingdao 266000China Innovation Institute for Sustainable Maritime Architecture Research and Technology Qingdao University of TechnologyQingdao 266000China Intelligent Construction Lab Qingdao University of TechnologyQingdao 266000China Shandong Zhaojin Industrial Development Co. Ltd.Yantai 264000China Architectural Engineering Institute Weifang Engineering Vocational CollegeWeifang 261000China Qingjian Group Co. Ltd.Qingdao 266000China College of Architecture and Urban Planning Qingdao University of TechnologyQingdao 266000China Department of Structural Engineering and Building Materials Ghent UniversityGhent 9000Belgium Institute of Building Materials ETH ZurichZurich 8064Switzerland

The importance of geometrical control of three dimensional(3D)printable concrete without the support of formwork is widely *** this study,a numerical model based on computational fluid dynamics was developed to evaluate the geometrical quality of a 3D printed *** numerical results were compared,using image analysis,with physical cross-sectional sawn *** influence of printing parameters(printing speed,nozzle height,and nozzle diameter)and the rheological behavior of printed materials(yield stress),on the geometrical quality of one printed layer was *** addition,the yield zone of the printed layer was analyzed,giving insights on the critical factors for geometrical control in 3D concrete *** indicated that the developed model can precisely describe the extrusion process,as well as the cross-sectional quality.

关键词： digital fabrication 3D concrete printing geometric quality computational fluid dynamics printing parameters yield stress

Performance Design of High-Temperature Chloride Salts as Thermal Energy Storage Material

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Journal of Thermal Science 2024年第2期33卷 479-490页

作者： ZHAO Le WANG Jingyao cui Liu LI Baorang DU Xiaoze WU Hongwei Key Laboratory of Power Station Energy Transfer Conversion and System(North China Electric Power University) Ministry of EducationBeijing102206China School of Energy and Power Engineering Lanzhou University of TechnologyLanzhou730050China School of Physics Engineering and Computer ScienceUniversity of HertfordshireHatfieldAL109ABUK

The chloride salts have great potential used as high-temperature thermal energy storage(TES) medium for the concentrated solar power *** this study,LiCl,KCl and CaCl_(2) were selected as energy storage materials in order to further broaden the working temperature of ternary chloride salt and improve its energy storage *** new high-temperature energy storage ternary chloride composed of LiCl,KCl,and CaCl_(2) was developed based on the phase diagram generated by *** components of LiCl-KCl-CaCl_(2) with the mass ratio of 37.85%-53.38%-8.77%,30.90%-13.82%-55.28% and 1.78%-18.61%-79.61% were developed,of which the corresponding melting temperature(T_(m)) 340.93,433.57 and 626.85℃,respectively,was obtained *** that T_(m) of the third group of salts was too high,only the first two groups of salts were *** test showed that the actual melting point was only 0.46% and 1.64% different from the melting point predicted by *** thermal properties of the two ternary chloride salts were also *** solid and liquid-specific heat of ternary salts was determined by DSC using sapphire as the standard *** vapor pressure and decomposition temperature of ternary chloride salts were *** results showed that the vapor pressure of salt 1 was almost constant below 650℃ by ***,the TG results showed that the upper working temperature of salt 1 was 650℃ under the air *** addition,the ternary chloride salts after short-term cycling still exhibited excellent thermal properties,which revealed that these good thermal properties make them have broad application prospects in high-temperature thermal energy storage systems.

关键词： chloride salts thermal energy storages thermal properties thermal stability